CN107664595A - Efficient chromosomal G-banding staining kit - Google Patents
Efficient chromosomal G-banding staining kit Download PDFInfo
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- CN107664595A CN107664595A CN201610597175.4A CN201610597175A CN107664595A CN 107664595 A CN107664595 A CN 107664595A CN 201610597175 A CN201610597175 A CN 201610597175A CN 107664595 A CN107664595 A CN 107664595A
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- liquid
- banding
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- dyeing liquor
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- 230000002759 chromosomal effect Effects 0.000 title claims abstract description 50
- 238000010186 staining Methods 0.000 title claims abstract description 9
- 238000004043 dyeing Methods 0.000 claims abstract description 72
- 239000007788 liquid Substances 0.000 claims abstract description 50
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000000975 dye Substances 0.000 claims abstract description 36
- 210000000349 chromosome Anatomy 0.000 claims abstract description 33
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 24
- 235000011187 glycerol Nutrition 0.000 claims abstract description 11
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 10
- 239000000203 mixture Substances 0.000 claims abstract description 7
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims abstract description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000000049 pigment Substances 0.000 claims abstract 2
- 239000002994 raw material Substances 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- 239000012224 working solution Substances 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 3
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 abstract 1
- 229960000907 methylthioninium chloride Drugs 0.000 abstract 1
- 238000007789 sealing Methods 0.000 description 8
- 238000002738 Giemsa staining Methods 0.000 description 7
- 230000000007 visual effect Effects 0.000 description 7
- 230000002093 peripheral effect Effects 0.000 description 6
- 238000001914 filtration Methods 0.000 description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 238000004040 coloring Methods 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 208000011359 Chromosome disease Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 208000024971 chromosomal disease Diseases 0.000 description 2
- 238000003759 clinical diagnosis Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- NALREUIWICQLPS-UHFFFAOYSA-N 7-imino-n,n-dimethylphenothiazin-3-amine;hydrochloride Chemical compound [Cl-].C1=C(N)C=C2SC3=CC(=[N+](C)C)C=CC3=NC2=C1 NALREUIWICQLPS-UHFFFAOYSA-N 0.000 description 1
- 108010019160 Pancreatin Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 241000224016 Plasmodium Species 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 230000031016 anaphase Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 210000003917 human chromosome Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 229940055695 pancreatin Drugs 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides a kind of chromosomal G-banding staining kit.Dyeing liquor A liquid is mainly the mixtures such as reddish black pigment, Yihong, methylene blue, methanol, glycerine.Dyeing liquor B liquid is mainly potassium dihydrogen phosphate, disodium hydrogen phosphate.Dyeing liquor of the present invention is specifically applied to chromosomal G-banding, and cost is low, and dyeing is fast and the chromosomal G-banding band that dyes is clear, easily identification, is adapted to the hospital for carrying out chromosome examination project to use.
Description
Technical field
The invention belongs to a kind of biological reagent in genetic diagnosis field, more particularly, to the special Jim Sa of chromosomal G-banding
Dye each group distribution ratio of dyeing liquor A liquid and dyeing liquor B liquid in liquid kit.
Background technology
Human chromosomal is in homogeneous shape with Jim Sa dyeing, but if chromosome is by denaturation and (or) enzymic digestion
After different disposal, then dye can present a series of depths alternately band line, these band line figures be referred to as chromosome banding pattern.Aobvious band
Technology is exactly to colour the different zones of chromosome by special colouring method, chromosome is showed light and shade phase under light microscopic
Between band line.Each chromosome has specific band line, or even the long-armed and galianconism of each chromosome has specificity.According to dye
The different banding patterns of colour solid, can individual character that is more careful and reliably identifying chromosome.The specific banding pattern of chromosome changes, then
Represent that the structure of the chromosome is changed, be usually used in clinical diagnosis.Ordinary stain body banding technique has the aobvious bands of G (most normal
With), nuclei dyeing can be dyed pink by Giemsa stain into aubergine or bluish violet, endochylema, and it is clear to be showed under light microscopic
Cell.Processing chromosome is carried out with protease etc. before dye, is then dyed with Ji's nurse Sa dye liquor, on chromosome, can gone out again
Existing different deep or light band sample colorings, show chromosome image under light microscopic.G Banded methods are simple, and band line is clear, chromosome
Sample can preserve for a long time, therefore be widely used in the diagnosis and research of chromosomal disorder.
