GB1183499A - Biological Stains - Google Patents
Biological StainsInfo
- Publication number
- GB1183499A GB1183499A GB4390967A GB4390967A GB1183499A GB 1183499 A GB1183499 A GB 1183499A GB 4390967 A GB4390967 A GB 4390967A GB 4390967 A GB4390967 A GB 4390967A GB 1183499 A GB1183499 A GB 1183499A
- Authority
- GB
- United Kingdom
- Prior art keywords
- solution
- eosin
- methylene blue
- polychrome
- wavelength
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 abstract 6
- 229960000907 methylthioninium chloride Drugs 0.000 abstract 6
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 abstract 5
- PLXMOAALOJOTIY-FPTXNFDTSA-N Aesculin Natural products OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)[C@H]1Oc2cc3C=CC(=O)Oc3cc2O PLXMOAALOJOTIY-FPTXNFDTSA-N 0.000 abstract 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 abstract 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract 2
- 238000001914 filtration Methods 0.000 abstract 2
- 230000003287 optical effect Effects 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 abstract 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 abstract 1
- 230000032683 aging Effects 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 238000007865 diluting Methods 0.000 abstract 1
- ZBQZBWKNGDEDOA-UHFFFAOYSA-N eosin B Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC([N+]([O-])=O)=C(O)C(Br)=C1OC1=C2C=C([N+]([O-])=O)C(O)=C1Br ZBQZBWKNGDEDOA-UHFFFAOYSA-N 0.000 abstract 1
- SEACYXSIPDVVMV-UHFFFAOYSA-L eosin Y Chemical compound [Na+].[Na+].[O-]C(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C([O-])=C(Br)C=C21 SEACYXSIPDVVMV-UHFFFAOYSA-L 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 239000008057 potassium phosphate buffer Substances 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- 229910000029 sodium carbonate Inorganic materials 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
- 239000001117 sulphuric acid Substances 0.000 abstract 1
- 235000011149 sulphuric acid Nutrition 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
1,183,499. Preparing microscope specimens. BAXTER LABORATORIES Inc. Oct. 31, 1967 [Dec. 5, 1966], No. 49309/67. Heading G2J. A reagent for staining biological specimens, such as blood, for microscopic examination, comprises:- (a) a polychrome methylene blue solution such that when diluted with water to a concentration of one part of polychrome methylene blue solution in 2, 000 parts of total solution the ratio of the spectrophotometrically determined optical density in a 1 centimetre cell at a wavelength of 660 mÁto that at a wavelength of 620 mÁ is between 0À8 and 1À5, and (b) an eosin solution such that when diluted in water to a concentration of one part of eosin solution in 100 parts of total solution the spectrophotometrically determined optical density in a 1 centimetre cell at a wavelength of 520mÁ is between 0À3 and 0À7. In an example, the polychrome methylene blue solution is prepared by heating a solution of 1% methylene blue and 0À25% sodium carbonate to 95‹C. for four hours, filtering, ageing and adjusting the pH to 6À85 using sulphuric acid, and diluting the 1% methylene blue to 0À8% with potassium phosphate buffer having a pH of 6À9. The eosin solution is prepared by adding equal amounts of eosin Y and eosin B to a solution of Na 2 HPO 4 and Na H 2 PO 4 to provide a 0À05% solution of eosin, storing, adding 0À1% sodium azide to prevent bacterial growth and filtering. To stain a biological specimen it is immersed on a microscope slide in the polychrome methylene blue solution for 10-20 seconds, thoroughly rinsed, immersed for 10-20 seconds in the eosin solution, rinsed again and then dried in air.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US59889966A | 1966-12-05 | 1966-12-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1183499A true GB1183499A (en) | 1970-03-04 |
Family
ID=24397388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4390967A Expired GB1183499A (en) | 1966-12-05 | 1967-10-31 | Biological Stains |
Country Status (3)
| Country | Link |
|---|---|
| BE (1) | BE707093A (en) |
| DE (1) | DE1648852B2 (en) |
| GB (1) | GB1183499A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0086951A1 (en) * | 1982-02-01 | 1983-08-31 | Miles Laboratories, Inc. | Stabilized Romanowsky stain solution and method of preparation thereof |
| WO2014118041A1 (en) * | 2013-02-04 | 2014-08-07 | Siemens Aktiengesellschaft | Stabilization of aqueous xanthene dye solutions by means of antichaotropic compounds |
| CN107664595A (en) * | 2016-07-27 | 2018-02-06 | 石家庄晟科生物科技有限公司 | Efficient chromosomal G-banding staining kit |
-
1967
- 1967-10-31 GB GB4390967A patent/GB1183499A/en not_active Expired
- 1967-11-27 BE BE707093D patent/BE707093A/xx unknown
- 1967-12-01 DE DE1967B0095666 patent/DE1648852B2/en active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0086951A1 (en) * | 1982-02-01 | 1983-08-31 | Miles Laboratories, Inc. | Stabilized Romanowsky stain solution and method of preparation thereof |
| WO2014118041A1 (en) * | 2013-02-04 | 2014-08-07 | Siemens Aktiengesellschaft | Stabilization of aqueous xanthene dye solutions by means of antichaotropic compounds |
| CN107664595A (en) * | 2016-07-27 | 2018-02-06 | 石家庄晟科生物科技有限公司 | Efficient chromosomal G-banding staining kit |
Also Published As
| Publication number | Publication date |
|---|---|
| DE1648852A1 (en) | 1971-05-13 |
| DE1648852B2 (en) | 1976-06-24 |
| BE707093A (en) | 1968-04-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |