JPH09500652A - 固形腫瘍悪性疾患治療用の細胞ワクチンおよび使用法 - Google Patents
固形腫瘍悪性疾患治療用の細胞ワクチンおよび使用法Info
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- JPH09500652A JPH09500652A JP7505901A JP50590195A JPH09500652A JP H09500652 A JPH09500652 A JP H09500652A JP 7505901 A JP7505901 A JP 7505901A JP 50590195 A JP50590195 A JP 50590195A JP H09500652 A JPH09500652 A JP H09500652A
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.(a) 内部固形腫瘍に照射して、腫瘍由来抗原を放出させ; (b) 複数の腫瘍由来抗原を単離し; (c) 白血球調製物を、光活性化可能な物質の存在下で照射して、光不活性 化白血球調製物を生ぜしめ; (d) 前記複数の腫瘍由来抗原を、前記光不活性化白血球調製物と、細胞ワ クチンを生じる条件下で接触させ;そして (e) 前記患者に前記細胞ワクチンを投与することよりなる; 内部固形腫瘍の患者を治療するための放射線療法の効果を向上させる方法。 2.ワクチンを、X線照射療法が可能な内部固形腫瘍の患者に投与する、請求 項1記載の方法。 3.内部固形腫瘍が、肺、胸部、卵巣、子宮、前立腺、睾丸、肝臓、膵臓、胃 、咽頭の鱗状細胞癌腫、線維肉腫、腎臓、膀胱、脳、脊髄、悪性メラノーマおよ び皮膚の転移性鱗状細胞癌腫からなる群から選択される癌に関連するものである 、請求項2記載の方法。 4.ワクチンが、コンピタントな免疫システムを有する患者に投与される、請 求項1記載の方法。 5.ワクチンが、CD8陽性T細胞の絶対レベルがほぼ正常な患者に投与され る、請求項4記載の方法。 6.内部固形腫瘍への照射が、全照射量100ないし約600ラドで腫瘍に照 射して行われる、請求項1記載の方法。 7.複数の腫瘍由来抗原の単離が、全血からの単離である、請求項1記載の方 法。 8.腫瘍由来抗原の濃度が豊富化された抗原調製物を調製する工程をさらに含 む、請求項1記載の方法。 9.照射した白血球調製物と接触させる前に、複数の腫瘍由来抗原を貯蔵する 工程をさらに含む請求項1記載の方法。 10.白血球調製物が、患者から単離される、請求項1記載の方法。 11.白血球調製物が複数のリンパ球を含む、請求項10記載の方法。 12.リンパ球調製物が複数の単球またはB細胞を含む、請求項10記載の方 法。 13.前記調製物を照射に暴露する前に、単球またはB細胞を培養する工程を さらに含む、請求項12記載の方法。 14.白血球調製物を、血液、リンパ液、骨髄、リンパ系器官の組織および組 織培養液からなる群から選択される供給源から単離される、請求項11記載の方 法。 15.白血球調製物の照射への暴露が、ソラレン(psoralen)の存在 下で行われる、請求項1記載の方法。 16.ソラレンが、8−メトキシソラレン、アミノメチルトリメチルソラレン 、5−メトキシソラレンおよびトリメチルソラレンからなる群から選択される、 請求項15記載の方法。 17.ソラレンが、8−メトキシソラレンである、請求項16記載の方法。 18.白血球調製物の照射への暴露が、該調製物をウルトラバイオレットA照 射または可視光線に暴露することよりなる、請求項15記載の方法。 19.白血球調製物を、約420nmより大きい波長の可視光線に曝露する、請 求項18記載の方法。 20.複数の腫瘍由来抗原と光不活性化白血球調製物との接触を、約22℃な いし約30℃の温度で行う、請求項1記載の方法。 21.細胞ワクチンを、患者への投与の前に貯蔵する工程をさらに含む、請求 項1記載の方法。 22.細胞ワクチンの投与が、患者の血流中へ注射することよりなる、請求項 1記載の方法。 23.細胞ワクチンの投与が、皮内注射することよりなる、請求項1記載の方 法。 24.(a) 固形腫瘍由来の複数の抗原を単離し; (b) 白血球調製物を光活性化可能な物質の存在下に照射して、光不活性 化された白血球調製物を生ぜしめ;そして (c) 前記腫瘍由来の複数の抗原を、前記光不活性化白血球調製品と、細 胞ワクチンを生ぜしめる条件下で接触させることよりなる; 内部固形腫瘍の患者に投与するための細胞ワクチンの製造方法。 25.(1) 光不活性化した複数の白血球と混合された固形腫瘍由来の複数の抗 原を含む混合物の有効量;および (2) 薬学的に許容される担体を含有してなる; 内部固形腫瘍の患者を治療する照射療法の効果を向上させる細胞ワクチン。 26.白血球が単球またはB細胞である、請求項25記載のワクチン。 27.(a) 固形腫瘍を照射して腫瘍由来抗原を放出させ; (b) 該腫瘍由来の複数の抗原を単離し; (c) 各々の抗原提示細胞が患者への投与に適する複数の抗原提示細胞を 含む細胞調製物を処理して、該細胞による主要組織適合性複合体分子の発現を向 上させ; (d) 前記複数の腫瘍由来抗原を、前記処理された細胞調製物と、複数の 抗原関連抗原提示細胞を生ぜしめる条件下で接触させ;そして (e) 該複数の抗原関連抗原提示細胞を患者に投与することよりなる、 内部固形腫瘍の患者の治療のための放射線療法の効果を向上させる方法。 28.細胞調製物の処理が、該調製物を約22℃ないし30℃の温度に付する ことよりなる、請求項27記載の方法。 29.細胞調製物の処理が、該細胞調製物を光活性化可能な物質の存在下で照 射することよりなる、請求項27記載の方法。 30.細胞調製物の処理が、主要組織適合性分子の発現を誘発することが知ら れている一種もしくはそれ以上のサイトカインと該細胞調製物とを接触させるこ とからなる、請求項27記載の方法。 31.(a) 複数の腫瘍由来抗原をin vivoで放出させ; (b) 該複数の腫瘍由来抗原を単離し; (c) 白血球調製物を光活性化可能な物質の存在下で照射に暴露して、光 不活性化された白血球調製物を生ぜしめ; (d) 前記複数の腫瘍由来抗原を前記光不活性化白血球調製物と、細胞ワ クチンを生ぜしむる条件下で接触させ;そして (e) 該細胞ワクチンを患者に投与することよりなる; 内部固形腫瘍の患者の腫瘍特異的抗原に対する免疫システムの応答を向上させる 方法。 32.in vivoにおける複数の腫瘍由来抗原の放出が、腫瘍を物理的に 操作すること、血管内化学剤灌流、フォトフリンおよびレーザー光での腫瘍の破 壊からなる群の放出方法から選択される、請求項31記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/100,691 US5820872A (en) | 1992-11-18 | 1993-07-30 | Methods and compositions for improving the effectiveness of X-irradiation therapy for the treatment of an internal solid tumor |
US08/100,691 | 1993-07-30 | ||
PCT/US1994/008301 WO1995003814A1 (en) | 1993-07-30 | 1994-07-25 | Cellular vaccine and methods of use for the treatment of solid tumor malignancies |
Publications (1)
Publication Number | Publication Date |
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JPH09500652A true JPH09500652A (ja) | 1997-01-21 |
Family
ID=22281052
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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JP7505901A Pending JPH09500652A (ja) | 1993-07-30 | 1994-07-25 | 固形腫瘍悪性疾患治療用の細胞ワクチンおよび使用法 |
Country Status (7)
Country | Link |
---|---|
US (1) | US5820872A (ja) |
EP (1) | EP0719150B1 (ja) |
JP (1) | JPH09500652A (ja) |
AT (1) | ATE260666T1 (ja) |
DE (1) | DE69433590T2 (ja) |
ES (1) | ES2213158T3 (ja) |
WO (1) | WO1995003814A1 (ja) |
Cited By (1)
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JP2007130488A (ja) * | 1999-10-14 | 2007-05-31 | Becton Dickinson & Co | ニードルアセンブリ、これを具えた皮内移送装置 |
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US5651993A (en) * | 1992-11-18 | 1997-07-29 | Yale University | Specific immune system modulation |
US5462733A (en) * | 1993-02-04 | 1995-10-31 | Yale University | Immune system modulation using psoralens activated with visible light |
US8021667B2 (en) * | 1994-11-16 | 2011-09-20 | Macfarlane Burnet Institute For Medical Research And Public Health Ltd | Compositions for immunotherapy and uses thereof |
US5882328A (en) * | 1995-01-13 | 1999-03-16 | Qlt Phototherapeutics, Inc. | Method to prevent transplant rejection |
CN1192157A (zh) * | 1995-01-13 | 1998-09-02 | 夸德拉逻辑技术股份有限公司 | 防止移植排斥的方法 |
JP2000508628A (ja) * | 1996-03-22 | 2000-07-11 | エール・ユニバーシティ | 対象体内で免疫応答を誘導する方法 |
CA2250919C (en) * | 1996-03-29 | 2008-05-13 | Therakos, Inc. | Photopheresis treatment of leukocytes |
US6228995B1 (en) | 1996-04-09 | 2001-05-08 | Therakos, Inc. | Method for removal of psoralens from biological fluids |
DE19635086C1 (de) * | 1996-08-30 | 1998-02-26 | Rheinische Braunkohlenw Ag | Verfahren zum Betrieb eines mit Braunkohle befeuerten Kraftwerkes sowie ein derartiges Kraftwerk |
BR9712988A (pt) * | 1996-10-11 | 2000-10-24 | Univ California | Imunoterapia do câncer usando células tumorais combinadas com mistura de linfócitos |
US6465251B1 (en) * | 1996-11-13 | 2002-10-15 | Dana-Farber Cancer Institute, Inc. | Method of promoting b-cell proliferation and activation with CD40 ligand and cyclosporin |
JP4198221B2 (ja) * | 1997-06-13 | 2008-12-17 | 株式会社半導体エネルギー研究所 | 光学活性化合物、反強誘電性液晶組成物、反強誘電性液晶組成物のしきい値を低減する方法及び光学活性化合物の製造方法 |
DK0994743T3 (da) * | 1997-07-10 | 2006-10-02 | Therakos Inc | Behandling af inflammatoriske lidelser i tarm eller urinblære |
DK1027063T3 (da) * | 1997-09-29 | 2009-06-02 | Macfarlane Burnet Inst For Med | Mannosereceptorbærende cellelinje og antigensammensætning |
GB9905911D0 (en) * | 1999-03-15 | 1999-05-05 | Photocure As | Method |
US7109031B2 (en) * | 1999-04-20 | 2006-09-19 | Yale University | Methods for inducing the differentiation of monocytes into functional dendritic cells and immunotherapeutic compositions including such dendritic cells |
CA2368855C (en) | 1999-04-20 | 2012-05-29 | Richard Leslie Edelson | Differentiation of monocytes into functional dendritic cells |
US20050084966A1 (en) * | 1999-04-20 | 2005-04-21 | Edelson Richard L. | Methods for inducing the differentiation of blood monocytes into functional dendritic cells |
US20080241815A1 (en) * | 1999-04-20 | 2008-10-02 | Edelson Richard L | Methods for Inducing the Differentiation of Blood Monocytes into Functional Dendritic Cells |
