JPS59144719A - ヒトインタ―ロイキン２活性を有するポリペプチド - Google Patents
ヒトインタ―ロイキン２活性を有するポリペプチドInfo
- Publication number
- JPS59144719A JPS59144719A JP58041358A JP4135883A JPS59144719A JP S59144719 A JPS59144719 A JP S59144719A JP 58041358 A JP58041358 A JP 58041358A JP 4135883 A JP4135883 A JP 4135883A JP S59144719 A JPS59144719 A JP S59144719A
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- gene
- base sequence
- base
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 207
- 108010002350 Interleukin-2 Proteins 0.000 title claims abstract description 151
- 102000000588 Interleukin-2 Human genes 0.000 title claims abstract description 148
- 239000000126 substance Substances 0.000 title abstract description 8
- 210000004027 cell Anatomy 0.000 claims abstract description 210
- 238000000034 method Methods 0.000 claims abstract description 108
- 241000588724 Escherichia coli Species 0.000 claims abstract description 39
- 239000013598 vector Substances 0.000 claims abstract description 31
- 210000004962 mammalian cell Anatomy 0.000 claims abstract description 23
- 229930006000 Sucrose Natural products 0.000 claims abstract description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 10
- 239000005720 sucrose Substances 0.000 claims abstract description 10
- 238000000432 density-gradient centrifugation Methods 0.000 claims abstract description 9
- 108020004414 DNA Proteins 0.000 claims description 126
- 230000000694 effects Effects 0.000 claims description 56
- 108020004511 Recombinant DNA Proteins 0.000 claims description 53
- 239000002773 nucleotide Substances 0.000 claims description 44
- 125000003729 nucleotide group Chemical group 0.000 claims description 44
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 32
- 229920001184 polypeptide Polymers 0.000 claims description 30
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 30
- 108091008146 restriction endonucleases Proteins 0.000 claims description 22
- 210000004698 lymphocyte Anatomy 0.000 claims description 18
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 16
- 241000588722 Escherichia Species 0.000 claims description 14
- 238000004519 manufacturing process Methods 0.000 claims description 14
- 210000001236 prokaryotic cell Anatomy 0.000 claims description 14
- 108091026890 Coding region Proteins 0.000 claims description 13
- 210000003527 eukaryotic cell Anatomy 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 11
- 238000005194 fractionation Methods 0.000 claims description 8
- 241000282693 Cercopithecidae Species 0.000 claims description 7
- 108091034057 RNA (poly(A)) Proteins 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 230000003362 replicative effect Effects 0.000 claims description 4
- 101710128836 Large T antigen Proteins 0.000 claims description 3
- 108010087924 alanylproline Proteins 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 13
- 108010025306 histidylleucine Proteins 0.000 claims 4
- WKPXXXUSUHAXDE-SRVKXCTJSA-N Arg-Pro-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C(N)N)C(O)=O WKPXXXUSUHAXDE-SRVKXCTJSA-N 0.000 claims 3
- HDBOEVPDIDDEPC-CIUDSAMLSA-N Ser-Lys-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(O)=O HDBOEVPDIDDEPC-CIUDSAMLSA-N 0.000 claims 3
