ES8306176A1 - Procedimiento para la preparacion de un vector hibrido y la clonacion de adn-ajeno en organismos hospetantes. - Google Patents
Procedimiento para la preparacion de un vector hibrido y la clonacion de adn-ajeno en organismos hospetantes.Info
- Publication number
- ES8306176A1 ES8306176A1 ES513947A ES513947A ES8306176A1 ES 8306176 A1 ES8306176 A1 ES 8306176A1 ES 513947 A ES513947 A ES 513947A ES 513947 A ES513947 A ES 513947A ES 8306176 A1 ES8306176 A1 ES 8306176A1
- Authority
- ES
- Spain
- Prior art keywords
- svh
- plasmid
- megadaltons
- kilobases
- dsm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/886—Streptomyces
- Y10S435/906—Streptomyces venezuelae
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Peptides Or Proteins (AREA)
Abstract
PROCEDIMIENTO PARA LA OBTENCION DE VECTORES HIBRIDOS Y PARA LA CLONACION DE ADN AJENO EN ORGANISMOS HOSPEDANTES. CONSISTE EN AISLAR EL PLASMIDO P SVH 1 CON UN PESO MOLECULAR DE 8,4 MEGADALTONES, UNA LONGITUD DE CONTORNO DE 4,1 ~LM Y UN TAMAÑO MOLECULAR DE 12,6 KILOBASES, A PARTIR DE UN CULTIVO DE STREPTOMYCES VENEZUELAS DSM 40755; ABRIR EL PLASMIDO CON UNA ENZIMA DE RESTRICCION, LIGARLO CON ADN AJENO Y TRANSFORMARLO EN UN ORGANISMO HOSPEDANTE ADECUADO. TIENE APLICACIONES PARA LA OBTENCION DE ANTIBIOTICOS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19813128669 DE3128669A1 (de) | 1981-07-20 | 1981-07-20 | "plasmid p svh 1 und seine verwendung" |
Publications (2)
Publication Number | Publication Date |
---|---|
ES513947A0 ES513947A0 (es) | 1983-05-16 |
ES8306176A1 true ES8306176A1 (es) | 1983-05-16 |
Family
ID=6137332
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES513947A Expired ES8306176A1 (es) | 1981-07-20 | 1982-07-14 | Procedimiento para la preparacion de un vector hibrido y la clonacion de adn-ajeno en organismos hospetantes. |
Country Status (11)
Country | Link |
---|---|
US (1) | US4673642A (es) |
EP (1) | EP0070522B1 (es) |
JP (1) | JPS5824594A (es) |
AT (1) | ATE23190T1 (es) |
AU (1) | AU550419B2 (es) |
CA (1) | CA1187824A (es) |
DE (2) | DE3128669A1 (es) |
DK (1) | DK160999C (es) |
ES (1) | ES8306176A1 (es) |
IL (1) | IL66344A (es) |
NO (1) | NO822490L (es) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ZA832526B (en) * | 1982-04-16 | 1984-11-28 | Lilly Co Eli | Chimeric cloning vectors for use in streptomyces and e.coli |
CA1203185A (en) * | 1982-06-03 | 1986-04-15 | Thomas G. Eckhardt | Cloned streptomycete gene |
US4703009A (en) * | 1983-03-08 | 1987-10-27 | Merck & Co., Inc. | RDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes |
DE3477936D1 (en) * | 1983-03-08 | 1989-06-01 | Merck & Co Inc | Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes |
DE3412093A1 (de) * | 1984-03-31 | 1985-10-10 | Hoechst Ag, 6230 Frankfurt | Hybridplasmide mit einem streptomyceten- und einem escherichia coli-replicon |
AU594161B2 (en) * | 1986-06-24 | 1990-03-01 | Enterovax Limited | Non-antibiotic marker system |
KR960015610B1 (ko) * | 1987-06-22 | 1996-11-18 | 제네틱스 인스티튜트, 인코포레이티드 | 신규의 혈전붕괴 단백질의 제조방법 |
PT1200578E (pt) * | 1999-07-23 | 2004-08-31 | Genentech Inc | Metodo para purificacao de adn de plasmideo sem solventes organicos e sem arnase utilizando filtracao de fluxo tangencial |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0006692A1 (en) * | 1978-06-01 | 1980-01-09 | National Research Development Corporation | Streptomyces plasmids and microorganisms containing them, their use as vectors for nucleic acid and a process for the incorporation of nucleic acids into cellular systems |
US4273875A (en) * | 1979-03-05 | 1981-06-16 | The Upjohn Company | Plasmid and process of isolating same |
US4343906A (en) * | 1979-08-21 | 1982-08-10 | The Upjohn Company | Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli containing same |
US4340674A (en) * | 1980-05-05 | 1982-07-20 | The Upjohn Company | Cointegrate plasmids and their construction from plasmids of Escherichia and Streptomyces |
US4332900A (en) * | 1980-10-01 | 1982-06-01 | The Upjohn Company | Construction of co-integrate plasmids from plasmids of Streptomyces and Escherichia |
EP0058002A3 (en) * | 1981-01-26 | 1983-06-29 | The Upjohn Company | Process for stabilising potential plasmid vectors and novel plasmids |
US4401761A (en) * | 1981-01-26 | 1983-08-30 | The Upjohn Company | Process for stabilizing plasmids by deletion of DNA |
-
1981
- 1981-07-20 DE DE19813128669 patent/DE3128669A1/de not_active Withdrawn
-
1982
- 1982-07-14 ES ES513947A patent/ES8306176A1/es not_active Expired
- 1982-07-15 DE DE8282106361T patent/DE3273995D1/de not_active Expired
- 1982-07-15 AT AT82106361T patent/ATE23190T1/de active
- 1982-07-15 EP EP82106361A patent/EP0070522B1/de not_active Expired
- 1982-07-16 CA CA000407435A patent/CA1187824A/en not_active Expired
- 1982-07-19 DK DK324082A patent/DK160999C/da active
- 1982-07-19 JP JP57124553A patent/JPS5824594A/ja active Granted
- 1982-07-19 AU AU86173/82A patent/AU550419B2/en not_active Ceased
- 1982-07-19 NO NO822490A patent/NO822490L/no unknown
- 1982-07-19 IL IL66344A patent/IL66344A/xx unknown
-
1984
- 1984-08-20 US US06/642,742 patent/US4673642A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
CA1187824A (en) | 1985-05-28 |
IL66344A (en) | 1985-08-30 |
DE3128669A1 (de) | 1983-02-03 |
ATE23190T1 (de) | 1986-11-15 |
DE3273995D1 (en) | 1986-12-04 |
US4673642A (en) | 1987-06-16 |
JPH0363357B2 (es) | 1991-09-30 |
JPS5824594A (ja) | 1983-02-14 |
AU550419B2 (en) | 1986-03-20 |
EP0070522B1 (de) | 1986-10-29 |
ES513947A0 (es) | 1983-05-16 |
DK160999C (da) | 1991-11-18 |
EP0070522A3 (en) | 1983-06-22 |
IL66344A0 (en) | 1982-11-30 |
DK324082A (da) | 1983-01-21 |
NO822490L (no) | 1983-01-21 |
AU8617382A (en) | 1983-01-27 |
DK160999B (da) | 1991-05-13 |
EP0070522A2 (de) | 1983-01-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FD1A | Patent lapsed |
Effective date: 19970203 |