JP2009143833A - Extract of crude drug and skin external preparation having the same as active ingredient - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an extract of a crude drug, a process for producing an extract of a crude drug and a photoaging inhibition skin external preparation having the obtained extract of a crude drug. <P>SOLUTION: The extract of a crude drug is obtained by extraction of two-stage treatment comprising subjecting a dried product of a crude drug selected from scutellaria root, sophora flower, pueraria root, Gymnema sylvestre, geranium herb, plantago herb, and Areca to pressing wet heat treatment in a pressing wet heat treatment vessel in a saturated water vapor atmosphere of 105°C (19.5 kPaG) to 135°C (211.8 kPaG) and then subjecting the product thus treated to solvent extraction with a polar solvent. The obtained extract of scutellaria root alone or together with the extract from sophora flower, pueraria root, Gymnema sylvestre, geranium herb, plantago herb or areca seed is useful as a photoaging inhibition skin external preparation. <P>COPYRIGHT: (C)2009,JPO&INPIT

Description

  The present invention relates to an extract of a herbal medicine and an extraction method thereof. The present invention also relates to an external preparation for skin containing an extract of herbal medicine as an active ingredient. More specifically, the present invention relates to an external preparation for skin containing as an active ingredient a safe herbal medicine extract having a photoaging inhibitory action.

  The human body is exposed to ultraviolet rays in daily life. UV-A (long wavelength ultraviolet light) and UV-B (medium wavelength ultraviolet light) are ultraviolet rays that affect the skin, and UV-A is an enzyme that damages collagen and elastin fibers that penetrate into the dermis and maintain skin tension. Is increased to cut collagen fibers and alter elastin fibers. For this reason, the skin loses its elasticity and wrinkles and sagging occur. Although UV-B does not reach the dermis, it activates melanocytes in the epidermis to cause sunburn, damage the epidermal cell genes, and cause spots and freckles. Moreover, when active oxygen, lipid peroxide, etc. generate | occur | produce in the epidermis which received ultraviolet rays, these will cause inflammation and will give a serious damage to skin tissue. In addition, these inflammations and skin damage cause rough skin, and when the effect affects the dermis, fibroblasts are damaged, resulting in the destruction of the regenerating function of collagen fibers and elastin fibers, and causes of wrinkles and sagging It becomes. Photoaging refers to skin damage that is difficult to recover due to the influence of ultraviolet rays. Since the degree of photoaging depends on the cumulative amount of ultraviolet irradiation, there are individual differences depending on the living environment and customs up to that point, but ultraviolet irradiation over many years promotes skin aging. Gene wounds that are caused by ultraviolet rays when they are young and accumulate in cells without being repaired may cause adverse effects after many years, such as attracting skin cancer.

In order to prevent and improve such skin photoaging problems, various antioxidants such as naturally-derived α-tocopherol and ascorbic acid have been used (Patent Documents 1 and 2). The effect of preventing and improving the skin troubles caused by this is not sufficient, and attempts to formulate various antioxidant substances extracted from plants into cosmetics, pharmaceuticals, etc. (Patent Documents 3, 4, 5, 6, 7) Due to the difficulty in procuring raw materials, it was difficult to blend an effective amount substantially, such as limited production volume and high manufacturing costs. In addition, as a method for obtaining an extract containing a high concentration of baicalein, which is a useful component in ougon extract, water is added to the chopping of ougon, followed by pretreatment with ougon-degrading enzyme under conditions of 70 ° C. or less, and baicalin Has been proposed (Patent Document 8), but a means for obtaining an extract more efficiently has been desired.
JP-A-8-268862 JP 2001-508809 A Japanese Patent Laid-Open No. 9-175888 JP-A-10-158143 JP 2002-104924 A JP 2002-293738 A JP 2003-192528 A JP 63-27435 A

  An object of the present invention is to obtain a herbal extract excellent in the effect of preventing and improving skin photoaging trouble caused by ultraviolet rays by subjecting herbal medicine to heat and pressure heat treatment and then solvent extraction. The purpose of the present invention is to provide a safe and inexpensive external preparation for photoaging suppression skin based on the excellent effect of preventing and improving the photoaging trouble of the skin of the crude drug extract thus obtained.

