JPH11502412A - 狂犬病ウイルスの複製およびその定量的検出のための細胞系ならびに方法 - Google Patents
狂犬病ウイルスの複製およびその定量的検出のための細胞系ならびに方法Info
- Publication number
- JPH11502412A JPH11502412A JP8528827A JP52882796A JPH11502412A JP H11502412 A JPH11502412 A JP H11502412A JP 8528827 A JP8528827 A JP 8528827A JP 52882796 A JP52882796 A JP 52882796A JP H11502412 A JPH11502412 A JP H11502412A
- Authority
- JP
- Japan
- Prior art keywords
- virus
- cell line
- rabies virus
- cells
- cpe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/20011—Rhabdoviridae
- C12N2760/20111—Lyssavirus, e.g. rabies virus
- C12N2760/20151—Methods of production or purification of viral material
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Virology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.名称PH−2(寄託番号:DSM ACC2165)の永久細胞系、またはそれか ら誘導される細胞系。 2.細胞変性効果(CPE)をもつ狂犬病ウイルスを複製する方法において、 a) 請求項1記載の細胞系を複製すべきウイルスで感染させ、 b) 上記細胞をインキュベートし、ついで c) ウイルス粒子が十分高力価に達したのち、ウイルス粒子を単離および精製 する ことからなる方法。 3.工程c)の後にさらに、ウイルス粒子からウイルス抗原を単離し、精製する請 求項2記載の方法。 4.請求項1記載の細胞系内におけるウイルスの複製によって生じる細胞変性効 果に基づいて狂犬病ウイルスを検出する方法において、 a) 上記細胞系を検出すべき狂犬病ウイルスの含有が疑われるサンプルと接触 させ、 b) 上記細胞をインキュベートし、ついで c) インキュベーションの開始後、ウイルス感染によって生じるCPEを顕微 鏡で確認することによってウイルスを確認する ことからなる方法。 5.さらに検出感度が蛍光抗体法(FITC)の場合と比較して約1log10段階 高い請求項4記載の方法。 6.狂犬病ウイルスの複製の阻害剤を検出する方法において、阻害剤の存在下に おける請求項1記載の細胞系内でのウイルスの複製の阻害を、CPEの不存在に よって検出する方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19512057.4 | 1995-03-31 | ||
DE19512057A DE19512057A1 (de) | 1995-03-31 | 1995-03-31 | Zellinie und Verfahren zur Vermehrung von Tollwutviren und deren quantitativen Nachweis |
PCT/EP1996/000424 WO1996030499A1 (de) | 1995-03-31 | 1996-01-30 | Zellinie und verfahren zur vermehrung von tollwutviren und deren quantitativen nachweis |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH11502412A true JPH11502412A (ja) | 1999-03-02 |
JP3632032B2 JP3632032B2 (ja) | 2005-03-23 |
Family
ID=7758407
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP52882796A Expired - Fee Related JP3632032B2 (ja) | 1995-03-31 | 1996-01-30 | 狂犬病ウイルスの複製およびその定量的検出のための細胞系ならびに方法 |
Country Status (14)
Country | Link |
---|---|
US (1) | US5830638A (ja) |
EP (1) | EP0817836B1 (ja) |
JP (1) | JP3632032B2 (ja) |
KR (1) | KR100397247B1 (ja) |
AT (1) | ATE263834T1 (ja) |
AU (1) | AU719795B2 (ja) |
BR (1) | BR9607783A (ja) |
CA (1) | CA2216630C (ja) |
CZ (1) | CZ304597A3 (ja) |
DE (2) | DE19512057A1 (ja) |
ES (1) | ES2218579T3 (ja) |
MX (1) | MX9707444A (ja) |
SK (1) | SK131097A3 (ja) |
WO (1) | WO1996030499A1 (ja) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999035501A1 (en) | 1998-01-09 | 1999-07-15 | The Governing Council Of The University Of Toronto | Presenilin protein interactions |
DE10044648A1 (de) * | 2000-09-08 | 2002-03-21 | Aventis Behring Gmbh | Verfahren zur Vermehrung oder zur Entfernung von Cirocoviren aus biologischem Material |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4664912A (en) * | 1984-10-01 | 1987-05-12 | Wiktor Tadeusz J | Process for the large scale production of rabies vaccine |
-
1995
- 1995-03-31 DE DE19512057A patent/DE19512057A1/de not_active Withdrawn
-
1996
- 1996-01-30 SK SK1310-97A patent/SK131097A3/sk unknown
- 1996-01-30 KR KR1019970706794A patent/KR100397247B1/ko not_active IP Right Cessation
- 1996-01-30 MX MX9707444A patent/MX9707444A/es unknown
- 1996-01-30 CA CA2216630A patent/CA2216630C/en not_active Expired - Fee Related
- 1996-01-30 BR BR9607783A patent/BR9607783A/pt not_active Application Discontinuation
- 1996-01-30 ES ES96901343T patent/ES2218579T3/es not_active Expired - Lifetime
- 1996-01-30 EP EP96901343A patent/EP0817836B1/de not_active Expired - Lifetime
- 1996-01-30 US US08/913,317 patent/US5830638A/en not_active Expired - Lifetime
- 1996-01-30 WO PCT/EP1996/000424 patent/WO1996030499A1/de active IP Right Grant
- 1996-01-30 JP JP52882796A patent/JP3632032B2/ja not_active Expired - Fee Related
- 1996-01-30 CZ CZ973045A patent/CZ304597A3/cs unknown
- 1996-01-30 DE DE59610968T patent/DE59610968D1/de not_active Expired - Lifetime
- 1996-01-30 AU AU45399/96A patent/AU719795B2/en not_active Ceased
- 1996-01-30 AT AT96901343T patent/ATE263834T1/de not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
ES2218579T3 (es) | 2004-11-16 |
DE19512057A1 (de) | 1996-10-02 |
AU719795B2 (en) | 2000-05-18 |
EP0817836A1 (de) | 1998-01-14 |
US5830638A (en) | 1998-11-03 |
KR19980703389A (ko) | 1998-10-15 |
CZ304597A3 (cs) | 1998-01-14 |
KR100397247B1 (ko) | 2003-12-24 |
CA2216630A1 (en) | 1996-10-03 |
ATE263834T1 (de) | 2004-04-15 |
SK131097A3 (en) | 1998-02-04 |
DE59610968D1 (de) | 2004-05-13 |
CA2216630C (en) | 2010-01-26 |
MX9707444A (es) | 1997-12-31 |
EP0817836B1 (de) | 2004-04-07 |
JP3632032B2 (ja) | 2005-03-23 |
AU4539996A (en) | 1996-10-16 |
WO1996030499A1 (de) | 1996-10-03 |
BR9607783A (pt) | 1998-07-07 |
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