JPH0825865B2 - Cosmetics for preventing skin aging - Google Patents

Description

TECHNICAL FIELD The present invention relates to cosmetics for preventing skin aging. More specifically, the skin aging exhibiting an excellent skin aging preventive effect, which is obtained by blending dried powder of Gymnema sylvestre and at least one component of an extract obtained from dried leaves of Gymnema sylvestre as a base for cosmetics for skin. Related to preventive cosmetics.

[Prior Art] Generally, when the skin ages, it loses its flexibility and elasticity.
Wrinkles increase, resulting in dry and rough skin. Various cosmetic raw materials such as moisturizers, cell activators, ultraviolet absorbers, and antioxidants have been developed for the purpose of preventing these, and cosmetics containing these cosmetic raw materials for preventing rough skin are It is of great interest to general consumers.

Various factors have been considered for skin aging, and among them, lipid peroxide due to oxidation of unsaturated fatty acids in sebum has been mentioned as a particularly large factor. Lipid peroxide is a source of cell damage such as denaturation of proteins and oxygen.

The skin of the human body is the outermost layer of the body and is more exposed to light than any other organ. In addition, sebum contains a large amount of unsaturated fatty acids, which results in the formation of lipid peroxides. It is easy to be done. Conventionally, in order to prevent the peroxidation of sebum, UV absorbers such as para-aminobenzoates and antioxidants such as tocopherol, cysteine, and radical scavengers such as glutathione have been used, but in small amounts The preventive effect cannot be expected, and if used in a large amount, it may deteriorate due to light (decrease in efficacy, discoloration or odor unsuitable for cosmetics), or adverse side effects on the skin, and so on. Was still not enough.

[Problems to be Solved by the Invention] As a result of intensive studies conducted by the present inventors in view of the present situation, an extract of Gymnema sylvestre has a remarkable effect of suppressing the peroxidation of biological lipids, and is safe for the skin. It was found that the property is high. After further diligent research,
It has been found that a cosmetic containing such an extract has an excellent effect on preventing rough skin and preventing aging, and has completed the present invention.

Means for Solving the Problem That is, the present invention is characterized by containing powder of dried leaves of Gymnema sylvestre and at least one component of an extract obtained from dried leaves of Gymnema sylvestre, which is characterized by skin aging. Related to preventive cosmetics.

[Actions and Examples] Gymnema sylvestre used in the present invention is a plant belonging to the potato family of Indian origin, and its hot water extract has a sweetness suppressing effect and a sugar absorption suppressing effect in the intestinal tract,
In India, it is used as a crude drug for the treatment of diabetes.

Gymnemic acid is known as an active ingredient showing these suppressing effects. Gymnemaic acid has homologues of A _{1 to} A ₄ , and its basic structure is a glycoside consisting of a derivative of gymnemagenin, which is a triterpene, and D-glucuronic acid.

The dried leaf powder of Gymnema sylvestre used in the present invention may be a powder that passes through a sieve of about 80 mesh after pulverization. Further, as an extract obtained by extracting from dried leaves of Gymnema sylvestre (hereinafter referred to as Gymnema sylvestre extract), for example, an extract obtained by extracting dried leaves of Gymnema sylvestre into water, hot water, aqueous alcohol, etc., Examples thereof include a concentrated extract obtained by concentrating the extract, and a solvent solution of the concentrated extract obtained by dissolving the concentrated extract in a solvent such as 1,3-butylene glycol.

There are various methods for obtaining the extract of Gymnema sylvestre, and one example of the method is, for example, (a) dried leaves of Gymnema sylvestre in the state as it is or if necessary, 100 parts of distilled water (weight) Part, the same as below), and a method of obtaining a hot water extract of Gymnema sylvesta by boiling for 5 to 6 hours while appropriately supplementing distilled water so that the water content does not decrease, (b) Gymnema Sylvester dried leaves are left as they are or if necessary, they are chopped and mixed in an amount of 1 to 10 parts with 100 parts of an aqueous alcoholic solution such as 30 to 60% of ethanol and left for about 7 to 20 days, for example, at room temperature. Examples thereof include a method of obtaining a Gymnema sylvestre extract of hydroalcohol by filtering by suction filtration or the like, but the present invention is not limited to these methods. Not a thing.

