CA2127977A1 - Method and apparatus for performing and universally detecting capillary isoelectric focusing without mobilization using concentration gradient imaging systems - Google Patents

Method and apparatus for performing and universally detecting capillary isoelectric focusing without mobilization using concentration gradient imaging systems

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Publication number
CA2127977A1
CA2127977A1 CA002127977A CA2127977A CA2127977A1 CA 2127977 A1 CA2127977 A1 CA 2127977A1 CA 002127977 A CA002127977 A CA 002127977A CA 2127977 A CA2127977 A CA 2127977A CA 2127977 A1 CA2127977 A1 CA 2127977A1
Authority
CA
Canada
Prior art keywords
light beam
light
separations
passage
intensity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002127977A
Other languages
French (fr)
Other versions
CA2127977C (en
Inventor
Janusz B. Pawliszyn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/US1993/000234 external-priority patent/WO1993014389A1/en
Publication of CA2127977A1 publication Critical patent/CA2127977A1/en
Application granted granted Critical
Publication of CA2127977C publication Critical patent/CA2127977C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/05—Flow-through cuvettes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/453—Cells therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Environmental & Geological Engineering (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

The separations resulting from capillary electrophoresis performed in a microbore capillary tube (35) are detected on-line by focusing a light beam (63) in the form of a line or sheet of light (63) on the capillary passage (36) in which the separations (55a-c) take place so that the width of the sheet of light encompasses the length of the passage in which separations of interest are expelled to take plan. The separations form concentration gradients (16) in the capillary passage (36) encompassed by the light beam and cause refraction of portions of the light beam and variations in the intensity of the light beam due to such refraction.
Alternately, the separated components (55a-d) may absorb light of certain frequencies so if the light beam includes light of the certain frequencies, variation in the intensity of the light beam is caused by such absorbance. The variation in the intensity of light along the width of the light beam after passage through the sample (125) is sensed and is indicative of the concentration gradients (160, 161). By using a reagent (125) which reacts with an analyte not having an isoelectric point to form a product having an isoelectric point (100, 101), such analyze may be detected and measured by isoelectric focusing techniques.
CA002127977A 1992-01-13 1993-01-13 Method and apparatus for performing and universally detecting capillary isoelectric focusing without mobilization using concentration gradient imaging systems Expired - Lifetime CA2127977C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US81932592A 1992-01-13 1992-01-13
US819,325 1992-01-13
PCT/US1993/000234 WO1993014389A1 (en) 1992-01-13 1993-01-13 Method and apparatus for performing and universally detecting capillary isoelectric focusing without mobilization using concentration gradient imaging systems

Publications (2)

Publication Number Publication Date
CA2127977A1 true CA2127977A1 (en) 1993-07-22
CA2127977C CA2127977C (en) 2006-03-28

Family

ID=36123268

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002127977A Expired - Lifetime CA2127977C (en) 1992-01-13 1993-01-13 Method and apparatus for performing and universally detecting capillary isoelectric focusing without mobilization using concentration gradient imaging systems

Country Status (1)

Country Link
CA (1) CA2127977C (en)

Also Published As

Publication number Publication date
CA2127977C (en) 2006-03-28

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