JPS6168058A - Diaphragm for preventing adhesion - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Technical Field of the Invention The present invention relates to a diaphragm for preventing adhesion, which is characterized by having an amino acid polymer containing leucine on its surface, thereby making it difficult for cells to adhere to it.

The adhesion prevention diaphragm is used to prevent post-operative tissue (
This is used to prevent the incision (incision) from adhesion.

(b) Description of Prior Art Conventionally, adhesion prevention diaphragms have been manufactured using synthetic polymer materials such as silicone rubber, polyurethane, and polytetrafluoroethylene. In adhesion prevention diaphragms made of these materials, cells adhere to the surface of the material and cannot be removed. Therefore, a material to which cells do not adhere is required for the adhesion prevention diaphragm.

(c> Purpose of the Invention The object of the present invention is to solve the above-mentioned problem by using an amino acid polymer containing leucine to provide a diaphragm for preventing adhesion with low cell adhesion.

(d) Structure of the Invention The present inventor has repeatedly conducted various studies on materials that have properties that make it difficult for cells to adhere to them, and has found that amino acid polymers containing leucine have properties that do not allow cells to adhere to a large extent.
It was discovered that the present invention is suitable as a diaphragm for preventing adhesion, and the present invention was completed.

That is, the adhesion-preventing diaphragm of the present invention can be obtained by molding an amino acid polymer containing leucine into the desired film shape, or by molding it into a film shape from another polymeric material in advance and then applying leucine to the surface. Obtained by coating an amino acid polymer containing

Leucine as the constituent material of the amino acid polymer of the present invention and the amino acid as its copolymerization component may be 0-form, L-form, or racemic form, and other amino acids include alanine, glycine, methionine, valine, glutamic acid derivatives, and aspartic acid. derivatives, lysine derivatives, ornithine derivatives, etc. are used.

Amine i5! The molecular weight of the polymer is sufficient as long as it forms a film, and the leucine content in the polymer varies depending on the type of amino acid, but is preferably 30 mol% or more.

(e) Examples of the invention Next, the present invention will be explained in more detail with reference to Examples.

Example 1 Copolymer of L-leucine and benzyl L-glutamate (molar ratio of leucine and benzyl glutamate = 7:3
) A mixed solution of benzene and dioxane (7:3) was cast on a glass plate and air-dried to form a film (0.05 mm thick).
) was obtained. This film was subjected to Soxhlet extraction with ethanol and then dried.

On this film, a culture solution containing human-derived epithelial cells (approximately 1
00,000 pieces/mλ) in contact with the carbon dioxide concentration of 5%,
It was left standing in a room with a humidity of 100% and a temperature of 37°C.

After 17 hours, the film was lightly washed with phosphate buffer, and the amount of cells adhering to the film was determined by nuclear staining. For comparison, a similar cell adhesion test was conducted using the benzyl glutamate homopolymer film, the silicone rubber used in the adhesion prevention diaphragm, and a commercially available cell culture sheet as a standard sample.

The cell attachment rate was determined by dividing the amount of cells attached to the film by the amount of cells attached to the standard sample.

The results are shown in Table 1.

Table 1 Example 2 Copolymer of L-leucine and L-carbobenzoxylidine instead of copolymer of L-leucine and benzyl L-glutamate Molar ratio of leucine and carbobenzoxylidine = 7: A film was molded in the same manner as in Example 1 except that 3) was used, and a cell adhesion experiment was conducted. The results are shown in Table 2.

Table 2 Example 3 In a copolymer of L-leucine and benzyl L-glutamate, the molar ratio of each is 85:15.50
: 50.30 : A film of 70 was obtained in the same manner as in Example 1.

Example 4 In a copolymer of L-leucine and L-carbobenzoxylidine, the molar ratio of each is 85:15.50
: 50.30 : A film of 70 was obtained in the same manner as in Example 1.

Example 5 Poly L-leucine was dissolved in hot benzene and a film was obtained in the same manner as in Example 1.

Example 6 A block copolymer consisting of L-leucine and benzyl monoglutamate was prepared by the N-carboxyamino acid anhydride method using hexamethylene diamine as an initiator, and a mixed solvent of benzene and dioxane (volume ratio of benzene and dioxane) was prepared using hexamethylene diamine as an initiator. =19: Synthesized in 1) (The composition of this block copolymer is (benzyl glutamate (18%)) -
(Leucine (64%)>-(benzyl glutamate (1)
8%)). ). This copolymer was dissolved in hot benzene, and a film was obtained in the same manner as in Example 1.

Example 7 A block copolymer film was obtained in the same manner as in Example 6 using L-carbobenzoxylidine instead of benzyl L-glutamate.

(f) Effects of the Invention As explained above, the present invention provides a diaphragm (X) for preventing adhesion, which requires low cell adhesion, by molding an amino acid polymer containing leucine on the surface. It is possible to suppress the adhesion of adhesion and obtain this adhesion-preventing diaphragm in any shape.

Claims (1)

[Claims] (1) Adhesion prevention diaphragm with an amino acid polymer containing leucine on its surface