As a method of obtaining a concentrated extract from the extract of Gymnema sylvestre, for example, (a) a method of concentrating the extract by a vacuum distillation method, (b) a method of concentrating the extract by a freeze-drying method, (c) an extraction Examples of the method include a method of concentrating the liquid by a spray drying method, but the present invention is not limited to these methods. Since the hot water extract of Gymnema sylvestre has a very large foaming property, it is preferable to concentrate it by the freeze-drying method or the spray-drying method. There is no particular limitation on the concentration of the extract in the concentrated extract, and it is preferable to appropriately adjust the concentration according to the purpose, application, etc.

The solvent solution of the concentrated extract is obtained by dissolving the concentrated extract in a solvent such as 1,3-butylene glycol, propylene glycol, glycerin, or aqueous ethanol, and the concentration of the extract in the solvent solution is concentrated extraction. It is preferably adjusted appropriately according to the purpose and use of the product.

The cosmetic for preventing skin aging of the present invention can be obtained by appropriately blending at least one component of the dried powder of Gymnema sylvestre thus obtained and the extract of the dried leaf with a cosmetic ingredient.

In the cosmetic for preventing skin aging of the present invention, the content of at least one component of the dried leaf of Gymnema sylvestre and the extract of Gymnema sylvestre in the dried leaf of Gymnema sylvestre is usually 0.025 to 50%, preferably 0.25.
~ 25%. When the content is less than the above range, the effect due to the combination of dried leaves of Gymnema sylvestre and Gymnema sylvestre extract is reduced, and when it exceeds the above range, in the production process, for example, precipitates. And separation tends to occur.

Moreover, the dosage form of the cosmetics of the present invention is arbitrary, and examples thereof include solubilizing systems such as lotion, emulsions such as emulsions and creams, ointments, powder dispersion systems, water-oil two-layer systems, water-oil-powder. Three
Any formulation such as a layer system may be used.

Examples of the cosmetic ingredients used in the cosmetics of the present invention include excipients, fragrances, coloring materials, active ingredients and the like.

Examples of the excipients include oils and fats such as olive oil, beeswax, lanolin, candelilla wax, squalane, paraffin, stearic acid, cetanol, octyldodecyl myristate, tri (caprylic capric acid) glycerin; sodium alginate, dextrin,
Water-soluble polymer compounds such as sodium carboxymethylcellulose, carboxyvinyl polymer and polyvinyl alcohol; polyhydric alcohols such as propylene glycol and polyethylene glycol; sugars such as glucose, sorbitol and maltitol; sorbitan monostearate ester and polyoxyethylene Examples thereof include surfactants such as glycerin monostearate polyoxyethylene nonylphenyl ether, and these components may be used alone or in admixture of two or more.

Examples of the coloring material include tar-based pigments such as red No. 2 and blue No. 1, and inorganic pigments such as talc, mica and titanium oxide.

The active ingredient is appropriately selected according to the use of the cosmetic of the present invention. Specific examples of the active ingredient include vitamins such as vitamin E, vitamin C and vitamin D; licorice extract, Aloe extracts, animal and plant extract components such as placenta; antioxidants such as dibutylhydroxytoluene; antiplasmin agents such as epsilon aminocaproic acid and tranexamic acid; and ultraviolet absorbers such as paramethoxycinnamic acid ethylhexyl ester and oxybenzone.

The blending amount of each of these cosmetic ingredients varies depending on the intended cosmetic product and cannot be determined in a general manner, so it is preferably adjusted appropriately according to such applications.

The cosmetic for preventing skin aging of the present invention thus obtained is
It is a mixture of Gymnema sylvestre extract, which has a remarkable effect in suppressing the peroxidation of biological lipids and is safe for the human body, and has excellent properties such as prevention of rough skin and prevention of aging.

Next, the cosmetic for preventing skin aging of the present invention will be described in more detail based on Production Examples, Comparative Production Examples, Examples and Comparative Examples, but the present invention is not limited to these Production Examples and Examples. .

Production Example 1 20 g of dried leaves of Gymnema sylvestre was added to 300 ml of distilled water,
Boil for 5 to 6 hours, add water if the water evaporates,
Finally, 300 ml of extract was obtained. After the extract was allowed to cool, it was suctioned a little at a time, and the solution was freeze-dried to obtain about 4 g (yield 20%) of a hot water extract.