Chromosomal G-banding detection in, at present still dedicated for chromosomal G-banding Giemsa staining liquid to dye
Body sample is dyed, and is simply dyed with traditional Giemsa staining liquid, secondly the unstable effect of reagent of each producer
Otherness is big, and also dyeing time is grown, and Color is bad, and above mentioned problem is to clinician and research worker in post analysis
Difficulty is brought when as a result.The means that chromosomal G-banding karyotyping has diagnosed as the chromosomal disorder of routine clinical application.It is badly in need of
Want that a kind of cost is cheap, Color is good, the convenient chromosomal G-banding dyeing liquor of application method, this has weight to clinical diagnosis
The practical significance wanted.
The content of the invention
In order to solve above-mentioned existing issue, present invention aims at the Jim Sa dye provided dedicated for chromosomal G-banding
Color liquid.
The technical solution adopted in the present invention is as follows：
Efficient chromosomal G-banding staining kit, dye liquor used are as follows：
The each component of dyeing liquor A liquid is as follows：
| Component | Parts by weight | Parts by volume |
| Jim Sa pulvis | 2 | ----- |
| Glycerine | ----- | 80 |
| Methanol | ----- | 120 |
Wherein described parts by weight are corresponding with g, and parts by volume is corresponding with ml.
Wherein described methanol is pure to analyze.
Wherein, it is prepared by the raw material of following proportionings：
1）Weigh the raw material of each proportioning；
2）Jim Sa pulvis is mixed with glycerine, grinds 3 hours, then 65 DEG C of water 4 hours；
3）After being cooled to room temperature, add the methanol of 120 parts by volume.Shaking, make it well mixed；
4）Sealing is kept in dark place, sooner or later each daily to shake 5min.First quarter moon is kept in dark place in sealing, after filtering.
The each component of dyeing liquor B liquid is as follows：
| Component | Parts by weight | Parts by volume |
| Potassium dihydrogen phosphate | 1-2 | ---- |
| Disodium hydrogen phosphate | 9-10 | ---- |
| Pure water | ---- | 1000 |
Wherein, it is prepared by the raw material of following proportionings：
1）Weigh the raw material of each proportioning：Potassium dihydrogen phosphate 1.6g potassium dihydrogen phosphates and 9.07g disodium hydrogen phosphates；
2）By above dissolution of raw material into 800ml pure water, fully dissolving, then be settled to 1000ml；
3）Room temperature preservation.
Efficient chromosomal G-banding dyeing liquid kit, it is characterized in that：It includes two kinds of mix reagents, described mixing examination
Agent includes：Dyeing liquor A liquid, dyeing liquor B liquid.
Described dyeing liquor A liquid, dyeing liquor B liquid are the component proportion described in claim 1.
Chromosomal G-banding dyeing liquor application method, it is characterized in that, concretely comprise the following steps：
1）Chromosome specimen is handled with trypsase before dyeing；
2）Chromosomal G-banding dyeing working solution is prepared：The dyeing liquor A liquid 1ml in the kit described in claim 1 are taken, then are taken
Dyeing liquor B liquid 5ml in kit described in claim 1, both the above solution is added in staining jar and is thoroughly mixed, i.e.,
Working solution is dyed for efficient chromosomal G-banding.By step 1）Handled chromosome specimen immerses chromosomal G-banding dyeing work
Liquid；
3）Chromosomal G-banding dyeing condition is as follows：
Room temperature（28-30℃）, dye 72s-4min.
The chromosomal G-banding staining kit of the present invention detects to chromosomal G-banding and karyotyping, have high efficiency and
Clearly, good resolution.Make reviewer simple to operate, make doctor to patient during diagnosing, accurate analysis, and solve
Chromosome dyeing liquid unstability, to clinician bring it is inconvenient for use the problem of.
