JPH1036278A - Agent for suppressing formation of lipid peroxide and composition containing the agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject agent having high free radical capturing and removing action, capable of suppressing the formation of lipid peroxide and having high safety and stability by using an extract of a plant of the family Myrtaceae as an active component. SOLUTION: This agent contains a solvent extract of a plant of the family Myrtaceae. The plant of the family Myrtaceae is preferably those belonging to the genus Callistemon, Dewinia, Eucalyptus, Leptospermum, Melaleuca, Metrosideros, Feijoa, Rhodomyrtus, Syzygium, etc. The extract can be produced by crushing the leaf of a plant of the family Myrtaceae and putting the leaf into an extraction solvent (preferably one or more solvents selected from water, alcohol, acetone and ethyl acetate). A composition having suppressed formation of lipid peroxide can be prepared by using the agent as a component of a composition containing oil and fat such as cosmetics, foods or pharmaceuticals.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a lipid peroxide production inhibitor and a composition containing the same, and more particularly to a lipid peroxide inhibitor comprising at least one solvent extract of a Myrtaceae plant as an active ingredient. It relates to a composition containing the same.

[0002]

2. Description of the Related Art In recent years, it has become clear that free radicals generated in a living body react with unsaturated fatty acids and the like to generate lipid peroxide, which has an adverse effect on the human body. For example,
Lipid peroxide and its oxidative degradation products act on nucleic acids and proteins,
It is known to cause vascular disorders due to arteriosclerosis, hepatic dysfunction, retinopathy, cataract, etc., and to be involved in carcinogenesis and aging.

[0003] In particular, skin, which is directly affected by external environmental factors, is constantly exposed to ultraviolet rays and natural radiation, and by these actions, free radicals are liable to be generated, which causes unsaturated lipids in the biological membrane phospholipids or sebum. Reacts with fatty acids to produce more lipid peroxide. As a result, abnormal pigmentation such as spots and freckles, inflammation, edema, necrosis,
It is easy to cause problems such as wrinkles and aging.

Further, many cosmetics, pharmaceuticals, foods and drinks, etc., contain a large amount of fats and oils, and react with active oxygen during storage or use to generate lipid peroxide, which leads to deterioration in quality and May cause the onset of toxicity to the human body.

[0005] For this reason, search research for drugs that improve abnormalities of lipid peroxide in vivo has been widely conducted. Representative examples include fat-soluble tocopherol (vitamin E) and water-soluble ascorbic acid (vitamin C) as natural products, and BHT as a synthetic compound.
(3,5-tert-butyl-4-hydroxytoluene), BHA (2 (3) -tert-butyl-hydroxyanisole), and the like, but their effects were not satisfactory.

On the other hand, many attempts have been made to obtain a lipid peroxide production inhibitor having a high effect of improving abnormal lipid peroxide in vivo.
629, JP-A-61-24522, JP-A-2-19
3930, JP-A-2-243632, JP-A-2-2-2
64727, JP-A-3-153629, JP-A-3-153
221587, JP-A-4-69343, JP-A-4-6943
Various crude drug extracts are disclosed in, for example, JP-A-202138 and JP-A-4-247010.

[0007]

However, the above crude drug extract is more effective than tocopherol and ascorbic acid.
Although a somewhat high lipid peroxide abnormal improvement effect can be obtained, it does not yet have a sufficient lipid peroxide production inhibitory effect. In addition, the synthetic compounds BHT and BHA have problems such as suspected carcinogenicity. Therefore, there is a demand for a substance that is excellent in the effect of suppressing lipid peroxide production and that is highly safe.

[0008] The production of lipid peroxide can be suppressed by utilizing an antioxidant effect of suppressing oxidation of lipids, an effect of trapping and removing free radicals, and the like. Superoxide dismutase, catalase and the like having an action have extremely low stability and cannot be said to be highly practical. If a stable substance having a free-radical trapping / removing action is obtained, it is expected that a higher lipid peroxide-suppressing action can be obtained in combination with an antioxidant.

The present invention has been made in view of the above viewpoints, and has an object to have a high free radical scavenging and removing effect, an excellent effect of suppressing lipid peroxide production, and a safety and stability. It is an object of the present invention to provide a highly effective lipid peroxide inhibitor and to provide a composition containing the same, which does not easily produce lipid peroxide.

[0010]

Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have intensively searched for a component having a free radical trapping and removing action and a lipid peroxide production suppressing action,
The present inventors have found that a component having a strong free radical scavenging and removing action exists in an extract of a Myrtaceae plant, and completed the present invention.

That is, the present invention is a lipid peroxide production inhibitor containing an extract of a Myrtaceae plant, and a cosmetic, food, pharmaceutical or other composition containing the lipid peroxide production inhibitor.

[0012] Further, the present invention relates to the plant of the family Myrtaceae,
Callistemon, Darwinia, Eucalyptus, Leptospermum, Melaleuca, Metrosideus,
The present invention relates to one or more lipid peroxide inhibitors selected from the group consisting of the genus Feijoa, the genus Albacore, the genus Tenninka, the genus Myrtle, and the myrtle, and a composition comprising the lipid peroxide inhibitor.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail. First, the lipid peroxide production inhibitor of the present invention will be described.

<1> Lipid peroxide production inhibitor of the present invention (1) Extract of Myrtaceae plant The lipid peroxide production inhibitor of the present invention contains an extract of Myrtaceae plant.

First, the plants of the family Myrtaceae used in the present invention include the genus Kallistemon, the genus Darwinia, the genus Eucalyptus, the genus Leptospermum, the genus Melaleuca, the genus Metrosideus, the genus Feijoa, the genus Kinbaika, the genus Tenninka,
Plants belonging to the genera Futomomo, Myrtle, are suitable,
More specifically, Callistemon Speciosus (Callis
emin speciosus: genus Callistemon, Melaleucas squalosa (Melaleuca), Eucalyptus maculata (Eucalyptus maculata), Myrtus communis (Myrtus comm)
unis: Myrtle spp., Feijoa Serowiana (Fejoia), S. aromatikum (Futomomo), Darwinia macrostegia (Darwinia), Rhodomiltus tomentosa (Tenninca), Metrosideros carminea (Metrosideros), Leptospermum -Scopalium (genus Leptospermum) and the like are mentioned as preferable examples. These plants are described in detail in the Horticultural Plant Encyclopedia (Shogakukan Publishing).

The Myrtaceae plant contains a component that has the function of capturing and inactivating free radicals that cause the production of lipid peroxide that is harmful to the human body. What is necessary is just to contain such a component. Therefore, the lipid peroxide inhibitor of the present invention may be one containing the Myrtaceae plant itself by shredding, crushing, pulverizing, etc., but preferably an extract obtained by extracting the component with a solvent is effective. It is contained as a component. The above components can be obtained from any part of the plant body such as leaves, stems, nuts and roots of the family Myrtaceae, but preferably the leaves and stems are used for extraction and the like.

Hereinafter, an example of a method for extracting a component having a free radical scavenging and removing action from a Myrtaceae plant will be described. First, the leaves of the Myrtaceae plant are crushed. At this time, in order to facilitate crushing of the leaves, pretreatment such as drying at a temperature at which the active ingredient does not decompose or freezing using liquid nitrogen may be performed. Next, the pulverized Myrtaceae plants are introduced into the extraction solvent to perform extraction. The time required for extraction is not particularly limited, but suitable conditions may be appropriately determined depending on the treatment state of the plant to be administered to the solvent, the type of the solvent, and the like.

As the solvent used for the extraction, one or more selected from water, alcohol, acetone and ethyl acetate are used. Preferably, a mixed aqueous solution of water and an organic solvent miscible with water is used.
A 9.5% by volume aqueous solution of ethanol or a 20 to 99% by volume aqueous solution of acetone is suitable, and more preferably 5% by volume.
A 0-80% by volume acetone aqueous solution is used.

For example, by extracting with an 80% by volume aqueous solution of acetone at room temperature overnight, an extract of a Myrtaceae plant is obtained. The obtained extract can be used as it is or, if necessary, concentrated to dryness to obtain a solvent extract to be contained in the lipid peroxide inhibitor of the present invention. Also,
If necessary, a component having a free radical trapping / removing action may be further extracted. For example, the dried product is dissolved in a mixture of equal amounts of ethyl acetate and water. Next, this mixed solution is separated into an ethyl acetate layer and an aqueous layer using a separating funnel or the like, and the aqueous layer is concentrated, and a component having a high free radical trapping and removing action is taken out as a dried product.
The dried product thus obtained has a brown color and is water-soluble, but when completely dried, it may be difficult to dissolve in water. It is good to save it in a state.

(2) Lipid Peroxide Production Inhibitor of the Present Invention The lipid peroxide production inhibitor of the present invention contains the above extract of the Myrtaceae plant, and belongs to the family Myrtaceae.
Mixtures of plant extracts of more than one species may be used. Also,
The extract itself may be used, or it may be mixed with various bases, if necessary, together with other lipid peroxide suppressing substances.

The amount and the type of base are not particularly limited, and may be set as appropriate. Examples of other lipid peroxide suppressing substances include conventionally used substances such as tocopherol and ascorbic acid.

Many plants of the family Myrtaceae are used as raw materials for spices, edible fruits, soaps, pharmaceuticals, etc., and many are highly safe.

<2> Composition Containing Lipid Peroxide Inhibitor of the Present Invention The composition of the present invention contains the above-mentioned lipid peroxide inhibitor and contains a lipid that easily produces lipid peroxide. Any product can be applied, and examples thereof include cosmetics, foods, pharmaceuticals, and quasi-drugs.

The cosmetics to which the present invention is applied are not particularly limited in dosage form and use. For example, the dosage form may be any of a solution, an emulsion, a cream, an aqueous gel, and the like. In addition to the base material, there may be mentioned finishing materials such as foundations and control colors. These cosmetics can be manufactured in the same manner as ordinary cosmetics, except that the above-mentioned lipid peroxide production inhibitor is blended.

The amount of the lipid peroxide production inhibitor is not particularly limited, but is preferably in the range of 0.005 to 1% by weight in cosmetics. The cosmetic of the present invention includes various components generally used in cosmetics, that is, an aqueous component, an oily component, a powder component, a surfactant, a humectant, a thickener, a coloring agent, a fragrance, an antioxidant, and a pH adjuster. ,
Chelating agents, preservatives, or ultraviolet protective agents, anti-inflammatory agents, whitening agents, and the like can be added.

When the above-mentioned lipid peroxide production inhibitor is used in foods, the required amount of extract is added to each food material as a general food, and the extract is processed and manufactured by an ordinary manufacturing method. Plants and extracts can be used as they are as health foods or functional foods, or as they are easily eaten. In general, the amount of the above-mentioned lipid peroxide production inhibitor in a food is preferably in the range of 0.01 to 2% by weight.

When the lipid peroxide production inhibitor of the present invention is used for pharmaceuticals, the dosage form is not particularly limited, but it is generally a harmless one or several kinds of vehicles, carriers, excipients, Agents, preservatives, stabilizers, flavoring agents, tablets, granules, capsules, oral preparations such as drench, ointments, creams, external preparations such as drench, sterile solution,
It can be an injection such as a suspension. These can be manufactured using a conventionally known technique.

For example, the above lipid peroxide production inhibitor and an integrating agent such as corn starch and gelatin, an excipient such as microcrystalline cellulose, a leavening agent such as potato starch and sodium alginate, and a sweetener such as lactose and sucrose are distributed. Into powders, tablets, granules and capsules. In the case of an injection, distilled water for injection or polyethylene glycol is used as a solvent, or a dispersant, a buffer, a preservative, an antioxidant and the like may be added as necessary. . When used as an external preparation, petrolatum, paraffin, oils and fats, lanolin and the like are used as bases.

The dosage varies depending on the age of the patient, the type of the disease, the symptoms and the like. In general, in oral administration, the amount of the lipid peroxide production inhibitor is 0.005 to 1% by weight. By using in the range of 0.001 to 1% by weight and 0.005 to 1% by weight as an external preparation,
Expected effects can be expected.

[0030]

Embodiments of the present invention will be described below. The percentages used below are by weight unless otherwise specified.

[0031]

Example 1 Preparation of a Myrtaceae Plant Extract Examples of preparing an extract from a Myrtaceae plant using various solvents are described below. 1g fresh leaves of Callistemon speciosus
Is frozen with liquid nitrogen and then crushed to obtain water, a 20% by volume aqueous ethanol solution, a 50% by volume aqueous ethanol solution, 9
9.5% ethanol, 80% by volume acetone aqueous solution,
Extraction was performed at room temperature for 5 hours using 20 ml of each ethyl acetate solution, and then the same solvent was added to each to make 50 ml.

The extract thus obtained was used as a sample, and the free radical scavenging and removing action was evaluated. This evaluation was performed by stoichiometrically measuring the inactivation of the model radical by each sample (measurement of radical scavenger ability). That is, as a free radical model, a stable free radical, α, α-diphenyl-β
Using picryl hydrazyl (DPPH.), The sample was reacted with the sample at a fixed rate for a certain period of time, and the amount of radicals reduced by the reaction was measured as the decrease in absorbance at 517 nm.

Take 10 μl of each extraction solution into each test tube,
2 ml of 99.5% ethanol, 2 ml of 0.1 M acetate buffer (pH 5.5), 0.5 mM α, α-diphenyl-β-picrylhydrazyl (DPPH.) (Manufactured by Nacalai Tesque, Inc.), Add 1 ml of ethanol solution and add
After 0 minute, the decrease in absorbance at 517 nm was measured. The measurement was carried out in accordance with Kenji Fukuzawa and Junji Terao, "Chemistry and Biology Experiment Line 2, Lipid Peroxidation Experiment Method", p.

Table 1 shows the results. The amount of decrease in absorbance was 0 when the absorbance did not decrease at all, and 0 when the absorbance did not decrease.
The time at which the amount of the free radicals decreased to 1 is represented as 1, and the greater the amount of decrease in the absorbance, the greater the amount of decrease of the free radicals.

[0035]

[Table 1]

From these results, the components having the effect of scavenging and removing free radicals were extracted regardless of the solvent used.
A 0% by volume aqueous acetone solution was found to be preferred.

[0037]

Example 2 Lipid peroxide production inhibitor 10 g of leaves of Callistemon speciosus were frozen in liquid nitrogen, pulverized, and 200 ml of an 80% by volume acetone aqueous solution.
And extracted all day long. The extract was filtered through a Buchner funnel and freeze-dried to obtain 1.11 g of a dried product. This was directly used as a lipid peroxide production inhibitor.

[0038]

Example 3 Lipid Peroxide Production Inhibitor The freeze-dried product obtained in Example 2 was mixed with an equal weight of tocopherol acetate (manufactured by Nacalai Tesque, Inc.) to obtain a lipid peroxide production inhibitor. did.

[0039]

Example 4 Lipid peroxide production inhibitor 10 g of leaves of Callistemon speciosus were frozen with liquid nitrogen and then pulverized, dispersed in 200 ml of an 80% by volume aqueous ethanol solution, and extracted with a Soxhlet extractor for 3 hours. The extract was concentrated to dryness using a rotary evaporator,
0.82 g of a green solid was obtained. This was added to and dissolved in 100 ml of a mixture of equal amounts of water and ethyl acetate.
This mixture was separated using a separating funnel, and the aqueous layer was separated. This aqueous layer was freeze-dried to obtain 0.52 g of a brownish powder, which was used as a lipid peroxide production inhibitor.

[0040]

Example 5 Lipid peroxide production inhibitor Melaleuca squalosa leaves (10 g) were frozen with liquid nitrogen, pulverized, immersed in an 80% by volume acetone aqueous solution (200 ml), and extracted for 5 hours. The extract was filtered through a Buchner funnel and concentrated to dryness using a rotary evaporator to obtain 1.17 g of a green dry product. This was directly used as a lipid peroxide production inhibitor.

[0041]

Example 6 Evaluation of Lipid Peroxide Production Inhibitor of the Present Invention The lipid peroxide production inhibitor of the present invention was evaluated for its free radical trapping and removing effect and lipid peroxide production inhibitory effect. For comparison, d, l-α-tocopherol (manufactured by Nacalai Tesque, Inc.) was similarly evaluated.

The storage stability of the extract of the Myrtaceae plant was also evaluated.

(1) Free radical scavenging and removing action In a 20-ml test tube, 2 ml of a solution prepared by dissolving the lipid peroxide production inhibitor of Example 2 or d, l-α-tocopherol in 99.5% ethanol and 0.1 M Acetate buffer (p
H5.5) Add 2 ml and further add 0.5 ml DDPH.
1 ml of an aqueous ethanol solution is added, and after 30 minutes, 517 ml
The decrease in absorbance was measured. Further, the above experiment was conducted by changing the concentration of the lipid peroxide production inhibitor or d, l-α-tocopherol in various ways.

The results are as shown in FIG. EC indicating the amount required to capture half the amount of DDPH under the above conditions
₅₀ indicates that the lipid peroxide production inhibitor of Example 2 was 19.21 μm.
g, d, l-α-tocopherol was 42.27 μg. This indicates that the free radical scavenging and removing action of the lipid peroxide production inhibitor of Example 2 is about 2.2 times that of d, l-α-tocopherol. Therefore, when the lipid peroxide production inhibitor of Example 2 was used, d, l-α-
Free radicals containing active oxygen can be effectively captured at a lower concentration than tocopherol.

(2) Inhibitory effect of lipid peroxide production In a 20 ml test tube, 2.5% with 99.5% ethanol
Take 2 ml of diluted linoleic acid to 4 ml of 0.05
M phosphate buffer (pH 6.0) was added, and water was further added to 10 ml. To this, the lipid peroxide production inhibitor of Example 2 or d, l-α-tocopherol was added so as to be 0.01% by weight to prepare a sample. Under dark conditions, this is
C., and the amount of lipid peroxide produced by the TBA method was measured over time. In the same manner, the amount of lipid peroxide produced was measured using as a standard no lipid peroxide production inhibitor or d, l-α-tocopherol.

In the TBA method used here, a 2 ml sample was
15% trichloroacetic acid, 0.375 thiobarbituric acid
% Butylhydroxytoluene 0.04%, ethanol 2
Aqueous solution 4 prepared to be 0.25N by volume, hydrochloric acid 0.25N
After immersion in water at 100 ° C. for 30 minutes, immediately cool on ice, centrifuge at 3000 rpm for 10 minutes,
(M. Tiens,
S. D. Aust Biochem. Biophys.
Acta. 1982). The higher the absorbance, the greater the amount of lipid peroxide produced.

FIG. 2 shows the results. In d, l-α-tocopherol, the production of lipid peroxide started after 6 days, whereas in the lipid peroxide production inhibitor of Example 2, 1
Almost no lipid peroxide was produced even after 2 days, indicating that the lipid peroxide production inhibitory effect was excellent.

(3) Inhibitory effect on lipid peroxide production by radical generator AMVN Squalene (Wako Pure Chemical, special grade) was placed in a 20 ml test tube.
Was dissolved in 99.5% ethanol so as to have a concentration of 100 mM, and the lipid peroxide production inhibitor of Example 2 or d, l-α-tocopherol was added in an amount of 0.1 ml.
0.3 ml of an 80% by volume ethanol aqueous solution containing 4 mg was added, and the mixture was stirred with a touch mixer, and then shaken in a 37 ° C. water bath for 1 hour. After 1 hour, AMVN (2,2′-azobis (2,4-
Dimethylvaleronitrile)) 99.5% ethanol (A
After adding 0.5 ml of MVN (5 mM concentration) and stirring with a touch mixer, the mixture was shaken in a 37 ° C. water bath for 18 hours. After shaking, the amount of lipid peroxide produced was measured by the TBA method. In addition, a sample without addition of a lipid peroxide inhibitor or d, l-α-tocopherol was used as a standard, and a sample without squalene oxidized was used as a blank.
The amount of lipid peroxide produced was measured by Method A.

The results are shown in terms of the oxidation inhibition rate where the absorbance of the standard is 0% and the absorbance of the blank is 100%.
The results are shown in FIG. The lipid peroxide production inhibitor of Example 2 stops the radical chain reaction induced by the radical generator AMVN by eliminating radicals,
It can be seen that lipid peroxidation is effectively suppressed. Its potency was 1.34 times that of d, l-α-tocopherol.

(4) Storage stability 1 g of Callistemon speciosus leaves was extracted with an 80% by volume aqueous ethanol solution, and this extract was left at room temperature without concentration, and after 2 weeks and 4 weeks, DPPH radicals were removed. The effect of scavenging and removing active oxygen was examined by using this method. As a result, almost no difference was observed between the measured value immediately after extraction and the measured values after 2 weeks and 4 weeks, and it was recognized that the active oxygen scavenging and removing action was not reduced. After 4 weeks, the formation of a precipitate was slightly observed.

Next, examples of the composition containing the lipid peroxide production inhibitor of the present invention will be described.

[0052]

Example 7 Cosmetics (cream) The components A and B in Table 2 were each heated and dissolved at 70 ° C.
While stirring the component A, the component B was gradually added thereto, and the emulsion was inverted. After cooling to 35 ° C., the component C was added to obtain a cream.

[0053]

[Table 2]

[0054]

Examples 8 and 9 Cosmetics (cream) The components A and B in Table 3 were each heated to 70 ° C. and dissolved. While stirring the component A, the component B was gradually added thereto, and the mixture was emulsified and inverted, and then cooled to 35 ° C. to obtain a cream.

[0055]

[Table 3]

[0056]

Comparative Example 1 Cosmetics (cream) In Table 3, d, l-δ-tocopherol was replaced by 0.05 in place of the lipid peroxide production inhibitor of Example 2 or Example 3.
A cream in which the weight% was blended was obtained as Comparative Example 1.

[0057]

Example 10 Lipid Peroxide Generation Inhibiting Effect of Cream Containing Lipid Peroxide of the Present Invention Squalene (manufactured by Wako Pure Chemical Industries, special grade), 200 μm of 20% by volume chloroform solution on 4 cm filter paper (manufactured by Kiriyama, No. 5c)
1 was uniformly impregnated and dried. After drying, the cream of Example 8, Example 9 or the cream 12 of Comparative Example 1
0 mg is uniformly applied to the filter paper, and irradiated with ultraviolet light (FL20S-BLB lamp manufactured by Toshiba, irradiation intensity 3.2 VW / c)
m ² ) for 5 hours. After irradiation, the lipid contained in the filter paper was subjected to Soxhlet extraction with 99.5% ethanol for 1 hour, and then the amount of lipid peroxide was measured by the TBA method. Similarly,
The lipid peroxide inhibitor or d, l-α-tocopherol was not added as a standard, and no cream was applied and no UV irradiation was used as a blank. It was measured.

The results are shown in terms of the oxidation inhibition rate where the absorbance of the standard is 0% and the absorbance of the blank (when squalene is not oxidized) is 100%. FIG. 4 shows the results. As is clear from these results, it was found that the lipid peroxide production inhibitor of the present invention sufficiently suppressed the production of lipid peroxide due to ultraviolet rays even in creams. Its efficacy was superior to tocopherol, which is now commonly used in cosmetics. In addition, it was found that when tocopherol was used in combination with the extract of the family Myrtaceae, higher effects could be obtained.

[0059]

Example 11 Cosmetic (Lotion) The components shown in Table 4 were homogeneously mixed and dissolved at room temperature to obtain a lotion.

[0060]

[Table 4]

[0061]

Example 12 Food (Cookies) Wheat flour containing 2% by weight of the lipid peroxide production inhibitor of Example 2 was seasoned with salt, sucrose, butter, etc., and stirred well with an appropriate amount of water. Bake at ℃ for 30 minutes to make cookies.

[0062]

Example 13 Food (Jelly) 12 g of agar was dissolved in 1 L of water with stirring and heated.
g, 150 g of starch syrup and a little salt were added, and dissolved by heating with stirring. After adding 2% by weight of the lipid peroxide production inhibitor of Example 2 and an appropriate amount of flavor, the mixture was cooled to jelly.

[0063]

Example 14 Pharmaceuticals (tablets and capsules) The components shown in Table 5 were uniformly mixed in the following parts by weight to obtain tablets or capsules.

[0064]

[Table 5]

[0065]

Example 15 Pharmaceutical (powder, granule) The components shown in Table 6 were uniformly mixed in the following parts by weight to obtain a powder or granule.

[0066]

[Table 6]

[0067]

Industrial Applicability The lipid peroxide production inhibitor of the present invention contains an extract of a Myrtaceae plant as an active ingredient, and has a high free radical scavenging and removing effect. As a result, it is excellent in lipid peroxide production inhibitory effect and stable. High safety and safety.

Further, the lipid peroxide production inhibitor of the present invention is
By containing the composition in a composition containing fats and oils such as cosmetics, foods, medicines, and quasi-drugs, a composition in which generation of lipid peroxide is suppressed can be obtained.

[Brief description of the drawings]

FIG. 1 is a graph showing the amount of free radicals reduced by an extract of a Myrtaceae plant or d, l-α-tocopherol.

FIG. 2 is a graph showing the change over time in the amount of lipid peroxide produced in the presence of an extract of a Myrtaceae plant or d, l-α-tocopherol.

FIG. 3. In the presence of a fat-soluble radical generator AMVN,
The figure which shows the oxidation inhibition rate of a lipid by the extract of a Myrtaceae plant, or d, l- (alpha) -tocopherol.

FIG. 4 is a graph showing the inhibition of lipid oxidation by a cream containing an extract of a Myrtaceae plant, d, l-α-tocopherol or a mixture thereof under ultraviolet irradiation.

Continued on the front page (51) Int.Cl. ⁶ Identification code Agency reference number FI Technical indication A61K 7/48 A61K 7/48 (72) Inventor Masaki Okada 27-1, Takashimadai, Kanagawa-ku, Yokohama-shi, Kanagawa Yokohama Co., Ltd.

Claims (4)

[Claims] 1. A lipid peroxide inhibitor containing a solvent extract of a Myrtaceae plant. 2. The plant of the family Myrtaceae is of the genus Callistemon,
The peroxidation according to claim 1, wherein the peroxidation is at least one selected from the genus Darwinia, Eucalyptus, Leptospermum, Melaleuca, Metrosideus, Feijoa, Kinbika, Tenninka, Futomomo, Myrtle. Lipid inhibitors. 3. An extraction solvent comprising water, alcohol, acetone,
3. The lipid peroxide inhibitor according to claim 1, which is at least one member selected from the group consisting of ethyl acetate. 4. A composition containing the lipid peroxide inhibitor according to claim 1.