- 210000004408 hybridoma Anatomy 0.000 claims 3
- CNKBMTKICGGSCQ-ACRUOGEOSA-N (2S)-2-[[(2S)-2-[[(2S)-2,6-diamino-1-oxohexyl]amino]-1-oxo-3-phenylpropyl]amino]-3-(4-hydroxyphenyl)propanoic acid Chemical compound C([C@H](NC(=O)[C@@H](N)CCCCN)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)C1=CC=CC=C1 CNKBMTKICGGSCQ-ACRUOGEOSA-N 0.000 claims 2
- QNNBHTFDFFFHGC-KKUMJFAQSA-N Asn-Tyr-Lys Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(=O)N)N)O QNNBHTFDFFFHGC-KKUMJFAQSA-N 0.000 claims 2
- XLLSMEFANRROJE-GUBZILKMSA-N Cys-Leu-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)[C@H](CS)N XLLSMEFANRROJE-GUBZILKMSA-N 0.000 claims 2
- FGPLUIQCSKGLTI-WDSKDSINSA-N Gly-Ser-Glu Chemical compound NCC(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCC(O)=O FGPLUIQCSKGLTI-WDSKDSINSA-N 0.000 claims 2
- QVFGXCVIXXBFHO-AVGNSLFASA-N Leu-Glu-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O QVFGXCVIXXBFHO-AVGNSLFASA-N 0.000 claims 2
- CNUPMMXDISGXMU-CIUDSAMLSA-N Met-Cys-Glu Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(O)=O)C(O)=O CNUPMMXDISGXMU-CIUDSAMLSA-N 0.000 claims 2
- XDGFFEZAZHRZFR-RHYQMDGZSA-N Met-Leu-Thr Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XDGFFEZAZHRZFR-RHYQMDGZSA-N 0.000 claims 2
- YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 claims 2
- KBVJZCVLQWCJQN-KKUMJFAQSA-N Phe-Leu-Asn Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O KBVJZCVLQWCJQN-KKUMJFAQSA-N 0.000 claims 2
- RMJZWERKFFNNNS-XGEHTFHBSA-N Pro-Thr-Ser Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(O)=O RMJZWERKFFNNNS-XGEHTFHBSA-N 0.000 claims 2
- YMEXHZTVKDAKIY-GHCJXIJMSA-N Ser-Asn-Ile Chemical compound CC[C@H](C)[C@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CO)C(O)=O YMEXHZTVKDAKIY-GHCJXIJMSA-N 0.000 claims 2
- GZBKRJVCRMZAST-XKBZYTNZSA-N Ser-Glu-Thr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O GZBKRJVCRMZAST-XKBZYTNZSA-N 0.000 claims 2
- NADLKBTYNKUJEP-KATARQTJSA-N Ser-Thr-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(O)=O NADLKBTYNKUJEP-KATARQTJSA-N 0.000 claims 2
- UGFSAPWZBROURT-IXOXFDKPSA-N Thr-Phe-Cys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CS)C(=O)O)N)O UGFSAPWZBROURT-IXOXFDKPSA-N 0.000 claims 2
- BURPTJBFWIOHEY-UWJYBYFXSA-N Tyr-Ala-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 BURPTJBFWIOHEY-UWJYBYFXSA-N 0.000 claims 2
- 108010038633 aspartylglutamate Proteins 0.000 claims 2
- 101150082072 14 gene Proteins 0.000 claims 1
- 101150064522 60 gene Proteins 0.000 claims 1
- 101150061292 83 gene Proteins 0.000 claims 1
- 101150077691 89 gene Proteins 0.000 claims 1
- 101710102786 ATP-dependent leucine adenylase Proteins 0.000 claims 1
- FFZJHQODAYHGPO-KZVJFYERSA-N Ala-Pro-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@H](C)N FFZJHQODAYHGPO-KZVJFYERSA-N 0.000 claims 1
- LSMDIAAALJJLRO-XQXXSGGOSA-N Ala-Thr-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(O)=O LSMDIAAALJJLRO-XQXXSGGOSA-N 0.000 claims 1
- POZKLUIXMHIULG-FDARSICLSA-N Arg-Trp-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CC1=CNC2=CC=CC=C21)NC(=O)[C@H](CCCN=C(N)N)N POZKLUIXMHIULG-FDARSICLSA-N 0.000 claims 1
