DK174962B1 - Fremgangsmåde til isoelektrisk fokusering samt apparat til udøvelse af fremgangsmåden - Google Patents

Fremgangsmåde til isoelektrisk fokusering samt apparat til udøvelse af fremgangsmåden Download PDF

Info

Publication number
DK174962B1
DK174962B1 DK198801913A DK191388A DK174962B1 DK 174962 B1 DK174962 B1 DK 174962B1 DK 198801913 A DK198801913 A DK 198801913A DK 191388 A DK191388 A DK 191388A DK 174962 B1 DK174962 B1 DK 174962B1
Authority
DK
Denmark
Prior art keywords
isoelectric
amphoteric
immobilized
membranes
isoelectric point
Prior art date
Application number
DK198801913A
Other languages
Danish (da)
English (en)
Other versions
DK191388A (da
DK191388D0 (da
Inventor
Daniel Michel Faupel
Pier Giorgio Righetti
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878708746A external-priority patent/GB8708746D0/en
Application filed by Novartis Ag filed Critical Novartis Ag
Publication of DK191388D0 publication Critical patent/DK191388D0/da
Publication of DK191388A publication Critical patent/DK191388A/da
Application granted granted Critical
Publication of DK174962B1 publication Critical patent/DK174962B1/da

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
DK198801913A 1987-04-11 1988-04-08 Fremgangsmåde til isoelektrisk fokusering samt apparat til udøvelse af fremgangsmåden DK174962B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB8708746 1987-04-11
GB878708746A GB8708746D0 (en) 1987-04-11 1987-04-11 Electrophoretic process
GB878728289A GB8728289D0 (en) 1987-04-11 1987-12-03 Isoelectric focusing process & means for carrying out said process
GB8728289 1987-12-03

Publications (3)

Publication Number Publication Date
DK191388D0 DK191388D0 (da) 1988-04-08
DK191388A DK191388A (da) 1988-10-12
DK174962B1 true DK174962B1 (da) 2004-03-29

Family

ID=26292133

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198801913A DK174962B1 (da) 1987-04-11 1988-04-08 Fremgangsmåde til isoelektrisk fokusering samt apparat til udøvelse af fremgangsmåden

Country Status (12)

