JPH0148886B2 - - Google Patents
Info
- Publication number
- JPH0148886B2 JPH0148886B2 JP56161679A JP16167981A JPH0148886B2 JP H0148886 B2 JPH0148886 B2 JP H0148886B2 JP 56161679 A JP56161679 A JP 56161679A JP 16167981 A JP16167981 A JP 16167981A JP H0148886 B2 JPH0148886 B2 JP H0148886B2
- Authority
- JP
- Japan
- Prior art keywords
- small intestine
- extract
- solution
- cells
- proliferation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000003494 hepatocyte Anatomy 0.000 claims description 19
- 239000000126 substance Substances 0.000 claims description 10
- 241001465754 Metazoa Species 0.000 claims description 8
- 239000006228 supernatant Substances 0.000 claims description 8
- 238000004587 chromatography analysis Methods 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- 229920002684 Sepharose Polymers 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- 210000004347 intestinal mucosa Anatomy 0.000 claims description 5
- 238000000502 dialysis Methods 0.000 claims description 4
- 238000001962 electrophoresis Methods 0.000 claims description 4
- 229920002401 polyacrylamide Polymers 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 206010011224 Cough Diseases 0.000 claims description 2
- 230000000694 effects Effects 0.000 claims description 2
- 241000700159 Rattus Species 0.000 description 25
- 210000004027 cell Anatomy 0.000 description 21
- 239000000284 extract Substances 0.000 description 21
- 210000000813 small intestine Anatomy 0.000 description 20
- 239000000243 solution Substances 0.000 description 15
- 230000035755 proliferation Effects 0.000 description 12
- 239000002609 medium Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 8
- 241000287828 Gallus gallus Species 0.000 description 7
- 235000013330 chicken meat Nutrition 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 210000004185 liver Anatomy 0.000 description 6
- 241000283707 Capra Species 0.000 description 5
- 241000283973 Oryctolagus cuniculus Species 0.000 description 5
- 230000000968 intestinal effect Effects 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 230000003908 liver function Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 210000004877 mucosa Anatomy 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 238000001356 surgical procedure Methods 0.000 description 4
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000001172 regenerating effect Effects 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 238000000862 absorption spectrum Methods 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 210000001715 carotid artery Anatomy 0.000 description 2
- 210000004534 cecum Anatomy 0.000 description 2
- 230000021164 cell adhesion Effects 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000002440 hepatic effect Effects 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 210000004738 parenchymal cell Anatomy 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- -1 CMF Chemical compound 0.000 description 1
- 102000029816 Collagenase Human genes 0.000 description 1
- 108060005980 Collagenase Proteins 0.000 description 1
- 108010062580 Concanavalin A Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 206010015719 Exsanguination Diseases 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 239000012981 Hank's balanced salt solution Substances 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-N acetic acid Substances CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229960002424 collagenase Drugs 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- FSEUPUDHEBLWJY-HWKANZROSA-N diacetylmonoxime Chemical compound CC(=O)C(\C)=N\O FSEUPUDHEBLWJY-HWKANZROSA-N 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 238000011067 equilibration Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 210000001630 jejunum Anatomy 0.000 description 1
- 238000002350 laparotomy Methods 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56161679A JPS5862116A (ja) | 1981-10-09 | 1981-10-09 | 肝細胞の増殖促進物質 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP56161679A JPS5862116A (ja) | 1981-10-09 | 1981-10-09 | 肝細胞の増殖促進物質 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5862116A JPS5862116A (ja) | 1983-04-13 |
| JPH0148886B2 true JPH0148886B2 (OSRAM) | 1989-10-20 |
Family
ID=15739776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP56161679A Granted JPS5862116A (ja) | 1981-10-09 | 1981-10-09 | 肝細胞の増殖促進物質 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5862116A (OSRAM) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60243019A (ja) * | 1984-05-17 | 1985-12-03 | Mitsubishi Chem Ind Ltd | 肝細胞増殖因子 |
| DE69332221T2 (de) * | 1992-05-18 | 2003-04-17 | Genentech, Inc. | Hepatozytwachstumfaktor Variante |
| US5316921A (en) * | 1992-05-18 | 1994-05-31 | Genentech, Inc. | Single-chain hepatocyte growth factor variants |
| US5328837A (en) * | 1992-05-18 | 1994-07-12 | Genentech, Inc. | Hepatocyte growth factor protease domain variants |
-
1981
- 1981-10-09 JP JP56161679A patent/JPS5862116A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5862116A (ja) | 1983-04-13 |
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