JPH05229915A - Skin external preparation - Google Patents

Abstract

PURPOSE:To provide a skin external preparation good in storage stability, xeroderma-improving effect and corneum-improving effect by adding a lactophorin contained in the proteose peptone of milk protein to a skin external preparation containing an oily substance and water. CONSTITUTION:A skin external preparation is prepared by adding a lactophorin which is a heat-resistant glucoprotein contained in the proteose peptone fraction of mild protein in an amount of 0.1 to 3.0% based on the whole composition to a skin external preparation containing water and an oily substance such as an oil-soluble perfume, oil-soluble vitamin, vegetable fat or oil, or silicone oil. The skin external preparation can be used in the form of cream, emulsion, cosmetic water, gel, etc.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin which is excellent in storage stability and has an effect of improving xerosis (dry skin) and an effect of improving keratin.

[0002]

2. Description of the Related Art Generally, an external emulsifying or solubilizing external preparation for skin such as cosmetics contains a surfactant such as an anionic surfactant or a nonionic surfactant. There is. However, anionic surfactants may be skin irritating, that is, they may enhance transepidermal water loss (loss of water retention). In addition, nonionic surfactants also have problems that they have insufficient emulsifying or solubilizing ability and that they have some skin irritation. Therefore, it is the current situation that the main external preparation for skin is added with a moisturizing agent such as glycerin or propylene glycol in order to maintain water retention. However, there is a problem that the emulsion stability is reduced thereby.

As an emulsifier having low irritation and sufficient water retention to solve such problems, a protein emulsifier in which the amino residue of an amino acid alkyl ester is modified by an amide bond at the carboxyl terminus of a hydrolyzed protein is used. An emulsified cosmetic composition has been developed (Japanese Patent Laid-Open No. Sho 60).
-92205). However, upon detailed examination of this emulsion-type cosmetic composition, it was found that its emulsion stability and water retention are not always sufficient. Therefore, the fact is that an external preparation for the skin which has the effect of improving water retention or xerosis (dry skin) and the effect of improving keratin, and which is excellent in emulsion (solubilization) storage stability has not yet been developed.

[0004]

Means for Solving the Problems The inventors of the present invention have made extensive studies in view of such circumstances, and as a result, the specific external preparation for skin has the storage stability and further has the effect of improving xeroderma (dry skin). , And a composition having an effect of improving keratin, and have completed the present invention.

That is, an object of the present invention is to provide a skin external preparation having excellent emulsion (solubilization) storage stability and an effect of improving xeroderma (dry skin) and keratin. The above-mentioned object is achieved by an external preparation for skin which is characterized by containing an oily substance and water and containing lactophorin in the milk protein proteose peptone.

The structure of the present invention will be described in detail below. Lactophorin used in the present invention is a known thermostable glycoprotein present in the proteose peptone fraction of whey (Journal of the Society of Animal Science, Vol. 61, No. 11, 965-97).
P. 7, 1990).

[0007] Lactofolin contains skim milk at 95 ° C for 20
After heating for 30 minutes and cooling to 30 ° C., one of the supernatants of the non-dialysable substance obtained by removing the casein and the heat-denatured protein that precipitates at pH 4.6 was analyzed by DEA.
E cellulose column chromatography (pH 7.
7), Sephadex G-100GPC column chromatography, and further gel filtration of Biogel A-15m.

[0008] Lactofolin can be obtained not only from milk thus heat-treated, but also from unheated milk. That is, after reducing the ammonium sulfate fraction of whey protein with mercaptoethanol, Sepharose GL-6
It can also be obtained by sequentially performing B, then DEAE cellulose column chromatography and high performance liquid chromatography.

According to the structural analysis of lactophorin, lactophorin contains 18% sugar, and the constituent sugars are fucose, mannose, galactose, N-acetylgalactosamine, N-acetylglucosamine and sialic acid. Further, its constituent amino acids are characterized in that hydrophobic amino acids such as threonine, proline, alanine, cystine, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, and arginine are about 60%, and aspartic acid, glutamic acid, It also includes hydrophilic amino acids such as lysine, histidine, and arginine.

Lactophorin has the characteristic of reacting with the antibody of the soluble glycoprotein of the milk fat globule membrane of milk.

