JPS60243097A - 単クローン抗体の分離方法 - Google Patents

単クローン抗体の分離方法

Info

Publication number
JPS60243097A
JPS60243097A JP60096563A JP9656385A JPS60243097A JP S60243097 A JPS60243097 A JP S60243097A JP 60096563 A JP60096563 A JP 60096563A JP 9656385 A JP9656385 A JP 9656385A JP S60243097 A JPS60243097 A JP S60243097A
Authority
JP
Japan
Prior art keywords
hybridoma
antibody
protein
salt
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP60096563A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0588116B2 (enExample
Inventor
チヤールズ　ルイズ，ジユニア
ジトカ　ベラ　オランダー
ウイリアム　ロバート　トルバート
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Monsanto Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Co filed Critical Monsanto Co
Publication of JPS60243097A publication Critical patent/JPS60243097A/ja
Publication of JPH0588116B2 publication Critical patent/JPH0588116B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y10S530/809—Fused cells, e.g. hybridoma

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Peptides Or Proteins (AREA)
JP60096563A 1984-05-08 1985-05-07 単クローン抗体の分離方法 Granted JPS60243097A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US608283 1984-05-08
US06/608,283 US4533496A (en) 1984-05-08 1984-05-08 Method of isolating monoclonal antibodies from hybridoma cultures

Publications (2)

Publication Number Publication Date
JPS60243097A true JPS60243097A (ja) 1985-12-03
JPH0588116B2 JPH0588116B2 (enExample) 1993-12-21

Family

ID=24435801

Family Applications (1)

Application Number Title Priority Date Filing Date
JP60096563A Granted JPS60243097A (ja) 1984-05-08 1985-05-07 単クローン抗体の分離方法

Country Status (6)

Country Link
US (1) US4533496A (enExample)
EP (1) EP0162034B1 (enExample)
JP (1) JPS60243097A (enExample)
AU (1) AU579628B2 (enExample)
CA (1) CA1236409A (enExample)
DE (1) DE3580513D1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010516773A (ja) * 2007-01-22 2010-05-20 ジェネンテック， インコーポレイテッド 多価電解質沈殿物及びタンパク質の精製

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6051120A (ja) * 1983-08-31 1985-03-22 Chemo Sero Therapeut Res Inst 単純ヘルペスサブユニットワクチン
US4933435A (en) * 1989-04-05 1990-06-12 Bioprobe International Antibody purification process
US5580774A (en) * 1989-07-31 1996-12-03 Eli Lilly And Company Chimeric antibodies directed against a human glycoprotein antigen
ZA923679B (en) * 1991-06-21 1993-01-27 Pro Soma Sarl Diagnostic method
EP0583980A1 (en) * 1992-08-20 1994-02-23 Eli Lilly And Company Method for generating monoclonal antibodies from rabbits
JPH06323712A (ja) 1993-04-20 1994-11-25 E I Du Pont De Nemours & Co 霧化した極低温液滴の閉じ込め帯域を用いて凍結粒子を製造する方法および装置
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8163886B2 (en) 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
US8569464B2 (en) 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
US20120177610A1 (en) 2007-09-19 2012-07-12 Kieu Hoang Manufacturing and Purification Processes of Complex Protein found in Fraction IV to make a separated Apo, Transferrin , and Alpha 1 Anti strepsin (A1AT) or A combined Transferrin / Apo/Human Albumin/A1AT and all new found proteins
WO2009151514A1 (en) 2008-06-11 2009-12-17 Millipore Corporation Stirred tank bioreactor
ES2749232T3 (es) 2008-12-16 2020-03-19 Emd Millipore Corp Reactor de tanque agitado y procedimiento
SG10201804385YA (en) 2010-05-17 2018-06-28 Emd Millipore Corp Stimulus responsive polymers for the purification of biomolecules
TW201335181A (zh) * 2012-01-31 2013-09-01 Kieu Hoang ５５種新發現的蛋白質之序列及其應用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5928492A (ja) * 1982-07-19 1984-02-15 モンサント・コンパニ− 細胞培養系よりの血しよう蛋白質の分離

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3555001A (en) * 1969-05-29 1971-01-12 Monsanto Co Process for the fractionation of plasma and serum using water-insoluble polyelectrolytes containing diloweralkylaminoloweralkylimide groups
NL7708005A (nl) * 1976-07-22 1978-01-24 Monsanto Co Werkwijze voor de bereiding van serumalbumine.
JPS5929622A (ja) * 1982-08-10 1984-02-16 Meiji Seika Kaisha Ltd モノクロ−ナル抗体、その製造法およびその用途
US4469630A (en) * 1983-11-25 1984-09-04 J. T. Baker Chemical Co. Purification of monoclonal antibodies

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5928492A (ja) * 1982-07-19 1984-02-15 モンサント・コンパニ− 細胞培養系よりの血しよう蛋白質の分離

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010516773A (ja) * 2007-01-22 2010-05-20 ジェネンテック， インコーポレイテッド 多価電解質沈殿物及びタンパク質の精製

Also Published As

Publication number Publication date
EP0162034A3 (en) 1987-05-20
CA1236409A (en) 1988-05-10
AU579628B2 (en) 1988-12-01
AU4203185A (en) 1985-11-14
EP0162034A2 (en) 1985-11-21
US4533496A (en) 1985-08-06
JPH0588116B2 (enExample) 1993-12-21
DE3580513D1 (de) 1990-12-20
EP0162034B1 (en) 1990-11-14

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