JPH09216828A - Hair-mother cell activator and hair growth stimulant formulated therewith - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a hair-mother cell activator useful for preventing depilation and for growing the hair, containing, as active ingredient, a specific extract from scutellaria root, and to obtain a hair growth stimulant formulated with the activator. SOLUTION: This hair-mother cell activator contains as active ingredient, an extract obtained by subjecting scutellaria root, a kind of plant belonging to Labiatae, to extraction with 2-5C glycol(s) (e.g. 1,3-butylene glycol and/or 1,2-propylene glycol) or its mixture with water. The extract is pref. a fraction obtained by ultrafiltration of the above extract and having a molecular weight cutoff of <=13000. An alternative preferable extract is a fraction 1000-3000 in molecular weight obtained by get filtration, using a column such as Sephadex G-25, of the extract obtained by the above ultrafiltration. It is preferable that the concentration of the active ingredient is 0.001-10wt.% or so in the glycol extract on a solid basis. The other objective hair growth stimulant can be made into a formulation such as hair cream, hair lotion or hair tonic.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a hair mother cell activator useful for preventing hair loss and hair growth, and a hair nourishing agent containing the same.

[0002]

2. Description of the Related Art Hair nourishing cosmetics containing various chemicals have been known to remove or reduce the causes of baldness and hair loss. In these hair nourishing cosmetics, for example, in order to improve the blood flow to the hair root, assembly extracts and acetylcholine derivatives are blended, and female hormone agents such as estradiol as an anti-male hormone are blended. , Hinokitio-to improve scalp metabolism
, Etc. have been blended, and attempts have been made to prevent and treat alopecia.

However, the mechanism of hair loss and hair growth is very complicated. Sufficient hair loss prevention and hair growth can be achieved only by promoting blood circulation, deactivating male hormones or improving scalp metabolism like conventional hair nourishing agents. No effect has been obtained. On the other hand, in androgenetic alopecia and senile alopecia, the hair follicles are small and the activity of hair mother cells that regenerate hair is reduced. Therefore, it is considered that activating hair mother cells is effective in preventing hair loss and hair growth.

[0004]

The object of the present invention is to prevent hair loss by activating hair mother cells with reduced activity,
To provide a hair mother cell activating agent useful for hair growth and a hair nourishing agent containing the same.

[0005]

In view of such circumstances, the inventors of the present invention have conducted extensive studies as to a component having an activity of activating hair mother cells, and as a result, a glycol extract of gonosa, The present inventors have found that a specific molecular weight fraction has a very excellent hair mother cell activating effect, and completed the present invention.

[0006] That is, the present invention provides a hair mother cell activating agent containing a glycolic acid extract of Auregon and a hair nourishing agent containing the same. According to the present invention, it is possible to solve the above problems and provide a hair mother cell activator useful for preventing hair loss and hair growth, and a hair nourishing agent containing the same.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The glycol extract of Scutellariae sinensis used as an active ingredient of the hair mother cell activating agent of the present invention is not particularly limited, and for example, crude extract of Scutellariae sinensis and a purified fraction thereof Examples of the purified fraction include a fraction having a molecular weight cut off of 13,000 or less obtained by ultrafiltration of a crude extract of glycols, and a fraction having a molecular weight of 1,000 to 3,000 obtained by further gel filtration thereof. preferable.

The glycol extract of Scutellariae of the present invention may be obtained by simply and crudely extracting Scutellaria baicalensis Georgi (root of Lamiaceae) with an extraction solvent. For example, the solvent used for extraction is a glycol having 2 to 5 carbon atoms, preferably one selected from 1,3-butylene glycol and 1,2-propylene glycol, or a mixed solvent thereof. The glycols may be mixed with water. For example, 10 parts by weight of the extraction solvent is added to 1 part of dried product (finely chopped or crushed) of sardine and extracted with gentle stirring at a temperature range of room temperature to 80 ° C. for about 1 to 6 hours. To be The crude extract can be used in the state of the crude extract as it is, or in the state of a liquid or solid which is concentrated and dried in accordance with a conventional method.

[0009] The preferred glycolic extract of Scutellariae sinensis is the fraction having a molecular weight of 13,000 or less obtained by subjecting the crude extract of Scutellariae sinensis above to ultrafiltration.

Further, the above-mentioned ultrafiltrate is further subjected to gel filtration using a column such as Sephadex G-25 or the like to obtain a molecular weight of 1,000 to 30,
The 00 fraction is also preferred. These fractions can be used as they are or in the state of concentrated or dried liquid or solid according to a conventional method.

