JPS60195436A - 流れ血球計数装置 - Google Patents
流れ血球計数装置Info
- Publication number
- JPS60195436A JPS60195436A JP60003917A JP391785A JPS60195436A JP S60195436 A JPS60195436 A JP S60195436A JP 60003917 A JP60003917 A JP 60003917A JP 391785 A JP391785 A JP 391785A JP S60195436 A JPS60195436 A JP S60195436A
- Authority
- JP
- Japan
- Prior art keywords
- prism
- cell
- light
- cells
- flow path
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000000601 blood cell Anatomy 0.000 title description 4
- 210000004027 cell Anatomy 0.000 claims description 87
- 239000007788 liquid Substances 0.000 claims description 30
- 230000003287 optical effect Effects 0.000 claims description 29
- 238000005286 illumination Methods 0.000 claims description 19
- 239000002699 waste material Substances 0.000 claims description 13
- 238000004820 blood count Methods 0.000 claims description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 5
- 238000001514 detection method Methods 0.000 claims description 4
- 230000001427 coherent effect Effects 0.000 claims description 3
- 239000000377 silicon dioxide Substances 0.000 claims description 2
- 235000012239 silicon dioxide Nutrition 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 1
- 230000033764 rhythmic process Effects 0.000 claims 1
- 238000000034 method Methods 0.000 description 8
- 230000004075 alteration Effects 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000002245 particle Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000005540 biological transmission Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 238000000576 coating method Methods 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N argon Substances [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- -1 argon ion Chemical class 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 239000005350 fused silica glass Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000004941 influx Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 239000007320 rich medium Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1497—Particle shape
Landscapes
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Dispersion Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
- Optical Measuring Cells (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US586162 | 1984-03-05 | ||
| US06/586,162 US4545677A (en) | 1984-03-05 | 1984-03-05 | Prismatic beam expander for light beam shaping in a flow cytometry apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60195436A true JPS60195436A (ja) | 1985-10-03 |
| JPH0258585B2 JPH0258585B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1990-12-10 |
Family
ID=24344558
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60003917A Granted JPS60195436A (ja) | 1984-03-05 | 1985-01-12 | 流れ血球計数装置 |
Country Status (3)
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62102705U (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1985-12-19 | 1987-06-30 | ||
| JPS62293143A (ja) * | 1986-06-12 | 1987-12-19 | Rion Co Ltd | 微粒子測定装置 |
| JPH01196536A (ja) * | 1988-02-01 | 1989-08-08 | Canon Inc | 粒子解析装置 |
| JPH02289808A (ja) * | 1989-04-28 | 1990-11-29 | Olympus Optical Co Ltd | 照明光学系 |
| US7417734B2 (en) | 2004-04-30 | 2008-08-26 | Bay Bioscience Kabushiki Kaisha | System and process for sorting biological particles |
| US7576909B2 (en) | 1998-07-16 | 2009-08-18 | Imra America, Inc. | Multimode amplifier for amplifying single mode light |
| JP2013527929A (ja) * | 2010-05-03 | 2013-07-04 | バイオ−ラッド・ラボラトリーズ・インコーポレーテッド | フローサイトメーターにおいて複数のレーザービームを合波するための光学コンバイナ |
| CN106383082A (zh) * | 2015-10-14 | 2017-02-08 | 北京信息科技大学 | 一种流式细胞仪无液路情况的光路调整装置及方法 |
