JPS57163352A - Human proinsulin and analogue, manufacture by microbial polypeptide development and conversion to human insulin - Google Patents

Human proinsulin and analogue, manufacture by microbial polypeptide development and conversion to human insulin

Info

Publication number
JPS57163352A
JPS57163352A JP56215948A JP21594881A JPS57163352A JP S57163352 A JPS57163352 A JP S57163352A JP 56215948 A JP56215948 A JP 56215948A JP 21594881 A JP21594881 A JP 21594881A JP S57163352 A JPS57163352 A JP S57163352A
Authority
JP
Japan
Prior art keywords
human
analogue
conversion
manufacture
microbial polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP56215948A
Other languages
English (en)
Japanese (ja)
Inventor
Bui Gooderu Deibuitsudo
Itakura Keiichi
Jii Kuriido Denisu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of JPS57163352A publication Critical patent/JPS57163352A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
JP56215948A 1981-01-02 1981-12-29 Human proinsulin and analogue, manufacture by microbial polypeptide development and conversion to human insulin Pending JPS57163352A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US22204481A 1981-01-02 1981-01-02

Publications (1)

Publication Number Publication Date
JPS57163352A true JPS57163352A (en) 1982-10-07

Family

ID=22830549

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56215948A Pending JPS57163352A (en) 1981-01-02 1981-12-29 Human proinsulin and analogue, manufacture by microbial polypeptide development and conversion to human insulin

Country Status (12)

Country Link
EP (1) EP0055945A3 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JPS57163352A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
KR (1) KR830007828A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (1) AU7914581A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DK (1) DK582481A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ES (1) ES508446A0 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
FI (1) FI814172L (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
GR (1) GR78327B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IL (1) IL64634A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
NZ (1) NZ199391A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
PH (1) PH20660A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ZA (1) ZA818953B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6069029A (ja) * 1983-06-06 1985-04-19 ジエネンテク，インコ−ポレイテツド 組換えｄｎａ技術によるヒトｉｇｆおよびｅｇｆの製造
JPS60500892A (ja) * 1983-03-25 1985-06-20 セルテツク　リミテツド マルチチエインポリペプチドまたは蛋白質およびそれらの製造方法
JPS61221200A (ja) * 1985-03-22 1986-10-01 ノボ　ノルディスク　アクティーゼルスカブ インシュリンの製造方法
JP2003518950A (ja) * 1999-12-29 2003-06-17 ノボ ノルディスク アクティーゼルスカブ 酵母において改良された発酵収率を有するインスリン前駆体及びインスリン前駆体類似体の製造方法
US8129146B2 (en) 1999-12-29 2012-03-06 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
JP2016183118A (ja) * 2015-03-25 2016-10-20 東ソー株式会社 増殖因子前駆体のリフォールディング方法