Dye liquor of the present invention is splendid to chromosome banding Color.Inventor has found that market is existing in research process
Giemsa staining liquid is mainly used in cell and plasmodium dyeing, although to the effective dyeing of cell, chromosome banding is coloured tired
Hardly possible, Color are poor.
Dye liquor section of the present invention is effectively applied to clinical chromosome examination project, meets laboratory physician in diagnosis to dye
Existing doubt when colour solid dyeing is unintelligible.
Brief description of the drawings
Accompanying drawing 1 is present invention detection sample chromosomal G-banding photo（1000 times of visuals field）.
Accompanying drawing 2 is that traditional dye liquor detects sample chromosomal G-banding photo（1000 times of visuals field）.
Accompanying drawing 3 detects human peripheral sample chromosomal G-banding photo for control dye liquor（1000 times of visuals field）.
Accompanying drawing 4 is that dye liquor of the present invention detects human peripheral sample chromosomal G-banding photo（1000 times of visuals field）.
Accompanying drawing 5 is that dye liquor of the present invention detects human peripheral sample chromosomal G-banding photo（1000 times of visuals field）.
Embodiment
Patent application is described further in conjunction with the embodiments.
Efficient chromosomal G-banding staining kit, dye liquor used are as follows：
Jim Sa pulvis：RS-Sigma；
Methanol（AR levels）, glycerine：Tianjin great Mao；
Microscope：Olympus microscope（BX51）；
The each component of Giemsa staining liquid A liquid is as follows：
| Component | Parts by weight (g) | Parts by volume |
| Jim Sa pulvis | 1-2 | ---- |
| Glycerine | ---- | 80 |
| Methanol | ---- | 120 |
Wherein described parts by weight are corresponding with g, and parts by volume is corresponding with ml.
Wherein described methanol is pure to analyze.
Wherein, it is prepared by the raw material of following proportionings：
1）Weigh the raw material of each proportioning；
2）Jim Sa pulvis is mixed with glycerine, grinds 3 hours, then 65 DEG C of water 4 hours；
3）After being cooled to room temperature, add the methanol of 120 parts by volume.Shaking, make it well mixed；
4）Sealing is kept in dark place, sooner or later each daily to shake 5min.First quarter moon is kept in dark place in sealing, after filtering.
The each component of dyeing liquor B liquid is as follows：
| Component | Parts by weight | Parts by volume |
| Potassium dihydrogen phosphate | 1-2 | ---- |
| Disodium hydrogen phosphate | 9-10 | ---- |
| Pure water | ---- | 1000 |
Wherein, it is prepared by the raw material of following proportionings：
1）Weigh the raw material of each proportioning：Potassium dihydrogen phosphate 1.6g potassium dihydrogen phosphates and 9.07g disodium hydrogen phosphates；
2）By above dissolution of raw material into 800ml pure water, fully dissolving, then be settled to 1000ml；
3）Room temperature preservation.
Chromosomal G-banding staining kit, it is characterized in that：It includes two kinds of mix reagents, and described mix reagent includes：
Dyeing liquor A liquid, dyeing liquor B liquid.
Embodiment
Embodiment 1
1）Formula for dye liquor Jim Sa pulvis 2g, glycerine 80ml, methanol 120ml；
2）Preparation method：
Chromosomal G-banding dyeing liquor A liquid：Jim Sa pulvis 2g is taken, Jim Sa pulvis is mixed with 80ml glycerine, is ground 3 hours,
Then 65 DEG C of water 4 hours.After being cooled to room temperature, 120ml methanol is added.Shaking, make it well mixed.Sealing is kept in dark place,
Sooner or later it is each daily to shake 5min.First quarter moon is kept in dark place in sealing, after filtering.
Chromosomal G-banding dyeing liquor B liquid：
1）Weigh the raw material of each proportioning；
2）1.6g potassium dihydrogen phosphates and 9.07g disodium hydrogen phosphates are dissolved in 1000ml pure water, are completely dissolved it；
3）Room-temperature seal is kept in dark place.