- CQMQJWRCRQSBAF-BPUTZDHNSA-N Asn-Arg-Trp Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(=O)N)N CQMQJWRCRQSBAF-BPUTZDHNSA-N 0.000 claims 1
- BVLIJXXSXBUGEC-SRVKXCTJSA-N Asn-Asn-Tyr Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O BVLIJXXSXBUGEC-SRVKXCTJSA-N 0.000 claims 1
- PNHQRQTVBRDIEF-CIUDSAMLSA-N Asn-Leu-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(=O)N)N PNHQRQTVBRDIEF-CIUDSAMLSA-N 0.000 claims 1
- AWXDRZJQCVHCIT-DCAQKATOSA-N Asn-Pro-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CC(N)=O AWXDRZJQCVHCIT-DCAQKATOSA-N 0.000 claims 1
- XTMZYFMTYJNABC-ZLUOBGJFSA-N Asn-Ser-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)N)N XTMZYFMTYJNABC-ZLUOBGJFSA-N 0.000 claims 1
- JNCRAQVYJZGIOW-QSFUFRPTSA-N Asn-Val-Ile Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O JNCRAQVYJZGIOW-QSFUFRPTSA-N 0.000 claims 1
- RQHLMGCXCZUOGT-ZPFDUUQYSA-N Asp-Leu-Ile Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O RQHLMGCXCZUOGT-ZPFDUUQYSA-N 0.000 claims 1
- BDWIZLQVVWQMTB-XKBZYTNZSA-N Cys-Glu-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CS)N)O BDWIZLQVVWQMTB-XKBZYTNZSA-N 0.000 claims 1
- AOZBJZBKFHOYHL-AVGNSLFASA-N Cys-Glu-Tyr Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O AOZBJZBKFHOYHL-AVGNSLFASA-N 0.000 claims 1
- BUZMZDDKFCSKOT-CIUDSAMLSA-N Glu-Glu-Glu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O BUZMZDDKFCSKOT-CIUDSAMLSA-N 0.000 claims 1
- MUSGDMDGNGXULI-DCAQKATOSA-N Glu-Glu-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CCC(O)=O MUSGDMDGNGXULI-DCAQKATOSA-N 0.000 claims 1
- QJCKNLPMTPXXEM-AUTRQRHGSA-N Glu-Glu-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CCC(O)=O QJCKNLPMTPXXEM-AUTRQRHGSA-N 0.000 claims 1
- DVLZZEPUNFEUBW-AVGNSLFASA-N Glu-His-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CC1=CN=CN1)NC(=O)[C@H](CCC(=O)O)N DVLZZEPUNFEUBW-AVGNSLFASA-N 0.000 claims 1
- IVGJYOOGJLFKQE-AVGNSLFASA-N Glu-Leu-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)O)N IVGJYOOGJLFKQE-AVGNSLFASA-N 0.000 claims 1
- HZISRJBYZAODRV-XQXXSGGOSA-N Glu-Thr-Ala Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O HZISRJBYZAODRV-XQXXSGGOSA-N 0.000 claims 1
- DGKBSGNCMCLDSL-BYULHYEWSA-N Gly-Ile-Asn Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)CN DGKBSGNCMCLDSL-BYULHYEWSA-N 0.000 claims 1
- MMFKFJORZBJVNF-UWVGGRQHSA-N His-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CN=CN1 MMFKFJORZBJVNF-UWVGGRQHSA-N 0.000 claims 1
- YAALVYQFVJNXIV-KKUMJFAQSA-N His-Leu-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CN=CN1 YAALVYQFVJNXIV-KKUMJFAQSA-N 0.000 claims 1
- RQJUKVXWAKJDBW-SVSWQMSJSA-N Ile-Ser-Thr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N RQJUKVXWAKJDBW-SVSWQMSJSA-N 0.000 claims 1
- IBMVEYRWAWIOTN-UHFFFAOYSA-N L-Leucyl-L-Arginyl-L-Proline Natural products CC(C)CC(N)C(=O)NC(CCCN=C(N)N)C(=O)N1CCCC1C(O)=O IBMVEYRWAWIOTN-UHFFFAOYSA-N 0.000 claims 1
- RCFDOSNHHZGBOY-UHFFFAOYSA-N L-isoleucyl-L-alanine Natural products CCC(C)C(N)C(=O)NC(C)C(O)=O RCFDOSNHHZGBOY-UHFFFAOYSA-N 0.000 claims 1
- IBMVEYRWAWIOTN-RWMBFGLXSA-N Leu-Arg-Pro Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N1CCC[C@@H]1C(O)=O IBMVEYRWAWIOTN-RWMBFGLXSA-N 0.000 claims 1