  As a result of intensive investigations aimed at developing a safe and inexpensive skin aging inhibitor for skin aging, the present inventors have obtained a dry product of herbal medicine selected from Ogon, Kaika, Kakon, Gymnema Sylvesta, Genokosho, Shazenso, and betel palm. Extraction is carried out in a two-step process of solvent extraction using a polar solvent in a steam and humid heat treatment apparatus under a saturated steam atmosphere of 105 ° C. (19.5 kPaG) to 135 ° C. (211.8 kPaG). Therefore, the extract of herbal medicine can be obtained in extremely high yield, and the ougon extract obtained in this way has excellent photoaging inhibition ability. When used in combination with an extract from genus shochu, such as an extract from geno shochu, which has been conventionally known as an intestinal preparation, It found that the increase in, and have completed the present invention.

  That is, the present invention relates to a dry product of herbal medicine selected from Ogon, Kaika, Kakon, Gymnema sylvestre, Gennosho, Shazenso, and betel wax, in a pressurized moist heat treatment apparatus at 105 ° C. (19.5 kPaG) to 135 ° C. (211.8 kPaG The present invention relates to an extract of herbal medicine obtained by performing a heat and pressure heat treatment in the following saturated water vapor atmosphere and then extracting by a two-stage process of solvent extraction using a polar solvent.

  The present invention also provides a dry product of herbal medicine selected from Ogon, Kaika, Kakon, Gymnema sylvestre, Gennosho, Shazenso, and betel wax, in a pressurized moist heat treatment apparatus at 105 ° C. (19.5 kPaG) or more and 135 ° C. (211.8 kPaG). ) It also relates to a method for producing an extract of herbal medicine, which is extracted by a two-step process of solvent extraction using a polar solvent, followed by pressure and heat treatment in the following saturated water vapor atmosphere.

  Further, according to the present invention, a dried product of Ogon is pressure-humidified and heat-treated in a saturated steam atmosphere of 105 ° C. (19.5 kPaG) or more and 135 ° C. (211.8 kPaG) or less in a pressurized moist heat treatment apparatus, and then a polar solvent is used. The present invention relates to an extract of ougon obtained by extraction by a two-stage process of solvent extraction.

  In the present invention, the dried product of Ogon is pressure-humidified and heat-treated in a saturated steam atmosphere of 105 ° C. (19.5 kPaG) or more and 135 ° C. (211.8 kPaG) or less in a pressure-humidity heat treatment apparatus, The present invention relates to a method for producing an extract of ougon, which is extracted by a two-stage process of solvent extraction to be used.

  In the present invention, the dried product of Ogon is pressure-humidified and heat-treated in a saturated steam atmosphere of 105 ° C. (19.5 kPaG) or more and 135 ° C. (211.8 kPaG) or less in a pressure-humidity heat treatment apparatus, and then a polar solvent is used. The present invention relates to a photoaging-inhibiting skin external preparation containing, as an active ingredient, an extract extracted from argon, which is obtained by extraction through a two-step process of solvent extraction.

Furthermore, in the present invention, a dried product of Ogon is subjected to pressure and humidity heat treatment in a saturated steam atmosphere of 105 ° C. (19.5 kPaG) or more and 135 ° C. (211.8 kPaG) or less in a pressurized moisture heat treatment apparatus, and then the polar solvent is added. Light containing at least one extract selected from the group consisting of Kaika, Kakon, Gymnema sylvestre, Gennosho, Shazenso, and betel lobe, in addition to the extract of Ogon obtained by the two-step process of solvent extraction used It also relates to an anti-aging skin external preparation.
Extracts obtained from herbal medicines such as Kaika, Kakon, Gymnema sylvestre, Gennosho, Shazensou and Areca are used in a saturated water vapor atmosphere of 105 ° C. (19.5 kPaG) or more and 135 ° C. (211.8 kPaG) or less. The extract obtained by the two-stage treatment of the pressure-humidification heat treatment step and the subsequent solvent extraction step may be used, or may be the extract by a conventionally known extraction method that does not employ the pressure-humidification heat treatment step.

