JPH09173078A - 分子シャペロンを用いる蛋白質の製造方法 - Google Patents

分子シャペロンを用いる蛋白質の製造方法

Info

Publication number
JPH09173078A
JPH09173078A JP8228252A JP22825296A JPH09173078A JP H09173078 A JPH09173078 A JP H09173078A JP 8228252 A JP8228252 A JP 8228252A JP 22825296 A JP22825296 A JP 22825296A JP H09173078 A JPH09173078 A JP H09173078A
Authority
JP
Japan
Prior art keywords
protein
gene encoding
molecular chaperone
cell
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
JP8228252A
Other languages
English (en)
Japanese (ja)
Inventor
Tadayuki Imanaka
忠行 今中
Masahiro Takagi
昌宏 高木
Katsunori Koda
勝典 幸田
Tomoko Kubomi
朋子 久保見
Yan Tsuen
ヤン ツェン
Shinsuke Fujiwara
伸介 藤原
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to JP8228252A priority Critical patent/JPH09173078A/ja
Priority to EP96306713A priority patent/EP0774512A2/en
Publication of JPH09173078A publication Critical patent/JPH09173078A/ja
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
JP8228252A 1995-09-14 1996-08-29 分子シャペロンを用いる蛋白質の製造方法 Withdrawn JPH09173078A (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP8228252A JPH09173078A (ja) 1995-09-14 1996-08-29 分子シャペロンを用いる蛋白質の製造方法
EP96306713A EP0774512A2 (en) 1995-09-14 1996-09-16 A method for production of protein using molecular chaperon

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP23717695 1995-09-14
JP7-237176 1995-09-14
JP8228252A JPH09173078A (ja) 1995-09-14 1996-08-29 分子シャペロンを用いる蛋白質の製造方法

Publications (1)

Publication Number Publication Date
JPH09173078A true JPH09173078A (ja) 1997-07-08

Family

ID=26528143

Family Applications (1)

Application Number Title Priority Date Filing Date
JP8228252A Withdrawn JPH09173078A (ja) 1995-09-14 1996-08-29 分子シャペロンを用いる蛋白質の製造方法

Country Status (2)

Country Link
EP (1) EP0774512A2 (https=)
JP (1) JPH09173078A (https=)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6162626A (en) * 1998-07-31 2000-12-19 Oriental Yeast Co., Ltd. Thermostable glutamate dehydrogenase and process for producing the same
JP2002335959A (ja) * 2001-04-30 2002-11-26 F Hoffmann La Roche Ag フォールディング補助タンパク質の共発現を伴うｉｎｖｉｔｒｏ翻訳系でのタンパク質の発現方法
US6579699B1 (en) 1998-08-11 2003-06-17 Ajinomoto Co., Inc. Method for producing fermentative product and stress-resistant microorganism
JP2005537796A (ja) * 2002-09-04 2005-12-15 イッサム リサーチ ディベロップメント カンパニー オブ ザ ヘブリュー ユニバーシティー オブ エルサレム 変性剤安定および／またはプロテアーゼ耐性シャペロン様オリゴマータンパク質、これらをコードするポリヌクレオチド、これらの使用、およびこれらの比活性を増加させる方法
WO2012027930A1 (zh) * 2010-08-31 2012-03-08 上海交通大学 用于外源蛋白可溶性表达的质粒及其制备和应用方法

