JPH0787785B2 - 改良ベクタおよびこの種ベクタの製造方法ならびにクローン化遺伝子の発現方法 - Google Patents

改良ベクタおよびこの種ベクタの製造方法ならびにクローン化遺伝子の発現方法

Info

Publication number
JPH0787785B2
JPH0787785B2 JP56047362A JP4736281A JPH0787785B2 JP H0787785 B2 JPH0787785 B2 JP H0787785B2 JP 56047362 A JP56047362 A JP 56047362A JP 4736281 A JP4736281 A JP 4736281A JP H0787785 B2 JPH0787785 B2 JP H0787785B2
Authority
JP
Japan
Prior art keywords
gene
site
fragment
dna
bam
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP56047362A
Other languages
English (en)
Japanese (ja)
Other versions
JPS5714599A (en
Inventor
ワルテル・シヤルル・フイ−ルス
エリク・ルネ・ルモ−
Original Assignee
バイオゲン インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27260930&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JPH0787785(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by バイオゲン インコーポレイテッド filed Critical バイオゲン インコーポレイテッド
Publication of JPS5714599A publication Critical patent/JPS5714599A/ja
Publication of JPH0787785B2 publication Critical patent/JPH0787785B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/22011—Polyomaviridae, e.g. polyoma, SV40, JC
    • C12N2710/22022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
JP56047362A 1980-06-06 1981-04-01 改良ベクタおよびこの種ベクタの製造方法ならびにクローン化遺伝子の発現方法 Expired - Lifetime JPH0787785B2 (ja)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB18701 1980-06-06
GB8018701 1980-06-06
GB8026661 1980-08-15
GB28983 1980-09-08
GB8028983 1980-09-08
GB26661 1980-09-08

Related Child Applications (3)

Application Number Title Priority Date Filing Date
JP10784695A Division JP3158951B2 (ja) 1980-06-06 1995-04-10 改良組換ｄｎａ分子
JP10784795A Division JP3158952B2 (ja) 1980-06-06 1995-04-10 組換ｄｎａ分子の作製方法
JP10784895A Division JP3158953B2 (ja) 1980-06-06 1995-04-10 改良Ｅ．ｃｏｌｉ宿主細胞

Publications (2)

Publication Number Publication Date
JPS5714599A JPS5714599A (en) 1982-01-25
JPH0787785B2 true JPH0787785B2 (ja) 1995-09-27

Family

ID=27260930

Family Applications (6)

Application Number Title Priority Date Filing Date
JP56047362A Expired - Lifetime JPH0787785B2 (ja) 1980-06-06 1981-04-01 改良ベクタおよびこの種ベクタの製造方法ならびにクローン化遺伝子の発現方法
JP10784795A Expired - Lifetime JP3158952B2 (ja) 1980-06-06 1995-04-10 組換ｄｎａ分子の作製方法
JP10784695A Expired - Lifetime JP3158951B2 (ja) 1980-06-06 1995-04-10 改良組換ｄｎａ分子
JP10784895A Expired - Lifetime JP3158953B2 (ja) 1980-06-06 1995-04-10 改良Ｅ．ｃｏｌｉ宿主細胞
JP10060225A Pending JPH10304876A (ja) 1980-06-06 1998-03-11 改良組換ｄｎａ分子
JP2000314383A Pending JP2001136966A (ja) 1980-06-06 2000-10-13 改良組換ｄｎａ分子

Family Applications After (5)

Application Number Title Priority Date Filing Date
JP10784795A Expired - Lifetime JP3158952B2 (ja) 1980-06-06 1995-04-10 組換ｄｎａ分子の作製方法
JP10784695A Expired - Lifetime JP3158951B2 (ja) 1980-06-06 1995-04-10 改良組換ｄｎａ分子
JP10784895A Expired - Lifetime JP3158953B2 (ja) 1980-06-06 1995-04-10 改良Ｅ．ｃｏｌｉ宿主細胞
JP10060225A Pending JPH10304876A (ja) 1980-06-06 1998-03-11 改良組換ｄｎａ分子
JP2000314383A Pending JP2001136966A (ja) 1980-06-06 2000-10-13 改良組換ｄｎａ分子

Country Status (17)

