JPS61234775A - Bed material for cell culture - Google Patents

Abstract

PURPOSE:The titled bed material having high adhesion to cell, having a benzyl glutamate-containing amino acid copolymer on the surface. CONSTITUTION:Benzyl glutamate is copolymerized with an amino acid (e.g., methyl glutamate, etc.) in a molar ratio of 1:1 to give a benzyl glutamate- containing amino acid copolymer, which is molded into sheet, yarn, beads, hollow yarn, etc., or another material is molded into the aimed form and the copolymer is applied to the molded article to give the aimed bed material.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Technical Field of the Invention The present invention is used to measure proliferation by attaching a large amount of amino acid containing benzyl glutamate. In general, animal cells do not grow in suspension, but only grow when attached to solid surfaces. Therefore, in order to culture such cells, a solid material, that is, a bed material, to which a large amount of cells can adhere is required.

(b) Description of the prior art Conventionally, cell culture bedding materials have been made using synthetic polymer materials such as polystyrene and polymethacrylate.
Cell culture floor materials made of these materials are difficult for cells to adhere to.

(C) Purpose of the Invention The object of the present invention is to solve the above-mentioned problem by using an amino acid copolymer containing benzyl glutamate, thereby providing a bed material for cell culture with high cell adhesion.

The present inventors have discovered that the material has the property of adhering to cells, making it suitable as a cell culture flooring material, and have completed the present invention.

That is, the cell culture bed material of the present invention can be obtained by molding an amino acid copolymer containing benzyl glutamate into a desired shape such as a sheet, fiber, bead, or hollow fiber.
Alternatively, it can be obtained by first molding into a desired shape using another material and then applying the amino acid copolymer containing benzyl glutamate of the present invention to the surface thereof.

Benzyl glutamate as a constituent material of the amino acid copolymer of the present invention and the amino acid as its copolymerization component may be 0-form, L-form, or racemic form, and other amino acids include alanine, glycine, methionine, valine, glutamic acid derivatives, and asparagine. Acid derivatives, lysine derivatives, ornithine derivatives, etc. Next, the present invention will be explained in more detail with reference to Examples.

Example 1 A 1,2-dichloroethane solution of a copolymer of methyl glutamate and benzyl L-glutamate (molar ratio of methyl glutamate to benzyl glutamate = 1:1) was cast onto a glass plate and air-dried. film (thickness: 0)
．． 05 mm) was obtained. This film was subjected to Soxhlet extraction with ethanol and then dried. A culture solution containing human-derived epithelial cells (approximately 100,000 cells/m cell) was placed on the film at 37°C at a carbon dioxide concentration of 5% and humidity of 100%.
I left it in my room. After 17 hours, the film was gently washed with phosphate buffer, and the amount of cells adhering to the film was quantified by nuclear staining.

2. For comparison, the cell adhesion rate was determined by dividing the amount of cells adhering to the methyl glutamate homopolymer skin or film by the amount of cells adhering to the standard sample.

The results are shown in Table 1.

Table 1 From this table it is clear that this copolymer attaches more cells than the control sample polymethacrylate.

Example 2 Instead of a 1,2-dichloroethane solution of a copolymer of methyl L-glutamate and benzyl L-glutamate,
Table 2 From this table, except that a dioxane solution of a copolymer of carbobenzoxy-L-lysine and benzyl L-glutamate (molar ratio of carbobenzoxylidine to benzyl glutamate = 7:3) was used. It is clear that the copolymer attaches more cells than the control sample, polymethacrylate.

Example 3 Dioxane solution of the copolymer of Example 2 (temperature: about 0.5%)
) Glass beads (about 0.5 mm in diameter) were immersed in the glass beads, and then the solvent was removed and dried to obtain a bead-shaped cell culture bed material having a copolymer of carbobenzoxylidine and benzyl glutamate on the surface. Ta.

(f) Effects of the Invention As explained above, the present invention uses an amino acid copolymer containing benzyl glutamate as a cell culture flooring material that requires high cell adhesion. By performing one molding, it is possible to increase the amount of cells attached.

designated agent Director, Institute of Product Utilization, Agency of Industrial Science and Technology Kyouji Takahashi

Claims (1)

[Claims] (1) A bed material for cell culture having an amino acid copolymer containing benzyl glutamate on its surface.