JPS5862116A - 肝細胞の増殖促進物質 - Google Patents
肝細胞の増殖促進物質Info
- Publication number
- JPS5862116A JPS5862116A JP56161679A JP16167981A JPS5862116A JP S5862116 A JPS5862116 A JP S5862116A JP 56161679 A JP56161679 A JP 56161679A JP 16167981 A JP16167981 A JP 16167981A JP S5862116 A JPS5862116 A JP S5862116A
- Authority
- JP
- Japan
- Prior art keywords
- cells
- liver
- small intestine
- dialyzed
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000003494 hepatocyte Anatomy 0.000 title claims description 20
- 230000035755 proliferation Effects 0.000 title description 7
- 239000000126 substance Substances 0.000 claims description 13
- 241001465754 Metazoa Species 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 9
- 210000004347 intestinal mucosa Anatomy 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 238000004587 chromatography analysis Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 241000700159 Rattus Species 0.000 description 31
- 210000004027 cell Anatomy 0.000 description 22
- 239000000284 extract Substances 0.000 description 20
- 210000000813 small intestine Anatomy 0.000 description 19
- 210000004185 liver Anatomy 0.000 description 15
- 239000000243 solution Substances 0.000 description 15
- 238000000034 method Methods 0.000 description 13
- 239000002609 medium Substances 0.000 description 9
- 238000001356 surgical procedure Methods 0.000 description 9
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 230000001172 regenerating effect Effects 0.000 description 8
- 241000287828 Gallus gallus Species 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 241000283707 Capra Species 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 210000004877 mucosa Anatomy 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 241000283973 Oryctolagus cuniculus Species 0.000 description 4
- 229910021529 ammonia Inorganic materials 0.000 description 4
- 210000005161 hepatic lobe Anatomy 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 3
- 210000001015 abdomen Anatomy 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000001962 electrophoresis Methods 0.000 description 3
- 238000010828 elution Methods 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 210000000936 intestine Anatomy 0.000 description 3
- 230000003908 liver function Effects 0.000 description 3
- 230000001737 promoting effect Effects 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 229920002125 Sokalan® Polymers 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 230000002440 hepatic effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 210000004738 parenchymal cell Anatomy 0.000 description 2
- 239000004584 polyacrylic acid Substances 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 108010062580 Concanavalin A Proteins 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 206010015719 Exsanguination Diseases 0.000 description 1
- -1 GMF Chemical compound 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 239000012981 Hank's balanced salt solution Substances 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 210000001715 carotid artery Anatomy 0.000 description 1
- 210000004534 cecum Anatomy 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000038 chest Anatomy 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- FSEUPUDHEBLWJY-HWKANZROSA-N diacetylmonoxime Chemical compound CC(=O)C(\C)=N\O FSEUPUDHEBLWJY-HWKANZROSA-N 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- VYQNWZOUAUKGHI-UHFFFAOYSA-N monobenzone Chemical compound C1=CC(O)=CC=C1OCC1=CC=CC=C1 VYQNWZOUAUKGHI-UHFFFAOYSA-N 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 238000012758 nuclear staining Methods 0.000 description 1
- 210000003200 peritoneal cavity Anatomy 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 229940070376 protein Drugs 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000000565 sealant Substances 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 229920000260 silastic Polymers 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 210000002417 xiphoid bone Anatomy 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56161679A JPS5862116A (ja) | 1981-10-09 | 1981-10-09 | 肝細胞の増殖促進物質 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56161679A JPS5862116A (ja) | 1981-10-09 | 1981-10-09 | 肝細胞の増殖促進物質 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5862116A true JPS5862116A (ja) | 1983-04-13 |
| JPH0148886B2 JPH0148886B2 (OSRAM) | 1989-10-20 |
Family
ID=15739776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56161679A Granted JPS5862116A (ja) | 1981-10-09 | 1981-10-09 | 肝細胞の増殖促進物質 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5862116A (OSRAM) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60243019A (ja) * | 1984-05-17 | 1985-12-03 | Mitsubishi Chem Ind Ltd | 肝細胞増殖因子 |
| US5547856A (en) * | 1992-05-18 | 1996-08-20 | Genentech, Inc. | Hepatocyte growth factor variants |
| US5580963A (en) * | 1992-05-18 | 1996-12-03 | Genentech, Inc. | Single-chain hepatocyte growth factor variants |
| US5879910A (en) * | 1992-05-18 | 1999-03-09 | Genetech, Inc. | Hepatocyte growth factor protease domain variants |
-
1981
- 1981-10-09 JP JP56161679A patent/JPS5862116A/ja active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60243019A (ja) * | 1984-05-17 | 1985-12-03 | Mitsubishi Chem Ind Ltd | 肝細胞増殖因子 |
| US5547856A (en) * | 1992-05-18 | 1996-08-20 | Genentech, Inc. | Hepatocyte growth factor variants |
| US5580963A (en) * | 1992-05-18 | 1996-12-03 | Genentech, Inc. | Single-chain hepatocyte growth factor variants |
| US5879910A (en) * | 1992-05-18 | 1999-03-09 | Genetech, Inc. | Hepatocyte growth factor protease domain variants |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0148886B2 (OSRAM) | 1989-10-20 |
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