Production Example 2 20 g of dried leaves of Gymnema sylvestre were immersed in 500 ml of 50% aqueous ethanol solution. The mixture was allowed to stand at room temperature, and after 7 days, it was suctioned to obtain an extract. The extract was distilled off under reduced pressure at about 30 ° C. to obtain about 6 g (yield 30%) of extract.

Reference Examples 1 to 6 Wistar rats (male, weight 200 to 300)
g) was slaughtered, and the liver was perfused with physiological saline, then extracted, and a 10% hepatocyte homogenate solution was prepared using 1.15% potassium chloride aqueous solution. Using.

Gymnema sylvestre hot water extract or Gymnema sylvestre 50% obtained in Production Example 1 or 2 as a test substance
Using ethanol extract, add it at the concentration shown in Table 1, and add ultraviolet rays (Toshiba Blacklight FL40SBLB; 6 lights,
Irradiation energy: 390 × 10 ⁸ erg). 0.1 ml of each hepatocyte homogenate solution before and 4 hours after irradiation was sampled. Next, the amount of lipid peroxide in the sampled hepatocyte homogenate solution was determined by the TBA method shown below, and the lipid peroxide inhibition rate of the test substance was calculated by the following formula. The results are shown in Table 1.

(TBA method) 0.1% of 10% hepatocyte homogenate solution, 8.1% SLS
(Sodium lauryl sulphate) aqueous solution 0.2 ml, 20% acetate buffer (pH 3.5) 1.5 ml, 0.8% TBA (thiobarbituric acid) 1.5 ml and distilled water 0.7 ml Heat for hours.

After cooling with running water, add 1 ml of distilled water. further,
After adding 5.0 ml of a mixed solution (15: 1) of n-butanol and pyridine and stirring sufficiently, centrifugation is performed at 3000 rpm for 15 minutes.

The absorbance of the n-butanol-pyridine layer is measured at a wavelength of 532 nm.

(In the formula, D _SO is T cell of the hepatocyte homogenate solution before UV irradiation.
Absorbance after treatment by the BA method, D _S is the absorbance after treatment of the hepatocyte homogenate solution after UV irradiation by the TBA method, and D _TO is the hepatocyte homogenate solution after addition of the test substance before UV irradiation by the TBA method. Absorbance after treatment, D _T
Shows the absorption luminosity after irradiating the hepatocyte homogenate solution to which the test substance was added with ultraviolet rays and then treating with the TBA method. ) Reference Example 7 The backs of three Japanese white improved rabbits per group were clipped with clippers. Next, 0.1 ml each of the hot water extract of Gymnema sylvestre obtained in Production Example 1 or Production Example 2 or a 1% aqueous solution of 50% ethanol extract was applied to a tory bond (regular size) and applied to the cut portion. .

24 hours and 72 hours after application, the skin irritation was examined by the following Draize criterion. Table 2 shows the results.

[Evaluation Criteria for Primary Skin Irritation (Draize Criteria)] Erythema and crusting 0: No erythema 1: Very slight erythema (finally noticeable) 2: Clear erythema 3: Moderate to strong Erythema 4: Strongly reddish erythema with mild crusting (injury deep) Edema formation 0: No edema 1: Very slight edema (finally noticeable) 2: Clear edema (clearly distinguishable from surroundings) 3: Moderate edema (raised by about 1 mm) 4: Strong edema (raised by 1 mm or more and spread around) From the results shown in Table 2, it can be seen that the extract of Gymnema sylvestre has no primary skin irritation.

Reference Example 8 20 ml / kg (dry matter: 9.8 g / kg) of a 50% aqueous solution of the Gymnema sylvestre hot water extract obtained in Production Example 1 was added to a ddY strain mouse (male and female, 4 to 5 weeks old, weight: 20 to 30 g). After oral administration, symptoms were observed for 7 days after the administration.

Next, ddY mice (male and female 6 to 7 weeks old, body weight 30 to 40 g)
An 8% aqueous solution of Gymnema sylvestre hot water extract obtained in the same manner as in Production Example 1 was intraperitoneally administered at 12.5 ml / kg (1 g / kg for a dried product), and symptoms were observed for 7 days after the administration.