As the lactophorin used in the present invention, the crude lactophorin obtained by fractionating the proteose peptone fraction of whey can be used as it is as an external preparation for the skin. An excellent effect can be obtained.

The content ratio of lactophorin in the external preparation for skin of the present invention is about 0.01 to 5%, preferably 0.1 to 3.0%, based on the total amount. Content rate 0.01
If it is less than%, it is difficult to obtain sufficient emulsifying property and storage stability, which are one of the objects of the present invention. Further, even if the content ratio exceeds 5.0%, it is difficult to obtain the improvement of the characteristics commensurate with the increase, and good sensory characteristics are not exhibited.

The oily substance used in the present invention includes all common oily substances, and the range thereof can be widely used from polar oil to non-polar. That is, oil-soluble flavors, oil-soluble vitamins, oil-soluble hormones, oil-soluble ultraviolet absorbers,
Higher aliphatic hydrocarbons, vegetable oils and fats, animal oils and fats,
Examples thereof include waxes, higher fatty acids, higher alcohols, synthetic ester oils and silicone oils.

More specifically, examples of the oil-soluble flavor include oil-soluble flavors extracted from natural animals and plants and synthetic oil-soluble flavors. Examples of the oil-soluble vitamins include vitamin A, vitamin D, vitamin E, vitamin F, vitamins of vitamin K group, pyridoxine dicaprylate, pyridoxine dipalmitate, dl-α-tocopherol acetate, dl-α-tocopherol nicotinate, Examples include vitamin derivatives such as ascorbyl dipalmitate, ascorbyl monopalmitate, and ascorbyl monostearate. Examples of the oil-soluble hormones include estradiol, ethinyl estradiol, estrone, diethylstilbestrol and the like, and examples of the oil-soluble pigments include sudan III, tetrabrom fluorescein, dibrom fluorescein, fluorescein, quinizarin green SS and the like, oil-soluble hormones. Examples of the ultraviolet absorbers include oxybenzone and methyl 2,5-diisopropylcinnamate.

Examples of the higher aliphatic hydrocarbons include:
Liquid paraffin, squalane, microcrystalline wax, vaseline, ceresin and the like, vegetable oils and fats, for example, olive oil, castor oil, cocoa butter, palm oil and the like, animal oils and fats, for example, cod liver oil, beef tallow, butter. Fats, waxes, for example, beeswax, carnauba wax, etc., higher fatty acids, for example, lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, behenic acid, lanolin fatty acid, etc., higher alcohols, For example, synthetic ester oils such as lauryl alcohol, stearyl alcohol, cetyl alcohol, oleyl alcohol, etc. include, for example, butyl stearate, hexyl laurate, octyldodecyl myristate, diisopropyl adipate, diisopropyl sebacate, etc. Include a branched chain ester. These oily substances are used alone or in combination of two or more.

The dosage form of the external preparation for skin of the present invention is not particularly limited, and examples thereof include cream, emulsion, lotion and gel.

[0017]

EXAMPLES The present invention will be described below with reference to examples.
In the present invention, all "%" mean "% by weight". Prior to the examples, evaluation items and evaluation methods will be described. The properties of the external preparation for skin examined in the examples are appearance, storage stability, permeability, pH, dry skin improving effect and keratin improving effect.

(1) With respect to the appearance, the colored state and the like were judged with the naked eye. (2) The storage stability of the sample composition was 45 ° C, 40 ° C, 3 ° C.
It was left in a constant temperature bath of 0 ° C., 5 ° C. or 0 ° C. for 3 to 10 months, and its viscosity or the presence or absence of precipitate was inspected. (3) The transmittance represents the transmittance of light having a wavelength of 450 nm. When the value is 80% or more, it is clear to the naked eye. (4) The pH was measured immediately after adjusting the cosmetics. After adjusting the pH measuring instrument with a glass electrode with the pH standard solution, p
H was measured.

