JPS57198089A - Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same - Google Patents

Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same

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Publication number
JPS57198089A
JPS57198089A JP8098781A JP8098781A JPS57198089A JP S57198089 A JPS57198089 A JP S57198089A JP 8098781 A JP8098781 A JP 8098781A JP 8098781 A JP8098781 A JP 8098781A JP S57198089 A JPS57198089 A JP S57198089A
Authority
JP
Japan
Prior art keywords
hemagglutinin
strain
agglutininless
erythrocyte
fresh
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP8098781A
Other languages
Japanese (ja)
Other versions
JPH0221800B2 (en
Inventor
Katsumi Kume
Akira Sawada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KITAZATO KENKYUSHO
Kitasato Institute
Original Assignee
KITAZATO KENKYUSHO
Kitasato Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KITAZATO KENKYUSHO, Kitasato Institute filed Critical KITAZATO KENKYUSHO
Priority to JP8098781A priority Critical patent/JPS57198089A/en
Publication of JPS57198089A publication Critical patent/JPS57198089A/en
Publication of JPH0221800B2 publication Critical patent/JPH0221800B2/ja
Granted legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)

Abstract

PURPOSE: To prepare a hemagglutinin which can be used specifically for the determination of the hemagglutinin-suppressive antibody to the fresh erythrocyte- agglutininless strain of Haemophilus paragallinarum.
CONSTITUTION: Fresh erythrocyte-agglutininless strain of Haemophilus paragallinarum, especially a serotype-2 strain, such as Haemophilus paragallinarum H- 18, is cultured in a proper liquid medium, and the resultant microbial dispersion is treated with an aqueous solution of potassium rhodanide containing sodium chloride and having a sodium chloride concentration of 0.1W0.6M, a potassium rhodanide concentration of 0.25W1.5M and a pH of 5.0W7.0. The treated dispersion is further treated with sonic wave of 10KC at 2W5°C for 1W15min to obtain a hemagglutinin which has low agglutinating property to fresh erythrocytes and high agglutinating property to erythrocytes fixed with glutaraldehyde. The hemagglutinin antibody can be determined by using said hemagglutinin as the antigen, and a dispersion of erythrocytes fixed with glutaraldehyde as the erythrocyte dispersion.
COPYRIGHT: (C)1982,JPO&Japio
JP8098781A 1981-05-29 1981-05-29 Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same Granted JPS57198089A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP8098781A JPS57198089A (en) 1981-05-29 1981-05-29 Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP8098781A JPS57198089A (en) 1981-05-29 1981-05-29 Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP29327487A Division JPS64467A (en) 1987-11-20 1987-11-20 Measuring system using hemagglutinin of agglutinin deficient strain to fresh red blood cells of haemophilus paragallinarum

Publications (2)

Publication Number Publication Date
JPS57198089A true JPS57198089A (en) 1982-12-04
JPH0221800B2 JPH0221800B2 (en) 1990-05-16

Family

ID=13733844

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8098781A Granted JPS57198089A (en) 1981-05-29 1981-05-29 Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same

Country Status (1)

Country Link
JP (1) JPS57198089A (en)

Also Published As

Publication number Publication date
JPH0221800B2 (en) 1990-05-16

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