  According to the external preparation for photoaging suppression skin of the present invention, it is possible to prevent the photoaging of the skin and to maintain a firm and elastic youthful skin state.

  In the present invention, the extract extracted from ougon uses an extract extracted by a two-stage process of pressurized moist heat treatment in a saturated steam atmosphere at 105 ° C. (19.5 kPaG) or higher and 135 ° C. (211.8 kPaG) or lower, followed by solvent extraction. .

  Here, Ougon is the dried root of the periwinkle of the perennial grass, which is said to have anti-inflammatory, anti-inflammatory, astringent, moisturizing, cell activation, anti-allergic, antibacterial, acne prevention, etc. ing. Its main component is a flavonoid which is a flavone glycoside of polyphenols such as ogonin and baicalin, and baicalin is decomposed into baicalein and glucuronic acid by enzymes in vivo. Baicalein is particularly useful for medicines and the like, and extracts containing much baicalein are prized.

(Pressurized wet heat treatment process in saturated steam atmosphere)
The crude drug product is shredded and crushed as necessary, and accommodated in a pressurized moist heat treatment device, followed by pressurized moist heat treatment in a saturated steam atmosphere. There are no particular limitations on the heat and pressure processor, but an autoclave is suitable, and is 105 ° C. (19.5 kPaG) or more and 135 ° C. (211.8 kPaG) or less, preferably 110 ° C. (42.0 kPaG) or more and 130 ° C. (168.9 kPaG). ), More preferably, the cell wall is subjected to a pressurized moist heat treatment for 10 to 600 minutes, preferably 30 to 180 minutes in a saturated steam atmosphere of 115 ° C. (67.8 kPaG) or more and 125 ° C. (130.8 kPaG) or less. As a result, the hydrolysis efficiency by saturated steam occurs, and the content ratio of the antioxidant component in the extract becomes high. The amount of extract extract at the time of solvent extraction increases as the treatment temperature of the pressurized moist heat treatment is higher and the treatment time is longer. However, even if the pressurized moist heat treatment exceeds 135 ° C. or exceeds 600 minutes, In the case of the solvent extraction, the amount of increase is small compared with the amount of solvent extraction in the case of performing the pressure-humidification heat treatment for 600 hours, and the pressure-humidification heat treatment temperature is less than 105 ° C or the treatment time is less than 10 minutes. A sufficient amount of extraction cannot be ensured.

(Extraction process of herbal extract with solvent)
In the present invention, as the extraction solvent used in the solvent extraction step performed after the pressurized moist heat treatment step, any known extraction solvent that is usually used for solvent extraction from plant materials can be used. As the extraction solvent, if a relatively polar solvent is used, an extract with a high concentration can be obtained. Examples of such solvents include water, lower monohydric alcohols (methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, etc.), polyhydric alcohols (glycerin, propylene glycol, butylene glycol, etc.), lower Examples include relatively high-polarity organic solvents such as alkyl esters (methyl acetate, ethyl acetate, etc.), ketones (acetone, methyl ethyl ketone, etc.) and ethers (diethyl ether, tetrahydrofuran, dipropyl ether, etc.). Or a 2 or more types of mixed solvent can be used. Particularly suitable are methanol, ethanol, 1-propanol, 2-propanol, butylene glycol, or aqueous solutions thereof.

  Extraction with a solvent can be performed according to a known method. For example, when water, a water-soluble organic solvent or a mixed solvent thereof is used as the extraction solvent, the extraction solvent amount is 1 to 50 times, preferably 5 to 20 times by weight with respect to the extraction raw material. Extraction can be performed for 0.1 to 50 hours, preferably 5 to 24 hours under a temperature condition lower than the boiling point. The extraction process may be performed in a stationary state, but it is desirable to stir moderately for more efficient extraction. Note that the extraction residue may be subjected to a re-extraction process by repeating only the pressurized moist heat treatment step and the solvent extraction step or the solvent extraction step, and this extraction operation may be repeated several times. The crude drug extract of the present invention may be blended in the skin external preparation as it is, or may be blended after removing the solvent and concentrating or drying by a known appropriate method such as vacuum concentration. Moreover, you may use what re-extracted what was extracted with the other organic solvent etc. Furthermore, a fraction obtained by fractionating and purifying the extract by a known fractionation means or purification means can also be used.

