JPH09216827A - Hair-mother cell activator and hair growth stimulant composition formulated therewith - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a hair-mother cell activator useful for preventing depilation and for growing the hair, containing, as active ingredient, a water extract from a specific kind of plant, and to obtain a hair growth stimulant composition formulated with the activator. SOLUTION: This hair-mother cell activator contains, as active ingredient, a water extract from scutellariae radix, a kind of plant belonging to Labiatae (e.g. a water crude extract from the plant, a purified fraction of the extract). The purified fraction is pref. a fraction <=13000 in a molecular weight cut off obtained by ultrafiltration of the above-mentioned crude extract, or a fraction 1000-3000 in molecular weight obtained by get filtration of the above fraction. The other objective hair growth stimulant composition contains the active ingredient at 0.001-10wt.% or so in terms of the water crude extract on a solid basis (0.001-10wt.% for the fraction <=13000 in molecular weight, 0.0001-1wt.% for the fraction 1000-3000 in molecular weight). This hair growth stimulant composition can be made into a formulation such as hair cream, hair lotion or hair tonic.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a hair mother cell activator useful for preventing hair loss and hair growth, and a hair nourishing composition containing the same.

[0002]

2. Description of the Related Art Hair nourishing cosmetics containing various chemicals have been known to remove or reduce the causes of baldness and hair loss. In these hair nourishing cosmetics, for example, in order to improve the blood flow to the hair root, assembly extracts and acetylcholine derivatives are blended, and female hormone agents such as estradiol as an anti-male hormone are blended. , Hinokitio-to improve scalp metabolism
, Etc. have been blended, and attempts have been made to prevent and treat alopecia.

However, the mechanism of hair loss and hair growth is very complicated, and it is sufficient to prevent hair loss by promoting blood circulation, deactivating male hormones or improving scalp metabolism as in conventional hair nourishing compositions. Hair growth effect is not obtained.
On the other hand, in androgenetic alopecia and senile alopecia, the hair follicles are small and the activity of hair mother cells that regenerate hair is reduced. Therefore, it is considered that activating hair mother cells is effective in preventing hair loss and hair growth.

[0004]

The object of the present invention is to prevent hair loss by activating hair mother cells with reduced activity,
It is to provide a hair mother cell activating agent useful for hair growth and a hair nourishing agent composition containing the same.

[0005]

In view of such circumstances, the inventors of the present invention have conducted diligent research on a component having an activity of activating hair mother cells, and as a result, have found that a water extract of sardine, especially The present inventors have found that the molecular weight fraction of (1) has a very excellent hair mother cell activating effect, and completed the present invention.

[0006] That is, the present invention provides a hair matrix activating agent containing a water extract of Astragalus vulgaris and a hair nourishing composition containing the same. According to the present invention, it is possible to provide a hair matrix activator useful for preventing hair loss and hair growth, and a hair nourishing agent composition containing the same, by solving the above problems.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The water extract of Scutellariae sinensis used as an active ingredient of the hair mother cell activator of the present invention is not particularly limited, and examples thereof include a crude extract of Scutellariae sinensis and a purified fraction thereof. As a purified fraction, a fraction having a molecular weight cut-off of 13,000 or less obtained by ultrafiltration of a crude water extract, and a molecular weight of 1,000 to 30, which is obtained by subjecting this to further gel filtration
The 00 fraction is preferred.

The water extract of Scutellaria baicalensis of the present invention is, for example,
Scutellaria baicalensis Georgi (Lamiaceae)
[Root part of] can be obtained by simply performing rough extraction with water. For example, 10 parts by weight of water is added to 1 part of dried product (finely chopped or crushed) of sardine to obtain room temperature to 80%.
Examples include those extracted in the temperature range of ° C for about 1 to 6 hours while gently stirring. The crude extract can be used in the state of the crude extract as it is, or in the state of a liquid or solid which is concentrated and dried to dryness according to a conventional method.