Country Link
US (2) US4971670A (enExample)
EP (1) EP0287513B1 (enExample)
JP (1) JPH0781987B2 (enExample)
CA (1) CA1335805C (enExample)
DE (1) DE3876273T2 (enExample)
DK (1) DK174962B1 (enExample)
ES (1) ES2037273T3 (enExample)
FI (1) FI97893C (enExample)
GR (1) GR3007124T3 (enExample)
HK (1) HK192495A (enExample)
IE (1) IE62803B1 (enExample)
PT (1) PT87210B (enExample)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173160A (en) * 1991-02-27 1992-12-22 Board Of Regents, The University Of Texas System Analysis of carrier ampholytes using immobilized ph gradients
US5323846A (en) * 1992-01-14 1994-06-28 Fotodyne Incorporated Fluid circulator and temperature regulator
GB2267502B (en) * 1992-05-28 1997-01-22 Aligena Ag Polymeric reaction products for use in immobilized buffered gels and membranes
US6129828A (en) * 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US6375899B1 (en) 1993-11-01 2002-04-23 Nanogen, Inc. Electrophoretic buss for transport of charged materials in a multi-chamber system
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US6319472B1 (en) 1993-11-01 2001-11-20 Nanogen, Inc. System including functionally separated regions in electrophoretic system
US20040077074A1 (en) * 1993-11-01 2004-04-22 Nanogen, Inc. Multi-chambered analysis device
US6331274B1 (en) 1993-11-01 2001-12-18 Nanogen, Inc. Advanced active circuits and devices for molecular biological analysis and diagnostics
US5437774A (en) * 1993-12-30 1995-08-01 Zymogenetics, Inc. High molecular weight electrodialysis
US5480526A (en) * 1994-06-07 1996-01-02 Bio-Rad Laboratories, Inc. Methods for the desalting of biological samples: a simple approach to eliminate disturbances in isoelectric focusing caused by the presence of salts
US6403367B1 (en) * 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
US7857957B2 (en) * 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
US6071394A (en) 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
CN1051245C (zh) * 1995-01-20 2000-04-12 清华大学 制备型等电点电泳分离方法及设备
IT1272932B (it) * 1995-01-24 1997-07-01 Pier Giorgio Righetti Reattore ad enzima immobilizzato
US5540826A (en) * 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
US7824532B2 (en) 1995-04-26 2010-11-02 Life Technologies Corporation Apparatus and method for electrophoresis
JP4051421B2 (ja) * 1995-07-05 2008-02-27 サノフイ−アベンテイス・エツセ・ピー・アー 等電点電気泳動によるダルバヘプチド抗生物質の精製
RU2140073C1 (ru) * 1998-01-30 1999-10-20 Стоянов Александр Владимирович Способ формирования сред с заданным профилем ph
DE19831210A1 (de) * 1998-07-03 2000-01-05 Wita Gmbh Wittmann Inst Of Tec Verfahren und Vorrichtung zur zwei-dimensionalen Trennung von Biomolekülen
AUPP521298A0 (en) * 1998-08-12 1998-09-03 Life Therapeutics Limited Purification of fibrinogen
SE9803224D0 (sv) 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method for separation of macromolecules
AUPP790698A0 (en) * 1998-12-23 1999-01-28 Life Therapeutics Limited Separation of microorganisms
US20050224355A1 (en) * 1999-12-23 2005-10-13 Brendon Conlan Removal of biological contaminants
US6267579B1 (en) * 1998-12-23 2001-07-31 Clinical Laboratory Development Group, Inc. Apparatus for making a gradient gel
AUPP790898A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Renal dialysis
AUPP971399A0 (en) 1999-04-12 1999-05-06 Life Therapeutics Limited Separation of plasma components
US6284115B1 (en) 1999-09-21 2001-09-04 Agilent Technologies, Inc. In-line flow through micro-dialysis apparatus and method for high performance liquid phase separations
US6758953B2 (en) 1999-10-28 2004-07-06 Nathan A. Thomas Multistage electrophoresis apparatus and method of use for the separation and purification of cells, particles and solutes
HK1049343A1 (zh) * 1999-11-15 2003-05-09 Proteome Systems Ltd. 多間隔電泳方法及裝置
EP1235634A4 (en) * 1999-11-16 2004-09-01 Proteotools Llc TWO-DIMENSIONAL SEPARATION AND DETECTION OF AMPHOTER SUBSTANCES IN SOLUTION
US7077942B1 (en) * 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
US20080096284A1 (en) * 2000-02-08 2008-04-24 Regents Of The University Of Michigan Protein separation and analysis
WO2001068225A1 (en) * 2000-03-15 2001-09-20 Proteosys Ag Micropreparative isoelectric focussing
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
AUPQ691400A0 (en) * 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
AUPQ697300A0 (en) * 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
US6800184B2 (en) 2000-04-18 2004-10-05 Gradipore, Limited Electrophoresis separation and treatment of samples
GB0010957D0 (en) 2000-05-05 2000-06-28 Novartis Ag Compound & method
US6537434B1 (en) 2000-07-21 2003-03-25 Large Scale Proteomics Corporation First dimension electrophoresis separation method and apparatus
US6562213B1 (en) * 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
US6923896B2 (en) * 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
AUPR051500A0 (en) 2000-09-29 2000-10-26 Proteome Systems Ltd Electrophoresis system
DE60142520D1 (de) * 2000-10-06 2010-08-19 Arturus Capital Ltd Multi-port-elektrophorese-trennvorrichtung und entsprechendes verfahren
AUPR222300A0 (en) * 2000-12-21 2001-01-25 Life Therapeutics Limited Electrophoresis device and method
IL157813A0 (en) * 2001-03-08 2004-03-28 Ethrog Biotechnology Ltd Apparatus and method for electrophoresis
WO2003008977A2 (en) * 2001-07-16 2003-01-30 Protein, Forest, Inc. Arrays of buffers for analysing biomolecules by their isoelectric point
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
US6887362B2 (en) * 2002-02-06 2005-05-03 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices
SI1561756T1 (sl) 2002-09-11 2016-02-29 Chugai Seiyaku Kabushiki Kaisha Postopek čiščenja proteinov
AU2003302418A1 (en) * 2002-11-28 2004-06-18 Arkray Inc. Method and apparatus for concentration and purification of nucleic acid
US7850835B2 (en) * 2003-05-09 2010-12-14 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
AU2004269196B2 (en) * 2003-09-03 2010-03-04 Shmuel Bukshpan Methods and apparatus for rapid crystallization of biomolecules
EP1671112A4 (en) * 2003-10-07 2011-03-23 Life Technologies Corp IMPROVED GELE FOR ISOELECTRIC FOCUSING AND METHOD FOR USE THEREOF
EP1696026A4 (en) * 2003-11-10 2007-05-09 Arkray Inc PROCESS FOR CONCENTRATING AND PURIFYING NUCLEIC ACID AND APPARATUS THEREOF
US20050197496A1 (en) * 2004-03-04 2005-09-08 Gtc Biotherapeutics, Inc. Methods of protein fractionation using high performance tangential flow filtration
US7815783B2 (en) * 2004-03-17 2010-10-19 Bio-Rad Laboratories, Inc. Multi-compartment filter and method of filtering using same
EP1735082A1 (en) * 2004-03-17 2006-12-27 Ciphergen Biosystems, Inc. Materials, methods, and systems for separating and identifying proteins from mixtures
US20050249667A1 (en) * 2004-03-24 2005-11-10 Tuszynski Jack A Process for treating a biological organism
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
WO2006021465A1 (en) * 2004-08-25 2006-03-02 Agilent Technologies, Inc. Electrophoretic separation in a moving fluid
US20060130159A1 (en) * 2004-12-09 2006-06-15 Nick Masiello Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum
WO2006127056A2 (en) * 2005-05-25 2006-11-30 Bio-Rad Laboratories, Inc. Fluidics device
CA2616976C (en) * 2005-07-28 2012-08-28 Bio-Rad Laboratories, Inc. Separation of proteins based on isoelectric point using solid-phase buffers
US7531632B2 (en) * 2006-02-16 2009-05-12 Gtc Biotherapeutics, Inc. Clarification of transgenic milk using depth filtration
WO2007126354A1 (en) * 2006-04-27 2007-11-08 Ge Healthcare Bio-Sciences Ab Electrodic bridge
US20080220442A1 (en) * 2006-12-06 2008-09-11 Proteinics Difference detection methods using isoelectric focusing chips
US8366899B2 (en) * 2007-06-22 2013-02-05 Massachusetts Institute Of Technology Isoelectric focusing systems and methods
CN101896250A (zh) * 2007-10-09 2010-11-24 达尔豪西大学 提纯分子的装置
US8282803B2 (en) 2009-03-25 2012-10-09 Bio-Rad Laboratories, Inc. Isoelectric focusing tray and electrode assembly for alternate gel strip orientations
CN104024396A (zh) * 2011-11-04 2014-09-03 生物辐射实验室股份有限公司 采用pH的电子控制的亲和方法及组合物
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
WO2013067509A1 (en) 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
CA2856779C (en) 2011-11-22 2021-07-20 Stephen G. Haralampu Stopped-flow, m icro-flu i imc device and method for the charge-based separation of complex analyte mixtures
US9658195B2 (en) 2012-02-15 2017-05-23 Bio-Rad Laboratories, Inc. Electronic control of pH and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US9671368B2 (en) * 2013-05-10 2017-06-06 The Regents Of The University Of California Two-dimensional microfluidic devices and methods of using the same
JP2016532100A (ja) 2013-07-05 2016-10-13 ラボラトワール・フランセ・デュ・フラクシオンマン・エ・デ・ビョテクノロジーＬａｂｏｒａｔｏｉｒｅ Ｆｒａｎｃａｉｓ Ｄｕ Ｆｒａｃｔｉｏｎｎｅｍｅｎｔ Ｅｔ Ｄｅｓ Ｂｉｏｔｅｃｈｎｏｌｏｇｉｅｓ アフィニティークロマトグラフィーマトリックス
ES2705700T3 (es) * 2013-07-12 2019-03-26 Hoffmann La Roche Elucidación de la optimización de entrada en cromatografía de intercambio iónico