Embodiment 2
1）Formula for dye liquor Jim Sa pulvis 1.5g, glycerine 80ml, methanol 120ml；
2）Preparation method：
Chromosomal G-banding dyeing liquor A liquid：Jim Sa pulvis 2g is taken, Jim Sa pulvis is mixed with 80ml glycerine, is ground 3 hours,
Then 65 DEG C of water 4 hours.After being cooled to room temperature, 120ml methanol is added.Shaking, make it well mixed.Sealing is kept in dark place,
Sooner or later it is each daily to shake 5min.First quarter moon is kept in dark place in sealing, after filtering.
Chromosomal G-banding dyeing liquor B liquid：
1）Weigh the raw material of each proportioning；
2）1.6g potassium dihydrogen phosphates and 9.00g disodium hydrogen phosphates are dissolved in 1000ml pure water, are completely dissolved it；
3）Room-temperature seal is kept in dark place.
Illustrate beneficial effects of the present invention with the mode of test example below：
Dyeing of 1 dye liquor of the present invention of test example to human peripheral chromosome：
First, test material
Dye liquor of the present invention：The chromosomal G-banding dyeing liquor prepared according to the method for embodiment 1：
Compare dye liquor：Commercially available traditional Giemsa staining liquid（Producer：Zhuhai shellfish rope lot number：414091 terms of validity：（2016-
09-08）；
Human peripheral chromosome drips piece.
2nd, colouring method
1st, by dyeing liquor A liquid（Dye liquor of the present invention or control dye liquor）Mixed with dyeing liquor B liquid：Add 5ml dyeing liquor A liquid and 25ml
Dyeing liquor B liquid, with ear washing bulb blow mix, as chromosomal G-banding dyeing working solution
2nd, dyeing liquor working solution is added：Chromosomal G-banding dyeing working solution is added dropwise on the drop piece prepared, is covered with dye liquor whole
The individual postdigestive chromosome piece of pancreatin is degree, stands 80 S.
3rd, coloration result
Control dyeing liquor is shown in Fig. 2-3 to the coloration result of chromosome, and figure is all the aobvious band dyes of human chromosome G under 1000 times of visuals field
Color；
Chromosomal G-banding dyeing liquor of the present invention is shown in Fig. 4-5 to the coloration result of chromosome, and figure is all people's dye under 1000 times of visuals field
The aobvious band dyeing of colour solid G.
From Fig. 2, Fig. 3, control dye liquor is ineffective to chromosomal G-banding dyeing liquor, and depth band is unintelligible, so as to shadow
Chromosome observation is rung, causes anaphase analysis difficulty.Even if adjust dye liquor and phosphate buffer（Chromosome B liquid）Ratio
When can not also solve, and be dyed with control Giemsa staining liquid, Color can not also be strengthened by lengthening dyeing time, be dyed
The journey time is grown, and is easily caused dyeing liquor sediment and is attached on chromosome specimen.
From Fig. 4, Fig. 5, when being dyed using chromosomal G-banding dyeing liquor of the present invention, dyeing can be made within a very short time
Body is clear by color, depth color identifying.And to same chromosome specimen, control Giemsa staining liquid dyeing 10-20 effects are still
It is undesirable, dye 80 S with chromosomal G-banding dyeing liquor of the present invention and just can reach good Color.
It can be seen that chromosomal G-banding dyeing liquor of the present invention is good to the Color of human peripheral chromosome, dye liquor of the present invention can
Dyed effective for chromosomal G-banding.
In summary, the dyeing of chromosomal G-banding dyeing liquor of the invention to people's source chromosome is quick, band is clear, and
And it is simple to operate during the chromosomal G-banding dyeing liquor dyeing of the present invention, beneficial to popularization.
Claims (4)
1. efficient chromosomal G-banding dyes liquid kit, it is characterized in that：
Described dyeing liquor A liquid is prepared by the raw material of following proportionings：
Ji Mushi pigment pulvis 2-3 parts by weight, the parts by volume of glycerine 80, the parts by volume of methanol 120；
The described each component of dyeing liquor B liquid is as follows：
The parts by weight of disodium hydrogen phosphate 1.6, the parts by volume of potassium dihydrogen phosphate 9.7, the parts by volume of pure water 1000.
2. dyeing liquor A liquid according to claim 1, it is characterised in that:The methanol is pure to analyze.