- MLTRLIITQPXHBJ-BQBZGAKWSA-N Leu-Asn Chemical compound CC(C)C[C@H](N)C(=O)N[C@H](C(O)=O)CC(N)=O MLTRLIITQPXHBJ-BQBZGAKWSA-N 0.000 claims 1
- DLCOFDAHNMMQPP-SRVKXCTJSA-N Leu-Asp-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O DLCOFDAHNMMQPP-SRVKXCTJSA-N 0.000 claims 1
- WIDZHJTYKYBLSR-DCAQKATOSA-N Leu-Glu-Glu Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O WIDZHJTYKYBLSR-DCAQKATOSA-N 0.000 claims 1
- IWTBYNQNAPECCS-AVGNSLFASA-N Leu-Glu-His Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 IWTBYNQNAPECCS-AVGNSLFASA-N 0.000 claims 1
- PDQDCFBVYXEFSD-SRVKXCTJSA-N Leu-Leu-Asp Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O PDQDCFBVYXEFSD-SRVKXCTJSA-N 0.000 claims 1
- REPBGZHJKYWFMJ-KKUMJFAQSA-N Leu-Lys-His Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N REPBGZHJKYWFMJ-KKUMJFAQSA-N 0.000 claims 1
- ZDJQVSIPFLMNOX-RHYQMDGZSA-N Leu-Thr-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N ZDJQVSIPFLMNOX-RHYQMDGZSA-N 0.000 claims 1
- QESXLSQLQHHTIX-RHYQMDGZSA-N Leu-Val-Thr Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O QESXLSQLQHHTIX-RHYQMDGZSA-N 0.000 claims 1
- 101001090725 Leuconostoc gelidum Bacteriocin leucocin-A Proteins 0.000 claims 1
- KNKHAVVBVXKOGX-JXUBOQSCSA-N Lys-Ala-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O KNKHAVVBVXKOGX-JXUBOQSCSA-N 0.000 claims 1
- NCTDKZKNBDZDOL-GARJFASQSA-N Lys-Asn-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)N)NC(=O)[C@H](CCCCN)N)C(=O)O NCTDKZKNBDZDOL-GARJFASQSA-N 0.000 claims 1
- IGRMTQMIDNDFAA-UWVGGRQHSA-N Lys-His Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 IGRMTQMIDNDFAA-UWVGGRQHSA-N 0.000 claims 1
- OIQSIMFSVLLWBX-VOAKCMCISA-N Lys-Leu-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O OIQSIMFSVLLWBX-VOAKCMCISA-N 0.000 claims 1
- XOQMURBBIXRRCR-SRVKXCTJSA-N Lys-Lys-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCCCN XOQMURBBIXRRCR-SRVKXCTJSA-N 0.000 claims 1
- PLDJDCJLRCYPJB-VOAKCMCISA-N Lys-Lys-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PLDJDCJLRCYPJB-VOAKCMCISA-N 0.000 claims 1
- HYSVGEAWTGPMOA-IHRRRGAJSA-N Lys-Pro-Leu Chemical compound [H]N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(O)=O HYSVGEAWTGPMOA-IHRRRGAJSA-N 0.000 claims 1
- ZOKVLMBYDSIDKG-CSMHCCOUSA-N Lys-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H](N)CCCCN ZOKVLMBYDSIDKG-CSMHCCOUSA-N 0.000 claims 1
- VOAKKHOIAFKOQZ-JYJNAYRXSA-N Met-Tyr-Arg Chemical compound NC(=N)NCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CCSC)CC1=CC=C(O)C=C1 VOAKKHOIAFKOQZ-JYJNAYRXSA-N 0.000 claims 1
- SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 claims 1
- 101100205189 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) leu-5 gene Proteins 0.000 claims 1
- VADLTGVIOIOKGM-BZSNNMDCSA-N Phe-His-Leu Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(O)=O)NC(=O)[C@@H](N)CC=1C=CC=CC=1)C1=CN=CN1 VADLTGVIOIOKGM-BZSNNMDCSA-N 0.000 claims 1
- KLXQWABNAWDRAY-ACRUOGEOSA-N Phe-Lys-Phe Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 KLXQWABNAWDRAY-ACRUOGEOSA-N 0.000 claims 1
- RVQDZELMXZRSSI-IUCAKERBSA-N Pro-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1 RVQDZELMXZRSSI-IUCAKERBSA-N 0.000 claims 1
