JPS58210025A - 抗トレハラ−ゼ抗体 - Google Patents
抗トレハラ−ゼ抗体Info
- Publication number
- JPS58210025A JPS58210025A JP9221782A JP9221782A JPS58210025A JP S58210025 A JPS58210025 A JP S58210025A JP 9221782 A JP9221782 A JP 9221782A JP 9221782 A JP9221782 A JP 9221782A JP S58210025 A JPS58210025 A JP S58210025A
- Authority
- JP
- Japan
- Prior art keywords
- trehalase
- antibody
- antigen
- animal
- purified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102100029677 Trehalase Human genes 0.000 claims abstract description 50
- 108010087472 Trehalase Proteins 0.000 claims abstract description 50
- 241001465754 Metazoa Species 0.000 claims abstract description 14
- 239000000427 antigen Substances 0.000 claims abstract description 14
- 102000036639 antigens Human genes 0.000 claims abstract description 14
- 108091007433 antigens Proteins 0.000 claims abstract description 14
- 241000283973 Oryctolagus cuniculus Species 0.000 abstract description 13
- 210000002966 serum Anatomy 0.000 abstract description 8
- 210000002700 urine Anatomy 0.000 abstract description 8
- 238000002347 injection Methods 0.000 abstract description 7
- 239000007924 injection Substances 0.000 abstract description 7
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract description 6
- 235000011130 ammonium sulphate Nutrition 0.000 abstract description 6
- 239000000839 emulsion Substances 0.000 abstract description 6
- 238000004255 ion exchange chromatography Methods 0.000 abstract description 5
- 239000002671 adjuvant Substances 0.000 abstract description 4
- 210000004369 blood Anatomy 0.000 abstract description 4
- 239000008280 blood Substances 0.000 abstract description 4
- 238000005185 salting out Methods 0.000 abstract description 4
- 210000001124 body fluid Anatomy 0.000 abstract description 3
- 239000010839 body fluid Substances 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 3
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 2
- 230000001804 emulsifying effect Effects 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 241000894007 species Species 0.000 abstract description 2
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 206010033675 panniculitis Diseases 0.000 abstract 1
- 239000011369 resultant mixture Substances 0.000 abstract 1
- 210000004304 subcutaneous tissue Anatomy 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 abstract 1
- 210000003734 kidney Anatomy 0.000 description 14
- 238000000034 method Methods 0.000 description 12
- 230000002485 urinary effect Effects 0.000 description 12
- 108090000790 Enzymes Proteins 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 8
- 239000000243 solution Substances 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 238000000502 dialysis Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000002523 gelfiltration Methods 0.000 description 3
- 238000004062 sedimentation Methods 0.000 description 3
- 210000000813 small intestine Anatomy 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000000951 immunodiffusion Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
- 206010011878 Deafness Diseases 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 238000011387 Li's method Methods 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 241001474791 Proboscis Species 0.000 description 1
- 239000012506 Sephacryl® Substances 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 206010044565 Tremor Diseases 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 238000005377 adsorption chromatography Methods 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 210000000702 aorta abdominal Anatomy 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000000760 immunoelectrophoresis Methods 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000009169 immunotherapy Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9221782A JPS58210025A (ja) | 1982-06-01 | 1982-06-01 | 抗トレハラ−ゼ抗体 |
| FR8308461A FR2527223B1 (fr) | 1982-05-24 | 1983-05-20 | Anticorps anti-trehalase, reactif et procede de determination immuno-enzymatique de la trehalase |
| DE19833318761 DE3318761A1 (de) | 1982-05-24 | 1983-05-24 | Anti-trehalase antikoerper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9221782A JPS58210025A (ja) | 1982-06-01 | 1982-06-01 | 抗トレハラ−ゼ抗体 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58210025A true JPS58210025A (ja) | 1983-12-07 |
| JPH0370786B2 JPH0370786B2 (enrdf_load_stackoverflow) | 1991-11-08 |
Family
ID=14048272
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9221782A Granted JPS58210025A (ja) | 1982-05-24 | 1982-06-01 | 抗トレハラ−ゼ抗体 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS58210025A (enrdf_load_stackoverflow) |
-
1982
- 1982-06-01 JP JP9221782A patent/JPS58210025A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0370786B2 (enrdf_load_stackoverflow) | 1991-11-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPS60185800A (ja) | モノクロナ−ル抗体 | |
| WO1983004312A1 (en) | Monoclonal antibody mixtures and use thereof for enhanced sensitivity immunoassays | |
| EP0067642A1 (en) | Detection of malignant tumor cells | |
| Veltri et al. | Isolation and identification of human lung tumor-associated antigens | |
| NO159619B (no) | Immunokjemisk enzymbestemmelse. | |
| EP0020090A1 (en) | Radioimmunoassay of migration inhibitory factor | |
| Klein et al. | IgM–IgG cryoglobulinaemia with IgM paraprotein component | |
| KR910008637B1 (ko) | 심근 미오신 중사슬에 대한 단일클론항체 | |
| JPS58210025A (ja) | 抗トレハラ−ゼ抗体 | |
| JPS62108157A (ja) | 抗体 | |
| JPS63243098A (ja) | ヒトおよび動物組織からの基底膜タンパクの単離方法 | |
| JP4178632B2 (ja) | 干渉作用を回避する方法及び試薬 | |
| JPS60190722A (ja) | 抗ヒト肺癌単クロ−ン性抗体 | |
| JPH02221300A (ja) | イムノアフィニティーマトリックス | |
| JP4215462B2 (ja) | 非グリコシル化ヘモグロビン、グリコシル化ヘモグロビンに特異的に結合するモノクローナル抗体の製造方法 | |
| JPS585661A (ja) | 前立腺酸ホスフアタ−ゼアイソザイムの定量方法 | |
| CN112094816B (zh) | 分泌2-巯基苯并咪唑单克隆抗体的杂交瘤细胞株、单克隆抗体及应用 | |
| EP0178779B1 (en) | Reagents for measurement of immune complexes and method for measurement of immune complexes by use thereof | |
| JPH09274038A (ja) | 糖化ヘモグロビンの測定方法 | |
| JPS62261961A (ja) | 抗イデイオタイプ抗体を用いた抗ヒト肝特異抗原抗体量の測定方法および肝疾患診断への応用 | |
| SU1158029A3 (ru) | Способ количественного определени трийодтиронина | |
| Das et al. | A method for production of antibody to hepatitis‐associated antigen in rabbits | |
| JP2553343B2 (ja) | 癌抗体の測定法 | |
| Ganrot | How apparently identical antisera can give apparently different results | |
| Singh | Development of an Enzyme Linked Immunosorbent Assay Using Monoclonal Antibodies for Detecting Antibodies to Pasteurella Multocida |