Families Citing this family (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ199722A (en) * 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
CA1204682A (en) * 1981-06-19 1986-05-20 Saran A. Narang Adaptors, and synthesis and cloning of proinsulin genes
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
JPS58183659A (ja) * 1982-03-31 1983-10-26 ジェネテイックス、インスチチュ−ト、インコ−ポレ−テッド 修飾プロインシュリン前駆体、これを暗号化するｄｎａ配列およびそれらの産生方法
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4758512A (en) * 1984-03-06 1988-07-19 President And Fellows Of Harvard College Hosts and methods for producing recombinant products in high yields
DK58285D0 (da) * 1984-05-30 1985-02-08 Novo Industri As Peptider samt fremstilling og anvendelse deraf
DK437786D0 (da) * 1986-09-12 1986-09-12 Nordisk Gentofte Insulinprecursorer
DE3716722A1 (de) * 1987-05-19 1988-12-01 Hoechst Ag Gentechnologisches verfahren zur herstellung von angiogeninen
US6875589B1 (en) 1988-06-23 2005-04-05 Hoechst Aktiengesellschaft Mini-proinsulin, its preparation and use
US5716927A (en) * 1988-12-23 1998-02-10 Novo Nordisk A/S Insulin analogs having a modified B-chain
US5514646A (en) * 1989-02-09 1996-05-07 Chance; Ronald E. Insulin analogs modified at position 29 of the B chain
WO1990013659A1 (en) * 1989-05-04 1990-11-15 Genentech, Inc. Processes and compositions for the isolation of human relaxin
US5126249A (en) * 1989-05-09 1992-06-30 Eli Lilly And Company Enzymatic removal of a protein amino-terminal sequence
DE59305396D1 (de) 1992-12-02 1997-03-20 Hoechst Ag Verfahren zur Gewinnung von Proinsulin mit korrekt verbundenen Cystinbrücken
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
DE4405179A1 (de) * 1994-02-18 1995-08-24 Hoechst Ag Verfahren zur Gewinnung von Insulin mit korrekt verbundenen Cystinbrücken
KR0150565B1 (ko) * 1995-02-15 1998-08-17 김정재 유전자 조환에 의한 사람 인슐린 전구체의 제조 및 이를 이용한 인슐린의 제조방법
US6068993A (en) * 1997-07-02 2000-05-30 Biobras Sa Vector for expression of heterologous protein and methods for extracting recombinant protein and for purifying isolated recombinant insulin
DE19735711C2 (de) 1997-08-18 2001-04-26 Aventis Pharma Gmbh Verfahren zur Herstellung eines Vorläufers von Insulin oder Insulinderivaten mit korrekt verbundenen Cystinbrücken
KR100253916B1 (ko) * 1997-12-29 2000-05-01 김충환 사람 인슐린 전구체의 제조방법
DK1066328T3 (da) 1998-03-31 2009-02-09 Tonghua Gantech Biotechnology Kimært protein, der indeholder en intramolekylær chaperon-lignende sekvens, og dets anvendelse til insulinproduktion
DE19838097A1 (de) 1998-08-24 2000-03-02 Hoechst Marion Roussel De Gmbh Verfahren zur chromatographischen Reinigung von Insulinen
RU2144957C1 (ru) * 1999-02-26 2000-01-27 Институт биоорганической химии им.М.М.Шемякина и Ю.А.Овчинникова РАН РЕКОМБИНАНТНАЯ ПЛАЗМИДНАЯ ДНК pPINS07, КОДИРУЮЩАЯ ГИБРИДНЫЙ ПОЛИПЕПТИД, СОДЕРЖАЩИЙ ПРОИНСУЛИН ЧЕЛОВЕКА, И ШТАММ БАКТЕРИЙ Escherichia coli - ПРОДУЦЕНТ ГИБРИДНОГО ПОЛИПЕПТИДА, СОДЕРЖАЩЕГО ПРОИНСУЛИН ЧЕЛОВЕКА
JP3406244B2 (ja) 1999-04-30 2003-05-12 伊藤ハム株式会社 新規な融合蛋白質からの組み換えインスリンの製造方法
US6777207B2 (en) 1999-12-29 2004-08-17 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
US6521738B2 (en) 1999-12-29 2003-02-18 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogs
WO2001072323A2 (en) 2000-03-24 2001-10-04 Genentech, Inc. Use of insulin for the treatment of cartilagenous disorders
US6821755B2 (en) * 2000-07-27 2004-11-23 Boehringer Ingelheim International Gmbh Preparation of a recombinant protein in a prokaryotic host cell
AR025646A1 (es) 2000-09-13 2002-12-04 Beta Lab Sa Cepa de levaduras metilotroficas recombinantes productoras de un precursor de insulina, construcciones de adn y metodo para obtener la cepa.
KR100449454B1 (ko) * 2000-10-02 2004-09-21 이현철 단일사슬 인슐린 유도체의 유전자를 포함하는 당뇨병치료용 벡터
US7105314B2 (en) 2001-04-02 2006-09-12 Novo Nordisk A/S Method for making human insulin precursors
WO2002079251A2 (en) * 2001-04-02 2002-10-10 Novo Nordisk A/S Method for making human insulin precursors
EP1592703B1 (en) 2003-01-31 2008-06-04 N.V. Organon Method for protein isolation in anoxic conditions
EP2468881A3 (en) 2005-07-21 2012-08-15 Abbott Laboratories Multiple gene expression including sorf contructs and methods with polyproteins, pro-proteins, and proteolysis
CA2621344C (en) 2005-09-14 2014-12-16 Sanofi-Aventis Deutschland Gmbh Cleavage of precursors of insulins by a variant of trypsin
AU2007298919C1 (en) 2006-09-22 2014-02-06 Novo Nordisk A/S Protease resistant insulin analogues
CN101173006B (zh) * 2006-10-30 2011-12-21 江苏万邦生化医药股份有限公司 一种重组人胰岛素的制备方法
US7790677B2 (en) 2006-12-13 2010-09-07 Elona Biotechnologies Insulin production methods and pro-insulin constructs
US10100098B2 (en) 2006-12-13 2018-10-16 Stelis Biopharma Private Limited Insulin production methods and proinsulin constructs
DK2201120T3 (en) 2007-09-21 2014-03-17 Cadila Healthcare Ltd GCSF fusion protein systems which are suitable for high expression of the peptides
EP2612677B1 (en) 2009-06-26 2015-05-06 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
AU2011343360A1 (en) 2010-12-14 2013-06-06 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and an amino acid
US20130331320A1 (en) 2010-12-14 2013-12-12 Novo Nordisk A/S Fast-acting insulin in combination with long-acting insulin
PL2739646T3 (pl) 2011-05-10 2018-05-30 Stefan Hermann Kompleksowy układ buforowy do renaturacji ludzkiej proinsuliny lub pochodnych proinsuliny
WO2013186138A1 (en) 2012-06-14 2013-12-19 Novo Nordisk A/S Preparation comprising insulin, nicotinamide and arginine
WO2017040363A1 (en) 2015-09-02 2017-03-09 Merck Sharp & Dohme Corp. A process for obtaining insulin with correctly formed disulfide bonds
KR20190036956A (ko) 2017-09-28 2019-04-05 한미약품 주식회사 지속형 단쇄 인슐린 아날로그 및 이의 결합체
CN112584853B (zh) 2018-09-12 2021-12-10 美药星（南京）制药有限公司 一种新型门冬胰岛素原的结构和制备门冬胰岛素的方法
EP4086277A4 (en) 2019-12-30 2024-02-14 Gan & Lee Pharmaceuticals Co., Ltd. LONG-ACTING GLP-1 COMPOUND
KR20220121833A (ko) 2019-12-30 2022-09-01 간 앤 리 파마슈티칼스 컴퍼니, 리미티드 인슐린 유도체
WO2023143458A1 (zh) 2022-01-28 2023-08-03 甘李药业股份有限公司 酰化胰岛素

