JPH08500484A - クローニング及び/または配列ベクター - Google Patents
クローニング及び/または配列ベクターInfo
- Publication number
- JPH08500484A JPH08500484A JP6504840A JP50484094A JPH08500484A JP H08500484 A JPH08500484 A JP H08500484A JP 6504840 A JP6504840 A JP 6504840A JP 50484094 A JP50484094 A JP 50484094A JP H08500484 A JPH08500484 A JP H08500484A
- Authority
- JP
- Japan
- Prior art keywords
- nucleotide sequence
- vector
- cloning
- protein
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.自律増殖ベクター(autonomously replicating vector)(2)に組込まれた 形で少なくとも1つのプロモータヌクレオチド配列(3)と毒素として作用する 融合たんぱくを暗号化する少なくとも1つのヌクレオチド配列(4)とを含み、 いくつかのクローニング部位を含む暗号ヌクレオチド配列(5)とたんぱく毒を 暗号化するヌクレオチド配列(6)とを融合することによって前記ヌクレオチド 配列(4)を得ることを特徴とするクローニング及び/または配列ベクター。 2.自律増殖ベクター(2)が組換えウィルスであることを特徴とする請求の範 囲第1項に記載のベクター。 3.自律増殖ベクター(2)が組換えプラスミドであることを特徴とする請求の 範囲第1項に記載のベクター。 4.自律増殖ベクター(2)が組換えpUCプラスミドであることを特徴とする 請求の範囲第3項に記載のベクター。 5.プロモータヌクレオチド配列(3)がLacオペロンプロモータから成るこ とを特徴とする請求の範囲第1項から第4項までのいずれかに記載のベクター。 6.たんぱく毒を暗号化するヌクレオチド配列(6)と融合するヌクレオチド配 列(5)の特定クローニング部位(unique cloning sites)がクローニングベクタ ーのヌクレオチド 配列の残り部分に存在しないことを特徴とする請求の範囲第1項から第5項まで のいずれかに記載のベクター。 7.たんぱく毒を暗号化するヌクレオチド配列(6)がたんぱくCcdBを暗号 化する野性型遺伝子のヌクレオチド配列のすべてまたは一部から成ることを特徴 とする請求の範囲第1項から第6項までのいずれかに記載のベクター。 8.たんぱく毒を暗号化するヌクレオチド配列(6)に制限酵素SmaIの切断 部位(cleavage site)が存在しないことを特徴とする請求の範囲第7項に記載の ベクター。 9.寄託No.LMBP2781に相当することを特徴とする請求の範囲第1項 から第8項までのいずれかに記載のベクター。 10.請求の範囲第1項から第9項までのいずれかに記載のクローニングベクタ ーで形質転換される原核細胞。 11.好ましくは染色体Iq及び高い形質転換効率を有し、融合たんぱくの毒性 に対する耐性を授ける突然変異を有し、及び/または融合たんぱくに対する抗毒 素であるたんぱくを暗号化する遺伝子を有することを特徴とする請求の範囲第1 項から第9項までのいずれかに記載のクローニングベクターのための原核宿主細 胞。 12.ジャイレースのサブユニットAを暗号化する遺伝子またはジャイレースの サブユニットBを暗号化する遺伝子中に突然 変異を有し、融合たんぱくに対する耐性を授け、及び/または融合たんぱくに対 する抗毒素であるたんぱくCcdAを暗号化する遺伝子を有することを特徴とす る請求の範囲第11項に記載のクローニングベクターのための原核宿主細胞。 13.ジャイレースのGyrAポリペプチドのアミノ酸配列中でアルギニン46 2をシステインに代える突然変異を有し、それによって融合たんぱくに対する耐 性を授けることを特徴とする請求の範囲第11項または第12項に記載の大腸菌 細胞。 14.LacIq突然変異を有することを特徴とする請求の範囲第11項から第 13項までのいずれかに記載の細胞。 15.No.LMGP−12601として寄託されていることを特徴とする請求 の範囲第11項から第14項までのいずれかに記載の細胞。 16.いくつかの特定クローニング部位を含むヌクレオチド配列(5)の両側に 位置する配列とハイブリッド化する10ないし30個のヌクレオチドの配列から 成ることを特徴とする請求の範囲第1項から第9項までのいずれかに記載のベク ターの断片。 17.組換えクローンの選択を目的とする請求の範囲第1項から第9項までのい ずれかに記載のクローニングベクターの利用。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BE9200696A BE1006085A3 (fr) | 1992-07-31 | 1992-07-31 | Vecteur de clonage. |
BE9200696 | 1992-07-31 | ||
PCT/BE1993/000051 WO1994003616A2 (fr) | 1992-07-31 | 1993-08-02 | Vecteur de clonage et/ou de sequençage |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH08500484A true JPH08500484A (ja) | 1996-01-23 |
JP3654648B2 JP3654648B2 (ja) | 2005-06-02 |
Family
ID=3886385
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP50484094A Expired - Lifetime JP3654648B2 (ja) | 1992-07-31 | 1993-08-02 | クローニング及び/または配列ベクター |
Country Status (8)
Country | Link |
---|---|
US (3) | US5910438A (ja) |
EP (1) | EP0652963B1 (ja) |
JP (1) | JP3654648B2 (ja) |
AU (1) | AU4553093A (ja) |
BE (1) | BE1006085A3 (ja) |
CA (1) | CA2141412A1 (ja) |
DE (1) | DE69314180T2 (ja) |
WO (1) | WO1994003616A2 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004527238A (ja) * | 2001-02-23 | 2004-09-09 | ユニベルスィテ リーブル ドゥ ブリュッセル | 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法 |
Families Citing this family (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BE1006085A3 (fr) * | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
US7176029B2 (en) * | 1992-07-31 | 2007-02-13 | Universite Libre De Bruxelles | Cloning and/or sequencing vector |
US6964861B1 (en) | 1998-11-13 | 2005-11-15 | Invitrogen Corporation | Enhanced in vitro recombinational cloning of using ribosomal proteins |
US6720140B1 (en) * | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
CA2226463A1 (en) | 1995-06-07 | 1996-12-19 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
US5851808A (en) | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
JP4303418B2 (ja) * | 1997-10-24 | 2009-07-29 | ライフ テクノロジーズ コーポレーション | 組換え部位を有する核酸を使用する組換えクローニング |
CN101125873A (zh) * | 1997-10-24 | 2008-02-20 | 茵维特罗根公司 | 利用具重组位点的核酸进行重组克隆 |
US6022716A (en) | 1998-04-10 | 2000-02-08 | Genset Sa | High throughput DNA sequencing vector |
CA2327387C (en) * | 1998-05-07 | 2012-07-03 | Gx Biosystems A/S | Cytotoxin-based biological containment |
EP1159402A4 (en) * | 1999-03-02 | 2003-01-29 | Invitrogen Corp | CELLS RESISTANT TO TOXIC GENES AND THEIR USE |
DE60042969D1 (de) | 1999-03-02 | 2009-10-29 | Life Technologies Corp | Zubereitungen und methoden zur verwendung in rekombinatorischem klonen von nukleinsäuren |
ATE391789T1 (de) * | 1999-12-10 | 2008-04-15 | Invitrogen Corp | Verwendung einer vielzahl von rekombinationsstellen mit einzigartiger spezifität beim rekombinatorischen klonen |
EP1111061A1 (en) * | 1999-12-20 | 2001-06-27 | Universite Libre De Bruxelles | Double selection vector |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US6544782B1 (en) * | 2000-11-13 | 2003-04-08 | Synthegen Systems | pREM: a positive selection vector system for direct PCR cloning |
US20050260585A1 (en) * | 2002-03-19 | 2005-11-24 | Cedric Szpirer | Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms |
TWI259011B (en) * | 2002-04-12 | 2006-07-21 | Interdigital Tech Corp | Access burst detector correlator pool |
WO2004009768A2 (en) * | 2002-07-18 | 2004-01-29 | Invitrogen Corporation | Viral vectors containing recombination sites |
JP4336770B2 (ja) * | 2002-07-26 | 2009-09-30 | 独立行政法人産業技術総合研究所 | 致死遺伝子を用いた形質転換体選択用マーカー |
US9309518B2 (en) | 2002-09-03 | 2016-04-12 | Universite Libre De Bruxelles | Reversible, parallel and multitask cloning method and kit |
CA2496514C (en) * | 2002-09-03 | 2012-11-06 | Universite Libre De Bruxelles | Reversible, parallel and multitask cloning method and kit |
EP1644538A4 (en) * | 2003-06-26 | 2006-11-08 | Invitrogen Corp | METHODS AND COMPOSITIONS FOR DETECTING PROMOTER ACTIVITY AND EXPRESSING FUSION PROTEINS |
US8293503B2 (en) * | 2003-10-03 | 2012-10-23 | Promega Corporation | Vectors for directional cloning |
JP5089984B2 (ja) * | 2003-10-03 | 2012-12-05 | プロメガ コーポレイション | 方向性クローニング用ベクター |
EP1697534B1 (en) | 2003-12-01 | 2010-06-02 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
EP2119789A1 (en) | 2008-05-16 | 2009-11-18 | Université Libre de Bruxelles | Hyperproliferative recombinant cell |
KR101077942B1 (ko) | 2009-02-05 | 2011-10-31 | 서울대학교산학협력단 | 식물체 일시 발현용 고처리량 벡터 및 그 용도 |
HUE033858T2 (en) | 2012-02-14 | 2018-01-29 | Merial Inc | Rotavirus unit vaccines and method for their preparation and use |
US10358655B1 (en) | 2012-06-27 | 2019-07-23 | University Of Utah Research Foundation | Attenuated protein expression vectors and associated methods |
CN105658070B (zh) | 2013-08-19 | 2019-10-18 | 辛格隆股份公司 | 微生物的受控生长 |
US11618899B2 (en) | 2016-02-09 | 2023-04-04 | Brookhaven Science Associates, Llc | Cloning and expression vectors and systems |
WO2019046577A1 (en) | 2017-08-31 | 2019-03-07 | Syngulon Sa | METHODS AND COMPOSITIONS FOR THE PRODUCTION OF BACTERIOCIN AND ANTIMICROBIAL PEPTIDES |
US11932672B2 (en) | 2017-12-19 | 2024-03-19 | Syngulon S.A. | Fermentation process |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0273040B1 (en) * | 1986-03-26 | 1994-06-22 | GX BioSystems A/S | Biological containment |
US5464764A (en) * | 1989-08-22 | 1995-11-07 | University Of Utah Research Foundation | Positive-negative selection methods and vectors |
US5300431A (en) * | 1991-02-26 | 1994-04-05 | E. I. Du Pont De Nemours And Company | Positive selection vector for the bacteriophage P1 cloning system |
US7176029B2 (en) * | 1992-07-31 | 2007-02-13 | Universite Libre De Bruxelles | Cloning and/or sequencing vector |
BE1006085A3 (fr) * | 1992-07-31 | 1994-05-10 | Univ Bruxelles | Vecteur de clonage. |
JP2590761B2 (ja) * | 1994-11-22 | 1997-03-12 | 日本電気株式会社 | Tab式半導体装置およびtab式半導体装置を回路基板に接続する方法 |
US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
CA2226463A1 (en) * | 1995-06-07 | 1996-12-19 | Life Technologies, Inc. | Recombinational cloning using engineered recombination sites |
US5922583A (en) * | 1995-10-17 | 1999-07-13 | Biostar Inc. | Methods for production of recombinant plasmids |
CA2327387C (en) | 1998-05-07 | 2012-07-03 | Gx Biosystems A/S | Cytotoxin-based biological containment |
US6271359B1 (en) * | 1999-04-14 | 2001-08-07 | Musc Foundation For Research Development | Tissue-specific and pathogen-specific toxic agents and ribozymes |
CA2435956C (en) * | 2001-02-23 | 2012-07-10 | Universite Libre De Bruxelles | Method for the selection of recombinant clones comprising a sequence encoding an antidote protein to toxic molecule |
US20050260585A1 (en) * | 2002-03-19 | 2005-11-24 | Cedric Szpirer | Poison/antidote genetic systems for the selection of genetically modified eucaryote cells or organisms |
-
1992
- 1992-07-31 BE BE9200696A patent/BE1006085A3/fr not_active IP Right Cessation
-
1993
- 1993-08-02 CA CA002141412A patent/CA2141412A1/en not_active Abandoned
- 1993-08-02 US US08/379,614 patent/US5910438A/en not_active Expired - Lifetime
- 1993-08-02 JP JP50484094A patent/JP3654648B2/ja not_active Expired - Lifetime
- 1993-08-02 EP EP93915577A patent/EP0652963B1/fr not_active Expired - Lifetime
- 1993-08-02 WO PCT/BE1993/000051 patent/WO1994003616A2/fr active IP Right Grant
- 1993-08-02 AU AU45530/93A patent/AU4553093A/en not_active Abandoned
- 1993-08-02 DE DE69314180T patent/DE69314180T2/de not_active Expired - Lifetime
-
1999
- 1999-01-04 US US09/225,152 patent/US6180407B1/en not_active Expired - Lifetime
-
2007
- 2007-08-20 US US11/842,131 patent/US20080299661A1/en not_active Abandoned
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2004527238A (ja) * | 2001-02-23 | 2004-09-09 | ユニベルスィテ リーブル ドゥ ブリュッセル | 毒性分子に対する抗毒性蛋白質をコードする配列を含む組換体クローンの選択方法 |
Also Published As
Publication number | Publication date |
---|---|
JP3654648B2 (ja) | 2005-06-02 |
BE1006085A3 (fr) | 1994-05-10 |
US6180407B1 (en) | 2001-01-30 |
EP0652963A1 (fr) | 1995-05-17 |
DE69314180T2 (de) | 1998-03-19 |
DE69314180D1 (de) | 1997-10-30 |
AU4553093A (en) | 1994-03-03 |
US20080299661A1 (en) | 2008-12-04 |
WO1994003616A3 (fr) | 1994-03-31 |
CA2141412A1 (en) | 1994-02-17 |
WO1994003616A2 (fr) | 1994-02-17 |
EP0652963B1 (fr) | 1997-09-24 |
US5910438A (en) | 1999-06-08 |
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