  Compared to the extract obtained by solvent extraction alone, the extract extracted by the two-step treatment of the pressurized moist heat treatment process and the solvent extraction process not only increases the extraction amount due to cell wall destruction of the ougon, but also during the pressurized moist heat treatment. The proportion of baicalin in the extract increases due to the hydrolysis of. This extract is excellent in antioxidant property, ultraviolet ray damage-inhibiting property of fibroblasts, and anti-inflammatory property, and can be used effectively as a raw material for photoaging inhibitors.

  As a photoaging inhibitor raw material, an extract of urgonum obtained by a two-stage process of a pressure-humidification heat treatment process and a solvent extraction process is effective. The argon extract extract is a caika, kakon, gymnema sylvesta, gentian shoko, shazensou, betel rouge. The combined use with at least one extract selected from the group consisting of can further enhance the photoaging inhibition effect. Here, each extract from Kaika, Kakon, Gymnema sylvestre, Gennosho, Shazenso, and betel lodge used in combination with Ogon extract is an extract obtained by a two-stage process of a pressure-humidification heat treatment process and a solvent extraction process. Even in the conventional known extraction method that does not take a pressurized moist heat treatment step, for example, an extract obtained by solvent extraction, it exhibits an excellent synergistic effect on the photoaging inhibition action, but the pressurized moist heat treatment step In addition, the extract obtained by the two-stage treatment of the solvent extraction process can obtain a more effective photoaging inhibition action. In addition, since the pressure-humidity heat treatment with saturated steam makes the crude drug extract substantially sterile, the extract extracted by this method can be used particularly suitably for cosmetics and pharmaceutical raw materials.

  Here, “Caika” means a dried legume Enju-maki. Rutin contained in silkworms is known to strengthen capillaries and reinforce blood vessels. Cuckoo is a good thing that takes the root of legume kudu and molds it, and has sweating and antipyretic action. Gymnema sylvestre is a plant belonging to the genus Musca, native to India, and its effective ingredient, Gymnema acid, is known to have an effect of suppressing obesity and diabetes. Genus is a plant belonging to the family Frostaceae, which has dried the whole grass except its roots and acts as an anti-intestinal antipruritic agent. Shazenso is a dried plant of the plantain family Psyllium, which is a diuretic, hemostatic, antitussive. As for the action, the betel waxy is a dried one of the mature seeds of coconut family betel wax.

  The herbal medicine extract of the present invention can be blended in various pharmaceuticals, quasi-drugs, cosmetics, etc. as a photoaging inhibitor component to obtain a skin external preparation. The dosage form of the external preparation for skin is not particularly limited, and can be any dosage form such as ointment, lotion, emulsion, cream, pack, granule, cataplasm, and spray. As cosmetics, skin cosmetics intended to improve the skin by photoaging are preferable. Basic cosmetics such as lotion, milky lotion, beauty essence, moisturizing cream, sunscreen cream, sunscreen lotion, suntan oil, carmine lotion, etc. Sun care products, foundations, eyeliner, mascara, eye color, teak color, makeup cosmetics such as lipstick, face wash, body shampoo, shampoo, rinse, treatment, hair cream, hair oil, hair conditioner, etc. Although it can be used for various uses such as hair cosmetics, bathing agents, deodorant antiperspirants and the like, it is of course not limited thereto.

  The blending amount of the extract of the herbal medicine according to the present invention into the external preparation for skin varies depending on the purpose and target, but is 0.0001 to 5.0% by mass in terms of nonvolatile content, preferably 0.001 to 1.0. Mass% is good. If the amount is less than 0.0001% by mass, a sufficient effect is hardly exhibited, and even if the amount exceeds 5.0% by mass, the difference in effect is small and not economical.