(5) Test Method for Measuring Dry Skin Improvement Effect The effect of continuous application for one week was examined on 20 middle-aged and elderly subjects having dry skin on their lower legs. About 1 g of the sample was applied to the left lower leg test site of the test subject once a day, and the condition of the skin before and after the start of the test was evaluated according to the following criteria.
The right lower leg served as a control without applying the sample. The judgment results of the test site before and after the test and the control site are compared, and when the skin dryness is improved by two or more steps (for example, + →-, ++ → ±), it is “effective”, and when it is improved by one step, it is “slightly”. "Valid" and "No" when there was no change. In addition, there was no case that the skin became dry during the test period.

(6) Method for measuring keratin improving (increasing anti-peeling property of keratinocytes) Scotch tape (Nichiban Mending Tape) is adhered to the skin of the test area where the dry skin improving effect measuring test is conducted. The state of the keratinocytes attached to the tape when peeled off was examined in detail by a scanning electron microscope, and the skin corneocyte anti-peeling property was classified according to the following criteria, and the keratin improving effect was evaluated. The evaluation was "effective" when the difference between the evaluation points of the test site after application for 2 weeks and that of the control site was 2 points or more, "slightly effective" when 1 point, and "ineffective" when 0 point. .. There were no cases where the evaluation score of the test site was higher than that of the control site.

Examples 1 to 5 and Comparative Example 1 The emulsions of Examples 1 to 5 and Comparative Example 1 were prepared according to the formulations shown in Table 1. Oily substances 1 to 4 as components in the table were uniformly mixed and dissolved at about 80 ° C (I). The water-soluble components 5 to 8 of the components in the table were uniformly dissolved at about 80 ° C (II). Next, while stirring (II) with a homomixer, (I) was added to emulsify, the mixture was cooled to 30 ° C., and stirring was stopped.

The characteristics of the emulsion obtained are shown in Tables 1 and 2.
As can be seen from Table 1, the emulsions of Examples 1 to 5 are better in storage stability and appearance than the emulsion of Comparative Example 1. Further, as can be seen from Table 2, the emulsions of Examples 1 to 5 are more excellent in the dry skin improving effect and the keratin improving effect.

[0023]

[Table 1]

[0024]

[Table 2]

Example 6 (Toner lotion) A. Prescription ──────────────────────────── 1. Ethanol 10.0% 2. Bergamot oil 0.05 3. Dipropylene glycol 5.0 4. Potassium dihydrogen phosphate 0.06 5. Sodium monohydrogen phosphate 0.04 6. Lactofolin 0.4 7. Purified water 84.45

B. Manufacturing Method The above components 1 to 3 are dissolved, and the components 4 to 7 dissolved therein are mixed to prepare a lotion.

The lotion of Example 6 thus obtained was 45
The stability was good when stored for 4 months in a constant temperature bath at ℃. It was also good after storage at 5 ° C for 6 months.

The transparency (permeability) of this lotion was 88% and 8% immediately after production and after 3 months at 30 ° C., respectively.
It had a visually transparent appearance at 2%. The pH was 6.2. Further, Table 1 shows (1) dry skin improving effect and (2) keratin improving effect of the present lotion, and the effects were more excellent.

Example 7 (Cream) A. Prescription ──────────────────────────── 1. Olive oil 5.0% 2. Squalane 15.0 3. Beeswax 3.0 4. Cetyl alcohol 7.0 5. Glyceryl monostearate 3.0 6. White vaseline 3.0 7. Crude Lactofolin 2.0 (Purity 60%) 8. Maltitol solution 10.0 9. Methyl paraoxybenzoate 0.2 10. Perfume 0.1 11. Purified water 51.7

B. Manufacturing method The above components 1 to 6 are dissolved by heating and maintained at about 80 ° C (I). On the other hand, the above components 7 to 9 and 11 are melted by heating and maintained at about 80 ° C. (II). Next, (II) is added with stirring with a homomixer to emulsify and then cooled. In addition, component 10 was added at 70 ° C. during the cooling process, and then 30 ° C.
And cooled further until the stirring was stopped. The obtained cream is an O / W type emulsion, and it is 4
When stored for months, the stability was extremely good. Further, it was extremely stable even after being stored at 0 ° C. and 5 ° C. for 6 months. No change in quality or odor occurred at any temperature. The appearance was also good. Table 1 shows (1) dry skin improving effect and (2) keratin improving effect, which were excellent.