Country Link
EP (1) EP0041767B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (6) JPH0787785B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
KR (1) KR860001557B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
BG (1) BG60443B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
BR (1) BR8101972A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA1207251A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DD (1) DD158918A5 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DE (1) DE3177298T2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DK (1) DK171301B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ES (1) ES500967A0 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
FI (1) FI811009L (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
GR (1) GR74282B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IE (1) IE63126B1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IL (1) IL62553A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
NO (1) NO811119L (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
PH (1) PH22618A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
YU (1) YU87581A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL58765A (en) * 1979-11-21 1986-09-30 Yeda Res & Dev Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta
US5554513A (en) * 1979-11-21 1996-09-10 Yeda Research & Development Co. Ltd. Production of recombinant human interferon-beta2
US5510472A (en) * 1979-11-21 1996-04-23 Yeda Research And Development Co. Ltd. Production of recombinant human interferon-beta2
AU564690B2 (en) 1980-04-03 1987-08-20 Biogen Idec Ma Inc. Process for producing human fibroblast interferon-like polypeptides using recombinant dna
IL65475A0 (en) * 1981-04-17 1982-07-30 Meloy Lab Production of immune interferon and its mrna
EP0068693A3 (en) * 1981-06-16 1983-07-20 Genentech, Inc. Production of foot and mouth disease vaccine from microbially expressed antigens
JPS5835198A (ja) * 1981-08-28 1983-03-01 Mitsubishi Petrochem Co Ltd 新規ベクター
ZA831854B (en) * 1982-03-26 1984-01-25 Biogen Nv Small peptides with the specificity of foot and mouth disease viral antigens
JPS58183659A (ja) * 1982-03-31 1983-10-26 ジェネテイックス、インスチチュ−ト、インコ−ポレ−テッド 修飾プロインシュリン前駆体、これを暗号化するｄｎａ配列およびそれらの産生方法
FR2526661B1 (fr) * 1982-05-13 1986-02-21 Transgene Sa Nouveaux vecteurs d'expression de la proteine antigenique de la rage et leur application a la preparation de vaccins
US4582800A (en) * 1982-07-12 1986-04-15 Hoffmann-La Roche Inc. Novel vectors and method for controlling interferon expression
CA1209501A (en) * 1982-09-16 1986-08-12 Nikos Panayotatos Expression vector
CA1341012C (en) * 1982-09-27 2000-06-06 Biogen, Inc. Dna sequences, recombinant dna molecules and processes for producing swine growth hormone-like polypeptides
AU575301B2 (en) * 1982-10-08 1988-07-28 Juridical Foundation, Japanese Foundation For Cancer Research Novel vector
FR2539758B1 (fr) * 1983-01-21 1985-11-15 Transgene Sa Nouveaux vecteurs d'expression et leur application a la preparation d'une proteine ayant l'activite de l'antitrypsine-a1 humaine
WO1984002918A1 (fr) * 1983-01-21 1984-08-02 Transgene Sa NOUVEAUX VECTEURS D'EXPRESSION ET LEUR APPLICATION A LA PREPARATION D'UNE PROTEINE AYANT L'ACTIVITE DE L'ANTITRYPSINE-alpha1 HUMAINE
GB8308235D0 (en) * 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
DE3319242A1 (de) * 1983-05-27 1984-11-29 Battelle-Institut E.V., 6000 Frankfurt Verfahren und plasmid zur steuerung der expression von klonierten genen
US5670371A (en) * 1983-07-15 1997-09-23 Bio-Technology General Corp. Bacterial expression of superoxide dismutase
BG49718A3 (bg) * 1983-07-15 1992-01-15 Bio- Technology General Corp Метод за получаване на полипептид със супероксиддисмутазна активност
US5162216A (en) * 1983-09-23 1992-11-10 Enzon Labs Inc. Hybrid gene regulatory region operable in E. coli
FR2556365B1 (fr) * 1983-12-09 1987-07-31 Transgene Sa Vecteurs de clonage et d'expression de l'interferon-g, bacteries transformees et procede de preparation de l'interferon-g
ATE100494T1 (de) * 1984-02-08 1994-02-15 Cetus Oncology Corp Kontrollsysteme fuer rekombinant-manipulationen.
US4711845A (en) * 1984-08-31 1987-12-08 Cetus Corporation Portable temperature-sensitive control cassette
US5081020A (en) * 1984-08-27 1992-01-14 Bio-Technology General Corp. Expression vectors containing λPL promoter, T1 T2 rRNA termination sequence and origin of replication derived from a constitutive high copy number plasmid hosts and related methods
US5059529A (en) * 1984-08-27 1991-10-22 Bio-Technology General Corp. Stabilized expression vectors containing λPL promoter and the gene for the cI434 repressor, plasmids containing the vectors, hosts containing the plasmids and related methods
US5143836A (en) * 1984-08-27 1992-09-01 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda pl promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
CA1340597C (en) * 1984-08-27 1999-06-22 Haim Aviv Expression vectors containing pl promoter, and engineered restriction site for convenient replacement of ribosomal binding site, plasmids containing the vectors, hosts containing the plasmids and related methods
US5126252A (en) * 1984-08-27 1992-06-30 Bio-Technology General Corp. Expressions vectors containing Lambda PL promoter and T1 T2 RNA termination sequence, plasmids containing the vectors, hosts containing the plasmids and relatedmethods
US5759816A (en) * 1984-08-27 1998-06-02 Bio-Technology General Corp. Expression vectors containing λPL promoter and T1 T2 rRNA termination sequence plasmids containing the vectors hosts containing the plasmids and related methods
US5162217A (en) * 1984-08-27 1992-11-10 Bio-Technology General Corp. Plasmids for expression of human superoxide dismutase (SOD) analogs containing lambda PL promoter with engineered restriction site for substituting ribosomal binding sites and methods of use thereof
US5112744A (en) * 1984-08-27 1992-05-12 Bio-Technology General Corp. Plasmids for the production of human Cu-Zn superoxide dismutase, bacterial hosts containing the plasmids and methods for production and recovery of such superoxide dismutase
US4766066A (en) * 1984-09-27 1988-08-23 Eli Lilly And Company Method of using bacteriophage lambda p1 promoter to produce a functional polypeptide in streptomyces
US5510238A (en) * 1984-10-26 1996-04-23 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Production of human T-cell leukemia (lymphotropic) retrovirus (HTLV-I) envelope protein fragments in bacteria and use in seroepidemiological studies
WO1986003751A2 (en) 1984-12-21 1986-07-03 Biogen N.V. Purification, production and use of tumor necrosis factors
US5182196A (en) * 1985-10-09 1993-01-26 Biogen, Inc. Expression systems for overproduction of desired proteins
ATE123526T1 (de) * 1987-08-17 1995-06-15 Hoffmann La Roche Hochreprimierbare expressionskontrollsequenzen.
JP2003204783A (ja) * 1999-07-19 2003-07-22 Ajinomoto Co Inc 発酵法による目的物質の製造法
US7579005B2 (en) 2005-11-28 2009-08-25 E. I. Du Pont De Nemours And Company Process for recombinant expression and purification of antimicrobial peptides using periplasmic targeting signals as precipitable hydrophobic tags