[0009] A preferred water extract of Scutellaria baicalensis is a fraction having a molecular weight of 13,000 or less obtained by subjecting the crude water extract of Scutellariae sinensis described above to ultrafiltration.

Further, the above-mentioned ultrafiltrate is further subjected to gel filtration using a column such as Sephadex G-25 or the like to obtain a molecular weight of 1,000 to 30,
The 00 fraction is also preferred. These fractions can be used as they are or in the state of concentrated or dried liquid or solid according to a conventional method.

The hair mother cell activating agent of the present invention may be the water extract itself of Scutellariae sinensis obtained as described above.
Using it as an active ingredient, for example, a known excipient, a solvent, an antiseptic, a chelating agent, an additive such as a perfume, and the like are appropriately mixed,
It may be a liquid or solid dosage form obtained by a conventional method. The hair mother cell activator of the present invention is not particularly limited, but usually, at least as a solid content concentration of a crude water extract, 0.001% by weight (solid content of a fraction having a molecular weight of 13,000 or less). Concentration is 0.001% by weight, molecular weight is 1.0
The solid concentration of the fraction of 0 to 3,000 is 0.0001.
% By weight).

The hair nourishing agent composition of the present invention is characterized in that the hair mother cell activator of the present invention is added as an active ingredient, and other ingredients are usually added to known hair nourishing agent compositions. Either component may be used. The amount of the active ingredient contained in the hair nourishing agent composition is not particularly limited, but is usually 0.0001 to 10 as the solid content concentration of the crude water extract.
% By weight (as a solid content concentration of a fraction having a molecular weight of 13,000 or less, 0.001 to 10% by weight, a molecular weight of 1,000 to 3,
The solid content concentration of the 000 fraction is 0.0001 to 1% by weight). The hair nourishing agent composition according to the present invention can be made into any conventionally known dosage form such as a hair cream, a hair lotion, and a hairnic according to a conventional method.

[0013]

EXAMPLES Next, the present invention will be described in more detail with reference to examples and test examples, but the present invention is not limited thereto. Example 1 Production of a water extract of Scutellaria baicalensis 10 kg of purified water was added to 1 Kg of Scutellariae powder to give 5
Extract at 0 ° C. for 6 hours with gentle stirring. By filtering the extract, a water extract of Scutellaria baicalensis is obtained.

Example 2 Purification by ultrafiltration An ultrafiltrate is obtained by passing the above extract through an ultrafiltration membrane (molecular cutoff of 13,000).

Example 3 Purification by gel filtration The above-mentioned ultrafiltrate was passed through a Sephadex G-25 column, and a fraction having a molecular weight of 1,000 to 3,000 was collected to obtain a gel filtrate. obtain.

Reference Example 1 Ethanol Extract Addition of 10 kg of ethanol to 1 kg of powdered sardine,
Extract at 50 ° C. for 6 hours with gentle stirring. By filtering the extract, the ethanol extract of Scutellaria baicalensis is obtained.

Test Example 1 Test for Action on Proliferation of Cultured Hair Mother Cells Hair tissues of mice were collected and cultured in serum-free medium (Tanigaki N. et al. (1990) Arch. Dermatol. Res.
282: 402-407). The water extract of Astragalus gondii obtained in Example 1, the ethanol extract obtained in Reference Example 1 or the fractions of the water extracts obtained in Examples 2 and 3 were dissolved in a medium to give 0.05 to 50 μg. The concentration was adjusted to be / ml. On the first day of culturing, a water extract, an ethanol extract, or a fraction of the water extract was added. After culturing for 6 days, the cells were detached from the dish and the number of cells was determined. The effect of the water extract of Scutellariae sinensis or its fraction was 100% when the number of cells in the control group (group cultured only in serum-free medium) was 100%.
It was expressed as a relative ratio when it was defined as%. The results are shown in Tables 1 and 2.

Table 1 Cell number Proliferation Specimen Relative value (%) Promotion rate (%) Media only (control group) 100-water extract 0.5 μg / ml 125 25 5 146 46 50 152 52 52 ethanol extract 0.5 μg / Ml 107 7 5 108 8 50 101 1

As is clear from Table 1, the water extract of Astragalus membranaceus had a remarkable effect of promoting the growth of cultured hair mother cells.

Table 2 Cell number Proliferation Specimen Relative value (%) Acceleration rate (%) Media only (control group) 100-water extract Fraction with molecular weight of 13,000 or less 0.5 μg / ml 144 445 165 655 50 172 72 Fraction with a molecular weight of 13,000 or more 0.5 μg / ml 105 5 5 110 10 50 112 12 12 Fraction with a molecular weight of 1,000 to 3,000 0.5 μg / ml 149 49 5 180 80 50 177 77 77

As is clear from Table 2, among the water extract of Astragalus membranaceus, the fraction having a molecular weight of 13,000 or less, particularly the fraction having a molecular weight of 1,000 to 3,000, has an excellent effect of promoting the growth of cultured hair mother cells. Had.

Test Example 2 Hair Growth Acceleration Test Using C3H Mice Agonaceous water extract, ethanol extract or a fractionated solution of water extract was dissolved in water and adjusted to a concentration of 0.1% by weight. Approximately 2 × on the back of 8-week-old C3H male mice
After cutting the hair to a size of 4 cm, from the next day, apply the solution for 1 day and 1 day.
Each time, for 15 days, 0.1 ml of each was applied to the shaved part, and the effect on the hair regrowth at that part was examined. The effect of the water extract of Scutellariae sinensis or its fraction was expressed as a relative ratio when the hair weight of the control group (water application group) was 100%. The results are shown in Tables 3 and 4.

Table 3 Hair weight Hair growth test body relative value (%) Acceleration rate (%) Water (control group) 100-Water extract 236 136 Ethanol extract 180 80

As is clear from Table 3, the Scutellariae Radix extract has a remarkable hair growth promoting effect as compared with the control.

Table 4 Hair weight Hair growth test body Relative value (%) Acceleration rate (%) Water (control group) 100-Water extract Fraction with molecular weight of 13,000 or less 372 272 Fraction with molecular weight of 13,000 or more 141 41 Fractions with a molecular weight of 1,000 to 3,000 398 298

As is clear from Table 4, the fractions having a molecular weight of 13,000 or less in the water extract of Scutellariae japonica, especially the molecular weight of 1,
The 000 to 3,000 fraction has a superior hair growth promoting effect as compared to the control.

Example 4 A hair tonic was produced by a conventional method according to the following formulation.

Example 5 A lotion was prepared by a conventional method according to the following formulation.

Example 6 A hair cream was produced by a conventional method according to the following formulation.

[0030]

EFFECTS OF THE INVENTION The hair mother cell activating agent of the present invention has excellent hair-retaining effects such as hair loss prevention and hair growth effect, and is applied to a hair-retaining agent composition used in the fields of pharmaceuticals, quasi drugs and cosmetics. can do.

Claims (8)

[Claims] 1. A hair mother cell activator containing a water extract of Astragalus membranaceus. 2. A hair mother cell activator, wherein the extract is a crude water extract of Scutellaria baicalensis. 3. The hair mother cell activator according to claim 1, wherein the extract is a fraction having a molecular weight of 13,000 or less, which is obtained by subjecting a water crude extract of Astragalus membranaceus to ultrafiltration. 4. An extract, which is obtained by subjecting the fraction according to claim 3 to gel filtration, has a molecular weight of 1,000 to 3,000.
The hair matrix activating agent according to claim 1, which is a fraction 0. 5. A hair nourishing agent composition containing the hair mother cell activator according to claim 1. 6. A hair nourishing agent composition containing the hair mother cell activator according to claim 2. 7. A hair nourishing agent composition containing the hair mother cell activator according to claim 3. 8. A hair nourishing agent composition containing the hair mother cell activator according to claim 4.