PT76536A - Process for preparing chimerics cloning vectors for use in streptomyces and e.coli - Google Patents
Process for preparing chimerics cloning vectors for use in streptomyces and e.coliInfo
- Publication number
- PT76536A PT76536A PT76536A PT7653683A PT76536A PT 76536 A PT76536 A PT 76536A PT 76536 A PT76536 A PT 76536A PT 7653683 A PT7653683 A PT 7653683A PT 76536 A PT76536 A PT 76536A
- Authority
- PT
- Portugal
- Prior art keywords
- streptomyces
- coli
- antibiotic
- cell
- resistance
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Portable Nailing Machines And Staplers (AREA)
- Saccharide Compounds (AREA)
Abstract
There is described a recombinant DNA cloning vector useful in Streptomyces and E. coli, comprising a) a functional origin of replication- containing restriction fragment of plasmid SCP2 or SCP2*, b) a restriction fragment comprising an E. coli origin of replication, c) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a cell of E. coli, said cell being sensitive to the antibiotic for which resistance is conferred, and d) one or more DNA segments that independently confer either or both of the Streptomyces tra function or resistance to at least one antibiotic when transformed into a cell of Streptomyces, said cell being sensitive to the antibiotic for which resistance is conferred. Transformants and a method for detecting transformants of the vector are also disclosed.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36894782A | 1982-04-16 | 1982-04-16 |
Publications (2)
Publication Number | Publication Date |
---|---|
PT76536A true PT76536A (en) | 1983-05-01 |
PT76536B PT76536B (en) | 1986-03-12 |
Family
ID=23453416
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PT76536A PT76536B (en) | 1982-04-16 | 1983-04-12 | Process for preparing chimerics cloning vectors for use in streptomyces and e.coli |
Country Status (19)
Country | Link |
---|---|
JP (1) | JPS58189198A (en) |
KR (1) | KR840004451A (en) |
AT (1) | ATE54327T1 (en) |
AU (1) | AU570631B2 (en) |
CA (1) | CA1207685A (en) |
DD (1) | DD211360A5 (en) |
DE (1) | DE3381699D1 (en) |
DK (1) | DK162083A (en) |
ES (1) | ES521468A0 (en) |
FI (1) | FI831245L (en) |
GB (1) | GB2118947B (en) |
GR (1) | GR78559B (en) |
IE (1) | IE54818B1 (en) |
IL (1) | IL68349A0 (en) |
NZ (1) | NZ203864A (en) |
PH (1) | PH21843A (en) |
PL (1) | PL241511A1 (en) |
PT (1) | PT76536B (en) |
ZA (1) | ZA832526B (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1203185A (en) * | 1982-06-03 | 1986-04-15 | Thomas G. Eckhardt | Cloned streptomycete gene |
US4468462A (en) * | 1982-12-22 | 1984-08-28 | Eli Lilly And Company | Vectors for cloning in streptomyces |
US4753886A (en) * | 1984-08-10 | 1988-06-28 | Eli Lilly And Company | Plasmid PHJL210 and related bifunctional cloning vectors for use in streptomycetes |
IL76421A0 (en) * | 1984-09-27 | 1986-01-31 | Lilly Co Eli | Improvements in or relating to recombinant dna cosmid shuttle vectors |
US4874748A (en) * | 1986-03-24 | 1989-10-17 | Abbott Laboratories | Cloning vectors for streptomyces and use thereof in macrolide antibiotic production |
US5149639A (en) * | 1986-03-24 | 1992-09-22 | Abbott Laboratories | Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production |
JP4726719B2 (en) * | 2006-03-31 | 2011-07-20 | 花王株式会社 | Paper sheet manufacturing method and paper machine |
WO2013022504A1 (en) * | 2011-05-06 | 2013-02-14 | New England Biolabs, Inc. | Ligation enhancement |
CN114214356B (en) * | 2021-12-28 | 2023-11-24 | 塔里木大学 | Construction method of Streptomyces roseoflavus TRM49605 genetic operating system |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3117131A1 (en) * | 1981-04-30 | 1982-11-25 | Hoechst Ag, 6000 Frankfurt | "PLASMID PSG 2 AND METHOD FOR ITS PRODUCTION" |
DE3128669A1 (en) * | 1981-07-20 | 1983-02-03 | Hoechst Ag, 6000 Frankfurt | "PLASMID P SVH 1 AND ITS USE" |
