JPS57127848A - Liquid chromatograph - Google Patents
Liquid chromatographInfo
- Publication number
- JPS57127848A JPS57127848A JP1365781A JP1365781A JPS57127848A JP S57127848 A JPS57127848 A JP S57127848A JP 1365781 A JP1365781 A JP 1365781A JP 1365781 A JP1365781 A JP 1365781A JP S57127848 A JPS57127848 A JP S57127848A
- Authority
- JP
- Japan
- Prior art keywords
- sample
- component
- zone
- column
- effluent side
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/60—Construction of the column
- G01N30/6052—Construction of the column body
- G01N30/6082—Construction of the column body transparent to radiation
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
PURPOSE:To make it possible to detect the fine amount of components, by attaching a light detecting device in the vicinity of the end of the effluent side of a column, and performing the measurement under the state where most of sample components are held by a filler. CONSTITUTION:At the time when the sample is flowed in the column 1, the sample has a form of a single zone. As the column 1 moves, the sample is saparated into a zone of a component A and a zone of a component B. When the zone of the component A reaches the end part of the effluent side and the zone of the component B is still on the half way in the column 1, ultraviolet light is irradiated from an irradiating fiber 8, and the scattered light is detected by a light measuring fiber 9. Then the component A of the sample is measured. When the zone of the component B reaches the end part of the effluent side, the component B of the sample can be measured. Since the measurement is performed under the state where the sample component is supported by the filler, the concentration is not decreased because of the dilution by solvent, and even the very fine amount of the sample can be detected.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1365781A JPS57127848A (en) | 1981-01-30 | 1981-01-30 | Liquid chromatograph |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1365781A JPS57127848A (en) | 1981-01-30 | 1981-01-30 | Liquid chromatograph |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS57127848A true JPS57127848A (en) | 1982-08-09 |
Family
ID=11839271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1365781A Pending JPS57127848A (en) | 1981-01-30 | 1981-01-30 | Liquid chromatograph |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS57127848A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6224129A (en) * | 1985-07-24 | 1987-02-02 | Hitachi Ltd | Concentration analysis method and apparatus |
| EP0211411A2 (en) * | 1985-08-09 | 1987-02-25 | Sato Pharmaceutical Research Institute Limited | Method and apparatus for performing novel high-performance liquid chromatography |
| JPH0261557A (en) * | 1988-08-27 | 1990-03-01 | Hitachi Ltd | Method and apparatus for detecting sample |
| WO2005093410A1 (en) * | 2004-03-29 | 2005-10-06 | Citizen Watch Co., Ltd. | Optical measuring equipment |
| JP2008241546A (en) * | 2007-03-28 | 2008-10-09 | Hitachi High-Technologies Corp | Method and apparatus for confirming removal of moisture in solid-phase extraction column and solid-phase extraction column |
-
1981
- 1981-01-30 JP JP1365781A patent/JPS57127848A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6224129A (en) * | 1985-07-24 | 1987-02-02 | Hitachi Ltd | Concentration analysis method and apparatus |
| JPH0459578B2 (en) * | 1985-07-24 | 1992-09-22 | Hitachi Ltd | |
| EP0211411A2 (en) * | 1985-08-09 | 1987-02-25 | Sato Pharmaceutical Research Institute Limited | Method and apparatus for performing novel high-performance liquid chromatography |
| JPH0261557A (en) * | 1988-08-27 | 1990-03-01 | Hitachi Ltd | Method and apparatus for detecting sample |
| WO2005093410A1 (en) * | 2004-03-29 | 2005-10-06 | Citizen Watch Co., Ltd. | Optical measuring equipment |
| JPWO2005093410A1 (en) * | 2004-03-29 | 2008-02-14 | シチズンホールディングス株式会社 | Optical measuring device |
| US7583382B2 (en) | 2004-03-29 | 2009-09-01 | Citizen Holdings Co., Ltd. | Optical measurement apparatus |
| JP4742031B2 (en) * | 2004-03-29 | 2011-08-10 | シチズンホールディングス株式会社 | Optical measuring device |
| JP2008241546A (en) * | 2007-03-28 | 2008-10-09 | Hitachi High-Technologies Corp | Method and apparatus for confirming removal of moisture in solid-phase extraction column and solid-phase extraction column |
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