JPS61234774A - Red material for cell culture - Google Patents

Red material for cell culture

Info

Publication number
JPS61234774A
JPS61234774A JP60077324A JP7732485A JPS61234774A JP S61234774 A JPS61234774 A JP S61234774A JP 60077324 A JP60077324 A JP 60077324A JP 7732485 A JP7732485 A JP 7732485A JP S61234774 A JPS61234774 A JP S61234774A
Authority
JP
Japan
Prior art keywords
cell culture
film
dried
polymethionine
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP60077324A
Other languages
Japanese (ja)
Other versions
JPH0553472B2 (en
Inventor
Norihiko Minoura
憲彦 箕浦
Seiichi Aiba
誠一 相羽
Yukihiko Fujiwara
冨士原　行彦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP60077324A priority Critical patent/JPS61234774A/en
Publication of JPS61234774A publication Critical patent/JPS61234774A/en
Publication of JPH0553472B2 publication Critical patent/JPH0553472B2/ja
Granted legal-status Critical Current

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  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To provide a bed material for cell culture having high cell agglutination property, by using a polymethionine as the surface material. CONSTITUTION:A polymethionine (D-isomer or racemic compound having a molecular weight to enable the formation of a film) is dissolved e.g. in trichloroethylene, and the solution is cast on a glass plate, etc., and dried in air to form a coating film. The film is extracted with a Soxlet extractor and dried to obtain a sheet. As an alternative method, the solution is applied to the surface of other material formed to a desired shape and dried by removing the solvent to obtain the object bed material for cell culture.

Description

【発明の詳細な説明】 （ａ）発明の技術分野 を′多量に付着させて増殖をはかるために使用するもの
である。一般に、動物細胞は浮遊状態では増殖せず、固
体表面に付着してのみ増殖する性質を有している。した
がってそのような細胞を培養するためには細胞を多量に
付着する固体、つまり床材が必要となる。DETAILED DESCRIPTION OF THE INVENTION (a) Technical Field of the Invention: Used for measuring proliferation by attaching a large amount. In general, animal cells do not grow in suspension, but only grow when attached to solid surfaces. Therefore, in order to culture such cells, a solid material, that is, a bed material, to which a large amount of cells can adhere is required.

（ｂ）従来技術の説明 従来、細胞培養用床材はポリスチレン、ポリメタクリレ
ートなどの合成高分子材料を用いて作製されているが、
これらの材料を用いた細胞培養用、床材では細胞が付着
しにくい。(b) Description of the prior art Conventionally, cell culture bedding materials have been made using synthetic polymer materials such as polystyrene and polymethacrylate.
Cells do not easily adhere to flooring materials for cell culture using these materials.

（Ｃ）発明の目的 本発明は上記の問題をポリメチオニンを用いることによ
り、細胞付着性の高い細胞培養用床材を提供することを
目的とする。(C) Purpose of the Invention The object of the present invention is to solve the above-mentioned problem by using polymethionine, thereby providing a cell culture bed material with high cell adhesion.

（ｄ）発明の構成 本発明者は細胞の付着しやすい性質を有する材即ち、本
発明の細胞培養用床材は、ポリメチオニンをシート状、
繊維状、ビーズ状、中空糸状などの目的とする形状に成
型して得るか、あるいはあらかじめ他の材料で目的とす
る形状に成型した後、その表面に本発明のポリメチオニ
ンを塗布して得る。(d) Structure of the Invention The present inventor has discovered that the cell culture flooring material of the present invention is a material having properties to which cells easily adhere, i.e., the cell culture flooring material of the present invention.
It can be obtained by molding into a desired shape such as a fiber, bead, or hollow fiber, or by molding into a desired shape using another material in advance and then applying the polymethionine of the present invention to the surface thereof.

本発明のポリメチオニンは０体、１体、ラセミ体でもよ
く、その分子量はその皮膜が形成される程度であればよ
い。The polymethionine of the present invention may be 0-isomer, 1-isomer, or racemic, and its molecular weight may be sufficient as long as it forms a film.