- DWGFLKQSGRUQTI-IHRRRGAJSA-N Pro-Lys-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H]1CCCN1 DWGFLKQSGRUQTI-IHRRRGAJSA-N 0.000 claims 1
- INCNPLPRPOYTJI-JBDRJPRFSA-N Ser-Cys-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CS)NC(=O)[C@H](CO)N INCNPLPRPOYTJI-JBDRJPRFSA-N 0.000 claims 1
- BXLYSRPHVMCOPS-ACZMJKKPSA-N Ser-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@@H](N)CO BXLYSRPHVMCOPS-ACZMJKKPSA-N 0.000 claims 1
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP83101035A EP0091539B2 (en) | 1982-03-31 | 1983-02-03 | Gene coding for interleukin-2 polypeptide, recombinant DNA carrying said gene, cell lines possessing the recombinant DNA,and method for producing interleukin-2 using said cells |
| GB83101035.0 | 1983-02-03 |
Related Child Applications (7)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24051583A Division JPS6034999A (ja) | 1983-12-20 | 1983-12-20 | ポリペプチド |
| JP59120492A Division JPS6034915A (ja) | 1983-02-03 | 1984-06-12 | ポリペプチド |
| JP1109061A Division JPH0659220B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドをコードする遺伝子 |
| JP1109060A Division JPH0659219B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドをコートする遺伝子 |
| JP1109058A Division JPH0832238B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドの製造法 |
| JP1109062A Division JPH0832239B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性を有するポリペプチドをコードする遺伝子を含有する組換えｄｎａ体および該組換えｄｎａ体により形質転換された原核生物細胞 |
| JP1109059A Division JPH0698000B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドをコードする遺伝子 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59144719A true JPS59144719A (ja) | 1984-08-18 |
| JPH0142279B2 JPH0142279B2 (enrdf_load_stackoverflow) | 1989-09-11 |
Family
ID=8190276
Family Applications (7)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58041358A Granted JPS59144719A (ja) | 1983-02-03 | 1983-03-12 | ヒトインタ―ロイキン２活性を有するポリペプチド |
| JP59120492A Pending JPS6034915A (ja) | 1983-02-03 | 1984-06-12 | ポリペプチド |
| JP1109058A Expired - Lifetime JPH0832238B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドの製造法 |
| JP1109061A Expired - Lifetime JPH0659220B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドをコードする遺伝子 |
| JP1109059A Expired - Lifetime JPH0698000B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドをコードする遺伝子 |
| JP1109060A Expired - Lifetime JPH0659219B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドをコートする遺伝子 |
| JP1109062A Expired - Lifetime JPH0832239B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性を有するポリペプチドをコードする遺伝子を含有する組換えｄｎａ体および該組換えｄｎａ体により形質転換された原核生物細胞 |
Family Applications After (6)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP59120492A Pending JPS6034915A (ja) | 1983-02-03 | 1984-06-12 | ポリペプチド |
| JP1109058A Expired - Lifetime JPH0832238B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドの製造法 |
| JP1109061A Expired - Lifetime JPH0659220B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドをコードする遺伝子 |
| JP1109059A Expired - Lifetime JPH0698000B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドをコードする遺伝子 |
| JP1109060A Expired - Lifetime JPH0659219B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性をもつポリペプチドをコートする遺伝子 |
