JPH05168346A - Method for indoor cultivation of lyophyllum decastes sing. - Google Patents

Abstract

PURPOSE:To efficiently carry out the indoor cultivation of Lyophyllum decastes Sing. enabling the harvesting of a large amount according to simple operation by spreading an agar residue over the interior of a vessel having a structure for keeping a culture medium in wide contact with air and embedding a mushroom bed therein. CONSTITUTION:An agar residue which is a mixture of a hot water-insoluble substance obtained in a process for producing agar with perlite, etc., used as a filter aid is spread over the interior of a vessel having a structure for keeping a culture medium in wide contact with air. A mushroom bed is then embedded therein to carry out the indoor cultivation of Lyophyllum decastes Sing.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a method for indoor cultivation of Hatake shimeji mushrooms. More specifically, the agar residue is spread on a container having a structure in which a culture medium is in wide contact with air, and a fungal bed is embedded therein for cultivation. This is an indoor cultivation method of Hatake shimeji mushroom that can increase the yield per culture medium.

[0002]

2. Description of the Related Art Hatake shimeji mushrooms are mushrooms of the genus Shimeji, whose fruiting body is similar in form to honshimeji, and are delicious enough to be said to be a saprophytic type of honshimeji, and they are edible mushrooms having a good aroma and texture. Mushrooms are a type of saprophytic mushroom, and in autumn, they occur in large numbers in forests, gardens, fields, roadsides, and sometimes under the floors of houses (Rokuya Imaseki, Tsugio Hongo: New Japanese fungi of primary color). Picture book (I), nursery school, 1987).

As a method for cultivating this mushroom indoors,
Oxygen scraps or bark compost is filled with rice bran, poultry manure, mulch, ash, etc. in a cultivation bag or polypropylene culture bottle, which is inoculated with inoculum to control room temperature and humidity at a constant temperature in a room. It is generally cultivated. Among the cultivation methods as described above, the method of using the cultivation bag includes culturing the inoculum in the cultivation bag, and when the hyphae are completely infested in the bag and the primordia formation of fruiting bodies is observed. It is known that the upper part of the bag is cut and the opening is covered with vermiculite for about 1 cm to generate fruiting bodies (Fukushima Prefectural Forestry Research Institute report, 17: 95-96, 198).
4) In this method, 7
It has the drawback of taking ~ 8 months.

As a method using a cultivation bottle, after culturing the mycelium for a certain period of time, the fungus is scratched, chilled water is irrigated and left standing for a whole day and night, then excess water is discarded, and cultivation is continued again and fruit bodies are continued. There is known a method for generating the above (Japanese Patent Laid-Open No. 63-169913). However, this method is also
Although harvesting is possible in 50 to 60 days, it has the drawback that the occurrence of fruiting bodies is uncertain and reproducibility is poor.

In addition to these methods, according to the present inventors, a mixture of bark compost and rice bran is used as a culture medium, and when the mycelium spreads in the cultivation container, a mineral substance composed of fine particles. There is a method of cultivating by covering the opening of the cultivating container (Japanese Patent Laid-Open No. 3-244320) or a method of culturing by burying a matured bacterial bed in a vat-shaped container filled with a mineral substance composed of fine particles (special Wishhei 3-8000).

[0006]

As described above, although there are many reports on the method of indoor cultivation of Hatake shimeji, most of them have the above-mentioned drawbacks,
It has not reached the stage where it can be used industrially. However, the method described in Japanese Patent Application No. 3-8000, which was previously filed by the present inventors, until now, in a container having a structure in which the culture medium is in wide contact with air, has a good bamboo shoots due to contamination of various bacteria. What was said to be difficult to grow,
This has been solved by embedding a bacterial bed in a mineral substance consisting of fine particles, which enables large-scale cultivation of high-quality Hatake shimeji mushrooms and is effectively used industrially.

However, in this method, the substance contained in the vat-shaped container is narrowly limited to "mineral substance consisting of fine particles". For example, when it becomes difficult to obtain this substance, It may be difficult to carry out the invention. Therefore, it has been desired to develop a method using a substance other than the "mineral substance consisting of fine particles".

The object of the present invention is to use the agar residue, which is an organic substance that is completely different from the mineral substance consisting of fine particles, so that even if the culture medium has a structure in which the culture medium is in wide contact with air, the mycelium of Hatake shimeji is influenced by various bacteria. It enables to grow well without being affected by it, so that when cultivating a large amount as in the conventional case, the complexity of using many containers with a narrow contact surface between the culture medium and air is eliminated, and the fruiting body per culture medium is eliminated. It is to provide an indoor cultivation method of Hatake shimeji mushroom, which has a large yield.

[0009]

Means for Solving the Problems The inventors of the present invention have conducted earnest research on a method of cultivating Hatake shimeji using a container having a structure in which a culture medium is in wide contact with air, and as a result, spread agar residue on the container. The present invention has been completed by finding that, when the fungus bed is embedded and cultivated, the mushrooms are satisfactorily grown without being affected by various bacteria.

