JPS5824594A - 新規なプラスミド - Google Patents
新規なプラスミドInfo
- Publication number
- JPS5824594A JPS5824594A JP57124553A JP12455382A JPS5824594A JP S5824594 A JPS5824594 A JP S5824594A JP 57124553 A JP57124553 A JP 57124553A JP 12455382 A JP12455382 A JP 12455382A JP S5824594 A JPS5824594 A JP S5824594A
- Authority
- JP
- Japan
- Prior art keywords
- plasmid
- fragments
- cleaved
- dna
- lengths
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 44
- 239000013598 vector Substances 0.000 claims abstract description 12
- 238000010276 construction Methods 0.000 claims abstract 2
- 239000012634 fragment Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 11
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 claims 1
- 241000194017 Streptococcus Species 0.000 claims 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000010367 cloning Methods 0.000 abstract description 3
- 101100204460 Caenorhabditis elegans svh-1 gene Proteins 0.000 abstract 1
- 241000531819 Streptomyces venezuelae Species 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 19
- 241000187747 Streptomyces Species 0.000 description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 108091008146 restriction endonucleases Proteins 0.000 description 11
- 238000005119 centrifugation Methods 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 229940088598 enzyme Drugs 0.000 description 7
- 238000002955 isolation Methods 0.000 description 7
- 239000000725 suspension Substances 0.000 description 7
- 238000003776 cleavage reaction Methods 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 230000007017 scission Effects 0.000 description 6
- 241001655322 Streptomycetales Species 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- AIYUHDOJVYHVIT-UHFFFAOYSA-M caesium chloride Chemical compound [Cl-].[Cs+] AIYUHDOJVYHVIT-UHFFFAOYSA-M 0.000 description 5
- 239000013611 chromosomal DNA Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 210000001938 protoplast Anatomy 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 102000016943 Muramidase Human genes 0.000 description 3
- 108010014251 Muramidase Proteins 0.000 description 3
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 3
- 229960005542 ethidium bromide Drugs 0.000 description 3
- 229960000274 lysozyme Drugs 0.000 description 3
- 239000004325 lysozyme Substances 0.000 description 3
- 235000010335 lysozyme Nutrition 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 239000004471 Glycine Substances 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 230000009089 cytolysis Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 235000019833 protease Nutrition 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-NJFSPNSNSA-N Carbon-14 Chemical compound [14C] OKTJSMMVPCPJKN-NJFSPNSNSA-N 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- 229930182821 L-proline Natural products 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 241000187398 Streptomyces lividans Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000000432 density-gradient centrifugation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000002616 endonucleolytic effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 235000013882 gravy Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 229940113116 polyethylene glycol 1000 Drugs 0.000 description 1
- 229940093429 polyethylene glycol 6000 Drugs 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 229960002429 proline Drugs 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007363 ring formation reaction Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229940104230 thymidine Drugs 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
- Y10S435/906—Streptomyces venezuelae
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3128669.0 | 1981-07-20 | ||
| DE19813128669 DE3128669A1 (de) | 1981-07-20 | 1981-07-20 | "plasmid p svh 1 und seine verwendung" |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5824594A true JPS5824594A (ja) | 1983-02-14 |
| JPH0363357B2 JPH0363357B2 (cg-RX-API-DMAC7.html) | 1991-09-30 |
Family
ID=6137332
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57124553A Granted JPS5824594A (ja) | 1981-07-20 | 1982-07-19 | 新規なプラスミド |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US4673642A (cg-RX-API-DMAC7.html) |
| EP (1) | EP0070522B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JPS5824594A (cg-RX-API-DMAC7.html) |
| AT (1) | ATE23190T1 (cg-RX-API-DMAC7.html) |
| AU (1) | AU550419B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA1187824A (cg-RX-API-DMAC7.html) |
| DE (2) | DE3128669A1 (cg-RX-API-DMAC7.html) |
| DK (1) | DK160999C (cg-RX-API-DMAC7.html) |
| ES (1) | ES8306176A1 (cg-RX-API-DMAC7.html) |
| IL (1) | IL66344A (cg-RX-API-DMAC7.html) |
| NO (1) | NO822490L (cg-RX-API-DMAC7.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60217895A (ja) * | 1984-03-31 | 1985-10-31 | ヘキスト・アクチエンゲゼルシヤフト | ストレプトミセス科レプリコンおよび大腸菌レプリコンを有するハイブリツドプラスミド |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA832526B (en) * | 1982-04-16 | 1984-11-28 | Lilly Co Eli | Chimeric cloning vectors for use in streptomyces and e.coli |
| CA1203185A (en) * | 1982-06-03 | 1986-04-15 | Thomas G. Eckhardt | Cloned streptomycete gene |
| DE3477936D1 (en) * | 1983-03-08 | 1989-06-01 | Merck & Co Inc | Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes |
| US4703009A (en) * | 1983-03-08 | 1987-10-27 | Merck & Co., Inc. | RDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes |
| AU594161B2 (en) * | 1986-06-24 | 1990-03-01 | Enterovax Limited | Non-antibiotic marker system |
| KR960015610B1 (ko) * | 1987-06-22 | 1996-11-18 | 제네틱스 인스티튜트, 인코포레이티드 | 신규의 혈전붕괴 단백질의 제조방법 |
| BR0012627A (pt) * | 1999-07-23 | 2002-04-09 | Genentech Inc | Processo para purificar o dna de plasmìdeo de células procarióticas mediante o emprego de filtração de fluxo tangencial |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1979001169A1 (en) * | 1978-06-01 | 1979-12-27 | D Hopwood | Streptomyces plasmids and their use as vectors for the incorporation of nucleic acid into cellular systems |
| US4273875A (en) * | 1979-03-05 | 1981-06-16 | The Upjohn Company | Plasmid and process of isolating same |
| US4343906A (en) * | 1979-08-21 | 1982-08-10 | The Upjohn Company | Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli containing same |
| US4340674A (en) * | 1980-05-05 | 1982-07-20 | The Upjohn Company | Cointegrate plasmids and their construction from plasmids of Escherichia and Streptomyces |
| US4332900A (en) * | 1980-10-01 | 1982-06-01 | The Upjohn Company | Construction of co-integrate plasmids from plasmids of Streptomyces and Escherichia |
| US4401761A (en) * | 1981-01-26 | 1983-08-30 | The Upjohn Company | Process for stabilizing plasmids by deletion of DNA |
| EP0058002A3 (en) * | 1981-01-26 | 1983-06-29 | The Upjohn Company | Process for stabilising potential plasmid vectors and novel plasmids |
-
1981
- 1981-07-20 DE DE19813128669 patent/DE3128669A1/de not_active Withdrawn
-
1982
- 1982-07-14 ES ES513947A patent/ES8306176A1/es not_active Expired
- 1982-07-15 DE DE8282106361T patent/DE3273995D1/de not_active Expired
- 1982-07-15 EP EP82106361A patent/EP0070522B1/de not_active Expired
- 1982-07-15 AT AT82106361T patent/ATE23190T1/de active
- 1982-07-16 CA CA000407435A patent/CA1187824A/en not_active Expired
- 1982-07-19 DK DK324082A patent/DK160999C/da active
- 1982-07-19 IL IL66344A patent/IL66344A/xx unknown
- 1982-07-19 AU AU86173/82A patent/AU550419B2/en not_active Ceased
- 1982-07-19 NO NO822490A patent/NO822490L/no unknown
- 1982-07-19 JP JP57124553A patent/JPS5824594A/ja active Granted
-
1984
- 1984-08-20 US US06/642,742 patent/US4673642A/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60217895A (ja) * | 1984-03-31 | 1985-10-31 | ヘキスト・アクチエンゲゼルシヤフト | ストレプトミセス科レプリコンおよび大腸菌レプリコンを有するハイブリツドプラスミド |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3273995D1 (en) | 1986-12-04 |
| DK160999C (da) | 1991-11-18 |
| EP0070522A2 (de) | 1983-01-26 |
| IL66344A0 (en) | 1982-11-30 |
| AU8617382A (en) | 1983-01-27 |
| EP0070522B1 (de) | 1986-10-29 |
| DE3128669A1 (de) | 1983-02-03 |
| DK160999B (da) | 1991-05-13 |
| ES513947A0 (es) | 1983-05-16 |
| NO822490L (no) | 1983-01-21 |
| IL66344A (en) | 1985-08-30 |
| ATE23190T1 (de) | 1986-11-15 |
| ES8306176A1 (es) | 1983-05-16 |
| AU550419B2 (en) | 1986-03-20 |
| JPH0363357B2 (cg-RX-API-DMAC7.html) | 1991-09-30 |
| DK324082A (da) | 1983-01-21 |
| US4673642A (en) | 1987-06-16 |
| EP0070522A3 (en) | 1983-06-22 |
| CA1187824A (en) | 1985-05-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Niaudet et al. | In vitro genetic labeling of Bacillus subtilis cryptic plasmid pHV400 | |
| US4359535A (en) | Autonomously replicating DNA containing inserted DNA sequences | |
| Crabeel et al. | Cloning and endonuclease restriction analysis of argF and of the control region of the argECBH bipolar operon in Escherichia coli | |
| US4528266A (en) | Method of inserting unique DNA sequences into DNA vectors | |
| Liu et al. | CAPTURE: in situ analysis of chromatin composition of endogenous genomic loci by biotinylated dCas9 | |
| JPS5824594A (ja) | 新規なプラスミド | |
| Huang et al. | The telomere system of the Streptomyces linear plasmid SCP1 represents a novel class | |
| CN112063643A (zh) | 一种用于检测细菌中膜蛋白相互作用的表达载体及方法 | |
| CN116286931B (zh) | 用于富养罗尔斯通氏菌快速基因编辑的双质粒系统及应用 | |
| CN103103180B (zh) | 一种从土壤中分离纯化大片段dna的方法 | |
| Gong et al. | Modification of bacterial artificial chromosomes (BACs) and preparation of intact BAC DNA for generation of transgenic mice | |
| Babu et al. | Identification of protein complexes in Escherichia coli using sequential peptide affinity purification in combination with tandem mass spectrometry | |
| Lo Sciuto et al. | Generation of stable and unmarked conditional mutants in Pseudomonas aeruginosa | |
| Löfdahl et al. | A vector for recombinant DNA in Staphylococcus aureus | |
| EP0438232A2 (en) | Dna sequence conferring a plaque inhibition phenotype | |
| US20240376454A1 (en) | Type i-a crispr-cas3 system for genome editing and diagnostics | |
| Finnegan et al. | Physical map of the coliphage 186 chromosome: I. Gene content of the Bam HI, Pst I and other restriction fragments | |
| Hillebrand et al. | A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi | |
| MacNeil | A flexible boiling procedure for isolating plasmid DNA from gram-positive microorganisms | |
| CN108707590B (zh) | 一种Pictet-Spengler酶及其编码基因与应用 | |
| CN108220257A (zh) | Phd2蛋白的体外表达与纯化方法 | |
| LU509362B1 (en) | Gateway system vector kits for rapid and efficient expression of plant active proteins | |
| Glover | The principles of cloning DNA | |
| JPS58189199A (ja) | 枯草菌用のプラスミド・クロ−ニング・ベクタ− | |
| EP0475195A2 (en) | Cloning of Sac II restriction endonuclease and methylase |