JPS59192099A - 微生物菌数の測定法 - Google Patents
微生物菌数の測定法Info
- Publication number
- JPS59192099A JPS59192099A JP58064308A JP6430883A JPS59192099A JP S59192099 A JPS59192099 A JP S59192099A JP 58064308 A JP58064308 A JP 58064308A JP 6430883 A JP6430883 A JP 6430883A JP S59192099 A JPS59192099 A JP S59192099A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- microorganisms
- solution
- methyl
- amino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 244000005700 microbiome Species 0.000 title claims abstract description 47
- 238000005259 measurement Methods 0.000 title abstract description 7
- 239000000243 solution Substances 0.000 claims abstract description 54
- 239000000725 suspension Substances 0.000 claims abstract description 25
- 239000012895 dilution Substances 0.000 claims abstract description 13
- 238000010790 dilution Methods 0.000 claims abstract description 13
- 102000004190 Enzymes Human genes 0.000 claims abstract description 4
- 108090000790 Enzymes Proteins 0.000 claims abstract description 4
- 230000003301 hydrolyzing effect Effects 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 106
- 239000000203 mixture Substances 0.000 claims description 6
- YPLZVJKSYBUKBU-UHFFFAOYSA-N 3-amino-4-methylchromen-2-one Chemical compound C1=CC=CC2=C1OC(=O)C(N)=C2C YPLZVJKSYBUKBU-UHFFFAOYSA-N 0.000 claims 2
- 235000013305 food Nutrition 0.000 abstract description 14
- GLNDAGDHSLMOKX-UHFFFAOYSA-N coumarin 120 Chemical compound C1=C(N)C=CC2=C1OC(=O)C=C2C GLNDAGDHSLMOKX-UHFFFAOYSA-N 0.000 abstract description 9
- HSHNITRMYYLLCV-UHFFFAOYSA-N 4-methylumbelliferone Chemical class C1=C(O)C=CC2=C1OC(=O)C=C2C HSHNITRMYYLLCV-UHFFFAOYSA-N 0.000 abstract description 8
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 230000007062 hydrolysis Effects 0.000 abstract description 2
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 2
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N coumarin Chemical compound C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 abstract 2
- 238000005558 fluorometry Methods 0.000 abstract 2
- 229960000956 coumarin Drugs 0.000 abstract 1
- 235000001671 coumarin Nutrition 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 14
- 238000006243 chemical reaction Methods 0.000 description 14
- 239000007788 liquid Substances 0.000 description 13
- 241000233866 Fungi Species 0.000 description 11
- 239000004471 Glycine Substances 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 239000002609 medium Substances 0.000 description 7
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 6
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 229940041514 candida albicans extract Drugs 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 235000012033 vegetable salad Nutrition 0.000 description 5
- 239000012138 yeast extract Substances 0.000 description 5
- 230000006378 damage Effects 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000002537 cosmetic Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 238000001802 infusion Methods 0.000 description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 description 3
- 239000013028 medium composition Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000002351 wastewater Substances 0.000 description 3
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 2
- IJJWOSAXNHWBPR-HUBLWGQQSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-n-(6-hydrazinyl-6-oxohexyl)pentanamide Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)NCCCCCC(=O)NN)SC[C@@H]21 IJJWOSAXNHWBPR-HUBLWGQQSA-N 0.000 description 2
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 2
- 239000004278 EU approved seasoning Substances 0.000 description 2
- 240000008415 Lactuca sativa Species 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- 102000016943 Muramidase Human genes 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 235000013361 beverage Nutrition 0.000 description 2
- 239000001768 carboxy methyl cellulose Substances 0.000 description 2
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 2