  Moreover, the skin external preparation of this invention can be mix | blended with the other various components normally used in the range which does not impair the effect as needed. For example, in the case of cosmetics, oil, various vitamins, surfactants, moisturizers, thickeners, preservatives, antioxidants, UV absorbers, UV scattering agents, fragrances, dyes, pigments, sequestering agents , Whitening agents, anti-inflammatory agents, astringents, cooling agents, antihistamines, sebum inhibitors, exfoliating / dissolving agents, and the like.

EXAMPLES Hereinafter, although this invention is explained in full detail based on an Example, this invention is not limited by these. Moreover,% shown in the Examples means mass% unless otherwise specified.
Production Example 1
20 g of Ogon increments were subjected to pressurized moist heat treatment at 120 ° C. for 30 minutes in a high pressure steam sterilizer, HV-85, manufactured by Hirayama Seisakusho. After cooling, solvent extraction was performed with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain Ougon extract 1.

Production Example 2
Solvent extraction was performed on 20 g of Augon tick with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain Ougon extract 2.

Production Example 3
30 ml of water was added to 20 g of Augon, and the mixture was heated at 60 ° C. for 6 hours. Then, 30 ml of ethanol was further added, and solvent extraction was performed at 60 ° C. for 12 hours. After cooling, it was filtered to obtain Ougon extract 3.

Production Example 4
20 g of Kaika was subjected to pressurized moist heat treatment at 120 ° C. for 30 minutes using a high pressure steam sterilizer, HV-85, manufactured by Hirayama Seisakusho. After cooling, solvent extraction was performed with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain a silkworm extract 1.

Production Example 5
20 g of Kaika was subjected to solvent extraction with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain a silkworm extract 2.

Production Example 6
30 ml of water was added to 20 g of Kaika and heated at 60 ° C. for 6 hours, and then 30 ml of ethanol was further added, followed by solvent extraction at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain a silkworm extract 3.

Production Example 7
20 g of the konkon was subjected to pressurized heat treatment at 120 ° C. for 30 minutes using a high pressure steam sterilizer, HV-85, manufactured by Hirayama Seisakusho. After cooling, solvent extraction was performed with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain Cuckon extract 1.

Production Example 8
Solvent extraction was performed on 20 g of kakonkon in 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was carried out to obtain Cuckon extract 2.

Production Example 9
30 ml of water was added to 20 g of cocoon and heated at 60 ° C. for 6 hours, and then 30 ml of ethanol was further added, followed by solvent extraction at 60 ° C. for 12 hours. After cooling, it was filtered to obtain Cuckon extract 3.

Production Example 10
20g of Gymnema Sylvester was subjected to pressurized moist heat treatment at 120 ° C for 30 minutes using a high pressure steam sterilizer, HV-85, manufactured by Hirayama Seisakusho. After cooling, solvent extraction was performed with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain Gymnema sylvestre extract 1.

Production Example 11
20 g of Gymnema sylvesta was extracted with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain Gymnema sylvestre extract 2.

Production Example 12
30 ml of water was added to 20 g of Gymnema sylvestre and heated at 60 ° C. for 6 hours, and then 30 ml of ethanol was further added, followed by solvent extraction at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain Gymnema sylvestre extract 3.

Production Example 13
20 g of gennosho chopping was subjected to pressure and humidity heat treatment at 120 ° C. for 30 minutes using a high pressure steam sterilizer, HV-85, manufactured by Hirayama Seisakusho. After cooling, solvent extraction was performed with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, it was filtered to obtain Genokosho extract 1.

Production Example 14
Solvent extraction was performed on 20 g of Gennosho chops with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain Genokosho extract 2.

Production Example 15
30 ml of water was added to 20 g of genkosho and heated at 60 ° C. for 6 hours, and then 30 ml of ethanol was further added, followed by solvent extraction at 60 ° C. for 12 hours. After cooling, it was filtered to obtain Genokosho extract 3.

