ZA832526B - Chimeric cloning vectors for use in streptomyces and e.coli - Google Patents
Chimeric cloning vectors for use in streptomyces and e.coliInfo
- Publication number
- ZA832526B ZA832526B ZA832526A ZA832526A ZA832526B ZA 832526 B ZA832526 B ZA 832526B ZA 832526 A ZA832526 A ZA 832526A ZA 832526 A ZA832526 A ZA 832526A ZA 832526 B ZA832526 B ZA 832526B
- Authority
- ZA
- South Africa
- Prior art keywords
- streptomyces
- coli
- antibiotic
- cell
- resistance
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Saccharide Compounds (AREA)
- Portable Nailing Machines And Staplers (AREA)
Abstract
There is described a recombinant DNA cloning vector useful in Streptomyces and E. coli, comprising a) a functional origin of replication- containing restriction fragment of plasmid SCP2 or SCP2*, b) a restriction fragment comprising an E. coli origin of replication, c) one or more DNA segments that confer resistance to at least one antibiotic when transformed into a cell of E. coli, said cell being sensitive to the antibiotic for which resistance is conferred, and d) one or more DNA segments that independently confer either or both of the Streptomyces tra function or resistance to at least one antibiotic when transformed into a cell of Streptomyces, said cell being sensitive to the antibiotic for which resistance is conferred. Transformants and a method for detecting transformants of the vector are also disclosed.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36894782A | 1982-04-16 | 1982-04-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ZA832526B true ZA832526B (en) | 1984-11-28 |
Family
ID=23453416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ZA832526A ZA832526B (en) | 1982-04-16 | 1983-04-11 | Chimeric cloning vectors for use in streptomyces and e.coli |
Country Status (19)
| Country | Link |
|---|---|
| JP (1) | JPS58189198A (en) |
| KR (1) | KR840004451A (en) |
| AT (1) | ATE54327T1 (en) |
| AU (1) | AU570631B2 (en) |
| CA (1) | CA1207685A (en) |
| DD (1) | DD211360A5 (en) |
| DE (1) | DE3381699D1 (en) |
| DK (1) | DK162083A (en) |
| ES (1) | ES8500995A1 (en) |
| FI (1) | FI831245L (en) |
| GB (1) | GB2118947B (en) |
| GR (1) | GR78559B (en) |
| IE (1) | IE54818B1 (en) |
| IL (1) | IL68349A0 (en) |
| NZ (1) | NZ203864A (en) |
| PH (1) | PH21843A (en) |
| PL (1) | PL241511A1 (en) |
| PT (1) | PT76536B (en) |
| ZA (1) | ZA832526B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114214356A (en) * | 2021-12-28 | 2022-03-22 | 塔里木大学 | Construction method of genetic operation system of rochondrium sp TRM49605 |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1203185A (en) * | 1982-06-03 | 1986-04-15 | Thomas G. Eckhardt | Cloned streptomycete gene |
| US4468462A (en) * | 1982-12-22 | 1984-08-28 | Eli Lilly And Company | Vectors for cloning in streptomyces |
| US4753886A (en) * | 1984-08-10 | 1988-06-28 | Eli Lilly And Company | Plasmid PHJL210 and related bifunctional cloning vectors for use in streptomycetes |
| IL76421A0 (en) * | 1984-09-27 | 1986-01-31 | Lilly Co Eli | Improvements in or relating to recombinant dna cosmid shuttle vectors |
| US4874748A (en) * | 1986-03-24 | 1989-10-17 | Abbott Laboratories | Cloning vectors for streptomyces and use thereof in macrolide antibiotic production |