[0031]

[Table 3]

[0032]

As described above, the present invention provides storage stability,
It is obvious to provide a skin external preparation that is excellent in dry skin improving effect and keratin improving effect.

─────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] February 23, 1993

[Procedure Amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0016

[Correction method] Change

[Correction content]

The external preparation for skin of the present invention may contain the following components in addition to the above-mentioned components within a range that does not impair the effect. As the anionic surfactant, a carboxylic acid group,
Anionic surfactants having one or more kinds of sulfonic acid group, sulfuric acid ester group, and phosphoric acid ester group in the molecule are mentioned. Fatty acid soaps, ether carboxylic acids and salts thereof, carboxylic acid salts such as condensates of amino acids and fatty acids, and the like having a carboxylic acid group, and those having a sulfonate include alkyl sulfonates,
Sulfosuccinic acid, ester sulfonates, alkylallyl and alkylnaphthalene sulfonates, N-acyl sulfonates, formalin condensed sulfonates, and the like having a sulfate ester group include sulfated oil, ester sulfates, Alkyl sulfates, ether sulfates, alkyl allyl ether sulfates, amide sulfates and the like, and those having a phosphoric acid ester group include alkyl phosphates, amide phosphates, ether phosphates, alkyl allyl ether phosphates. Etc. can be mentioned. Examples of the amphoteric surfactant include alkyl betaine, alkylamido betaine, alkyl imidazolinium betaine and the like. Examples of the semipolar surfactant include dimethyllaurylamine oxide, dimethylmyristylamine oxide, dimethylcetylamine oxide, dimethylstearylamine oxide, dimethyloleylamine oxide, dimethylbehenylamine oxide, and methyldilaurylamine oxide, and cationic surfactants. As a fatty acid amine salt,
Examples thereof include an alkyl quaternary ammonium salt, an aromatic quaternary ammonium salt, a pyridinium salt, an imidazolium salt and the like.
As the lipophilic nonionic surfactant, for example, sorbitan monooleate, sorbitan monoisostearate,
Sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, penta-2-ethylhexyl diglycerol sorbitan, tetra-2
-Sorbitan fatty acid esters such as diglycerol sorbitan ethylhexylate, mono-cotton oil fatty acid glycerin, glyceryl monoerucate, glyceryl sesquioleate, glyceryl monostearate, α, α'-glyceryl pyroglutamate oleate, glyceryl monostearate malic acid Glycerin polyglycerin fatty acid, propylene glycol fatty acid ester such as propylene glycol monostearate, hydrogenated castor oil derivative, glycerin alkyl ether and the like. Examples of the hydrophilic nonionic surfactant include POE sorbitan monooleate, POE-sorbitan monostearate, POE-sorbitan monooleate, POE sorbitan tetraoleate and other POE sorbitan fatty acid esters, POE sorbit monolaurate, POE sorbit monooleate, POE sorbit pentaoleate, POE sorbit monostearate, and other POE sorbit fatty acid esters, POE glycerin monostearate, POE glycerin monoisostearate. P
POE glycerin fatty acid esters such as OE glycerin triisostearate, POE monooleate, POE
POE fatty acid esters such as distearate, POE dioleate, ethylene glycol distearate, PO
E lauryl ether, POE oleyl ether, POE
Stearyl ether, POE behenyl ether, POE
POE alkyl ethers such as 2-octyldodecyl ether, POE cholestanol ether, POE octyl phenyl ether, POE nonyl phenyl ether,
POE alkyl phenyl ethers such as POE dinonyl phenyl ether, Pluronic types such as Pluronic, POE / POP cetyl ether, POE / POP2
-Decyl tetradecyl ether, POE / POP monobutyl ether, POE / POP hydrogenated lanolin, POE /
POE / POP alkyl ethers such as POP glycerin ether, tetra-POE / tetra-P such as Tetronic
OP ethylenediamine condensates, POE castor oil, PO
E hydrogenated castor oil, POE hydrogenated castor oil monoisostearate, POE hydrogenated castor oil triisostearate, PO
E hydrogenated castor oil monopyroglutamic acid monoisostearate diester, POE hydrogenated castor oil maleic acid, etc. P