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 2
- 229960005091 chloramphenicol Drugs 0.000 description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 239000010840 domestic wastewater Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 235000013312 flour Nutrition 0.000 description 2
- 235000011194 food seasoning agent Nutrition 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 229960000274 lysozyme Drugs 0.000 description 2
- 235000010335 lysozyme Nutrition 0.000 description 2
- 239000004325 lysozyme Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 239000001965 potato dextrose agar Substances 0.000 description 2
- 235000012045 salad Nutrition 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 102220549062 Low molecular weight phosphotyrosine protein phosphatase_C13S_mutation Human genes 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 1
- 241000674789 Nicator Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 241000228153 Penicillium citrinum Species 0.000 description 1
- DYUQAZSOFZSPHD-UHFFFAOYSA-N Phenylpropanol Chemical compound CCC(O)C1=CC=CC=C1 DYUQAZSOFZSPHD-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 241001148470 aerobic bacillus Species 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 125000003710 aryl alkyl group Chemical group 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-N phosphoric acid Substances OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 125000003367 polycyclic group Chemical group 0.000 description 1
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229950008882 polysorbate Drugs 0.000 description 1
- 229940068968 polysorbate 80 Drugs 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000013324 preserved food Nutrition 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000012898 sample dilution Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000021055 solid food Nutrition 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- KUOGTDMKRNWKQW-UHFFFAOYSA-H trimagnesium hexachloride Chemical compound [Mg+2].[Mg+2].[Mg+2].[Cl-].[Cl-].[Cl-].[Cl-].[Cl-].[Cl-] KUOGTDMKRNWKQW-UHFFFAOYSA-H 0.000 description 1
- 238000002525 ultrasonication Methods 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
- C12Q2334/20—Coumarin derivatives
- C12Q2334/22—4-Methylumbelliferyl, i.e. beta-methylumbelliferone, 4MU
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/20—Coumarin derivatives
- C12Q2337/22—7-Amino-4-methylcoumarin, i.e. AMC, MCA
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58064308A JPS59192099A (ja) | 1983-04-12 | 1983-04-12 | 微生物菌数の測定法 |
| EP84302431A EP0122148B1 (en) | 1983-04-12 | 1984-04-10 | Method of measuring the number of cells of microorganisms |
| DE8484302431T DE3468125D1 (en) | 1983-04-12 | 1984-04-10 | Method of measuring the number of cells of microorganisms |
| US06/599,632 US4675289A (en) | 1983-04-12 | 1984-04-12 | Method of measuring the number of eumycete cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58064308A JPS59192099A (ja) | 1983-04-12 | 1983-04-12 | 微生物菌数の測定法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59192099A true JPS59192099A (ja) | 1984-10-31 |
| JPH0472517B2 JPH0472517B2 (enExample) | 1992-11-18 |
Family
ID=13254477
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58064308A Granted JPS59192099A (ja) | 1983-04-12 | 1983-04-12 | 微生物菌数の測定法 |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US4675289A (enExample) |
| EP (1) | EP0122148B1 (enExample) |
| JP (1) | JPS59192099A (enExample) |
| DE (1) | DE3468125D1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01201161A (ja) * | 1987-11-27 | 1989-08-14 | Radiometer Corporate Dev Ltd | 体液のアッセイ方法及びキット |
| JPH0411899A (ja) * | 1990-04-27 | 1992-01-16 | Shimadzu Corp | 生物体の計数法 |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8416044D0 (en) * | 1984-06-22 | 1984-07-25 | Unilever Plc | Carrying out microchemical and microbiological tests |
| DE3429823A1 (de) * | 1984-08-14 | 1986-02-27 | Merck Patent Gmbh, 6100 Darmstadt | Mittel und verfahren zum nachweis antimikrobiell wirksamer substanzen |