Production Example 16
A 20 g portion of shazenso was subjected to a pressure and heat treatment at 120 ° C. for 30 minutes in a high-pressure steam sterilizer, HV-85, manufactured by Hirayama Seisakusho. After cooling, solvent extraction was performed with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was carried out to obtain Chasenso extract 1.

Production Example 17
Solvent extraction was performed on 20 g of shazenso increments with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was carried out to obtain Shazenso extract 2.

Production Example 18
30 ml of water was added to 20 g of shazenso and heated at 60 ° C. for 6 hours, 30 ml of ethanol was further added, and solvent extraction was performed at 60 ° C. for 12 hours. After cooling, filtration was carried out to obtain Shazenso extract 3.

Production Example 19
20 g of betel lump was subjected to pressurized moist heat treatment at 120 ° C. for 30 minutes in a high pressure steam sterilizer, HV-85, manufactured by Hirayama Seisakusho. After cooling, solvent extraction was performed with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was carried out to make a betel wax extract 1.

Production Example 20
Solvent extraction was performed on 20 g of betel litter with 60 ml of 50% ethanol at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain a betel wax extract 2.

Production Example 21
30 ml of water was added to 20 g of betel nut and heated at 60 ° C. for 6 hours, and then 30 ml of ethanol was further added, followed by solvent extraction at 60 ° C. for 12 hours. After cooling, filtration was performed to obtain a betel wax extract 3.

Test Example 1 Measurement of Non-Volatile Content In order to compare the extraction efficiency of extracts, 5 g of each extract obtained in Production Examples 1 to 21 was collected in a glass petri dish and measured for non-volatile content after absolutely dry at 105 ° C. for 3 hours. did.

Test Example 2 Baicalein Concentration in Nonvolatile Content of Ougone Extract Extract The baicalein concentration in the nonvolatile content of ougon extract extract obtained in Test Example 1 was determined by high performance liquid chromatography.
Column: HRC-ODS (40 ° C .: manufactured by Shimadzu Corporation)
Detector: SPD-M10A (268 nm: manufactured by Shimadzu Corporation)
Mobile phase: 30% acetonitrile (containing 0.1% phosphoric acid)
Flow rate: 1 ml / min Sample: Baicalein, 98% (Aldrich)

Test Example 3 Antioxidant of Extract Extract In a test tube, 2.3 ml of 0.05 M sodium carbonate, 0.1 ml of 3 mM xanthine, 0.1 ml of 3 mM EDTA, 0.1 ml of 0.15% albumin 0.1 ml of 0.75 mM nitroblue tetrazolium and 0.1 ml of the extract of Preparation Examples 1 to 14 adjusted with 50% ethanol so as to be 0.3 mg / ml in terms of non-volatile content, were collected at 25 ° C. After shaking for 10 minutes, 0.1 ml of 0.1 u / ml xanthine oxidase was added, and the mixture was further shaken for 20 minutes to carry out the reaction. Immediately after completion, 0.1 ml of 6 mM copper chloride was added to stop the reaction, and the reduction inhibition rate was calculated from the absorbance (OD) at 570 nm of the reaction solution. For the control, the same operation was performed without adding xanthine oxidase. After the reaction was completed, copper chloride was added, and further xanthine oxidase was added. Moreover, 50% ethanol was used for the blank.

Test Example 4 Inhibition of UV damage of fibroblasts Inhibition of UV damage of fibroblasts was determined from the survival rate of MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyl tetrazolium bromide). 10 × 10 ⁴ cells of TIG-113 (PDL = 33) obtained from the Human Science Foundation were seeded on a 48-well multiplate, 250 μl of medium (including EMEM-FBS 0.5%) was added and cultured for 2 days. After exchanging the medium, the extract of Production Examples 1 to 14 adjusted with 50% ethanol so as to be 0.025% in terms of non-volatile content (the total concentration is the same in the case of complex system, blending ratio = 1: 1) 20 μl After culturing in a 37 ° C. incubator for 1 hour, UV irradiation was performed for 10 minutes with Philips UV low pressure mercury lamp PL-S 9W / 01, followed by culturing again in a 37 ° C. incubator for 24 hours. After incubation, the plate was washed once with 250 μl of PBS (−), 250 μl of MTT 0.5 mg / ml dissolved in the medium was added, and staining was performed in a 37 ° C. incubator for 3 hours. After staining, the plate was washed once with 250 μl of PBS (−), extracted with 500 μl of an isopropyl alcohol solution containing 0.4% hydrochloric acid, and the absorbance (OD) at 570 nm was measured. In addition, 50% ethanol was added to the blank, and 50% ethanol was added to the non-ultraviolet-irradiated control, and the cell viability with respect to the blank was determined from the following formula. In addition, ascorbic acid was compared as a positive control. (The number of measurements for each sample was 3, and the average value of the cell viability was determined.)