（ｅ）発明の実施例 次に本発明を実施例によりさらに詳細に説明する。(e) Examples of the invention Next, the present invention will be explained in more detail with reference to Examples.

実施例１ ポリーＬ−メチオニンのトリクロルエチレン溶液をガラ
ス板上に流延し、風乾して皮膜（膜厚的０．０５ｍｍ）
を得た。この皮膜をエタノールでソ１７時“間後、皮膜
をリン酸緩衝液でかるく洗浄し、皮膜上に付着している
細胞の量を核染色法により定量した。Example 1 A trichloroethylene solution of poly-L-methionine was cast on a glass plate and air-dried to form a film (film thickness: 0.05 mm).
I got it. After soaking this film in ethanol for 17 hours, the film was lightly washed with phosphate buffer, and the amount of cells adhering to the film was quantified by nuclear staining.

比較のため、Ｉｌｌ胞培養用床材に使用されているポリ
メタクリレートを用いて、同様の細胞付着試験を行った
。For comparison, a similar cell adhesion test was conducted using polymethacrylate, which is used as a bed material for Ill cell culture.

皮膜に付着した細胞の量を標準試料に付着した細胞の量
で割ることにより、細胞付着率を求めた。The cell attachment rate was determined by dividing the amount of cells attached to the film by the amount of cells attached to the standard sample.

その結果を第１表に示す。The results are shown in Table 1.

第　　１　　表 この表から、ポリーＬ−メチオニンは対照試料であるポ
リメタクリレートより多量の細胞を付着する゛ことが明
らかである。Table 1 From this table it is clear that poly L-methionine attached more cells than the control sample polymethacrylate.

一浸・潰し、その後溶媒を除去、乾燥して、ポリーＬ−
メチオニンを表面に有するビーズ状細胞培養用床材を得
た。Poly L-
A bead-shaped cell culture bed material having methionine on the surface was obtained.

（ｆ）発明の効果 本発明は以上説明したように、細胞付着性の高いことを
必要とする細胞培養用床材において、ポリメチオニンを
表面に成型することにより細胞の付着量を増すことが可
能である。(f) Effect of the invention As explained above, the present invention makes it possible to increase the amount of cells attached by molding polymethionine on the surface of a cell culture flooring material that requires high cell adhesion. It is.

指定代理人 工業技術院製品科学研究所長 高橋教司designated agent Director, Product Science Research Institute, Agency of Industrial Science and Technology Kyouji Takahashi

Claims (1)

【特許請求の範囲】[Claims] （１）ポリメチオニンを表面に有する細胞培養用床材。(1) A bed material for cell culture having polymethionine on its surface.
JP60077324A 1985-04-11 1985-04-11 Red material for cell culture Granted JPS61234774A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP60077324A JPS61234774A (en) 1985-04-11 1985-04-11 Red material for cell culture

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP60077324A JPS61234774A (en) 1985-04-11 1985-04-11 Red material for cell culture

Publications (2)

Publication Number Publication Date
JPS61234774A true JPS61234774A (en) 1986-10-20
JPH0553472B2 JPH0553472B2 (en) 1993-08-10

Family

ID=13630753

Family Applications (1)

Application Number Title Priority Date Filing Date
JP60077324A Granted JPS61234774A (en) 1985-04-11 1985-04-11 Red material for cell culture

Country Status (1)

Country Link
JP (1) JPS61234774A (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0521663U (en) * 1991-09-09 1993-03-23 株式会社シマノ Oscillating mechanism of spinning reel

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59201849A (en) * 1983-04-30 1984-11-15 工業技術院長 Polyamino acid film composite body

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59201849A (en) * 1983-04-30 1984-11-15 工業技術院長 Polyamino acid film composite body

Also Published As

Publication number Publication date
JPH0553472B2 (en) 1993-08-10

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Legal Events

Date Code Title Description
EXPY Cancellation because of completion of term