| US5149639A (en) * | 1986-03-24 | 1992-09-22 | Abbott Laboratories | Biologically pure cultures of streptomyces and use thereof in macrolide antibiotic production |
| JP4726719B2 (en) * | 2006-03-31 | 2011-07-20 | 花王株式会社 | Paper sheet manufacturing method and paper machine |
| WO2013022504A1 (en) * | 2011-05-06 | 2013-02-14 | New England Biolabs, Inc. | Ligation enhancement |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3117131A1 (en) * | 1981-04-30 | 1982-11-25 | Hoechst Ag, 6000 Frankfurt | "PLASMID PSG 2 AND METHOD FOR ITS PRODUCTION" |
| DE3128669A1 (en) * | 1981-07-20 | 1983-02-03 | Hoechst Ag, 6000 Frankfurt | "PLASMID P SVH 1 AND ITS USE" |
| US4503155A (en) * | 1982-02-01 | 1985-03-05 | Eli Lilly And Company | Multifunctional, cloning vectors for use in Streptomyces, Bacillus, and E. coli |
-
1983
- 1983-04-11 IL IL68349A patent/IL68349A0/en not_active IP Right Cessation
- 1983-04-11 ZA ZA832526A patent/ZA832526B/en unknown
- 1983-04-12 NZ NZ203864A patent/NZ203864A/en unknown
- 1983-04-12 CA CA000425651A patent/CA1207685A/en not_active Expired
- 1983-04-12 PH PH28761A patent/PH21843A/en unknown
- 1983-04-12 PT PT76536A patent/PT76536B/en unknown
- 1983-04-13 AU AU13476/83A patent/AU570631B2/en not_active Ceased
- 1983-04-13 DK DK162083A patent/DK162083A/en not_active IP Right Cessation
- 1983-04-13 FI FI831245A patent/FI831245L/en not_active Application Discontinuation
- 1983-04-14 IE IE848/83A patent/IE54818B1/en unknown
- 1983-04-14 GR GR71081A patent/GR78559B/el unknown
- 1983-04-14 DE DE8383302116T patent/DE3381699D1/en not_active Expired - Lifetime
- 1983-04-14 ES ES521468A patent/ES8500995A1/en not_active Expired
- 1983-04-14 KR KR1019830001563A patent/KR840004451A/en not_active Application Discontinuation
- 1983-04-14 GB GB08310069A patent/GB2118947B/en not_active Expired
- 1983-04-14 AT AT83302116T patent/ATE54327T1/en not_active IP Right Cessation
- 1983-04-15 JP JP58067669A patent/JPS58189198A/en active Pending
- 1983-04-15 PL PL24151183A patent/PL241511A1/en unknown
- 1983-04-15 DD DD83249906A patent/DD211360A5/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114214356A (en) * | 2021-12-28 | 2022-03-22 | 塔里木大学 | Construction method of genetic operation system of rochondrium sp TRM49605 |
Also Published As
| Publication number | Publication date |
|---|---|
| IE830848L (en) | 1983-10-16 |
| AU570631B2 (en) | 1988-03-24 |
| DK162083D0 (en) | 1983-04-13 |
| PL241511A1 (en) | 1983-12-05 |
| DK162083A (en) | 1983-10-17 |
| CA1207685A (en) | 1986-07-15 |
| IE54818B1 (en) | 1990-02-14 |
| FI831245A0 (en) | 1983-04-13 |
| PH21843A (en) | 1988-03-17 |
| GB2118947B (en) | 1985-07-03 |
| GB8310069D0 (en) | 1983-05-18 |
| ATE54327T1 (en) | 1990-07-15 |
| DD211360A5 (en) | 1984-07-11 |
| DE3381699D1 (en) | 1990-08-09 |
| PT76536B (en) | 1986-03-12 |
| KR840004451A (en) | 1984-10-15 |
| GB2118947A (en) | 1983-11-09 |
| GR78559B (en) | 1984-09-27 |
| ES521468A0 (en) | 1984-11-01 |
| ES8500995A1 (en) | 1984-11-01 |
| IL68349A0 (en) | 1983-07-31 |
| JPS58189198A (en) | 1983-11-04 |
| AU1347683A (en) | 1983-10-20 |
| FI831245L (en) | 1983-10-17 |
| NZ203864A (en) | 1986-08-08 |
| PT76536A (en) | 1983-05-01 |
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