As a result, as shown in Table 3, no mortality was observed by oral administration even when the dose was 9.8 g / kg.
Even with subcutaneous administration, there was no death at 1.0 g / kg. Other,
No abnormalities were observed in general symptoms in the mice administered by any of the administration routes for 7 days. Therefore, it is understood that the Gymnema sylvestre hot water extract used in the present invention is highly safe.

Example 1 [Lotion] (Component) (parts by weight) Glycerin 4.0 1,3 Butylene glycol 5.0 Polyoxyethylene hydrogenated castor oil (30E.O) 3.0 Ethanol 10.0 Gymnema sylvestre hot water extract 1.0 Methyl paraoxybenzoate 0.1 Perfume 0.1 Purified water 76.8 (Note) “30E.O” is the number of moles of ethylene oxide added 3
It means 0 mole.

A lotion for preventing skin aging was prepared by mixing the above components so as to have a uniform composition.

Example 2 [Pack] (Component) (parts by weight) Polyvinyl alcohol 13.0 Propylene glycol 5.0 Glycerin 3.0 Ethanol 6.0 Polyoxyethylene hydrogenated castor oil (30E.O.) 0.5 Titanium oxide 7.0 Kaolin 5.0 Gymnema sylvestre dry leaf powder 3.0 Perfume 0.1 Paraoxy Methyl benzoate 0.1 Purified water 59.3 The above components were mixed before a uniform composition to prepare a pack for preventing skin aging.

Example 3 [emollient cream] (component) (parts by weight) Liquid paraffin 7.0 Stearic acid 3.0 Cetanol 4.5 Isopropyl myristate 5.0 Polyoxyethylene cetyl ether (25E.O.) 2.5 Lipophilic glyceryl monostearate 2.0 Propylene glycol 5.0 sorbit Liquid (65%) 8.0 Gymnema sylvestre 50% Ethanol extract 1.5 Methyl paraoxybenzoate 0.1 Perfume 0.1 Purified water 61.3 The above components were mixed to give a uniform composition to prepare an emollient cream for preventing skin aging.

Example 4 [Milk lotion] (Component) (parts by weight) Stearic acid 2.0 Cetanol 2.0 Squalane 15.0 Lipophilic glyceryl monostearate 1.0 1,3 Butylene glycol 3.0 Sodium hydroxide 0.3 Gymnema sylvesta hot water extract 2.0 Methyl paraoxybenzoate 0.1 Cinoxate 1.0 Fragrance 0.1 Purified water 73.5 The above components were mixed so as to have a uniform composition to prepare a milk lotion for preventing skin aging.

Comparative Example 1 A milk lotion was prepared in the same manner as in Example 4, except that 2.0 parts by weight of purified water was used instead of the Gymnema sylvestre hot water extract.

Next, a use test was performed on the milk lotions obtained in Example 4 and Comparative Example 1. Table 4 shows the results.

 The usage test method is as follows.

(Test method of use) The milk lotion of Example 4 and the milk lotion of Comparative Example 1 were applied to the back of the hand twice a day for 2 months and applied to 25 middle-aged female panelists with rough skin on the back of the hand. As the skin condition before the start and after the end, the rough skin and the swelling were evaluated based on the following criteria.

(Rough skin) Effective: There is an improvement effect on dry scales, etc. Slightly effective: Improvement on dry scales, etc. There is a little hardening. Ineffective: No improvement effect on dry scales, etc. (Burning) Effective: There is an improving effect on skin scalp. Improving cure for acupuncture Somewhat ineffective: No improving effect on skin acuity From the results in Table 4, it can be seen that the addition of Gymnema sylvestre extract exerts an excellent effect on symptoms related to aging such as rough skin and acupuncture.

[Effect of the Invention] The cosmetic of the present invention is a mixture of dried powder of Gymnema sylvestre and at least one component of the extract extracted from dried leaf of Gymnema sylvestre as an active ingredient of skin cosmetics. Since it suppresses peroxidation, it can prevent aging of the skin, and is also excellent in safety for the skin.

Claims (1)

[Claims] 1. A cosmetic for preventing skin aging, which contains powder of dried leaves of Gymnema sylvestre and at least one component of an extract obtained by extracting from dried leaves of Gymnema sylvestre.