The hair mother cell activator of the present invention may be the glycol extract of Scutellariae sinensis itself obtained as described above, or it may be used as an active ingredient, for example, known excipients, solvents, preservatives. A liquid or solid dosage form obtained by a conventional method may be prepared by appropriately adding additives such as agents, chelating agents, and fragrances. The hair mother cell activator of the present invention is not particularly limited, but usually, at least as a solid content concentration of the glycol crude extract, 0.001% by weight (solid content of a fraction having a molecular weight of 13,000 or less). As a minute concentration, 0.00
1% by weight, and the solid content of the fraction having a molecular weight of 1,000 to 3,000 is preferably 0.0001% by weight).

The hair nourishing agent of the present invention comprises the hair mother cell activating agent of the present invention as an active ingredient,
The other compounding ingredients may be any of the ingredients usually incorporated in known hair nourishing agents. The amount of active ingredient contained in the hair nourishing agent is
Although not particularly limited, it is usually 0.0001 to 10% by weight as the solid content concentration of the glycols crude extract (as the solid content concentration of the fraction having a molecular weight of 13,000 or less, 0.0
The solid content of the fraction having a molecular weight of 01 to 10% by weight and a molecular weight of 1,000 to 3,000 is 0.0001 to 1% by weight). The hair nourishing agent according to the present invention can be made into any conventionally known dosage form such as a hair cream, a hair lotion and a hair nick according to a conventional method.

[0013]

EXAMPLES Next, the present invention will be described in more detail with reference to examples and test examples, but the present invention is not limited thereto. Example 1 Manufacture of a glycolic extract of Scutellariae sinensis 10 kg of 1,3-butylene glycol was added to 1 kg of powdered Scutellariae and extracted with gentle stirring at 50 ° C. for 6 hours. By filtering the extract, a 1,3-butylene glycol extract of Auregon is obtained.

Example 2 Purification by ultrafiltration An ultrafiltrate is obtained by passing the above extract through an ultrafiltration membrane (molecular cutoff of 13,000).

Example 3 Purification by gel filtration The above-mentioned ultrafiltrate was passed through a Sephadex G-25 column, and a fraction having a molecular weight of 1,000 to 3,000 was collected to obtain a gel filtrate. obtain.

Reference Example 1 Ethanol Extract Addition of 10 kg of ethanol to 1 kg of powdered sardine,
Extract at 50 ° C. for 6 hours with gentle stirring. By filtering the extract, the ethanol extract of Scutellaria baicalensis is obtained.

Test Example 1 Effect test on proliferation of cultured hair matrix cells Hair tissues of mice were collected and cultured in serum-free medium (Tanigaki N. et al. (1990) Arch. Dermatol. Res.
282: 402-407). The Scutellariae Radix extract or its fraction was dissolved in the medium and adjusted to a concentration of 0.05 to 50 μg / ml. On the first day of the culture, the extract or its fraction was added. After culturing for 6 days, the cells are shaken.
The cells were peeled off from the cells and the number of cells was determined. The effect of the glycolic acid extract of Scutellaria sinensis or its fraction was expressed as a relative ratio when the number of cells in the control group (group cultured in serum-free medium alone) was 100%. The results are shown in Table 1.

Table 1 Cell number Proliferation Specimen Relative value (%) Promotion rate (%) Medium only (control group) 100-1,3-butylene glycol extract 0.5 μg / ml 121 2 15 139 39 39 50 140 40 1 , 2-Propylene glycol extract 0.5 μg / ml 115 15 5 148 48 50 143 43 Ethanol extract 0.5 μg / ml 107 7 5 108 8 50 101 1 1,3-butylene glycol extract Molecular weight 13,000 or less Fraction 0.5 μg / ml 125 25 5 163 63 50 168 68 Fraction with a molecular weight of 13,000 or more 0.5 μg / ml 101 1 5 115 15 50 117 17 17 Fraction with a molecular weight of 1,000-3,000 0.5 μg / Ml 134 34 5 173 73 50 50 156 56

As is apparent from Table 1,
The 3-butylene glycol or 1,2-propylene glycol extract had a growth promoting effect on cultured hair mother cells. In addition, a fraction of the molecular weight of the 1,3-butylene glycol extract of Ougon of 13,000 or less and a molecular weight of 1,
The fraction of 000 to 3,000 had an excellent effect of promoting the growth of cultured hair mother cells.

