JPS6168047A - Blood bag - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Technical Field of the Invention The present invention relates to a blood bag characterized by having an amino acid polymer containing leucine on its inner surface, thereby making it difficult for cells to adhere to the bag.

A blood bag is a bag-like container that stores blood for transfusion.

(b) Description of the prior art Conventionally, blood bags are made of silicone rubber, polyurethane,
Blood bags are manufactured using synthetic polymeric materials such as polyvinyl chloride, but in blood bags made of these materials, cells adhere to the surface of the material and the composition of the blood changes. Therefore, in blood bags, there is a need for materials to which cells do not adhere.

(C) Object of the Invention The object of the present invention is to solve the above problem by using an amino acid polymer containing leucine to provide a blood bag with less cell adhesion.

(d) Structure of the Invention The present inventor has repeatedly conducted various studies on materials that have properties that make it difficult for cells to adhere to them, and has found that amino acid polymers containing leucine have properties that do not allow cells to adhere to a large extent.
They found that it is suitable as a blood bag and completed the present invention.

That is, the blood bag of the present invention can be obtained by molding an amino acid polymer containing leucine into a desired shape, or can be obtained by molding an amino acid polymer containing leucine into a desired shape in advance, and then molding it into a desired shape using other polymeric materials and then adding leucine to its inner surface. Obtained by coating an amino acid polymer containing

Leucine as the constituent material of the amino acid polymer of the present invention and the amino acid as its copolymerization component may be 0-form, L-form, or racemic form, and other amino acids include alanine, glycine, methionine, valine, glutamic acid derivatives, and asparagine. Acid derivatives, lysine derivatives, ornithine derivatives, etc. are used.

The molecular weight of the amino acid polymer is sufficient as long as it forms a film, and the leucine content in the polymer varies depending on the type of amino acid, but is preferably 30 mol% or more.

(e) Examples of the invention Next, the present invention will be explained in more detail with reference to Examples.

Example 1 A mixed solution of benzene and dioxane (7:3) containing a random copolymer of L-leucine and benzyl 1-glutamate (molar ratio of leucine and benzyl glutamate = 7:3) was poured onto a glass plate. The film was spread and air-dried to obtain a film (film thickness: 0.05 m"m). This film was subjected to Soxhlet extraction with ethanol and then dried.

On this film, a culture solution containing human-derived epithelial cells (approximately 1
00,000 pieces/mA) while in contact with the carbon dioxide concentration of 5%,
It was left standing in a room with a humidity of 100% and a temperature of 37°C.

After 17 hours, the film was gently washed with phosphate buffer, and the amount of cells adhering to the film was quantified by nuclear staining.

For comparison, a similar cell adhesion test was conducted using a benzyl glutamate homopolymer film, polyvinyl chloride used in blood bags, and a commercially available cell culture sheet as a standard sample. The cell attachment rate was determined by dividing the amount of cells attached to the film by the amount of cells attached to the standard sample.

The results are shown in Table 1.

Table 1 Example 2 Instead of a random copolymer of L-leucine and L-glutamic acid pencil, a random copolymer of leucine and L-carbobenzoxylidine (the molar ratio of leucine and carbobenzoxylidine) was used. A film was molded in the same manner as in Example 1, except that a ratio of 7:3) was used, and a cell adhesion experiment was conducted. The results are shown in Table 2.

Table 2 Example 3 A leucine-containing amino acid polymer was applied to the inner surface of a glass container (c) After drying, it was removed from the glass container to obtain a bag-shaped container.

Example 4 In a random copolymer of "-leucine and "-benzyl glutamate, the respective molar ratio was 85:15.
．． A film having a ratio of 50:50.30:70 was obtained in the same manner as in Example 1.

Example 5 In a copolymer of L-leucine and L-carbobenzoxylidine, the molar ratio of each is 85:15.50:
A film of 50.30:70 was obtained in the same manner as in Example 1.

Example 6 Bory L-leucine was dissolved in hot benzene and a film was obtained in the same manner as in Example 1.

Example 7 A block copolymer consisting of L-leucine and benzyl L-glutamate was prepared by the N-carboxyamino acid anhydride method using hexamethylene diamine as an initiator, a mixture of benzene and dioxane, and a solvent (benzene and dioxane). The composition of this block copolymer was (benzyl glutamate (18%)).
))-(leucine (64%))-(benzyl glutamate (18%)). ). This copolymer was dissolved in hot benzene, and a film was obtained in the same manner as in Example 1.

Example 8 A block copolymer film was obtained in the same manner as in Example 7 using L-carbobenzoxylidine instead of benzyl L-glutamate.

(f> Effects of the Invention As explained above, the present invention suppresses cell adhesion by molding an amino acid polymer containing leucine on the inner surface of a blood bag that requires less cell adhesion, and It is possible to obtain this blood bag in any shape.

designated agent Director, Product Science Research Institute, Agency of Industrial Science and Technology Atsushi Takahashi

Claims (1)

[Claims] (1) Blood bag with an amino acid polymer containing leucine on its inner surface