JPS57198089A - Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same - Google Patents
Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the sameInfo
- Publication number
- JPS57198089A JPS57198089A JP8098781A JP8098781A JPS57198089A JP S57198089 A JPS57198089 A JP S57198089A JP 8098781 A JP8098781 A JP 8098781A JP 8098781 A JP8098781 A JP 8098781A JP S57198089 A JPS57198089 A JP S57198089A
- Authority
- JP
- Japan
- Prior art keywords
- hemagglutinin
- strain
- agglutininless
- erythrocyte
- fresh
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
PURPOSE: To prepare a hemagglutinin which can be used specifically for the determination of the hemagglutinin-suppressive antibody to the fresh erythrocyte- agglutininless strain of Haemophilus paragallinarum.
CONSTITUTION: Fresh erythrocyte-agglutininless strain of Haemophilus paragallinarum, especially a serotype-2 strain, such as Haemophilus paragallinarum H- 18, is cultured in a proper liquid medium, and the resultant microbial dispersion is treated with an aqueous solution of potassium rhodanide containing sodium chloride and having a sodium chloride concentration of 0.1W0.6M, a potassium rhodanide concentration of 0.25W1.5M and a pH of 5.0W7.0. The treated dispersion is further treated with sonic wave of 10KC at 2W5°C for 1W15min to obtain a hemagglutinin which has low agglutinating property to fresh erythrocytes and high agglutinating property to erythrocytes fixed with glutaraldehyde. The hemagglutinin antibody can be determined by using said hemagglutinin as the antigen, and a dispersion of erythrocytes fixed with glutaraldehyde as the erythrocyte dispersion.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8098781A JPS57198089A (en) | 1981-05-29 | 1981-05-29 | Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP8098781A JPS57198089A (en) | 1981-05-29 | 1981-05-29 | Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP29327487A Division JPS64467A (en) | 1987-11-20 | 1987-11-20 | Measuring system using hemagglutinin of agglutinin deficient strain to fresh red blood cells of haemophilus paragallinarum |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS57198089A true JPS57198089A (en) | 1982-12-04 |
JPH0221800B2 JPH0221800B2 (en) | 1990-05-16 |
Family
ID=13733844
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP8098781A Granted JPS57198089A (en) | 1981-05-29 | 1981-05-29 | Preparation of hemagglutinin of fresh erythrocyte- agglutininless strain of haemophilus paragallinarum, and determination system using the same |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS57198089A (en) |
-
1981
- 1981-05-29 JP JP8098781A patent/JPS57198089A/en active Granted
Also Published As
Publication number | Publication date |
---|---|
JPH0221800B2 (en) | 1990-05-16 |
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