Family Cites Families (6)

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NL7811042A (nl) * 1977-11-08 1979-05-10 Genentech Inc Werkwijze en middel voor het tot uitdrukking brengen van microbieel polypeptide.
US4411994A (en) * 1978-06-08 1983-10-25 The President And Fellows Of Harvard College Protein synthesis
FI792481A7 (fi) * 1978-08-11 1981-01-01 The Regents Of The Univ Of Enkaroyyttisen proteiinin synteesi mikro-organismia käyttäen.
GR70279B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * 1979-09-12 1982-09-03 Univ California
DE3001928A1 (de) * 1980-01-19 1981-08-06 Hoechst Ag, 6000 Frankfurt Genprodukt eines hoeheren organismus aus einem dieses gen enthaltenden mikroorganismus
CA1200773A (en) * 1980-02-29 1986-02-18 William J. Rutter Expression linkers

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60500892A (ja) * 1983-03-25 1985-06-20 セルテツク　リミテツド マルチチエインポリペプチドまたは蛋白質およびそれらの製造方法
JPS6069029A (ja) * 1983-06-06 1985-04-19 ジエネンテク，インコ−ポレイテツド 組換えｄｎａ技術によるヒトｉｇｆおよびｅｇｆの製造
JPS61221200A (ja) * 1985-03-22 1986-10-01 ノボ　ノルディスク　アクティーゼルスカブ インシュリンの製造方法
JPH06133777A (ja) * 1985-03-22 1994-05-17 Novo Nordisk As インシュリン前駆体をコードするｄｎａ配列
JPH0823985A (ja) * 1985-03-22 1996-01-30 Novo Nordisk As インシュリン前駆体をコードするｄｎａ配列
JP2003518950A (ja) * 1999-12-29 2003-06-17 ノボ ノルディスク アクティーゼルスカブ 酵母において改良された発酵収率を有するインスリン前駆体及びインスリン前駆体類似体の製造方法
US8129146B2 (en) 1999-12-29 2012-03-06 Novo Nordisk A/S Method for making insulin precursors and insulin precursor analogues having improved fermentation yield in yeast
JP2016183118A (ja) * 2015-03-25 2016-10-20 東ソー株式会社 増殖因子前駆体のリフォールディング方法

Also Published As

Publication number Publication date
NZ199391A (en) 1985-12-13
ES8401777A1 (es) 1984-01-01
EP0055945A3 (en) 1982-09-15
ES508446A0 (es) 1984-01-01
FI814172A7 (fi) 1982-07-03
EP0055945A2 (en) 1982-07-14
IL64634A (en) 1986-03-31
IL64634A0 (en) 1982-03-31
GR78327B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1984-09-26
DK582481A (da) 1982-08-13
KR830007828A (ko) 1983-11-07
FI814172L (fi) 1982-07-03
PH20660A (en) 1987-03-23
AU7914581A (en) 1982-07-08
ZA818953B (en) 1983-02-23

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