IL68349A0 - Chimeric cloning vectors for use in streptomyces and e.coli - Google Patents
Chimeric cloning vectors for use in streptomyces and e.coliInfo
- Publication number
- IL68349A0 IL68349A0 IL68349A IL6834983A IL68349A0 IL 68349 A0 IL68349 A0 IL 68349A0 IL 68349 A IL68349 A IL 68349A IL 6834983 A IL6834983 A IL 6834983A IL 68349 A0 IL68349 A0 IL 68349A0
- Authority
- IL
- Israel
- Prior art keywords
- streptomyces
- coli
- antibiotic
- cell
- resistance
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Abstract
There is described a recombinant DNA cloning vector useful in Streptomyces and E. coli, comprising a) a functional origin of replication- containing restriction fragment of plasmid SCP2 or SCP2*, b) a restriction fragment comprising an E. coli origin of replication, c) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a cell of E. coli, said cell being sensitive to the antibiotic for which resistance is conferred, and d) one or more DNA segments that independently confer either or both of the Streptomyces tra function or resistance to at least one antibiotic when transformed into a cell of Streptomyces, said cell being sensitive to the antibiotic for which resistance is conferred. Transformants and a method for detecting transformants of the vector are also disclosed.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36894782A | 1982-04-16 | 1982-04-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL68349A0 true IL68349A0 (en) | 1983-07-31 |
Family
ID=23453416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL68349A IL68349A0 (en) | 1982-04-16 | 1983-04-11 | Chimeric cloning vectors for use in streptomyces and e.coli |
Country Status (19)
| Country | Link |
|---|---|
| JP (1) | JPS58189198A (en) |
| KR (1) | KR840004451A (en) |
| AT (1) | ATE54327T1 (en) |
| AU (1) | AU570631B2 (en) |
| CA (1) | CA1207685A (en) |
| DD (1) | DD211360A5 (en) |
| DE (1) | DE3381699D1 (en) |
| DK (1) | DK162083A (en) |
| ES (1) | ES521468A0 (en) |
| FI (1) | FI831245L (en) |
| GB (1) | GB2118947B (en) |
| GR (1) | GR78559B (en) |
| IE (1) | IE54818B1 (en) |
| IL (1) | IL68349A0 (en) |
| NZ (1) | NZ203864A (en) |
| PH (1) | PH21843A (en) |
| PL (1) | PL241511A1 (en) |
| PT (1) | PT76536B (en) |
| ZA (1) | ZA832526B (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1203185A (en) * | 1982-06-03 | 1986-04-15 | Thomas G. Eckhardt | Cloned streptomycete gene |
| US4468462A (en) * | 1982-12-22 | 1984-08-28 | Eli Lilly And Company | Vectors for cloning in streptomyces |
| US4753886A (en) * | 1984-08-10 | 1988-06-28 | Eli Lilly And Company | Plasmid PHJL210 and related bifunctional cloning vectors for use in streptomycetes |
| IL76421A0 (en) * | 1984-09-27 | 1986-01-31 | Lilly Co Eli | Improvements in or relating to recombinant dna cosmid shuttle vectors |
| US5149639A (en) * | 1986-03-24 | 1992-09-22 | Abbott Laboratories | Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production |
| US4874748A (en) * | 1986-03-24 | 1989-10-17 | Abbott Laboratories | Cloning vectors for streptomyces and use thereof in macrolide antibiotic production |
| JP4726719B2 (en) * | 2006-03-31 | 2011-07-20 | 花王株式会社 | Paper sheet manufacturing method and paper machine |
| LT2705157T (en) * | 2011-05-06 | 2017-03-10 | New England Biolabs, Inc. | Ligation enhancement |
| CN114214356B (en) * | 2021-12-28 | 2023-11-24 | 塔里木大学 | Construction method of Streptomyces roseoflavus TRM49605 genetic operating system |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3117131A1 (en) * | 1981-04-30 | 1982-11-25 | Hoechst Ag, 6000 Frankfurt | "PLASMID PSG 2 AND METHOD FOR ITS PRODUCTION" |
| DE3128669A1 (en) * | 1981-07-20 | 1983-02-03 | Hoechst Ag, 6000 Frankfurt | "PLASMID P SVH 1 AND ITS USE" |
| US4503155A (en) * | 1982-02-01 | 1985-03-05 | Eli Lilly And Company | Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and E. coli |
-
1983
- 1983-04-11 IL IL68349A patent/IL68349A0/en not_active IP Right Cessation
- 1983-04-11 ZA ZA832526A patent/ZA832526B/en unknown
- 1983-04-12 NZ NZ203864A patent/NZ203864A/en unknown
- 1983-04-12 PT PT76536A patent/PT76536B/en unknown
- 1983-04-12 CA CA000425651A patent/CA1207685A/en not_active Expired
- 1983-04-12 PH PH28761A patent/PH21843A/en unknown
- 1983-04-13 AU AU13476/83A patent/AU570631B2/en not_active Ceased
- 1983-04-13 FI FI831245A patent/FI831245L/en not_active Application Discontinuation
- 1983-04-13 DK DK162083A patent/DK162083A/en not_active IP Right Cessation
- 1983-04-14 ES ES521468A patent/ES521468A0/en active Granted
- 1983-04-14 KR KR1019830001563A patent/KR840004451A/en not_active Application Discontinuation
- 1983-04-14 AT AT83302116T patent/ATE54327T1/en not_active IP Right Cessation
- 1983-04-14 GB GB08310069A patent/GB2118947B/en not_active Expired
- 1983-04-14 IE IE848/83A patent/IE54818B1/en unknown
- 1983-04-14 DE DE8383302116T patent/DE3381699D1/en not_active Expired - Lifetime
- 1983-04-14 GR GR71081A patent/GR78559B/el unknown
- 1983-04-15 JP JP58067669A patent/JPS58189198A/en active Pending
- 1983-04-15 PL PL24151183A patent/PL241511A1/en unknown
- 1983-04-15 DD DD83249906A patent/DD211360A5/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DK162083D0 (en) | 1983-04-13 |
| AU570631B2 (en) | 1988-03-24 |
| PH21843A (en) | 1988-03-17 |
| PL241511A1 (en) | 1983-12-05 |
| GR78559B (en) | 1984-09-27 |
| KR840004451A (en) | 1984-10-15 |
| IE830848L (en) | 1983-10-16 |
| AU1347683A (en) | 1983-10-20 |
| JPS58189198A (en) | 1983-11-04 |
| IE54818B1 (en) | 1990-02-14 |
| ATE54327T1 (en) | 1990-07-15 |
| GB2118947B (en) | 1985-07-03 |
| FI831245A0 (en) | 1983-04-13 |
| ES8500995A1 (en) | 1984-11-01 |
| ES521468A0 (en) | 1984-11-01 |
| CA1207685A (en) | 1986-07-15 |
| GB2118947A (en) | 1983-11-09 |
| ZA832526B (en) | 1984-11-28 |
| FI831245L (en) | 1983-10-17 |
| DD211360A5 (en) | 1984-07-11 |
| PT76536B (en) | 1986-03-12 |
| DE3381699D1 (en) | 1990-08-09 |
| NZ203864A (en) | 1986-08-08 |
| GB8310069D0 (en) | 1983-05-18 |
| PT76536A (en) | 1983-05-01 |
| DK162083A (en) | 1983-10-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RH | Patent void |