| JP2024119909A (ja) * | 2016-03-17 | 2024-09-03 | ベクトン・ディキンソン・アンド・カンパニー | ハイスループット蛍光フローサイトメーターを用いる細胞選別 |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1292628C (en) * | 1986-02-12 | 1991-12-03 | James Phinazee Sutton Iii | In situ particle size measuring device |
| JPS63214668A (ja) * | 1987-03-03 | 1988-09-07 | Hitachi Ltd | 細胞測定方法 |
| JPH07104349B2 (ja) * | 1987-04-11 | 1995-11-13 | 株式会社日立製作所 | 細胞測定法 |
| US5132548A (en) * | 1990-09-14 | 1992-07-21 | High Yield Technology | High sensitivity, large detection area particle sensor for vacuum applications |
| US5489977A (en) * | 1993-08-11 | 1996-02-06 | Texaco Inc. | Photomeric means for monitoring solids and fluorescent material in waste water using a falling stream water sampler |
| EP2264427B1 (en) * | 1997-01-31 | 2017-05-03 | Xy, Llc | Optical apparatus with focussing reflector for converging radiation onto a flow of particles, and related method of analysis |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| US6154276A (en) * | 1998-02-23 | 2000-11-28 | The Regents Of The University Of California | Waveguide detection of right-angle-scattered light in flow cytometry |
| US6542304B2 (en) | 1999-05-17 | 2003-04-01 | Toolz, Ltd. | Laser beam device with apertured reflective element |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| EP2258174A3 (en) | 2000-05-09 | 2012-06-13 | Xy, Llc | High purity x-chromosome bearing and y-chromosome bearing populations of spermatozoa |
| WO2002043486A1 (en) | 2000-11-29 | 2002-06-06 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
| US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| CN1674780A (zh) | 2002-08-01 | 2005-09-28 | Xy公司 | 低压精子分离系统 |
| US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
| MXPA05001654A (es) | 2002-08-15 | 2005-10-18 | Xy Inc | Citometro de flujo de alta resolucion. |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| US20040061853A1 (en) * | 2002-09-27 | 2004-04-01 | Blasenheim Barry J. | Prism-based flow cytometry excitation optics |
| JP3891925B2 (ja) * | 2002-12-03 | 2007-03-14 | ベイバイオサイエンス株式会社 | 生物学的粒子の情報を得る装置 |
| EP2305172B1 (en) | 2003-03-28 | 2016-05-11 | Inguran, LLC | Apparatus and methods for providing sex-sorted animal sperm |
| AU2004242121B2 (en) | 2003-05-15 | 2010-06-24 | Xy, Llc. | Efficient haploid cell sorting for flow cytometer systems |
| EP1730266A2 (en) | 2004-03-29 | 2006-12-13 | Monsanto Technology, LLC | Sperm suspensions for sorting into x or y chromosome-bearing enriched populations |
| BRPI0513685A (pt) | 2004-07-22 | 2008-05-13 | Monsanto Technology Llc | processo para enriquecimento de uma população de células de esperma |
| US7110192B2 (en) * | 2005-01-12 | 2006-09-19 | Dako Denmark A/S | System and method for a composite lens for a flow cytometer |
| EP1951742A4 (en) * | 2005-10-27 | 2011-06-01 | Life Technologies Corp | OPTOELECTRONIC SEPARATION OF BIOMOLECULES |
| US7869038B2 (en) * | 2007-08-15 | 2011-01-11 | Malvern Instruments, Ltd. | Broad-range spectrometer |
| CN103091211B (zh) * | 2011-11-03 | 2017-05-17 | 深圳迈瑞生物医疗电子股份有限公司 | 荧光检测系统和细胞分析仪 |
| EP2943778B1 (en) | 2013-01-09 | 2020-10-28 | The Regents of The University of California | Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation |
| ES2959506T3 (es) | 2014-03-18 | 2024-02-26 | Univ California | Citómetro de flujo paralelo que usa multiplexación por radiofrecuencia, y método |
| AU2016339956B2 (en) | 2015-10-13 | 2021-07-01 | Omega Biosystems Incorporated | Multi-modal fluorescence imaging flow cytometry system |
| US10935485B2 (en) | 2016-05-12 | 2021-03-02 | Bd Biosciences | Fluorescence imaging flow cytometry with enhanced image resolution |
