JP3010560B2 - Cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a novel cosmetic. For more information,
The present invention relates to a cosmetic having a whitening effect, an anti-inflammatory effect, and a water-retaining ability, which contains a water-soluble extract of yugao fruit as an active ingredient.

[Prior art] Although there are unknown points about the mechanism of occurrence of skin spots, freckles, etc., in general, melanin pigments are formed due to abnormal hormones and the stimulation of ultraviolet rays from sunlight.
It is believed that this is abnormally deposited in the skin. For the treatment of such spots and freckles, substances that inhibit tyrosinase activity present in the skin and suppress melanin production, for example, a method of administering a large amount of vitamin C, glutathione to ointments, creams, lotions, etc. A method of applying the composition in a form and applying it locally is used. In the United States and Europe, hydroquinone preparations are used as pharmaceuticals.

In addition, various skin irritation, for example, the skin is irritated by the action of ultraviolet rays, and those that suppress these inflammations are anti-inflammatory agents, and as cosmetic ingredients, plant extracts such as aloe and loofah Glycyrrhizin, zinc white and the like are used.

In addition, a decrease in the water content from the stratum corneum of the skin causes skin roughness and the like. To give the stratum corneum an appropriate moisture content,
Glycerin, 1,3-butylene glycol, propylene glycols, hyaluronic acid and the like are used as humectants.

It should be noted that as a general use method of yugao, it is mainly used for the production of ginger in the food field, but there is no record of use as a cosmetic.

[Problems to be Solved by the Invention] Vitamin Cs have poor temporal stability to heat and light.
In particular, it causes discoloration and odor in a system containing water. on the other hand,
The use of hydroquinones is restricted due to safety problems such as skin irritation and allergies. In addition, there is a problem in terms of stability because the air is easily oxidized.
Thiol compounds such as glutathione and cysteine have a strong off-flavor, are easily oxidized, and have a slow effect. Also, 2
-Mercaptoethylamine salt, N- (2-mercaptoethyl) dimethylamine salt and the like are known to decolorize the skin of black guinea pigs, but are not generally used because vitiligo tends to occur after decolorization.

On the other hand, as described above, there are various components having a whitening effect, an anti-inflammatory effect, and a moisturizing effect. However, in order to produce a cosmetic having these effects, a plurality of these components must be added. In many cases, attention must be paid to product stability.

[Means for Solving the Problems] In view of such circumstances, the present inventors have conducted intensive studies and as a result, have found that a cosmetic containing a water-soluble extract of yugao fruit as an active ingredient has a favorable effect. The present inventors have found that they exhibit a whitening effect, an anti-inflammatory effect and a water retention effect, and have completed the present invention.

The present invention is a cosmetic containing a water-soluble extract of the fruit of Yugao as an active ingredient.

Yugao used in the present invention is a genus Oribataceae, a genus Hypogalus, or Hyugao, whose scientific name is Lagenaria leucantha Rusby ve.
It is called r.ciauat Makino (the cited reference is the primary color Makino Plant Encyclopedia; Hokuryukan), for example, cultivated products can be used.

The fruit of the plant of the present invention is extracted with a water-soluble solvent, and examples thereof include solvents such as water, alcohols (eg, methanol, ethanol, 1,3-butylene glycol, propylene glycol), and acetone. Extraction may be carried out using one or more of these water-soluble solvents. Further, it may be one extracted by heating or one extracted at normal temperature. If necessary, it can be concentrated or diluted and used as a cosmetic raw material.

The cosmetics of the present invention include oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, metal soaps, and surfactants within a range that does not impair the effect of the water-soluble extract of yugao fruit. Etc. can be blended as a raw material.

Examples of cosmetics produced using these base materials as raw materials include lotions, creams, emulsions, shampoos, foundations, lip balms, lipsticks, and the like.

The amount of the water-soluble extract of the fruit of the seaweed used for the cosmetic of the present invention, as a solid obtained by distilling off the solvent,
0.01 to 10% by weight, preferably 0.1%
It is appropriate to mix it by 5.0% by weight. If it is less than 0.01% by weight, a sufficient effect cannot be expected, and if it exceeds 10% by weight, the effect is not enhanced and it is uneconomical.

Also, regarding the method of addition, even if it is added in advance,
It may be added during the production, and may be appropriately selected in consideration of workability.

Hereinafter, the present invention will be described more specifically with reference to Examples, but the present invention is not limited thereto. In addition, the part shown in an Example shows a weight part, and% shows weight%.

[Examples] Example 1 500 g of a fruit of Yugao is shredded, heat-extracted twice with 500 ml of water for 2 hours each, and further concentrated by vacuum freeze-drying to obtain 1 g of the extract (containing a solid content of 99% or more). I got

Example 2 After thoroughly washing 100 g of Campyo (commercial product) with water,
The mixture was heated and extracted twice with ml for 2 hours. After being filtered off and remaining, the filtrate was concentrated under reduced pressure, further freeze-dried in vacuo, and extracted 6 g.
(Containing 99% or more solids).

Example 3 60 g of dried yugao fruit was pulverized, heated and extracted with 600 ml of a water-ethanol mixture (1: 1) for 5 hours, and further concentrated to obtain 2 g of the extract (containing 50% solids). Was.

Example 4 60 g of dried yugao fruit was ground and mixed with 300 parts of ethanol.
Add ml and leave at room temperature for one month. Further concentration yielded 1 g of extract (containing 99% or more solids).

Example 5 60 g of dried yugao fruit was ground and mixed with propanol 6
The mixture was extracted by heating twice with 00 ml each for 2 hours, and further concentrated to obtain 1 g of the extract (containing 70% solids).

Example 6 Lotion Hot water extract of the fruit of the seaweed (99% as a solid) 5.0 parts Glycerin 2.0 Ethyl alcohol 7.0 Methyl parahydroxybenzoate 0.05 Polyoxyethylene (20) lauryl ether 0.5 Citric acid 0.01 Sodium citrate 0.1 Purified water 85.34 [Production Method] Ingredients (1) and (2) are mixed and dissolved. Separately ingredients ~
And dissolve. Then, both are mixed and filtered through a Tetron cloth (300 mesh) to obtain a product.

Example 7 Lotion Hot water extract of camphor (99% as a solid) 2.0 parts Glycerin 2.0 Ethyl alcohol 7.0 Polyoxyethylene (20) lauryl ether 0.5 Methyl parahydroxybenzoate 0.05 Citric acid 0.01 Sodium citrate 0.1 Purified water 85.34 [ Production method] The components (1) and (2) are mixed and dissolved. Separately ingredients ~
And dissolve. Then, both are mixed and filtered through a Tetron cloth (300 mesh) to obtain a product.

Example 8 Hot water extract of cream melon fruit (99% as solid) 2.0 parts Squalane 5.5 Olive oil 3.0 Stearic acid 2.0 Beeswax 2.0 Octyldodecyl myristate 3.5 Polyoxyethylene (20) cetyl ether 3.0 Behenyl alcohol 1.5 Glycerin monostearate 2.5 1,3-butylene glycol 8.5 Methyl paraoxybenzoate 0.2 Ethyl paraoxybenzoate 0.05 Fragrance 0.1 Purified water 66.15 [Preparation method] Heat and dissolve ingredients and mix to keep the oil at 70 ° C to obtain an oil phase. The components (1) to (4) are dissolved in the components by heating and mixed, and the mixture is kept at 75 ° C. to form an aqueous phase. Add the water phase to the oil phase and emulsify. Add the ingredients and stir while cooling to 30 ° C to obtain the product.

Example 9 Emulsion Ethanol extract of the fruit of the seaweed (99% as a solid)
1.0) squalane 5.0 olive oil 5.0 jojoba oil 5.0 cetyl alcohol 1.5 glycerin monostearate 2.0 polyoxyethylene (20) cetyl ether 3.0 polyoxyethylene (20) sorbitan monooleate 2.0 dipropylene glycol 1.0 glycerin 2.0 fragrance 0.1 paraoxybenzoic acid Methyl 0.2 Purified water 71.12 [Preparation method] Heat and dissolve ingredients and mix, keep at 70 ° C to obtain oil phase. The components are dissolved in the components by heating and mixed, and the mixture is kept at 75 ° C. to form an aqueous phase. Add the water phase to the oil phase, emulsify and disperse, cool to 30 ° C while adding the components and stirring.

EXAMPLE 10 Propylene Glycol Extract of Pack Yugao Fruit (20% as a solid) 3.0 parts Glycerin 11.5 1,3-butylene glycol 2.5 Polyoxyethylene (40) hydrogenated castor oil 1.0 Citric acid 0.1 Sodium citrate 0.3 Paraoxybenzoic acid Methyl 0.2 Flavor 0.05 Purified water 70.85 [Production method] Ingredients (1) and (2) are uniformly mixed and dissolved to give a product.

[Effect of the Invention] The cosmetic containing the water-soluble extract of the fruit of Yugao as an active ingredient of the present invention has a highly stable whitening action, an anti-inflammatory action, and a water retention property, and is also preferable in terms of safety. It is.

 Hereinafter, effects of the present invention will be described with reference to experimental examples.

[Experimental example] Efficacy test example 1 Whitening effect To examine the tyrosinase activity inhibitory effect, 0.15
The tyrosinase activity inhibitory power was measured before and after the heat treatment at 37 ° C. for 2 weeks for the% aqueous solution. For comparison, hot water extracts of ascorbic acid, luffa water, and luffa fruit conventionally used as cosmetics were similarly tested. The sample used was the extract obtained in Example 1.
As a method for preparing a hot water extract of loofah (for comparison), 10 g of a dried product was extracted with hot water (95 ° C., 3 hours, 300 ml),
The filtrate was lyophilized in vacuo.

Measurement of tyrosinase activity inhibitory action; 1 ml of L-tyrosine solution (0.3 mg / ml), 1 ml of Max Bain's buffer (pH 6.8), and 0.9 m of a 0.15% aqueous solution of the sample in a test tube
l was added, and the mixture was incubated in a constant temperature water bath at 37 ° C. for 10 minutes. To this, 0.1 ml of a tyrosinase aqueous solution (1 mg / ml) was added, stirred well, incubated at 37 ° C. for 12 minutes, and set on a spectrophotometer to measure the absorbance at 475 nm.

On the other hand, the same absorbance measurement was performed using distilled water as a blank in place of the sample, and the tyrosinase activity inhibition rate of each sample was calculated by the following equation. In the formula, A means the absorbance when each sample is added, and B means the absorbance of a blank.

Inhibition rate (%) = (1-A / B) × 100 The test results are shown in Table 1. From this table, Example 1
The water-soluble extract of the fruit of Yugao obtained in the above has a remarkable tyrosinase activity inhibitory power than luffa water and hot water extract of luffa, and this composition has good heat stability,
After standing at 37 ° C. for 2 weeks, it is recognized that it has a stronger inhibitory activity on tyrosinase activity than vitamin C. In addition, according to these stability tests, no discoloration or discoloration was observed in the water-soluble extract of the fruit of the gourd. Example 2
When the water-soluble extract of the fruit of the seaweed obtained in Steps (1) to (5) was tested in the same manner, it was found that the extract exhibited the same good inhibitory activity on tyrosinase activity.

Efficacy Test Example 2 Anti-Inflammatory Activity A histamine release inhibition test was performed on aqueous solutions having sample concentrations of 0.01, 0.1, and 1.0%. As a comparison, a luffa water and a hot water extract of a luffa conventionally used in cosmetics were similarly tested. The water-soluble extract of Hyuga, Luffa water and hot water extract of Luffa are the same as those used in Experimental Example 1.

