IL68349A0 - Chimeric cloning vectors for use in streptomyces and e.coli - Google Patents

Chimeric cloning vectors for use in streptomyces and e.coli

Info

Publication number
IL68349A0
IL68349A0 IL68349A IL6834983A IL68349A0 IL 68349 A0 IL68349 A0 IL 68349A0 IL 68349 A IL68349 A IL 68349A IL 6834983 A IL6834983 A IL 6834983A IL 68349 A0 IL68349 A0 IL 68349A0
Authority
IL
Israel
Prior art keywords
streptomyces
coli
antibiotic
cell
resistance
Prior art date
Application number
IL68349A
Original Assignee
Lilly Co Eli
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lilly Co Eli filed Critical Lilly Co Eli
Publication of IL68349A0 publication Critical patent/IL68349A0/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Saccharide Compounds (AREA)
  • Portable Nailing Machines And Staplers (AREA)

Abstract

There is described a recombinant DNA cloning vector useful in Streptomyces and E. coli, comprising a) a functional origin of replication- containing restriction fragment of plasmid SCP2 or SCP2*, b) a restriction fragment comprising an E. coli origin of replication, c) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a cell of E. coli, said cell being sensitive to the antibiotic for which resistance is conferred, and d) one or more DNA segments that independently confer either or both of the Streptomyces tra function or resistance to at least one antibiotic when transformed into a cell of Streptomyces, said cell being sensitive to the antibiotic for which resistance is conferred. Transformants and a method for detecting transformants of the vector are also disclosed.
IL68349A 1982-04-16 1983-04-11 Chimeric cloning vectors for use in streptomyces and e.coli IL68349A0 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36894782A 1982-04-16 1982-04-16

Publications (1)

Publication Number Publication Date
IL68349A0 true IL68349A0 (en) 1983-07-31

Family

ID=23453416

Family Applications (1)

Application Number Title Priority Date Filing Date
IL68349A IL68349A0 (en) 1982-04-16 1983-04-11 Chimeric cloning vectors for use in streptomyces and e.coli

Country Status (19)

Country Link
JP (1) JPS58189198A (en)
KR (1) KR840004451A (en)
AT (1) ATE54327T1 (en)
AU (1) AU570631B2 (en)
CA (1) CA1207685A (en)
DD (1) DD211360A5 (en)
DE (1) DE3381699D1 (en)
DK (1) DK162083A (en)
ES (1) ES8500995A1 (en)
FI (1) FI831245L (en)
GB (1) GB2118947B (en)
GR (1) GR78559B (en)
IE (1) IE54818B1 (en)
IL (1) IL68349A0 (en)
NZ (1) NZ203864A (en)
PH (1) PH21843A (en)
PL (1) PL241511A1 (en)
PT (1) PT76536B (en)
ZA (1) ZA832526B (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1203185A (en) * 1982-06-03 1986-04-15 Thomas G. Eckhardt Cloned streptomycete gene
US4468462A (en) * 1982-12-22 1984-08-28 Eli Lilly And Company Vectors for cloning in streptomyces
US4753886A (en) * 1984-08-10 1988-06-28 Eli Lilly And Company Plasmid PHJL210 and related bifunctional cloning vectors for use in streptomycetes
IL76421A0 (en) * 1984-09-27 1986-01-31 Lilly Co Eli Improvements in or relating to recombinant dna cosmid shuttle vectors
US4874748A (en) * 1986-03-24 1989-10-17 Abbott Laboratories Cloning vectors for streptomyces and use thereof in macrolide antibiotic production
US5149639A (en) * 1986-03-24 1992-09-22 Abbott Laboratories Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production
JP4726719B2 (en) * 2006-03-31 2011-07-20 花王株式会社 Paper sheet manufacturing method and paper machine
WO2013022504A1 (en) * 2011-05-06 2013-02-14 New England Biolabs, Inc. Ligation enhancement
CN114214356B (en) * 2021-12-28 2023-11-24 塔里木大学 Construction method of Streptomyces roseoflavus TRM49605 genetic operating system

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3117131A1 (en) * 1981-04-30 1982-11-25 Hoechst Ag, 6000 Frankfurt "PLASMID PSG 2 AND METHOD FOR ITS PRODUCTION"
DE3128669A1 (en) * 1981-07-20 1983-02-03 Hoechst Ag, 6000 Frankfurt "PLASMID P SVH 1 AND ITS USE"
US4503155A (en) * 1982-02-01 1985-03-05 Eli Lilly And Company Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and E. coli

Also Published As

Publication number Publication date
IE830848L (en) 1983-10-16
AU570631B2 (en) 1988-03-24
DK162083D0 (en) 1983-04-13
PL241511A1 (en) 1983-12-05
DK162083A (en) 1983-10-17
CA1207685A (en) 1986-07-15
IE54818B1 (en) 1990-02-14
FI831245A0 (en) 1983-04-13
PH21843A (en) 1988-03-17
GB2118947B (en) 1985-07-03
GB8310069D0 (en) 1983-05-18
ATE54327T1 (en) 1990-07-15
DD211360A5 (en) 1984-07-11
DE3381699D1 (en) 1990-08-09
PT76536B (en) 1986-03-12
ZA832526B (en) 1984-11-28
KR840004451A (en) 1984-10-15
GB2118947A (en) 1983-11-09
GR78559B (en) 1984-09-27
ES521468A0 (en) 1984-11-01
ES8500995A1 (en) 1984-11-01
JPS58189198A (en) 1983-11-04
AU1347683A (en) 1983-10-20
FI831245L (en) 1983-10-17
NZ203864A (en) 1986-08-08
PT76536A (en) 1983-05-01

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