| JP1109062A Expired - Lifetime JPH0832239B2 (ja) | 1983-02-03 | 1989-05-01 | ヒトインターロイキン２活性を有するポリペプチドをコードする遺伝子を含有する組換えｄｎａ体および該組換えｄｎａ体により形質転換された原核生物細胞 |
Country Status (2)
| Country | Link |
|---|---|
| JP (7) | JPS59144719A (enrdf_load_stackoverflow) |
| SU (1) | SU1479005A3 (enrdf_load_stackoverflow) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59220189A (ja) * | 1983-02-08 | 1984-12-11 | バイオジェン ナームローズ ベンノットシャップ | ひとインターロイキン２様ポリペプチドをコードするｄｎａ配列、該ｄｎａ配列を有する発現ベクターおよび該発現ベクターを有する大腸菌 |
| WO1986006405A1 (en) * | 1985-05-02 | 1986-11-06 | Takeda Chemical Industries, Ltd. | Novel transformant and its use |
| WO1986006410A1 (en) * | 1985-04-30 | 1986-11-06 | Takeda Chemical Industries, Ltd. | Process for increasing yield of interleukin-2 |
| JPS62185098A (ja) * | 1986-02-10 | 1987-08-13 | Otsuka Pharmaceut Co Ltd | インタ−ロイキン−２活性を有するポリペプチド |
| JPS6427497A (en) * | 1983-02-08 | 1989-01-30 | Biogen Nv | Human interleukin 2 like polypeptide |
| JPH07233082A (ja) * | 1983-12-23 | 1995-09-05 | F Hoffmann La Roche Ag | 免疫学的疾患治療用医薬組成物 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101041986B1 (ko) * | 2008-03-06 | 2011-06-16 | (주)한국비엠아이 | 인간 인터루킨-2의 대량 생산을 위한 신규한 균주 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6419879A (en) * | 1987-07-15 | 1989-01-23 | Asahi Optical Co Ltd | Tv viewer |
-
1983
- 1983-03-12 JP JP58041358A patent/JPS59144719A/ja active Granted
- 1983-12-14 SU SU833679351A patent/SU1479005A3/ru active
-
1984
- 1984-06-12 JP JP59120492A patent/JPS6034915A/ja active Pending
-
1989
- 1989-05-01 JP JP1109058A patent/JPH0832238B2/ja not_active Expired - Lifetime
- 1989-05-01 JP JP1109061A patent/JPH0659220B2/ja not_active Expired - Lifetime
- 1989-05-01 JP JP1109059A patent/JPH0698000B2/ja not_active Expired - Lifetime
- 1989-05-01 JP JP1109060A patent/JPH0659219B2/ja not_active Expired - Lifetime
- 1989-05-01 JP JP1109062A patent/JPH0832239B2/ja not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59220189A (ja) * | 1983-02-08 | 1984-12-11 | バイオジェン ナームローズ ベンノットシャップ | ひとインターロイキン２様ポリペプチドをコードするｄｎａ配列、該ｄｎａ配列を有する発現ベクターおよび該発現ベクターを有する大腸菌 |
| JPS6427497A (en) * | 1983-02-08 | 1989-01-30 | Biogen Nv | Human interleukin 2 like polypeptide |
| JPH07233082A (ja) * | 1983-12-23 | 1995-09-05 | F Hoffmann La Roche Ag | 免疫学的疾患治療用医薬組成物 |
| WO1986006410A1 (en) * | 1985-04-30 | 1986-11-06 | Takeda Chemical Industries, Ltd. | Process for increasing yield of interleukin-2 |
| WO1986006405A1 (en) * | 1985-05-02 | 1986-11-06 | Takeda Chemical Industries, Ltd. | Novel transformant and its use |
| JPS62185098A (ja) * | 1986-02-10 | 1987-08-13 | Otsuka Pharmaceut Co Ltd | インタ−ロイキン−２活性を有するポリペプチド |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0659220B2 (ja) | 1994-08-10 |
| JPH0659219B2 (ja) | 1994-08-10 |
| JPH0832239B2 (ja) | 1996-03-29 |
| JPH0335795A (ja) | 1991-02-15 |
| JPH02195887A (ja) | 1990-08-02 |
| JPH0698000B2 (ja) | 1994-12-07 |
| JPS6034915A (ja) | 1985-02-22 |
| JPH02200189A (ja) | 1990-08-08 |
| JPH0142279B2 (enrdf_load_stackoverflow) | 1989-09-11 |
| JPH02195888A (ja) | 1990-08-02 |
| SU1479005A3 (ru) | 1989-05-07 |
| JPH0832238B2 (ja) | 1996-03-29 |
| JPH02195886A (ja) | 1990-08-02 |
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