OE-castor oil derivative, POE beeswax lanolin derivative such as POE sorbit beeswax, coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, alkanolamides such as fatty acid isopropanolamide, P
Examples thereof include OE propylene glycol fatty acid ester, POE alkylamine, POE fatty acid amide, sucrose fatty acid ester, glucose alkyl ether, POE nonylphenylformaldehyde condensate, alkylethoxydimethylamine oxide, and trioleylphosphoric acid.
As the clay mineral, for example, montmorillonite, zakonite, nontronite, saponite, hectrite,
Examples thereof include natural and synthetic water-swelling clay minerals such as vermiculite, bee gum, bentonite, silicate, fluorosilicate, magnesium, aluminum and synthetic hectorite (laponite). Examples of natural water-soluble polymers include gum arabic, gum tragacanth,
Plant-based polymers such as galactan, guar gum, carob gum, karaya gum, carrageenan, pectin, agar, quince seed (quince), algecoloid (cassius extract), starch (rice, corn, potato, wheat), locust bean gum, glycyrrhizic acid, etc., Examples thereof include microbial polymers such as xanthan gum, dextran, succinoglucan and pullulan, and animal polymers such as collagen, casein, albumin and gelatin. As the semi-synthetic water-soluble polymer, for example, carboxymethyl starch, starch-based polymers such as methyl hydroxypropyl starch, methyl cellulose, nitrocellulose, ethyl cellulose, methyl hydroxypropyl cellulose, hydroxyethyl cellulose, sodium cellulose sulfate, hydroxypropyl cellulose, Examples thereof include cellulosic polymers such as sodium carboxymethyl cellulose (CMC), crystalline cellulose and cellulose powder, and alginic acid polymers such as sodium alginate and propylene glycol alginate. Examples of the synthetic water-soluble polymer include polyvinyl alcohol, polyvinyl methyl ether, polyvinylpyrrolidone, carboxyvinyl polymer (Carbopol), vinyl polymers such as sodium polyacrylate, polyethylene glycol 20,000, 4,000,000. , 600,00
0 and other polyoxyethylene-based polymers, polyoxyethylene-polyoxypropylene copolymers and other copolymer-based polymers,
Sodium polyacrylate, polyethyl acrylate,
Examples thereof include acrylic polymers such as polyacrylamide, polyethyleneimine, and cationic polymers. Examples of the inorganic water-soluble polymer include bentonite, magnesium aluminum silicate (veegum), laponite, hectorite, and anhydrous silicic acid. Examples of the organic amine include monoethanolamine, diethanolamine, triethanolamine, morpholine, triisopropanolamine, 2-amino-2-methyl-1,3-
Examples include propanediol and 2-amino-2-methyl-1-propanol. Examples of the inorganic powder include talc, titanium oxide, mica, kaolin, silicic acid anhydride, silicates such as magnesium silicate, zinc oxide, magnesium oxide, zirconium oxide, calcium carbonate, magnesium carbonate, mica, titanium mica, and sericite. , Nylon powder, polyethylene powder, cellulose powder, acrylic resin and the like. As the inorganic pigment, for example, red iron oxide, yellow iron oxide, black iron oxide, ultramarine blue, titanium coated mica, bismuth oxychloride, binder pigment, Gunzhou pink, chromium oxide, aluminum oxide cobalt, navy blue,
Examples include black iron oxide, carbon black, and calamine. Examples of the polysaccharide include sodium chondroitin sulfate, sodium hyaluronate and the like. Examples of amino acids include glycine, alanine, valine, leucine, isoleucine, serine, threonine, phenylalanine, tyrosine, tryptophan, cystine, cysteine, methionine, proline, hydroxyproline, L-
Examples thereof include neutral amino acids such as Dopa, acidic amino acids such as aspartic acid, glutamic acid, asparagine and glutamine, and basic amino acids such as araginine, histidine and lysine. Examples of the amino acid derivative include sodium acylsarcosine (sodium lauroylsarcosine), acylglutamate, sodium β-alanine acyl, glutathione, and pyrrolidonecarboxylic acid. The dosage form of the external preparation for skin of the present invention is not particularly limited, and examples thereof include cream, emulsion, lotion and gel.

Claims (1)

[Claims] 1. A skin external preparation containing an oily substance and water and lactophorin contained in milk protein proteose peptone in the skin external preparation.