Test Example 5 Measurement of Lactate Dehydrogenase (LDH) When skin cells are damaged by ultraviolet light, intracellular Ca concentration increases, and intracellular inclusions such as lactate dehydrogenase (LDH) and prostaglandin E2 (PGE2) It is supposed to release things. It is known that when the degree of damage by ultraviolet rays is large, the cells are necrotic, and as a result, an inflammatory reaction is induced and further skin damage is caused. By measuring LDH, the intensity of inflammation can be discriminated. LDH in the liquid was measured using the culture liquid cultured for 24 hours after the ultraviolet irradiation in Test Example 4 as a sample. Test example 2.65 ml of 0.1 M phosphate buffer (pH = 7), 0.1 ml of 23 mM pyruvate, 0.05 ml of 12 mM β-nicotinamide adenine dinucleotide · 2Na (NADH) 0.2 ml of sample 4 was collected, absorbance (340 nm) at 25 ° C. was measured for 5 minutes, ΔE / min was calculated, and LDH was determined from the following formula. Here, the molar extinction coefficient ε at 340 nm of NADH was set to 6.3 (cm ² / μmol).

ここで、Ｖ：総液量（ml)
ε：ＮＡＤＨのモル吸光係数（cm²／μmol）
ｌ：セル長（cm）
ｖ：検体量（ml)
ΔＥ／ｍｉｎ：１分間あたりの吸光度変化

Where V: total liquid volume (ml)
ε: molar absorption coefficient of NADH (cm ² / μmol)
l: Cell length (cm)
v: Sample volume (ml)
ΔE / min: change in absorbance per minute

The extract extract concentration increases in the order of the extraction method using only the solvent extraction step, the pretreatment method using water at 60 ° C., and the extraction method based on the two-stage treatment of the pressurized moist heat treatment step and the solvent extraction step. For Test Example 3 to Test Example 5, the effect of the extract extracted only in the solvent extraction process with the lowest concentration and the effect of the extract extracted in the two-stage process of the pressurized wet heat treatment process and the solvent extraction process with the highest concentration were compared. However, in the antioxidant of Test Example 3, for all of the herbal medicines tested this time, the extract extracted by the two-stage treatment of the pressurized moist heat treatment process and the solvent extraction process is higher than the extract extracted by the solvent extraction process alone. Is shown. Since the amount used is adjusted in terms of non-volatile content, it is considered that not only Ogon but also other herbal medicines, the products converted by the pressure and moist heat treatment process contribute to this effect. Ougon extract 1 extracted by the two-stage treatment of the pressure-humidity heat treatment process and the solvent extraction process of the present invention shows good values for both cell viability and LDH, compared to the ougon extract 2 obtained only by the solvent extraction process. . By using the extract of Ougon according to the present invention in combination with the extract of Kaika, Kakon, Gymnema sylvestre, Gennoshoco, Shazenso, and betel palm, a remarkable synergistic effect is observed in the cell viability and LDH values, and only by the solvent extraction process. The combined use of the extract by the two-stage treatment in the pressure and humidity heat treatment process shows a better effect than the extract.
Below, the formulation example of this invention is shown.

