ES8306176A1 - Procedimiento para la preparacion de un vector hibrido y la clonacion de adn-ajeno en organismos hospetantes. - Google Patents

Procedimiento para la preparacion de un vector hibrido y la clonacion de adn-ajeno en organismos hospetantes.

Info

Publication number
ES8306176A1
ES8306176A1 ES513947A ES513947A ES8306176A1 ES 8306176 A1 ES8306176 A1 ES 8306176A1 ES 513947 A ES513947 A ES 513947A ES 513947 A ES513947 A ES 513947A ES 8306176 A1 ES8306176 A1 ES 8306176A1
Authority
ES
Spain
Prior art keywords
svh
plasmid
megadaltons
kilobases
dsm
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES513947A
Other languages
English (en)
Other versions
ES513947A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Publication of ES513947A0 publication Critical patent/ES513947A0/es
Publication of ES8306176A1 publication Critical patent/ES8306176A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/886—Streptomyces
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/886—Streptomyces
    • Y10S435/906—Streptomyces venezuelae

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE VECTORES HIBRIDOS Y PARA LA CLONACION DE ADN AJENO EN ORGANISMOS HOSPEDANTES. CONSISTE EN AISLAR EL PLASMIDO P SVH 1 CON UN PESO MOLECULAR DE 8,4 MEGADALTONES, UNA LONGITUD DE CONTORNO DE 4,1 ~LM Y UN TAMAÑO MOLECULAR DE 12,6 KILOBASES, A PARTIR DE UN CULTIVO DE STREPTOMYCES VENEZUELAS DSM 40755; ABRIR EL PLASMIDO CON UNA ENZIMA DE RESTRICCION, LIGARLO CON ADN AJENO Y TRANSFORMARLO EN UN ORGANISMO HOSPEDANTE ADECUADO. TIENE APLICACIONES PARA LA OBTENCION DE ANTIBIOTICOS.
ES513947A 1981-07-20 1982-07-14 Procedimiento para la preparacion de un vector hibrido y la clonacion de adn-ajeno en organismos hospetantes. Expired ES8306176A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19813128669 DE3128669A1 (de) 1981-07-20 1981-07-20 "plasmid p svh 1 und seine verwendung"

Publications (2)

Publication Number Publication Date
ES513947A0 ES513947A0 (es) 1983-05-16
ES8306176A1 true ES8306176A1 (es) 1983-05-16

Family

ID=6137332

Family Applications (1)

Application Number Title Priority Date Filing Date
ES513947A Expired ES8306176A1 (es) 1981-07-20 1982-07-14 Procedimiento para la preparacion de un vector hibrido y la clonacion de adn-ajeno en organismos hospetantes.

Country Status (11)

Country Link
US (1) US4673642A (es)
EP (1) EP0070522B1 (es)
JP (1) JPS5824594A (es)
AT (1) ATE23190T1 (es)
AU (1) AU550419B2 (es)
CA (1) CA1187824A (es)
DE (2) DE3128669A1 (es)
DK (1) DK160999C (es)
ES (1) ES8306176A1 (es)
IL (1) IL66344A (es)
NO (1) NO822490L (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA832526B (en) * 1982-04-16 1984-11-28 Lilly Co Eli Chimeric cloning vectors for use in streptomyces and e.coli
CA1203185A (en) * 1982-06-03 1986-04-15 Thomas G. Eckhardt Cloned streptomycete gene
US4703009A (en) * 1983-03-08 1987-10-27 Merck & Co., Inc. RDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes
DE3477936D1 (en) * 1983-03-08 1989-06-01 Merck & Co Inc Recombinant dna cloning vector pve1, deletion and hybrid mutants, and recombinant derivatives thereof, products and processes
DE3412093A1 (de) * 1984-03-31 1985-10-10 Hoechst Ag, 6230 Frankfurt Hybridplasmide mit einem streptomyceten- und einem escherichia coli-replicon
AU594161B2 (en) * 1986-06-24 1990-03-01 Enterovax Limited Non-antibiotic marker system
KR960015610B1 (ko) * 1987-06-22 1996-11-18 제네틱스 인스티튜트, 인코포레이티드 신규의 혈전붕괴 단백질의 제조방법
PT1200578E (pt) * 1999-07-23 2004-08-31 Genentech Inc Metodo para purificacao de adn de plasmideo sem solventes organicos e sem arnase utilizando filtracao de fluxo tangencial

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0006692A1 (en) * 1978-06-01 1980-01-09 National Research Development Corporation Streptomyces plasmids and microorganisms containing them, their use as vectors for nucleic acid and a process for the incorporation of nucleic acids into cellular systems
US4273875A (en) * 1979-03-05 1981-06-16 The Upjohn Company Plasmid and process of isolating same
US4343906A (en) * 1979-08-21 1982-08-10 The Upjohn Company Hybrid plasmid of pBR322 and Streptomyces plasmid and E. coli containing same
US4340674A (en) * 1980-05-05 1982-07-20 The Upjohn Company Cointegrate plasmids and their construction from plasmids of Escherichia and Streptomyces
US4332900A (en) * 1980-10-01 1982-06-01 The Upjohn Company Construction of co-integrate plasmids from plasmids of Streptomyces and Escherichia
EP0058002A3 (en) * 1981-01-26 1983-06-29 The Upjohn Company Process for stabilising potential plasmid vectors and novel plasmids
US4401761A (en) * 1981-01-26 1983-08-30 The Upjohn Company Process for stabilizing plasmids by deletion of DNA

Also Published As

Publication number Publication date
CA1187824A (en) 1985-05-28
IL66344A (en) 1985-08-30
DE3128669A1 (de) 1983-02-03
ATE23190T1 (de) 1986-11-15
DE3273995D1 (en) 1986-12-04
US4673642A (en) 1987-06-16
JPH0363357B2 (es) 1991-09-30
JPS5824594A (ja) 1983-02-14
AU550419B2 (en) 1986-03-20
EP0070522B1 (de) 1986-10-29
ES513947A0 (es) 1983-05-16
DK160999C (da) 1991-11-18
EP0070522A3 (en) 1983-06-22
IL66344A0 (en) 1982-11-30
DK324082A (da) 1983-01-21
NO822490L (no) 1983-01-21
AU8617382A (en) 1983-01-27
DK160999B (da) 1991-05-13
EP0070522A2 (de) 1983-01-26

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970203