| US5089395A (en) * | 1985-02-27 | 1992-02-18 | University Of Cincinnati | Viable microorganism detection by induced fluorescence |
| WO1986005206A1 (en) * | 1985-02-27 | 1986-09-12 | University Of Cincinnati | Viable microorganism detection by induced fluorescence |
| US4812409A (en) * | 1986-01-31 | 1989-03-14 | Eastman Kodak Company | Hydrolyzable fluorescent substrates and analytical determinations using same |
| US4803157A (en) * | 1986-01-31 | 1989-02-07 | Eastman Kodak Company | Hydrolyzable fluorescent substrates for phosphatases and analytical use thereof |
| US5429933A (en) * | 1986-06-30 | 1995-07-04 | Edberg; Stephen C. | Detection of first generation environmental sourced microbes in an environmentally-derived sample |
| US4925789A (en) * | 1986-06-30 | 1990-05-15 | Edberg Stephen C | Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material |
| US4952495A (en) * | 1987-06-08 | 1990-08-28 | Eastman Kodak Company | Hydrolyzable compounds which release electron transfer agents and analytical use of same |
| FR2628531B1 (fr) * | 1988-03-08 | 1992-10-02 | Chemunex Sa | Procede de recherche, de detection specifique et de numeration de micro-organismes, installation pour la mise en oeuvre dudit procede |
| US5700655A (en) * | 1995-11-14 | 1997-12-23 | Idexx Laboratories, Inc. | Method for quantification of biological material in a sample |
| US5985594A (en) * | 1995-11-14 | 1999-11-16 | Idexx Laboratories, Inc. | Method for quantification of biological material in a sample |
| US7122338B2 (en) * | 1995-11-14 | 2006-10-17 | Biocontrol Systems, Inc. | Method for quantification of biological material in a sample |
| GB9609024D0 (en) * | 1996-05-01 | 1996-07-03 | Idg Uk Ltd | Compounds |
| US5854011A (en) * | 1996-12-19 | 1998-12-29 | Idexx Laboratories Incorporated | Method and components for the detection of yeasts and/or molds in a sample |
| WO1999019510A1 (en) | 1997-10-10 | 1999-04-22 | President And Fellows Of Harvard College | Surface-bound, double-stranded dna protein arrays |
| DE102004002607A1 (de) * | 2004-01-15 | 2005-08-04 | Beiersdorf Ag | Visualisierung von Sonnenschutzmitteln auf der Haut |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5468290A (en) * | 1977-11-10 | 1979-06-01 | Seikagaku Kogyo Co Ltd | Method of measuring toxin in bacterium by original fluorescent substance |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3451893A (en) * | 1966-09-27 | 1969-06-24 | Litton Systems Inc | Method of rapidly detecting microorganisms |
| US4146604A (en) * | 1973-05-31 | 1979-03-27 | Block Engineering, Inc. | Differential counting of leukocytes and other cells |
| JPS53105477A (en) * | 1977-02-26 | 1978-09-13 | Ajinomoto Co Inc | 7-glycylprolylamono-4-methylcoumarine |
| US4215047A (en) * | 1977-06-06 | 1980-07-29 | Ajinomoto Company Incorporated | 7-(Arginylamino)-4-methylcoumarins |
| DE3117241A1 (de) * | 1981-04-30 | 1982-11-18 | Ajinomoto Co., Inc., Tokyo | Schnellverfahren zum nachweis oder zur bestimmung von mikroorganismen |
| JPS6064797A (ja) * | 1983-09-20 | 1985-04-13 | Seiko Instr & Electronics Ltd | プレス装置 |
-
1983
- 1983-04-12 JP JP58064308A patent/JPS59192099A/ja active Granted
-
1984
- 1984-04-10 EP EP84302431A patent/EP0122148B1/en not_active Expired
- 1984-04-10 DE DE8484302431T patent/DE3468125D1/de not_active Expired
- 1984-04-12 US US06/599,632 patent/US4675289A/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5468290A (en) * | 1977-11-10 | 1979-06-01 | Seikagaku Kogyo Co Ltd | Method of measuring toxin in bacterium by original fluorescent substance |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01201161A (ja) * | 1987-11-27 | 1989-08-14 | Radiometer Corporate Dev Ltd | 体液のアッセイ方法及びキット |
| JPH0411899A (ja) * | 1990-04-27 | 1992-01-16 | Shimadzu Corp | 生物体の計数法 |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0122148A1 (en) | 1984-10-17 |
| EP0122148B1 (en) | 1987-12-16 |
| DE3468125D1 (en) | 1988-01-28 |
| US4675289A (en) | 1987-06-23 |
| JPH0472517B2 (enExample) | 1992-11-18 |
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