US20050158856A1 (en) * | 1999-04-20 | 2005-07-21 | Edelson Richard L. | Methods for producing functional antigen presenting dendritic cells using biodegradable microparticles for delivery of antigenic materials |
US6219584B1 (en) * | 1999-07-09 | 2001-04-17 | Therakos, Inc. | Method and system for determining an effective amount of light energy to delivery to fluids having targets for the light energy |
US6495366B1 (en) | 1999-09-03 | 2002-12-17 | Therakos, Inc. | Uninterrupted flow pump apparatus and method |
US20110039943A1 (en) * | 2005-03-14 | 2011-02-17 | Robert Alonso | Methods for treating skin disorders with topical nitrogen mustard compositions |
US8501818B2 (en) * | 2005-03-14 | 2013-08-06 | Ceptaris Therapeutics, Inc. | Stabilized compositions of alkylating agents and methods of using same |
US7872050B2 (en) * | 2005-03-14 | 2011-01-18 | Yaupon Therapeutics Inc. | Stabilized compositions of volatile alkylating agents and methods of using thereof |
EP3494960B1 (en) | 2008-03-27 | 2020-11-25 | Helsinn Healthcare SA | Stabilized compositions of alkylating agents and methods of using same |
US20120052544A1 (en) * | 2010-08-24 | 2012-03-01 | Mcgarry Thomas J | Enucleation of cells with psoralens |
AU2017213801B2 (en) * | 2016-02-02 | 2022-04-14 | Duke University | Phosphor-containing drug activator, suspension thereof, system containing the suspension, and methods for use |
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US4670386A (en) * | 1980-05-23 | 1987-06-02 | Stephen Sugaar | Cancer tests using tumor antigen generated lymphokines and compositions |
US4753884A (en) * | 1986-01-28 | 1988-06-28 | Novagene, Inc. | Pseudorabies virus mutants, vaccines containing same, methods for the production of same and methods for the use of same |
US4838852A (en) * | 1987-03-27 | 1989-06-13 | Therakos, Inc. | Active specific immune suppression |
US4895717A (en) * | 1987-07-10 | 1990-01-23 | The United States Of America As Represented By The Secretary Of Agriculture | Revertant serotype 1 Marek's disease vaccine |
US5147289A (en) * | 1990-03-29 | 1992-09-15 | Therakos, Inc | Non-specific immune system enhancement |
US5651993A (en) * | 1992-11-18 | 1997-07-29 | Yale University | Specific immune system modulation |
US5462733A (en) * | 1993-02-04 | 1995-10-31 | Yale University | Immune system modulation using psoralens activated with visible light |
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- 1993-07-30 US US08/100,691 patent/US5820872A/en not_active Expired - Lifetime
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- 1994-07-25 JP JP7505901A patent/JPH09500652A/ja active Pending
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- 1994-07-25 WO PCT/US1994/008301 patent/WO1995003814A1/en active IP Right Grant
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- 1994-07-25 AT AT95906825T patent/ATE260666T1/de active
- 1994-07-25 DE DE69433590T patent/DE69433590T2/de not_active Expired - Lifetime
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007130488A (ja) * | 1999-10-14 | 2007-05-31 | Becton Dickinson & Co | ニードルアセンブリ、これを具えた皮内移送装置 |
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EP0719150B1 (en) | 2004-03-03 |
EP0719150A4 (en) | 1998-07-29 |
EP0719150A1 (en) | 1996-07-03 |
DE69433590T2 (de) | 2004-08-05 |
DE69433590D1 (de) | 2004-04-08 |
ATE260666T1 (de) | 2004-03-15 |
WO1995003814A1 (en) | 1995-02-09 |
US5820872A (en) | 1998-10-13 |
ES2213158T3 (es) | 2004-08-16 |
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