JPS6168049A - Blood bag - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

Detailed Description of the Invention (a) Technical Field of the Invention The present invention relates to a blood bag that is characterized by having a 1 m polyglutamic conductor surface-treated with amino alcohol on its inner surface to prevent cells from adhering to it. It is a real thing.

A blood bag is a bag-like container that stores blood for transfusion.

(b) Description of the prior art Conventionally, blood bags are made of silicone rubber, polyurethane,
Blood bags are manufactured using synthetic polymeric materials such as polyvinyl chloride, but in blood bags made of these materials, cells adhere to the surface of the material and the composition of the blood changes. Therefore, in blood bags, there is a need for materials to which cells do not adhere.

(C) Object of the Invention The object of the present invention is to solve the above problem by providing a blood bag with less cell adhesion by using a polyglutamic acid derivative surface-treated with amine alcohol.

(d> Structure of the Invention The present inventor has conducted various studies on materials that have properties that make it difficult for cells to adhere to them, and has found that polyglutamic acid derivatives surface-treated with amine alcohol have properties that do not allow cells to adhere significantly. They found that it is suitable as a blood bag, and completed the present invention.

That is, the blood bag of the present invention can be obtained by molding a polyglutamic acid derivative into a desired tubular shape and then surface-treating the shaped product with amino alcohol, or by shaping the polyglutamic acid derivative into the desired shape in advance with other polymeric materials. After molding, a polyglutamic acid derivative is applied to the inner surface of the mold, and the surface is then treated with amino alcohol.

The polyglutamic acid derivative of the present invention may be 0-form, L-form, or racemic form, and esters of aliphatic hydrocarbons and aromatic hydrocarbons such as methyl polyglutamate and benzyl polyglutamate are used as the polyglutamic acid derivative. The molecular weight of the polyglutamic acid derivative is sufficient as long as it forms a film.

As the amino alcohol, ethanolamine, aminopropanol, and alkylene glycol derivatives such as H2NC2H40C2H40H are used. The surface treatment time is appropriately selected depending on the type and concentration of the amino alcohol and the type of polyglutamic acid derivative, but in any case, the surface treatment should ensure that the contact angle of the inner surface of the bag-shaped container with water is 45° or less. is desirable.

(e) Examples of the invention Next, the present invention will be roughly explained in detail with reference to Examples.

Example 1 A dioxane solution of benzyl glutamate homopolymer was cast onto a glass plate and air-dried to form a film (thickness: approximately 0.05 m).
m) was obtained. This film was subjected to Soxhlet extraction with ethanol and then dried.

This film was heated at 55°C in 3-amino-1-propanol for 4 hours.
The amithrisis reaction was allowed to take place for a while. After the reaction, the film was washed with ethanol. This film was immersed in hot water of 80° C. or higher for 8 hours or more and dried to obtain a surface-treated film. Table 1 shows the contact angle of this surface treated film with water. For comparison,
An example of polyvinyl chloride is also given.

Table 1 Example 2 A surface treated film was obtained in the same manner as in Example 1 except that a dichloroethane solution of methyl glutamate homopolymer was used instead of the dioxane solution of benzyl glutamate homopolymer.

Example 3 A culture solution containing human-derived epithelial cells (approximately 100,000 cells/mQ) was placed on the film obtained in Example 1 at a temperature of 5% carbon dioxide concentration, 100% humidity, and 31°C. I left it in the room. 1
After 7 hours, the film was gently washed with phosphate buffer, and the m of cells adhering to the film was quantified by nuclear staining. For comparison, a similar cell adhesion test was conducted using polyvinyl chloride used in blood bags and a commercially available cell culture sheet as a standard sample. The amount of cells attached to the membrane,
The cell attachment rate was determined by dividing by the amount of cells attached to the standard sample, and the results are shown in Table 2.

Table 2 Example 4 4 hours at 60°C in H2NC2H40C2Ha○H instead of 4 hours at 55°C in 3-amino-1-propatur
A surface treated film was obtained in the same manner as in Example 1 except that the time treatment was performed.

Example 5 After applying a polyglutamic acid derivative to the inner surface of a glass container and drying it, it was heated at 55°C in 3-amino-1-propatol.
After treatment for 4 hours with ethanol and washing with ethanol,
It was immersed in hot water at 0° C. or higher for 8 hours or more, and after drying, it was removed from the glass container to obtain a bag-shaped container.

(f) Effects of the Invention As explained above, the present invention prevents cell adhesion by molding a polyglutamic acid derivative surface-treated with amino alcohol on the inner surface of a blood bag that requires less cell adhesion. It is possible to obtain this blood bag in any desired shape.

designated agent Director, Product Science Research Institute, Agency of Industrial Science and Technology 1r　6　　Otsu/r? 1

Claims (1)

[Claims] (1) Blood bag with polyglutamic acid derivative surface treated with amino alcohol on the inner surface