Test Example 2 Hair Growth Acceleration Test Using C3H Mice Agonaceae extract or a fraction thereof was dissolved in water to give a result of 0.1.
The concentration was adjusted to be 1% by weight. 8 weeks old C3H
The back of male mice was shaved to a size of about 2 × 4 cm, and from the next day, the above solution was applied once a day for 15 days to a shaved part of 0.1.
Each ml was applied, and the effect on hair regeneration at that site was examined. The effect of Scutellaria extract or its fraction is
The relative weight when the hair weight of the control group (1,3-butylene glycol application group) was 100% was expressed. Table 2 shows the results.

Table 2 Hair weight Hair growth Test body relative value (%) Acceleration rate (%) 1,3-butylene glycol (control group) 100-1,3-butylene glycol extract 243 143 1,2-propylene glycol extraction Liquid 230 130 Ethanol extract 180 80 1,3-butylene glycol extract Fraction with a molecular weight of 13,000 or less 355 255 Fraction with a molecular weight of 13,000 or more 129 29 Fraction with a molecular weight of 1,000 to 3,000 384 284

As can be seen from Table 2,
The 3-butylene glycol or 1,2-propylene glycol extract has a hair growth promoting effect as compared with the control. Furthermore, the fractions having a molecular weight of 13,000 or less and the fractions having a molecular weight of 1,000 to 3,000 in the extract of 1,3-butylene glycols of Scutellariae serrata have an excellent hair growth promoting effect as compared with the control. .

Example 4 A hair tonic was produced by a conventional method according to the following formulation. Component% W / V Ougon 1,3-butylene 0.1 Glycol extract (molecular weight 13,000 or less) 95% Ethanol 70 Solubilizer 1 (Polyoxyethylene (60EO) hydrogenated castor oil) Propylene Glycol Le 5 Fragrance Adjusted to 100% trace water

Example 5 A lotion was prepared by a conventional method according to the following formulation. Component% W / V Hydroxyethyl cellulose 0.4 Ethanol 5 1,3-butylene glycol 5 Paraoxybenzoic acid ester 0.2 Ougon / 1,3-butylene 0.1 Glycol extract (molecular weight 13,000 or less) Fragrance 1 Adjust to 100% water

Example 6 A hair cream was produced by a conventional method according to the following formulation. Component% W / V Beeswax 5 Lanolin 4 Vaseline 5 Liquid paraffin 33 Emulsifier 4 (Polyoxyethylene (20EO) sorbitan monostearate) Paraoxybenzoic acid ester 0.2 Perfume Trace augon 1,3-butylene 0.1 Glycol extract (molecular weight 13,000 or less) Borax 1 Water adjusted to 100

Example 7 A hair tonic was produced by a conventional method according to the following formulation. Component% W / V Ougon 50% 1,3-0.1 Butylene glycol extract (Molecular weight 13,000 or less) 95% Ethanol 70 Solubilizer 1 (Polyoxyethylene hydrogenated castor oil [60EO]) Propylene glycol 5 Adjust to 100 perfume

Example 8 A lotion was produced by a conventional method according to the following formulation. Component% W / V Hydroxyethyl cellulose 0.4 Ethanol 5 Butane-1,3-diol 5 Paraoxybenzoic acid ester 0.2 50% of Ougon 1,3-0.1 Butylene glycol extract (molecular weight 13,000 or less) Fragrance 1 Adjust to 100 water

[0028]

EFFECTS OF THE INVENTION The hair matrix activating agent of the present invention has excellent hair-retaining effects such as hair loss prevention and hair growth effects, and can be applied to hair-retaining agents used in the fields of pharmaceuticals, quasi drugs and cosmetics. You can

Claims (10)

[Claims] 1. A hair mother cell activator containing a glycolic extract of sardine. 2. A hair mother cell activator in which the extract is a crude extract of glycols of sardine. 3. The extract has a molecular weight of 13,000, which is obtained by subjecting a crude extract of glycols of Scutellaria sinensis to ultrafiltration.
The hair matrix activating agent according to claim 1, which is the following fraction. 4. An extract, which is obtained by subjecting the fraction according to claim 3 to gel filtration, has a molecular weight of 1,000 to 3,000.
The hair matrix activating agent according to claim 1, which is a fraction 0. 5. The hair mother cell activator according to any one of claims 1 to 4, wherein the glycol is 1,3-butylene glycol or 1,2-propylene glycol. 6. A hair nourishing agent containing the hair mother cell activator according to claim 1. 7. A hair nourishing agent containing the hair mother cell activator according to claim 2. 8. A hair nourishing agent containing the hair mother cell activator according to claim 3. 9. A hair nourishing agent containing the hair mother cell activator according to claim 4. 10. A hair nourishing agent containing the hair mother cell activator according to claim 5.