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3539493A (en) * 1967-08-31 1970-11-10 Canal Ind Corp Apparatus for preparative electrophoresis on gel support media
US3844925A (en) * 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation
SE415731B (sv) * 1977-04-26 1980-10-27 Pharmacia Fine Chemicals Ab Vattenloslig amfolyt for separationsendamal samt sett att framstella densamma
WO1979000002A1 (en) * 1977-06-15 1979-01-11 Nat Res Dev Improvements relating to membrane electrophoresis
EP0103965A2 (en) * 1982-08-20 1984-03-28 Imperial Chemical Industries Plc Electrofocusing apparatus
US5114555A (en) * 1988-01-05 1992-05-19 Monsanto Company Continuous isoelectric separation

Also Published As

Publication number Publication date
GR3007124T3 (enExample) 1993-07-30
PT87210A (pt) 1989-05-12
FI881652A0 (fi) 1988-04-08
PT87210B (pt) 1993-11-30
EP0287513B1 (en) 1992-12-02
IE62803B1 (en) 1995-03-08
FI97893B (fi) 1996-11-29
DK191388A (da) 1988-10-12
JPS63263457A (ja) 1988-10-31
EP0287513A3 (en) 1990-10-10
JPH0781987B2 (ja) 1995-09-06
IE881067L (en) 1988-10-11
FI97893C (fi) 1997-03-10
HK192495A (en) 1995-12-29
US5082548A (en) 1992-01-21
CA1335805C (en) 1995-06-06
DK191388D0 (da) 1988-04-08
EP0287513A2 (en) 1988-10-19
US4971670A (en) 1990-11-20
FI881652A7 (fi) 1988-10-12
DE3876273T2 (de) 1993-05-27
ES2037273T3 (es) 1993-06-16
DE3876273D1 (de) 1993-01-14

Similar Documents

Publication Publication Date Title
DK174962B1 (da) Fremgangsmåde til isoelektrisk fokusering samt apparat til udøvelse af fremgangsmåden
Johansson Agarose gel electrophoresis
Foster et al. Isoelectric focussing behavior of bovine plasma albumin, mercaptalbumin, and β-lactoglobulins A and B
Garfin [35] Isoelectric focusing
Shuster [34] Preparative acrylamide gel electrophoresis: Continuous and disc techniques
US3450624A (en) Apparatus for the separation of chemical components by the combination of electrophoresis and gel filtration
US5589104A (en) Electrophoresis separation gel and a method for preparing an electrophoresis separation gel
JPS58147639A (ja) 連続電気泳動分離法および装置
JP3410099B2 (ja) 担体両性電解質を使用しない等電点電気泳動方法及び装置
Catsimpoolas Isoelectric focusing and isotachophoresis of proteins
US4297198A (en) Concentrating electrophoresis apparatus
RILBE Trends in Instrumental and Methodical Development of Isoelectric Focusing
WO2010017109A1 (en) Systems and methods for quantitative analyte transfer
US20090314639A1 (en) Means and devices for electro-filtration of molecules
Altschul et al. [17] Zone electrophoresis with polyacrylamide gel
GELS HARRY RILBE
Lopez 2-D electrophoresis using carrier ampholytes in the first dimension (IEF)
CN101328206A (zh) 一种非变性的制备性电泳
Hrkal Gel-type techniques
EP1870704A1 (en) Separation medium for biochemical analysis
Låås 12 Electrophoresis in Gels
Leaback et al. Isoelectric focusing of proteins
WO2024157889A1 (ja) 電気泳動により対象物質を処理する方法及び装置
Synge Electrical-Transport Methods.
GB2026546A (en) Displacement electrophoresis

Legal Events

Date Code Title Description
PUP Patent expired