JPH0781987B2 - 等電点電気泳動法及びそのための装置 - Google Patents

等電点電気泳動法及びそのための装置

Info

Publication number
JPH0781987B2
JPH0781987B2 JP63085487A JP8548788A JPH0781987B2 JP H0781987 B2 JPH0781987 B2 JP H0781987B2 JP 63085487 A JP63085487 A JP 63085487A JP 8548788 A JP8548788 A JP 8548788A JP H0781987 B2 JPH0781987 B2 JP H0781987B2
Authority
JP
Japan
Prior art keywords
chamber
gradient
flow
compound
isoelectric point
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP63085487A
Other languages
English (en)
Japanese (ja)
Other versions
JPS63263457A (ja
Inventor
ミッシェル フォペル ダニエル
ジオルジオ リゲッティ ピエール
Original Assignee
チバ‐ガイギー アクチェンゲゼルシャフト
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878708746A external-priority patent/GB8708746D0/en
Application filed by チバ‐ガイギー アクチェンゲゼルシャフト filed Critical チバ‐ガイギー アクチェンゲゼルシャフト
Publication of JPS63263457A publication Critical patent/JPS63263457A/ja
Publication of JPH0781987B2 publication Critical patent/JPH0781987B2/ja
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
JP63085487A 1987-04-11 1988-04-08 等電点電気泳動法及びそのための装置 Expired - Lifetime JPH0781987B2 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB878708746A GB8708746D0 (en) 1987-04-11 1987-04-11 Electrophoretic process
GB8708746 1987-04-11
GB878728289A GB8728289D0 (en) 1987-04-11 1987-12-03 Isoelectric focusing process & means for carrying out said process
GB8728289 1987-12-03

Publications (2)

Publication Number Publication Date
JPS63263457A JPS63263457A (ja) 1988-10-31
JPH0781987B2 true JPH0781987B2 (ja) 1995-09-06

Family

ID=26292133

Family Applications (1)

Application Number Title Priority Date Filing Date
JP63085487A Expired - Lifetime JPH0781987B2 (ja) 1987-04-11 1988-04-08 等電点電気泳動法及びそのための装置

Country Status (12)

Country Link
US (2) US4971670A (enExample)
EP (1) EP0287513B1 (enExample)
JP (1) JPH0781987B2 (enExample)
CA (1) CA1335805C (enExample)
DE (1) DE3876273T2 (enExample)
DK (1) DK174962B1 (enExample)
ES (1) ES2037273T3 (enExample)
FI (1) FI97893C (enExample)
GR (1) GR3007124T3 (enExample)
HK (1) HK192495A (enExample)
IE (1) IE62803B1 (enExample)
PT (1) PT87210B (enExample)

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US7857957B2 (en) * 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
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AUPP971399A0 (en) 1999-04-12 1999-05-06 Life Therapeutics Limited Separation of plasma components
US6284115B1 (en) 1999-09-21 2001-09-04 Agilent Technologies, Inc. In-line flow through micro-dialysis apparatus and method for high performance liquid phase separations
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WO2001036071A1 (en) * 1999-11-16 2001-05-25 Champagne James T Solution based two-dimensional separation and detection of amphoteric substances
US7077942B1 (en) * 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
US20080096284A1 (en) * 2000-02-08 2008-04-24 Regents Of The University Of Michigan Protein separation and analysis
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US6638408B1 (en) 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
AUPQ691400A0 (en) 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
AUPQ697300A0 (en) 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
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EP1561756B1 (en) * 2002-09-11 2015-12-23 Chugai Seiyaku Kabushiki Kaisha Method of purifying protein
DE60324458D1 (de) * 2002-11-28 2008-12-11 Arkray Inc Verfahren und apparat zum anreichern und zur aufreinigung von nukleinsäure
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
CA2537636A1 (en) * 2003-09-03 2005-03-10 Shmuel Bukshpan Methods and apparatus for rapid crystallization of biomolecules
WO2005036153A1 (en) * 2003-10-07 2005-04-21 Invitrogen Corporation Improved isoelectric focusing gels and methods of use thereof
CN1878863A (zh) * 2003-11-10 2006-12-13 爱科来株式会社 核酸的浓缩精制方法及装置
US20050197496A1 (en) * 2004-03-04 2005-09-08 Gtc Biotherapeutics, Inc. Methods of protein fractionation using high performance tangential flow filtration
US7833625B2 (en) * 2004-03-17 2010-11-16 Bio-Rad Laboratories, Inc. Materials, methods and systems for separating and identifying proteins from mixtures
WO2005089910A1 (en) * 2004-03-17 2005-09-29 Ciphergen Biosystems, Inc. Multi-compartment filter and method of filtering using same
US20050249667A1 (en) * 2004-03-24 2005-11-10 Tuszynski Jack A Process for treating a biological organism
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
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US20060130159A1 (en) * 2004-12-09 2006-06-15 Nick Masiello Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum
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US7531632B2 (en) * 2006-02-16 2009-05-12 Gtc Biotherapeutics, Inc. Clarification of transgenic milk using depth filtration
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US20120043210A9 (en) * 2007-10-09 2012-02-23 Doucette Alan A Apparatus for Purifying Molecules
US8282803B2 (en) 2009-03-25 2012-10-09 Bio-Rad Laboratories, Inc. Isoelectric focusing tray and electrode assembly for alternate gel strip orientations
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US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
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US9182372B2 (en) 2011-11-22 2015-11-10 Stephen G. Haralampu Stopped-flow, micro-fluidic device and method for the charge-based separation of complex analyte mixtures
CN104254390B (zh) 2012-02-15 2017-05-10 生物辐射实验室股份有限公司 pH和离子强度的电子控制
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US9671368B2 (en) * 2013-05-10 2017-06-06 The Regents Of The University Of California Two-dimensional microfluidic devices and methods of using the same
CA2916566A1 (fr) 2013-07-05 2015-01-08 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Matrice de chromatographie d'affinite
JP6462681B2 (ja) 2013-07-12 2019-01-30 ジェネンテック， インコーポレイテッド イオン交換クロマトグラフィーのインプットの最適化の解明

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3844925A (en) 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation

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SE415731B (sv) * 1977-04-26 1980-10-27 Pharmacia Fine Chemicals Ab Vattenloslig amfolyt for separationsendamal samt sett att framstella densamma
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Patent Citations (1)

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US3844925A (en) 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation

Also Published As

Publication number Publication date
EP0287513A2 (en) 1988-10-19
PT87210A (pt) 1989-05-12
DK174962B1 (da) 2004-03-29
DK191388A (da) 1988-10-12
US4971670A (en) 1990-11-20
FI97893B (fi) 1996-11-29
HK192495A (en) 1995-12-29
FI97893C (fi) 1997-03-10
IE881067L (en) 1988-10-11
EP0287513A3 (en) 1990-10-10
DK191388D0 (da) 1988-04-08
US5082548A (en) 1992-01-21
FI881652A7 (fi) 1988-10-12
PT87210B (pt) 1993-11-30
ES2037273T3 (es) 1993-06-16
FI881652A0 (fi) 1988-04-08
GR3007124T3 (enExample) 1993-07-30
DE3876273D1 (de) 1993-01-14
DE3876273T2 (de) 1993-05-27
IE62803B1 (en) 1995-03-08
EP0287513B1 (en) 1992-12-02
JPS63263457A (ja) 1988-10-31
CA1335805C (en) 1995-06-06

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