US4503155A (en) * | 1982-02-01 | 1985-03-05 | Eli Lilly And Company | Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and E. coli |
-
1983
- 1983-04-11 IL IL68349A patent/IL68349A0/en not_active IP Right Cessation
- 1983-04-11 ZA ZA832526A patent/ZA832526B/en unknown
- 1983-04-12 NZ NZ203864A patent/NZ203864A/en unknown
- 1983-04-12 PH PH28761A patent/PH21843A/en unknown
- 1983-04-12 PT PT76536A patent/PT76536B/en unknown
- 1983-04-12 CA CA000425651A patent/CA1207685A/en not_active Expired
- 1983-04-13 AU AU13476/83A patent/AU570631B2/en not_active Ceased
- 1983-04-13 FI FI831245A patent/FI831245L/en not_active Application Discontinuation
- 1983-04-13 DK DK162083A patent/DK162083A/en not_active IP Right Cessation
- 1983-04-14 KR KR1019830001563A patent/KR840004451A/en not_active Application Discontinuation
- 1983-04-14 ES ES521468A patent/ES521468A0/en active Granted
- 1983-04-14 GB GB08310069A patent/GB2118947B/en not_active Expired
- 1983-04-14 DE DE8383302116T patent/DE3381699D1/en not_active Expired - Lifetime
- 1983-04-14 GR GR71081A patent/GR78559B/el unknown
- 1983-04-14 IE IE848/83A patent/IE54818B1/en unknown
- 1983-04-14 AT AT83302116T patent/ATE54327T1/en not_active IP Right Cessation
- 1983-04-15 PL PL24151183A patent/PL241511A1/en unknown
- 1983-04-15 DD DD83249906A patent/DD211360A5/en unknown
- 1983-04-15 JP JP58067669A patent/JPS58189198A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
JPS58189198A (en) | 1983-11-04 |
GB2118947A (en) | 1983-11-09 |
DK162083A (en) | 1983-10-17 |
CA1207685A (en) | 1986-07-15 |
ES8500995A1 (en) | 1984-11-01 |
PH21843A (en) | 1988-03-17 |
PT76536B (en) | 1986-03-12 |
FI831245A0 (en) | 1983-04-13 |
DD211360A5 (en) | 1984-07-11 |
AU570631B2 (en) | 1988-03-24 |
DE3381699D1 (en) | 1990-08-09 |
GB2118947B (en) | 1985-07-03 |
KR840004451A (en) | 1984-10-15 |
ATE54327T1 (en) | 1990-07-15 |
NZ203864A (en) | 1986-08-08 |
ZA832526B (en) | 1984-11-28 |
FI831245L (en) | 1983-10-17 |
PL241511A1 (en) | 1983-12-05 |
AU1347683A (en) | 1983-10-20 |
IL68349A0 (en) | 1983-07-31 |
DK162083D0 (en) | 1983-04-13 |
IE830848L (en) | 1983-10-16 |
ES521468A0 (en) | 1984-11-01 |
IE54818B1 (en) | 1990-02-14 |
GR78559B (en) | 1984-09-27 |
GB8310069D0 (en) | 1983-05-18 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
IE812297L (en) | Stabilising transformed cells | |
EP0077650A3 (en) | Cloning vectors | |
ZA822855B (en) | Bacillus cloning vectors,recombinant dna molecules,bacillus hosts transformed with them and methods for expressing foreign dna sequences and producing polypeptides coded thereby | |
GB8424454D0 (en) | Recombinant dna cloning vectors | |
EP0077649A3 (en) | Cloning vectors for use in Streptomyces | |
PT76536B (en) | Process for preparing chimerics cloning vectors for use in streptomyces and e.coli | |
WO1995002058A3 (en) | Bacillus thuringiensis transposon tn5401 and its use in a site-specific recombination system for bacillus thuringiensis strain development | |
EP0215625A3 (en) | Protein recovery | |
EP0115173A3 (en) | Cloning vectors in streptomyces | |
DE3477936D1 (en) | Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes | |
DK460183A (en) | FUNCTIONAL INDEPENDENT RECOMBINANT DNA CLONING VECTORS FOR USE IN STREPTOMYCES | |
DK360785D0 (en) | PLASMID PHJL210 AND THEREOF RELATED BIFUNCTIONAL CLONING VECTOR FOR USE IN STREPTOMYCES | |
DK0533701T3 (en) | Ferry vector for developing recombinant Bacillus thuringiensis strain | |
DK198188D0 (en) | RECOMBINANT DNA CLONING VECTORS EFFECTING SPIRAMYCINE RESISTANCE | |
Cohen et al. | RNA polymerase from vegetative cells and spores of Bacillus cereus T: II. Peptide composition | |
SG74694G (en) | Recombinant immunoglobulin preparations, methods for their preparation, DNA sequences, expression vectors and recombinant host cells therefor |