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1111602C (zh) * 1998-05-15 2003-06-18 中国科学院上海生物化学研究所 一种使用分子伴侣促进蛋白质分泌的方法
EP1048732A1 (de) * 1999-04-26 2000-11-02 F. Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen
FI109361B (fi) 1999-05-04 2002-07-15 Korpela Timo Mikrobinen proteiinin ilmentämisjärjestelmä
GB9913437D0 (en) * 1999-06-09 1999-08-11 Medical Res Council Fusion proteins
EP1077263A1 (de) 1999-07-29 2001-02-21 F.Hoffmann-La Roche Ag Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen
CN1311236A (zh) * 2000-03-02 2001-09-05 上海博德基因开发有限公司 一种新的多肽——人分子伴侣cpn60蛋白26和编码这种多肽的多核苷酸
CN1324816A (zh) * 2000-05-24 2001-12-05 上海博德基因开发有限公司 一种新的多肽——分子伴侣cpn60蛋白15和编码这种多肽的多核苷酸
CN1324814A (zh) * 2000-05-24 2001-12-05 上海博德基因开发有限公司 一种新的多肽——分子伴侣cpn60蛋白10和编码这种多肽的多核苷酸
DE10046960A1 (de) * 2000-09-22 2002-04-11 Roche Diagnostics Gmbh Verfahren zur Herstellung einer aktiven, heterodimeren AMW-RT in prokaryotischen Zellen
ES2365602T3 (es) 2001-03-05 2011-10-07 Yissum Research Development Company Of The Hebrew University Of Jerusalem Proteínas oligoméricas tipo chaperona, estables a desnaturalizantes y/o resistentes a proteasa, polinucleótidos que codifican las mismas y sus usos.
WO2007007325A2 (en) 2005-07-07 2007-01-18 Fulcrum Sp Ltd. Sp1 polypeptides, modified sp1 polypeptides and uses thereof
US20120064560A1 (en) * 2008-06-16 2012-03-15 Smith Harold E Folding of recombinant proteins via co-expression of archaeal chaperones
IL243839B (en) 2016-01-28 2018-01-31 Sp Nano Ltd Conductive wires
IL243838A (en) 2016-01-28 2017-07-31 Sp Nano Ltd The composition containing sp1 protein and carbon nanoparticles and its uses
KR102185312B1 (ko) * 2017-10-25 2020-12-02 재단법인 지능형 바이오 시스템 설계 및 합성 연구단 목적 단백질의 가용화용 조성물 및 이의 용도
CN116640710B (zh) * 2022-10-08 2024-08-02 科赫生物科技（北京）有限公司 生产鲎凝血因子FGβ＇的菌株、制备方法和应用
CN121405791A (zh) * 2025-12-26 2026-01-27 中国极地研究中心(中国极地研究所) 一种重组胶原蛋白三螺旋折叠与稳定化方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5428131A (en) * 1988-10-24 1995-06-27 Yale University Archaebacterial chaperonin-mediated protein stabilization
NL9102009A (nl) * 1991-11-29 1993-06-16 Stichting Katholieke Univ Produktie van bioactieve peptiden met recombinante cellen.
WO1993025681A1 (en) * 1992-06-11 1993-12-23 New York University A cytoplasmic chaperonin and methods of making and using it
DE4239969A1 (de) * 1992-11-27 1994-06-01 Boehringer Mannheim Gmbh Verfahren zur Stabilisierung von Proteinen

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6162626A (en) * 1998-07-31 2000-12-19 Oriental Yeast Co., Ltd. Thermostable glutamate dehydrogenase and process for producing the same
US6579699B1 (en) 1998-08-11 2003-06-17 Ajinomoto Co., Inc. Method for producing fermentative product and stress-resistant microorganism
JP2002335959A (ja) * 2001-04-30 2002-11-26 F Hoffmann La Roche Ag フォールディング補助タンパク質の共発現を伴うｉｎｖｉｔｒｏ翻訳系でのタンパク質の発現方法
JP2005537796A (ja) * 2002-09-04 2005-12-15 イッサム リサーチ ディベロップメント カンパニー オブ ザ ヘブリュー ユニバーシティー オブ エルサレム 変性剤安定および／またはプロテアーゼ耐性シャペロン様オリゴマータンパク質、これらをコードするポリヌクレオチド、これらの使用、およびこれらの比活性を増加させる方法
WO2012027930A1 (zh) * 2010-08-31 2012-03-08 上海交通大学 用于外源蛋白可溶性表达的质粒及其制备和应用方法

Also Published As

Publication number Publication date
EP0774512A3 (https=) 1997-06-18
EP0774512A2 (en) 1997-05-21

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Effective date: 20031104