3. chromosomal G-banding dyes liquid kit, it is characterized in that：It includes two kinds of mix reagents, and described mix reagent includes：
Dyeing liquor A liquid, dyeing liquor B liquid：
Described dyeing liquor A liquid, dyeing liquor B liquid are the component proportion described in claim 1.
4. chromosomal G-banding dyes liquid kit, it is characterized in that, its application method concretely comprises the following steps：
1）Chromosome specimen is handled with trypsase before dyeing；
2）Chromosomal G-banding dyeing liquor working solution is prepared：The lucky dyeing liquor A liquid 1ml in the kit described in claim 1 are taken,
Dyeing liquor B liquid 5ml in the kit described in claim 1 are taken again, it is mixed by being sufficiently stirred in both the above solution addition staining jar
Close, as chromosomal G-banding dyeing working solution, by step 1）Handled chromosome specimen immerses chromosomal G-banding dyer
Make in liquid；
3）Chromosomal G-banding dyeing condition is as follows：
Room temperature（28-30℃）, dye 72 S-240 S.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610597175.4A CN107664595A (en) | 2016-07-27 | 2016-07-27 | Efficient chromosomal G-banding staining kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610597175.4A CN107664595A (en) | 2016-07-27 | 2016-07-27 | Efficient chromosomal G-banding staining kit |
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| CN107664595A true CN107664595A (en) | 2018-02-06 |
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| CN201610597175.4A Pending CN107664595A (en) | 2016-07-27 | 2016-07-27 | Efficient chromosomal G-banding staining kit |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109540633A (en) * | 2019-01-23 | 2019-03-29 | 北京仁基源医学研究院有限公司 | Karyotyping is dedicated to prepare high resolution chromosome dye liquor |
| CN109540645A (en) * | 2019-01-23 | 2019-03-29 | 北京仁基源医学研究院有限公司 | Karyotyping is dedicated to prepare high resolution chromosome band line desorbed solution |
| CN110658046A (en) * | 2019-10-29 | 2020-01-07 | 广州达安临床检验中心有限公司 | Chromosome C banding method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1183499A (en) * | 1966-12-05 | 1970-03-04 | Baxter Laboratories Inc | Biological Stains |
| CN1434127A (en) * | 2002-01-22 | 2003-08-06 | 裴芳君 | Staining kit and preparation method, staining method, and use thereof |
| CN103323313A (en) * | 2012-03-23 | 2013-09-25 | 黄伏生 | Liquid-based cell analyzer staining method of cells exfoliated from serous cavity by using Wright staining method |
-
2016
- 2016-07-27 CN CN201610597175.4A patent/CN107664595A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1183499A (en) * | 1966-12-05 | 1970-03-04 | Baxter Laboratories Inc | Biological Stains |
| CN1434127A (en) * | 2002-01-22 | 2003-08-06 | 裴芳君 | Staining kit and preparation method, staining method, and use thereof |
| CN103323313A (en) * | 2012-03-23 | 2013-09-25 | 黄伏生 | Liquid-based cell analyzer staining method of cells exfoliated from serous cavity by using Wright staining method |
Non-Patent Citations (2)
| Title |
|---|
| 山东省医学科学院放射医学研究所: "《放射医学检验监测手册》", 30 September 1987 * |
| 王和: "《现代前列腺炎基础与临床》", 31 July 2005 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109540633A (en) * | 2019-01-23 | 2019-03-29 | 北京仁基源医学研究院有限公司 | Karyotyping is dedicated to prepare high resolution chromosome dye liquor |
| CN109540645A (en) * | 2019-01-23 | 2019-03-29 | 北京仁基源医学研究院有限公司 | Karyotyping is dedicated to prepare high resolution chromosome band line desorbed solution |
| CN109540633B (en) * | 2019-01-23 | 2021-10-29 | 北京仁基源医学研究院有限公司 | Special preparation of high-resolution chromosome dye solution for karyotype analysis |
| CN110658046A (en) * | 2019-10-29 | 2020-01-07 | 广州达安临床检验中心有限公司 | Chromosome C banding method |
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