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU564690B2 (en) * 1980-04-03 1987-08-20 Biogen Idec Ma Inc. Process for producing human fibroblast interferon-like polypeptides using recombinant dna
IN156358B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * 1980-05-12 1985-07-06 Biogen Nv

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
ＭｅｔｈｏｄｓｉｎＥｎｚｙｍｏｌｏｇｙＶｏｌ．６８Ｐ．４８２−４９２

Also Published As

Publication number Publication date
DE3177298D1 (de) 1993-04-08
JP3158952B2 (ja) 2001-04-23
KR860001557B1 (ko) 1986-10-04
IL62553A0 (en) 1981-06-29
DD158918A5 (de) 1983-02-09
DK148681A (da) 1981-12-07
ES8302774A1 (es) 1983-01-16
JPH0838177A (ja) 1996-02-13
IL62553A (en) 1984-12-31
FI811009A7 (fi) 1981-12-07
JP2001136966A (ja) 2001-05-22
NO811119L (no) 1981-12-07
JP3158951B2 (ja) 2001-04-23
JPH0838179A (ja) 1996-02-13
IE63126B1 (en) 1995-03-22
IE810757L (en) 1981-12-06
JPH10304876A (ja) 1998-11-17
EP0041767B1 (en) 1993-03-03
BG60443B2 (bg) 1995-03-31
YU87581A (en) 1984-04-30
BR8101972A (pt) 1982-08-17
DE3177298T2 (de) 1993-07-01
JPH0838176A (ja) 1996-02-13
EP0041767A3 (en) 1982-09-29
JPS5714599A (en) 1982-01-25
GR74282B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1984-06-21
DK171301B1 (da) 1996-08-26
KR830005353A (ko) 1983-08-13
ES500967A0 (es) 1983-01-16
CA1207251A (en) 1986-07-08
JP3158953B2 (ja) 2001-04-23
EP0041767A2 (en) 1981-12-16
PH22618A (en) 1988-10-28
FI811009L (fi) 1981-12-07

Similar Documents

Publication Publication Date Title
JPH0787785B2 (ja) 改良ベクタおよびこの種ベクタの製造方法ならびにクローン化遺伝子の発現方法
US4758512A (en) Hosts and methods for producing recombinant products in high yields
US4349629A (en) Plasmid vectors, production anduse thereof
JP2530801B2 (ja) 組換えｄｎａ分子
US5401642A (en) Vectors and methods for making such vectors and for expressing cloned genes
JPH0724582B2 (ja) トリプトファン・プロモーター―オペレーターを用いる細菌によるポリペプチドの発現用ベクター
US5232840A (en) Enhanced protein production in bacteria by employing a novel ribosome binding site
US4716112A (en) Vectors for increased expression of cloned genes
JP2544710B2 (ja) β−ラクタマ―ゼの製法
EP0182442B1 (en) Recombinant dna molecules and their method of production
IE67890B1 (en) Enhanced Protein production in bacteria by employing a novel ribosome binding site
US4853333A (en) Production of rotavirus in yeast
JP2574142B2 (ja) 組換えリシン毒素フラグメント
JP2515488B2 (ja) ポリペプチドの製造方法
US4845031A (en) Recombinant DNA process for producing useful polypeptides
CA1275954C (en) 3'-expression enhancing fragments and method
EP0133044A1 (en) Stabilising foreign proteins, and plasmids for use therein
SI7913127A8 (sl) Rekombinantne dna molekule in postopek za njihovo proizvodnjo
HU196236B (en) Process for producing polypeptides showing hepatitis b virus antigenicity