JPS56120951A - Measuring method for blood hemoglobin - Google Patents

Measuring method for blood hemoglobin

Info

Publication number
JPS56120951A
JPS56120951A JP2451480A JP2451480A JPS56120951A JP S56120951 A JPS56120951 A JP S56120951A JP 2451480 A JP2451480 A JP 2451480A JP 2451480 A JP2451480 A JP 2451480A JP S56120951 A JPS56120951 A JP S56120951A
Authority
JP
Japan
Prior art keywords
blood
aliphatic high
methemoglobin
approx
reagent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2451480A
Other languages
Japanese (ja)
Inventor
Yoshinobu Miyashita
Tadashi Hamanaka
Shinji Satomura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Wako Pure Chemical Corp
Original Assignee
Wako Pure Chemical Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wako Pure Chemical Industries Ltd filed Critical Wako Pure Chemical Industries Ltd
Priority to JP2451480A priority Critical patent/JPS56120951A/en
Publication of JPS56120951A publication Critical patent/JPS56120951A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin

Abstract

PURPOSE:To quantify blood hemoglobin precisely and easily without using an oxided substance such as potassium ferricyanide by optically measuring bound material of methemoglobin and aliphatic high sulfonate. CONSTITUTION:The aliphatic high sulfonate (ex. dodecane sulfonic acid sodium) shown by the expression (R is alkyl of C11-16; M is a positive ion of m value) is solved in water or buffer liquid with pH 5-10 approx., or shortly, nonionic surfactant being added, and thereby a reagent is formed. Next, the sample blood (or diluted liquid with physiological salt solution) and the aforesaid reagent are mixed at the normal temperature and the bound material of methemoglobin and aliphatic high sulfonate thus produced is quantified with comparison of color in the vicinity of wavelength 534nm. In addition, the aforesaid method unnecessitates the combined use of a hemolytic agent and is free from variation of an absorption curve within the range of pH 5-10 approx. and from the influence of albumen, bilirubin, hyperlipemia blood, white corpuscle and the like.
JP2451480A 1980-02-28 1980-02-28 Measuring method for blood hemoglobin Pending JPS56120951A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2451480A JPS56120951A (en) 1980-02-28 1980-02-28 Measuring method for blood hemoglobin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2451480A JPS56120951A (en) 1980-02-28 1980-02-28 Measuring method for blood hemoglobin

Publications (1)

Publication Number Publication Date
JPS56120951A true JPS56120951A (en) 1981-09-22

Family

ID=12140272

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2451480A Pending JPS56120951A (en) 1980-02-28 1980-02-28 Measuring method for blood hemoglobin

Country Status (1)

Country Link
JP (1) JPS56120951A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60168050A (en) * 1984-02-10 1985-08-31 Wako Pure Chem Ind Ltd Method for averting influence of hemoglobin
JPS60227171A (en) * 1984-04-25 1985-11-12 Sogo Seibutsu Igaku Kenkyusho:Kk Measuring method of glucose conjugated to hemoglobin
EP0184787A2 (en) * 1984-12-06 1986-06-18 TECHNICON INSTRUMENTS CORPORATION(a Delaware corporation) Cyanide-free hemoglobin reagent

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60168050A (en) * 1984-02-10 1985-08-31 Wako Pure Chem Ind Ltd Method for averting influence of hemoglobin
JPH0358467B2 (en) * 1984-02-10 1991-09-05 Wako Pure Chem Ind Ltd
JPS60227171A (en) * 1984-04-25 1985-11-12 Sogo Seibutsu Igaku Kenkyusho:Kk Measuring method of glucose conjugated to hemoglobin
JPH0414311B2 (en) * 1984-04-25 1992-03-12 Bii Emu Eru Kk
EP0184787A2 (en) * 1984-12-06 1986-06-18 TECHNICON INSTRUMENTS CORPORATION(a Delaware corporation) Cyanide-free hemoglobin reagent

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