| EP3513167B1 (en) | 2016-09-13 | 2023-05-24 | Becton, Dickinson and Company | Flow cytometer with optical equalization |
| CN106918544A (zh) * | 2017-03-29 | 2017-07-04 | 中国科学院苏州生物医学工程技术研究所 | 一种基于荧光激发光源的流式分析仪系统 |
| AU2018302191B2 (en) | 2017-07-19 | 2021-05-13 | Inguran, Llc | Method and system incorporating beam shaping optics and beam stabilization |
| US11648551B2 (en) | 2017-12-12 | 2023-05-16 | Essenlix Corporation | Sample manipulation and assay with rapid temperature change |
| EP3997439A4 (en) | 2019-07-10 | 2023-07-19 | Becton, Dickinson and Company | RECONFIGURABLE INTEGRATED CIRCUITS TO ADJUST CELL SORTING CLASSIFICATION |
| US11513058B2 (en) | 2020-05-19 | 2022-11-29 | Becton, Dickinson And Company | Methods for modulating an intensity profile of a laser beam and systems for same |
| EP4172592B1 (en) | 2020-06-26 | 2025-07-16 | Becton, Dickinson and Company | Dual excitation beams for irradiating a sample in a flow stream and methods for using same |
| CN112433384A (zh) * | 2020-12-16 | 2021-03-02 | 桂林优利特医疗电子有限公司 | 一种用于流式细胞仪的多色激光光路系统 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5326996A (en) * | 1976-08-26 | 1978-03-13 | Tokyo Keiki Kk | Compound dielectric body |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3826364A (en) * | 1972-05-22 | 1974-07-30 | Univ Leland Stanford Junior | Particle sorting method and apparatus |
| US4084881A (en) * | 1975-10-21 | 1978-04-18 | Canon Kabushiki Kaisha | Light beam scanning device |
| DE2732272C2 (de) * | 1977-07-16 | 1979-07-05 | Deutsches Krebsforschungszentrum Stiftung Des Oeffentlichen Rechts, 6900 Heidelberg | Verfahren und Vorrichtung zur Fluoreszenzanalyse von gefärbten Partikeln, insbesondere biologischen Zellen |
| US4498766A (en) * | 1982-03-25 | 1985-02-12 | Becton, Dickinson And Company | Light beam focal spot elongation in flow cytometry devices |
-
1984
- 1984-03-05 US US06/586,162 patent/US4545677A/en not_active Expired - Lifetime
-
1985
- 1985-01-12 JP JP60003917A patent/JPS60195436A/ja active Granted
- 1985-03-02 DE DE19853507407 patent/DE3507407A1/de active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5326996A (en) * | 1976-08-26 | 1978-03-13 | Tokyo Keiki Kk | Compound dielectric body |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62102705U (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1985-12-19 | 1987-06-30 | ||
| JPS62293143A (ja) * | 1986-06-12 | 1987-12-19 | Rion Co Ltd | 微粒子測定装置 |
| JPH01196536A (ja) * | 1988-02-01 | 1989-08-08 | Canon Inc | 粒子解析装置 |
| JPH02289808A (ja) * | 1989-04-28 | 1990-11-29 | Olympus Optical Co Ltd | 照明光学系 |
| US7576909B2 (en) | 1998-07-16 | 2009-08-18 | Imra America, Inc. | Multimode amplifier for amplifying single mode light |
| US7417734B2 (en) | 2004-04-30 | 2008-08-26 | Bay Bioscience Kabushiki Kaisha | System and process for sorting biological particles |
| US7639358B2 (en) | 2004-04-30 | 2009-12-29 | Bay Bioscience Kabushiki Kaisha | System and process for sorting biological particles |
| JP2013527929A (ja) * | 2010-05-03 | 2013-07-04 | バイオ−ラッド・ラボラトリーズ・インコーポレーテッド | フローサイトメーターにおいて複数のレーザービームを合波するための光学コンバイナ |
| CN106383082A (zh) * | 2015-10-14 | 2017-02-08 | 北京信息科技大学 | 一种流式细胞仪无液路情况的光路调整装置及方法 |
| JP2024119909A (ja) * | 2016-03-17 | 2024-09-03 | ベクトン・ディキンソン・アンド・カンパニー | ハイスループット蛍光フローサイトメーターを用いる細胞選別 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0258585B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1990-12-10 |
| US4545677A (en) | 1985-10-08 |
| DE3507407C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1988-11-24 |
| DE3507407A1 (de) | 1985-09-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |