JPH10262452A - Single stem training graft nursery plant of tomato - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a single stem training graft nursery plant of a tomato which does not need much labor for managing rearing though the sizes of its leaves and the thickness of its stems of side branches are not inferior to those of 'normal single stem training graft nursery plant'. SOLUTION: A graft nursery plant A with two cotyledons 1a, 1b and a first true leaf 2a is cut respectively at a point between the cotyledons and the first true leaf and a point under the cotyledons to divide into the three parts of an upper stage scion part A-1 with the first true leaf 2a, a middle-stage scion part A-2 with the two cotyledons and a lower-stage core picking seedling A-3 to graft the part A1 to a rootstock seedling B-1. Then the part A-2 is graft to another root stock B-2 and then at the same time of, before or after the graft, one of the two cotyledons 1a and 1b at the part A-2 is removed from its root part to extend a side branch 5a from the root of the residual cotyledon to obtain the single stem training graft nursery plant of the tomato.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an improved grafted tomato seedling of tomato, and in particular, compared to "ordinary one-tailored grafted seedling", the size of leaves and the thickness of the side branch stem are comparable. The growth of the plant height is also good, the growth is progressing smoothly, the appearance of the grass is favorable, there is no concern that consumers will feel deformed seedlings, and it is not necessary to grow earlings until the large seedling stage of 4 to 5 true leaves The present invention relates to a grafted tomato seedling that does not require labor for raising seedlings and the like, and does not require both rootstock seedlings to be grown according to the thickness of the stalks of the scion.

[0002]

2. Description of the Related Art Conventionally, as shown in FIG. 3, a cotyledon (A) of a scion seedling (A) having two cotyledons (1a, 1b) and a first leaf (2a) and a first leaf of a true leaf. The center of (2a) is pinched (cut) to obtain a scion (A-) having the first true leaf (2a).
1) and a seedling having two cotyledons (1a, 1b), and the scion (A-1) was grafted to the rootstock seedling (B-1). To promote the growth of the side branches (5a and 5b) from the base of the cotyledon,
After about a week, the seedling having about 2 leaves on the side branches is cut diagonally at a position of 1 to 2 cm immediately below the cotyledon, and a scion (A-2)
And the second seeding seedling, the scion (A-2) is grafted to another rootstock seedling (B-2), and side branches (5a, 5a, 5b) is stretched, and two side branch tailored grafted seedlings (A-2 and B-2 grafted seedlings together with "one main branch grafted seedling of normal tomato (grafted seedling of A-1 and B-1)") ) Are known (hereinafter, referred to as “two grafted seedling grafting method”) (“agriculture and horticulture”,
Vol. 71, No. 4, 1996, p. 490). On the other hand, as shown in FIG. 4, two cotyledons (1a, 1
b), the first true leaf (2a) and the second true leaf (2b) of the hogi seedling (A) having the true first leaf (2a) and the second true leaf (2b). And the immediate lower part of the cotyledon, respectively, to cut the scion containing the second true leaf (2b) in the upper part, the first true leaf (2a) and the two cotyledons (1a, 1b) in the middle part. A method of obtaining three grafted seedlings, and a lower part of the seedling, and cutting the upper part and the middle part of the seedlings to rootstock seedlings, respectively, to obtain one tailored grafted seedling of tomato (hereinafter referred to as " 2
A single-seed seedling grafting method by simultaneous production of strains ") is known (see pages 490 to 491).

[0003] However, the two-sided grafted seedling of tomato obtained in the "two-tailed seedling grafting method" is the side branch 2
Because the book grows at the same time, "Normal one-tailored grafted seedling"
As compared with, the leaves are smaller and the stems of the side branches are thinner, the growth of the plant is inferior, and the growth of grafted seedlings is delayed. Also, tomato seedlings that are usually sold are tailored seedlings with one main branch, and the grafted seedlings with two side branches are not preferable in appearance of grass, and there is a concern that consumers may feel that they are deformed seedlings. Also, over a long period of about two weeks, it takes time and effort to control seedlings, such as watering and temperature control, and fertilizer management such as application of liquid fertilizer. The seedling raising efficiency becomes poor.

[0004] On the other hand, the "single tailoring seedling grafting method by simultaneous production of two strains" is a method in which an ear tree seedling is divided into four true leaves including a first leaf and a second leaf.
Up to five large seedlings have to be raised, so it takes time for nursery management and the like. Also, the thin stem directly above the first leaf of the true leaf of the large seedling and the thick stem just below the cotyledon are cut, so the rootstock seedling Must be grown according to the thickness of each stem. In addition, since the seedlings are raised until the seedlings are turned into large seedlings, the seedling raising tray needs to have a large planting hole, and the efficiency of the seedling raising deteriorates.

[0005]

SUMMARY OF THE INVENTION The present invention relates to a "normal 1
Compared with `` tailored grafted seedlings '', the size of leaves and the thickness of side branch stems are comparable, the growth of plant height is good, the growth progresses smoothly, the appearance of the grass is favorable, the consumers are deformed seedlings There is no need to feel that the seedlings need to be raised until the 4 to 5 leaves of the large seedlings, no need to manage the seedlings, etc. The purpose is to obtain a grafted seedling of tomato without the hassle that must be raised.

[0006]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies to solve the above-mentioned problems, and as a result, have obtained two or three true leaves having two cotyledons and true leaves. In the middle of the cotyledon and the first leaf of the true leaf and immediately below the cotyledon,
Each part is divided into three parts: an upper spikelet having true leaves, a middle spikelet having two cotyledons, and a pinned seedling, and the upper spikelet is grafted to a rootstock seedling on a nursery tray in a conventional manner. At the same time, the middle step scions are grafted to the rootstock seedlings of the same growth period in another seedling tray, and either of the two cotyledons of the middle step scions during grafting, before grafting, or after grafting. When one was removed from its root and the side branch was extended from the root of the remaining cotyledon, it was found that the above object could be achieved, and the present invention was completed based on this finding. That is, the present invention relates to a scion seedling (A) having two cotyledons (1a, 1b) and a first true leaf (2a) between the cotyledon and the first true leaf and below the cotyledon, respectively. After cutting, the upper scion part (A-1) having the first leaves (2a) and the middle scion part (A-) having two cotyledons are cut.
2) and the lower section of the cored seedling (A-3), the upper scion part (A-1) was grafted to the rootstock seedling (B-1), and the middle scion part (A-2). ) Is grafted to another rootstock seedling (B-2), and two cotyledons (1a, 1b) of the middle scion part (A-2) at the same time as, before, or after grafting.
Is a single-seed grafted seedling of tomato obtained by removing any one of the roots from its root and extending the side branch (5a) from the root of the remaining cotyledon.

Hereinafter, a method of preparing a single grafted seedling of tomato of the present invention will be described with reference to the accompanying drawings. FIG.
FIG. 2 is a schematic explanatory view showing a method for producing a single tailored grafted seedling of tomato of the present invention, and FIG. 2 is a schematic explanatory view showing another production method of the present invention.

In FIG. 1A, two cotyledons 1a,
1b and the middle of the first leaf 2a (cutting line 3) and the lower side of the cotyledon (cutting line 4) of the scion seedling A having the first leaf 2a are cut off as shown in FIG. As shown in (b), the upper scion part (A-1) having the first true leaves (2a), the middle scion part (A-2) having two cotyledons, and the lower pinning seedling (A-)
3), and the upper scion part (A-1) was grafted to the rootstock seedling (B-1) as shown in (c) of FIG. As shown in FIG. 1, a “normal single tailored grafted seedling” was obtained, and as shown in FIG.
2) is grafted to another rootstock seedling (B-2) and, at the same time as, before, or after the grafting, the middle stepped part (A-
2) One of the two cotyledons (1a, 1b) is removed from its root, and the side branch (5a) is extended from the root of the remaining cotyledons as shown in FIG. As shown in FIG. 1 (g), a single grafted seedling of the tomato of the present invention is obtained.

FIG. 2 is a schematic explanatory view of an embodiment showing another embodiment of the present invention. That is, as shown in FIG. 2 (h), the scion A is cut along the cutting line 3, and the upper scion part (A-1) each having a true leaf and the two cotyledons (1)
a) and two cotyledons obtained by raising seedlings (cultivation) for a suitable period in the form of seeded seedlings as shown in FIG. The middle scion part (A-2) having a part is divided into two parts in the axial direction as shown in FIG.
-2a) and a scion (A-2b) having one cotyledon 1b are obtained, and these scions are respectively divided into two rootstock seedlings (B-3,
B-2), grafted from the root of the cotyledon to the side branch 5a,
5b is expanded to obtain a single grafted seedling of the tomato of the present invention.

In the present invention, simultaneously with grafting, before grafting,
Alternatively, it is extremely important to remove one of the two cotyledons (1a, 1b) of the middle scion part (A-2) from the root part after grafting.

That is, when one of the cotyledons is not removed, as described above, two side branches grow simultaneously from the vicinity of the root of the cotyledon, and the leaves are compared with the “normal one-tailed grafted seedling”. It becomes small and the stem of the side branch becomes thin, so that the growth of the plant height is poor and the growth of grafted seedlings is delayed. In addition, normally sold tomato seedlings are tailored seedlings with one main branch, and the grafted seedlings with two side branches have a disadvantage that the appearance of grass is unfavorable and consumers may feel that they are deformed seedlings. It is not preferable.

As shown in FIG. 1 (b), one of the two cotyledons (1a, 1b) is removed from the root of the cotyledons (1a, 1b) and the main cotyledon (1a, 1b). This can be performed at the same time as when the middle of the first leaf is cut by the cutting line 3, or before, or after the grafting.

A specific example of “before grafting” is a cutting line 3
By cutting, each of which has a true leaf
1 and two parts of the seeded seedling having two cotyledons 1a and 1b. The seeded seedling is continuously raised in the form of a seeded seedling (cultivation) for several days, and then one of the two cotyledons. Is removed from the root (if there is a side branch from the root of the cotyledon, this side branch is also removed), and the lower stem of the cotyledon of the trained pinched seedling is cut along a cutting line 4 so as to cut one side. The middle scion part A-2 having the cotyledon 1a is obtained and grafted to the rootstock seedling B-2.

As the scion seedlings used in the present invention, seedlings that have been arbitrarily fertilized can be used, but those in the seedling stage are preferred. In other words, in the case where the seedlings are grown up to 4 to 5 true leaves, larger culture soil and larger trays for planting holes are required for raising the seedlings, and fertilization management such as irrigation, temperature and humidity, and sunlight. In the case of large seedlings, rootstock seedlings also have the disadvantage that they must be fertilized and managed for a long period of time to fit the stems of the scion. Pruning seedlings belonging to the seedling stage of 2-3 leaves are preferred. Specifically, two cotyledons and 2-3
The stem diameter near the cotyledon where one true leaf has developed is 1.5 to 2.5 m
m. Seedlings at the seedling stage are preferred.

Next, when the upper scion part (A-1) is grafted to the stock (B-1), the stock of the stock will have substantially the same cross-sectional shape as to be joined to the scion. It is preferable that the rootstock seedlings have almost the same or almost the same growth period as the spikelets.

After grafting in this way, curing is carried out in accordance with the usual method of growing grafted seedlings to obtain one-seated grafted seedlings of tomato. The training is preferably performed for 3 to 4 days under various conditions such as a temperature of 25 to 30 ° C., a humidity of 90% or more, a cloudy day of sunshine, and no wind.

During this curing period, a side branch starts to be generated from the root of the cotyledon 1a or 1b from the root of the cotyledon of the grafted seedling, and can be extended. Thus, a single grafted seedling of the tomato of the present invention can be obtained.

In the middle of the above-mentioned seedling stage, the seedlings grown from a seedling tray of 128 to 200 holes (not shown for simplicity of the drawing) during the single-leaf stage of the spikelet seedlings are grown. During the growing season, the cucumber mosaic virus attenuated virus (C
MV) The particles are diluted in a phosphate buffer so as to have a concentration of 50 to 100 μg / ml, and sprayed by a roller method (JP-A-4-330).
005), or when inoculated by the sprayer method,
It is preferable because disease-resistant tomato seedlings can be easily obtained. 1 and 2, reference numeral 8 denotes a sprayer, and reference numeral 9 denotes a suspension of attenuated cucumber mosaic virus. In this case, it is preferable that the number of holes in the seedling raising tray is large in order to efficiently use expensive virus particles. 1 to 4, reference numeral 7 denotes a graft support. As the support used here, any support such as a clip type, a tube type, an adhesive, and a pin can be adopted.

Since the grafted seedling of the present invention is grafted with a cored scion, the initial growth immediately after grafting is slightly delayed as compared with a normal single tailored grafted seedling. Or the growth and elongation of the side branch of the root of 1b are advanced, and the grafted seedling of the present invention is no less inferior in the plant height, the number of leaves, etc. than the normal one-tailed grafted seedling, and the flowering position of the first stage is also almost Will be the same.

Hereinafter, the effects of the present invention will be described more specifically with reference to cultivation test examples of single-seed grafted tomato seedlings obtained by the method of the present invention.

[0021]

Example 1 (1) Raising of seedlings of seedlings 200-hole tray “Yanmar vegetable tray 25-200 (cell size, 25 mm square × depth 45) manufactured by Yanmar Agricultural Machinery Co., Ltd.
mm) ”is filled with Sakata Seed's cultivation soil“ Supermix A ”, and seeds of tomato scion seedlings“ Summerkiss (Nippon Del Monte) ”are sown in this hole, and under appropriate lighting conditions Under proper temperature and moisture conditions, seedlings were bred for 25 days according to a conventional method to obtain scion seedlings belonging to the seedling stage suitable for grafting. Table 1 shows the growth status of the seedlings.

[0022]

[Table 1]

From the results shown in Table 1, the thickness of the stem directly above and below the cotyledon of the hogi seedlings is almost the same. When two scions are joined to each other, the sowing time of the rootstock used for both scions can be the same.

(2) Preparation of Grafted Seedlings by the Method of the Present Invention Two or three true leaves having two cotyledons (1a, 1b) and the first true leaves (2a) belong to the seedling stage (A). ) Is cut between the cotyledon and the first leaf of the true leaf and the lower side of the cotyledon, respectively, so that the upper stalk (A-) having the first leaf of the true leaf (2a) is cut.
1) Divided into three parts, a middle scion part (A-2) having two cotyledons and a lower pinning seedling (A-3), and the upper scion part (A-1) was used as a rootstock seedling (B) -1) was grafted to obtain a grafted seedling of one main branch of ordinary tomato. In the middle of the above seedling stage, a single seedling of a true leaf of a scion seedling was inoculated with a cucumber mosaic virus attenuated virus ("NDM-1", manufactured by Nippon Del Monte Co., Ltd.) by a spray roller method. On the other hand, middle section Hogi (A-2)
Is grafted to another rootstock seedling (B-2), and at the same time as the grafting, two cotyledons (1a, 1b) of the middle stalk (A-2)
One (1b) was removed from its root, and the side branch (5a) was extended from the root of the remaining cotyledons (1a) to obtain a grafted seedling (one tomato tailored grafted tomato) according to the method of the present invention. When grafting, match the stalks of the scion with the stalks of the cut rootstock, and use the grafting support super-wiz (Nasnicks).
It joined using (7). The rootstock seedling used in the method of growing a scion seedling described above is “Yanmar vegetable tray 25-200 (cell size, 25 mm square × depth 4).
5mm) "instead of" Yanmar Vegetable Tray 30-12
8 (cell size, 30 mm square x 45 mm depth) "and tomato rootstock seedlings," Tomato rootstock seedlings, "Kagemusha (Takii Seedlings)" Except for using it, it was obtained by growing in the same manner.

[0025]

[Comparative example]

Preparation of Grafted Seedlings (Two Side Branched Grafted Seedlings) According to Comparative Example (1) Growing Hogi Seedlings Used for Comparative Example Nasnicks 72-hole tray “Wiztre-72”
(Size of cell hole, 40 mm square x 57 mm depth) "is filled with the cultivation soil" Supermix A "manufactured by Sakata Seed Co., Ltd. Under the appropriate lighting conditions, while appropriately maintaining the temperature and moisture, and raising the seedlings for 25 days by an ordinary method to obtain scion seedlings belonging to the seedling stage suitable for grafting. FIG.
As described in the above section, the middle of the cotyledon and the first leaf (2a) of the hogi seedling (A) having two cotyledons (1a, 1b) and the first leaf (2a) is cut off (cut). ), And a scion (A-1) having a first leaf (2a) and two cotyledons (1a,
1b), the scion (A-1) is grafted to the rootstock seedling (B-1), and the remaining seeded seedlings are irrigated with a thin liquid fertilizer. The growth of the side branch is promoted, and about 2 weeks after the core removal, the seedling having about 2 leaves on the side branch is cut diagonally at a position of 1 to 2 cm immediately below the cotyledon, and a scion (A-2). Divided into a second seeding seedling, the scion (A-
2) grafted to another rootstock seedling (B-2) different from the above,
The side branches (5a, 5b) were extended from the roots of the cotyledons, respectively.
And B-1 grafted seedlings) along with two side branch tailored grafted seedlings (A-
2 and B-2) (Comparative Example) were obtained. In the middle of the seedling stage, the cucumber mosaic virus attenuated virus (Nippon Del Monte,
"NDM-1") was inoculated by the spray roller method.

[0026]

[Control example]

Preparation example of grafting seedlings with one main branch of normal tomato. An upper stage having the first true leaf by cutting between the cotyledon and the first true leaf of the spikelet seedling belonging to two to three true leaves having the two true cotyledons and the first true leaf The cuttings were divided into two parts, a scion part and a cored seedling, and the scion part was grafted to a rootstock seedling to obtain a grafted seedling with one main branch of ordinary tomato. In the middle of the seedling stage, the cucumber mosaic virus attenuated virus (manufactured by Nippon Del Monte, "NDM-
1)) was inoculated by the spray roller method.

The results obtained in the methods for preparing grafted seedlings according to the present invention, comparative examples and control examples are shown in Tables 2, 3, 4, 5 and 6, respectively.

[0028]

[Table 2]

Table 2 shows the required sizes of the trays, the amount of soil to be cultivated, the number of spikelets obtained per tray hole, and the spikelets obtained per tray in the methods for preparing grafted seedlings according to the present invention, comparative examples and control examples. The total number was examined. From the results in Table 2, the method of the comparative example shows that the number of holes per predetermined area of the tray is small (72 holes) and the tray having a large hole (cell) volume is related to the growth of the seedlings for a long period of 40 days. , And a lot of soil must be used. Therefore, the total number of scions obtained per tray having a predetermined area is as small as 144. In general, in both the seedling stage of 2.5 true leaves and the large seedling stage of 4.0 true leaves, the diameter of the stem directly above the first leaf of the true leaf and the diameter of the stem immediately below the cotyledon are approximately 1 mm. There is a difference, and when these are brought into contact with two rootstock seedlings, respectively, rootstock seedlings having almost the same growing season cannot be used. The fact that the stems of the scion and the rootstock have the same thickness leads to a high joining rate, so that the method of the comparative example has a risk of decreasing the joining rate. On the other hand, in the control method, the seedlings are raised for a relatively short period of 25 days, so that there are many holes per predetermined area (200
Hole), seedlings can be raised with a small amount of soil using a tray with a small cell volume, but the total number of scions obtained per tray of a predetermined area is as small as 200. On the other hand, the present invention has the characteristic that the seedlings are grown for a relatively short period of 25 days, so that the seedlings can be grown with a small amount of soil using a tray having a large number of holes per predetermined area (200 holes) and a small cell volume. In addition, two scions per tray hole can be obtained, so that the same size of the tray as the control and the amount of soil cultivation, the scion twice as large as the control, that is, 40 times
It has the advantage that zero spikes can be obtained. In addition, it can be seen that fertilization management in the seedling stage can be shortened by 10 days to 2 weeks compared to the large seedling stage, a small amount of culture soil is required, and irrigation and the volume of the seedling raising tray can be reduced. In the method of the present invention, the diameter of the stem directly above the cotyledon and the diameter of the stem immediately below the cotyledon are almost the same. Since these are respectively in contact with two rootstock seedlings, rootstock seedlings having almost the same growth period can be used, and it is easy to achieve a joining rate of 100%. It also has the feature that a graft support of the same standard can be used.

[0030]

[Table 3]

Table 3 shows the tray sizes required for raising seedlings and rootstocks and the number of days required from sowing to grafting in the methods of preparing grafted seedlings according to the present invention, comparative examples and control examples. It is. In the method of Comparative Example, seedlings were raised for 25 days,
The middle of the cotyledon and the first true leaf (2a) of the scion seedling (A) having two cotyledons (1a, 1b) and the first true leaf (2a) is cut off and cut into a true leaf. The scion (A-1) having the first leaf (2a) and the pinning seedling having two cotyledons (1a, 1b) are divided and the scion (A-1) is replaced by a rootstock (B-1) On the other hand, the remaining seeded seedlings were irrigated with thin liquid fertilizer to promote the growth of side branches from the roots of the cotyledons, and two weeks after seeding, the number of leaves of the side branches became about two, 1-2 just below cotyledon
cm and cut back diagonally, grafting again, so that both the scion and the rootstock grow into large seedlings. Therefore, a tray with a small number of holes per predetermined area of the tray (72 holes) and a large hole (cell) volume is required. Must be used, and a large amount of grafted seedlings cannot be obtained in a short period of time. That is, it has a disadvantage of low efficiency. The control method also has the disadvantage of being inefficient. On the other hand, it can be seen that the present invention is efficient because a large amount of grafted seedlings can be obtained in a short period of time.

[0032]

[Table 4]

Table 4 shows that the grafted seedlings obtained in the trays were placed in a box, covered with vinyl, sealed, sealed, and kept in a humid condition at room temperature of 28 ° C. in the method for preparing grafted seedlings according to the present invention and the comparative example. The results are shown in Fig. 2. The seedlings were stored in a germinator diamond green center H-8 (manufactured by Keiku Seisakusho Co., Ltd.) for a day, then moved to a plastic greenhouse, and the growth status of seedlings 55 days after sowing was investigated. From the results shown in Table 4, the grafted seedlings of the present invention grew 20%, 60%, and 20% of the plant height, the length of the side branch, and the stem diameter of the base of the side branch, respectively, as compared with the comparative example, and grew vigorously. You can see that. That is, it can be seen that tomato-grafted seedlings can be obtained in a short time.

[0034]

Example 4 Table 5 shows the growth results of grafts obtained by the present invention and comparative examples 20 days after grafting. This Table 5
Therefore, although the grafted seedling of the present invention grows more slowly than the control grafted seedling, it grows up to 3 days inside and outside with a plant height of about 9 cm at about 20 days after grafting and can be shipped as a cell tray grafted seedling. It can be seen that the size has reached.

Next, the above seedlings were raised in pots (12 cm polypots) and raised, and the results of a growth comparison survey conducted 54 days after grafting are shown in Table 6.

[0036]

[Table 6]

From the results in Table 6, it can be seen that the grafted seedlings of the present invention after 54 days of grafting were slightly inferior in growth to the control grafted seedlings, but were not so inferior. It turns out that there is not much difference.

[0038]

[Effects of the present invention] The present invention provides a "normal one-tailored grafting seedling".
Compared to, the size of the leaves, the thickness of the stem of the side branch is not inferior,
The growth of the plant height is also good, the growth is progressing smoothly, the appearance of the grass is favorable, there is no concern that consumers will feel deformed seedlings, and it is not necessary to grow earlings until the large seedling stage of 4 to 5 true leaves In addition, it is possible to obtain a grafted tomato seedling that does not require labor for raising seedlings and the like, and does not require both rootstock seedlings to grow according to the thickness of the stalk of the scion. Further, as described above, the present invention can be used for grafting by cutting again the stems and leaves of the scion that has been discarded in the conventional grafting operation. And
Almost at the same time as the conventional grafting, the remaining foliage of the scion seedlings can be cut and used for grafting, so that the effect of mass production of grafted seedlings can be obtained. In addition, it is possible to provide consumers with tomato seedlings that have almost the same appearance as grass that are usually dealt with. In addition, attenuated CMV inoculated seedlings using a special technique can be divided and used for grafting, which also has the effect of reducing the production cost of high-value-added grafted seedlings having high disease resistance.

[Brief description of the drawings]

FIG. 1 is a schematic explanatory view showing a method for producing a single grafted seedling of tomato of the present invention.

FIG. 2 is a schematic explanatory view showing another specific example of the present invention.

FIG. 3 is a schematic explanatory view showing a method for preparing a grafted seedling with two side branches according to a comparative example.

FIG. 4 is a schematic explanatory view of a single tailoring seedling grafting method by simultaneous production of two strains in a comparative example.

[Explanation of symbols]

 1a, 1b Cotyledon 2a Main leaf first leaf A Hogi seedling 3, 4 Cutting line A-1 Upper tier A-2 Middle tier A-3 Lower cored seedling B-1 Rootstock B-2 Rootstock seedlings 5a, 5b Side branches A-2a Saw with one cotyledon 1a A-2b Saw with one cotyledon 1b B-2 Rootstock seedling B-3 Rootstock seedling 7 Graft support 8 Sprayer 9 Cucumber mosaic virus attenuated virus

[Table 5]

Continued on the front page (72) Inventor Eiji Ichihashi 4-13 Nihonbashi Koamicho, Chuo-ku, Tokyo Inside Delmont Japan (72) Inventor Eiji Takaoka 4-13 Nihonbashi Koamicho, Chuo-ku, Tokyo Japan Del Monte Stock In-company (72) Inventor Haruki Sayama 4-13 Nihonbashi Koamicho, Chuo-ku, Tokyo Inside Del Monte Co., Ltd.

Claims (4)

[Claims] 1. A scion seedling (A) having two cotyledons (1a, 1b) and a first leaf (2a) of a true leaf, between the cotyledon and the first leaf of the true leaf and below the cotyledon, respectively. The upper leaflet (A-1) having the first leaves (2a) of the true leaves, the middle leaflet (A-2) having two cotyledons, and the lower seedling (A-3)
And the upper step scion part (A-1) is grafted to the rootstock seedling (B-1), and the middle step splint part (A-2) is divided into another rootstock seedling (B-2). ), And at the same time as the graft,
Before the grafting or after the grafting, 2 of the middle step xylem (A-2)
One tailored grafted seedling of tomato obtained by removing one of the cotyledons (1a, 1b) from its root and extending the side branch (5a) from the root of the remaining cotyledon. 2. A middle cotyledon (A-2) having two cotyledons (1a, 1b) is divided into two in the axial direction to form one cotyledon (1).
Two scions having a or 1b) (A-2a, A-2b)
And each of these scions is divided into two rootstock seedlings (B-2,
B-3), grafted on the side of cotyledon root (5a, 5b)
The single-seed grafted seedling of tomato according to claim 1, wherein the seedling is expanded. 3. The single grafted tomato seedling of claim 1, wherein the scion seedlings are scion seedlings belonging to the seedling stage of two to three true leaves. 4. The single grafted tomato seedling of tomato according to claim 1, wherein the scion seedling is a scion seedling inoculated with an attenuated virus of cucumber mosaic virus.