Histamine release inhibition test; report of Hirai et al.
₃₇ , 374, 1983.), the effect of inhibiting histamine release on mast cells collected from the intraperitoneal cavity of male Spraque-Dawley rats (200-450 g) was measured. That is, 4p
The effect of suppressing histamine release by the compound 48/80 of pm was determined as a release inhibition rate (%). Table 2 shows the results. From these results, it can be seen that the hot water extract of the fruit of Yugao obtained in Example 1 has a remarkable inhibitory effect on histamine release and an excellent anti-inflammatory effect as compared with the hot water extract of Kidachi aloe. I found it.

In addition, when the water-soluble extract of the fruit of the seaweed obtained in Examples 2 to 5 was tested in the same manner, it was found that the extract had good anti-inflammatory action.

Effectiveness test example 3 Moisturizing action The water evaporation inhibition rate was measured for the 0.5 and 7.0% aqueous solutions of the samples. As a comparison, glycerin conventionally used in cosmetics as a humectant was similarly tested using distilled water as a blank. The sample used was the hot water extract obtained in Example 1.

Measurement method of water evaporation inhibition rate; 2 ml of each sample solution is placed in a standard bottle, and allowed to stand at a temperature of 25 ° C. and a relative humidity of 50%. After standing for 4 days, the amount of water evaporation of each sample solution was measured, and the water evaporation rate was calculated by the following equation. In the formula, A means the amount of water evaporation of each sample, and B means the amount of water evaporation of the blank (distilled water).

Rejection (%) = (1-A / B) × 100 The test results are shown in Table 3. As is clear from the results, it was found that the hot water extract obtained in Example 1 of the present invention had a water evaporation inhibition rate equivalent to that of glycerin and was superior in water retention capacity.

Efficacy Test Example 4 Use Test A use test was conducted using 30 healthy subjects. As the samples, the cosmetics of Examples 6 and 7 were used, and the weight percentages of the water-soluble extract of the fruit of Gypsophila and the oil of camphor were used. A 2 cm square site inside the subject's forearm
Using a B lamp (Toshiba FL-20SE), ultraviolet rays having an intensity of 3 mW / cm ² were irradiated for 1 minute. After applying each sample to each site twice a day every morning and evening for 3 days, the effect of suppressing inflammation was evaluated by questionnaire survey. A questionnaire survey was also conducted to evaluate the effect of inhibiting pigmentation and the moist feeling of the skin after use for one month. One of the sites irradiated with ultraviolet rays was used as a control in which nothing was applied. The criteria for the questionnaire were evaluated in comparison with the control based on the following.

(Judgment criteria) Valid ◎ Somewhat valid ○ Almost invalid △ Invalid The results shown in Table 4 indicate that the cosmetics used in the present invention show a remarkable effect of suppressing inflammation and pigmentation after sunburn, and also show a good effect in moistening the skin.

Efficacy Test Example 5 Safety Test In order to clarify the safety of the water-soluble extract of the fruit of the gougling of the present invention, a primary irritation test on humans was performed by an occlusion patch test. That is, using a fin chamber (manufactured by EPITEST), 30 healthy people were obstructed and stuck on the flexed side of the forearm for 48 hours, and after 1 hour, 24 hours and 48 hours after removing the patch for patch test The judgment was made using the average value of the judgment. As the sample, the water extract obtained in Example 1 was used, the coating concentration was 10% (w / w) aqueous solution, and purified water was used as a control. As a result, no erythema was observed in the water extract of the fruit of Yugao, while slight erythema was observed in five persons in the purified water of the control. From these results, it was confirmed that the water-soluble extract of Yugao had extremely low primary irritation and was highly safe for the skin. In addition, the water-soluble extract of the fruit of the seaweed obtained in Examples 2 to 5 was tested in the same manner, and it was found that the extract was similarly highly safe for the skin.

──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Masataka Imao 2-130 Tomicho, Nishi-ku, Nagoya-shi, Aichi Japan Menard Cosmetics Co., Ltd.National Research Institute Inspector Naoko Tsukanaka (58) Field surveyed (Int.Cl . ⁷ , DB name) A61K 7/00 A61K 35/78

Claims (1)

(57) [Claims] (1) A cosmetic comprising a water-soluble extract of fruit of yugao.