Formulation Example (A) Hydroxyethylcellulose (2% aqueous solution) 12.0% by mass
Xanthan gum (2% aqueous solution) 2.0% by mass
Purified water Residual (B) Lecithin hydroxide 0.3% by mass
1,3-butylene glycol 6.0% by mass
Glycerin 4.0% by mass
Polyethylene glycol 2.0
Methyl paraben Appropriate amount (C) Extract of the present invention (1% solution) 3.0% by mass
Sodium hyaluronate (1% aqueous solution) 5.0% by mass
Citric acid (5% aqueous solution) 2.8% by mass
Sodium citrate (5% aqueous solution) 2.2% by mass

  The component (B) is heated to 50 ° C. to dissolve methyl paraben, and then returned to room temperature. Other components are made uniform by stirring at room temperature. While stirring the component (A), the components (B) and (C) are added in this order and mixed uniformly.

  By using the external photo-aging inhibitor according to the present invention, which contains an extract extracted from ougon with a strong photo-aging inhibitory effect, obtained in a high yield by a two-stage treatment of a pressurized moist heat treatment step and a solvent extraction step, photo-aging of the skin is achieved. Can be expected to maintain a firm, elastic, youthful skin condition.

Claims (8)

  A dry product of herbal medicine selected from Ogon, Kaika, Kakon, Gymnema sylvestre, Gennosho, Shazensou and Beinrouji is saturated steam at 105 ° C. (19.5 kPaG) or more and 135 ° C. (211.8 kPaG) or less in a pressurized humid heat treatment apparatus An extract of herbal medicine obtained by performing a two-step process of solvent extraction using a polar solvent, followed by pressurizing and moist heat treatment in an atmosphere.   As polar solvents, water, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol lower monohydric alcohol, glycerin, propylene glycol, butylene glycol polyhydric alcohol, methyl acetate, ethyl acetate The extract of a herbal medicine according to claim 1, obtained by using a certain lower alkyl ester, acetone, a ketone that is methyl ethyl ketone, diethyl ether, tetrahydrofuran, an ether that is dipropyl ether, or any mixture of these polar solvents.   A dry product of herbal medicine selected from Ogon, Kaika, Kakon, Gymnema sylvestre, Gennosho, Shazensou and Beinrouji is saturated steam at 105 ° C. (19.5 kPaG) or more and 135 ° C. (211.8 kPaG) or less in a pressurized humid heat treatment apparatus A method for producing an extract of a herbal medicine, which is extracted by a two-step process of solvent extraction using a polar solvent, followed by pressure and humidity heat treatment in an atmosphere.   As polar solvents, water, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol lower monohydric alcohol, glycerin, propylene glycol, butylene glycol polyhydric alcohol, methyl acetate, ethyl acetate 4. The method for producing an extract of a herbal medicine according to claim 3, wherein a certain lower alkyl ester, acetone, methyl ethyl ketone, ketone, diethyl ether, tetrahydrofuran, dipropyl ether, or any mixture of these polar solvents is used.   Two stages of solvent extraction using a polar solvent with a wet steam heat treatment in a saturated steam atmosphere of 105 ° C (19.5 kPaG) to 135 ° C (211.8 kPaG) An anti-photoaging skin external preparation containing, as an active ingredient, an extract of urgon, extracted by treatment.   Ougon extract is water, methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol as a polar solvent, lower monohydric alcohol, glycerin, propylene glycol, butylene glycol, polyhydric alcohol, acetic acid 6. It is obtained using methyl, lower alkyl ester which is ethyl acetate, acetone, ketone which is methyl ethyl ketone, diethyl ether, tetrahydrofuran, ether which is dipropyl ether or any mixture of these polar solvents. The photoaging prevention skin external preparation as described.   A photoaging-inhibiting skin external preparation containing at least one extract selected from the group consisting of Kaika, Kakon, Gymnema sylvestre, Gennoshoco, Shazenso, and betel palm, in addition to the Ogon extract of claim 5.   8. The photoaging-inhibiting skin external preparation according to claim 7, wherein the extract of Kaika, Kakon, Gymnema sylvestre, Gennosho, Shazenso, and betel rosy is the extract of the herbal medicine according to claim 1.