[0010] That is, the present invention is an indoor cultivation method for Haetake shimeji mushroom characterized in that agar residue is spread in a container having a structure in which a culture medium is in wide contact with air, and a fungus bed is embedded therein for cultivation. The present invention will be described in detail below. The indoor cultivation method of Hatake shimeji mushroom in the present invention is usually performed by the following method.

A vessel for producing a bacterial bed is filled with a culture medium for producing a bacterial bed, which is sterilized by heating, cooled, and inoculated with a pre-produced inoculum at room temperature 20 to 25 ° C. and 60 to 80 ° C.
Culturing is performed in a room adjusted to 100% to prepare a bacterial bed. Next, spread agar residue with a thickness of 1 to 5 cm in a cultivation container for mushroom production, embed the aforesaid fungal bed in it, and cover the agar residue with a thickness of 1 to 5 cm on it. ~ 20
When cultivation is continued in a room adjusted to ℃, humidity of 90 to 95%, and illuminance of 50 to 300 lux, it will be 20 to 40 after implantation of the fungal bed.
The fruit body can be harvested in a day. Cultivation Container for Mushroom Generation In the present invention, a container having a structure in which a culture medium is in wide contact with air is used as a cultivation container for mushroom generation. A container having a structure in which the culture medium is in wide contact with air is a container in which conventionally used cultivation methods are affected by various bacteria because the culture medium is in wide contact with air, and the mycelium of Hataketake shimeji cannot grow well. Say. Specifically, for example, one having a shape like a bat may be mentioned. The material is not particularly limited, and plastics such as acrylic, vinyl chloride, and polycarbonate, aluminum, copper,
It can be widely used for metals such as iron, glass, wood, stone, and ceramics. Agar residue used in agar residues present invention mainly MAXA, Gracilaria, Obakusa, Ooogonori, the hot water-insoluble material obtained in preparing the agar seaweed such Itanigusa as a raw material,
It is a mixture of perlite or the like used as a filter aid, and may be either fermented and decomposed or unfermented.
The agar residue has appropriate air permeability and water retention property, which is thought to prevent the support in the culture medium from being dried and the growth of bacteria to be able to favorably grow Haetake shimeji hyphae. The sawdust used conventionally has good air permeability, but since the water retention is poor, the support is dried and the growth of mycelia is hindered, and the bark compost has good water retention, Since it is inferior in nature, it is easily contaminated with bacteria,
It seems that the growth of mycelium is inhibited. Some of the agar residues produced in this way are registered under the trademark "Agarpost" (1990 trademark application No. 141952). The detailed manufacturing process of agar and agar residue is shown in FIG. The culture medium for producing the bed is a bark compost, sawdust and agar residue alone or a mixture of these and rice bran, preferably mixed in a volume ratio of 2: 1 to 5: 1 to obtain a water content. It is better to use the one prepared to 60 to 70%. Further, if necessary, a mixture of organic components such as chicken manure and mulch can be used. As the cultivation container for producing a fungal bed, any cultivation container generally used for artificial cultivation of mushrooms can be used as long as the fungal bed can be removed. Usually, it is preferable to use a polypropylene bottle or a rectangular parallelepiped bag having a capacity of 800 to 1000 ml. Heat Sterilization Method The heat sterilization method can be performed by an autoclave as is generally performed. Usually, sterilization may be performed at a temperature of 120 to 130 ° C for 2 to 3 hours, but in some cases, heat sterilization is performed once, a certain period of time is allowed to elapse, and then heat sterilization is performed again. May be. Preparation method of inoculum To prepare inoculum, an ordinary method may be used. Aseptic mycelia obtained by inoculating bark compost or sawdust and rice bran in a volume ratio of 2: 1 to 5: 1 and inoculating a medium prepared to a water content of 60 to 70%, 20 to
It is obtained by culturing at 25 ° C for about 20 days. In addition,
The medium used for tissue culture and subculture can generally be any medium as long as basidiomycete grows,
For example, any of the culture mediums described in "Fungus Research Method" (Kiyo Aoshima, Keisuke Tsubaki, Koichi Miura; P398-408, published June 1, 1983, Kyoritsu Publishing) can be used, but particularly preferred examples Is a medium having the composition shown in Table 1 or Table 2.

Hereinafter, the present invention will be described in more detail with reference to examples.

[0013]

Example 1 An agar residue and bark compost were mixed in an equal amount (volume), and rice bran was further mixed in a volume ratio of 3: 1 to prepare a culture medium having a water content of 65%. About 800 g was filled in a polypropylene cultivation bag. Then, the bag mouth was made into a double woven fabric, and the stapler (trademark) was used to stop at 3 places, and this was sterilized by autoclaving at 120 ° C. for 3 hours. After allowing the temperature of the culture medium to cool to 25 ° C or lower, 15 g of inoculum was inoculated in a clean bench and cultured for 60 days in a room adjusted to room temperature of 23 ° C and humidity of 70%. As a result, the hyphae were sufficiently spread in the cultivation bag in 30 days, and the cultivation was continued for another 30 days.
Then, the agar residue with a water content of 65% was laid on the inner bottom of a bat-shaped container of 60 cm in width × 20 cm in length × 18 cm in depth to a thickness of 3 cm and the bag was removed there 10 cm in width × 5 cm in length × 10 cm in depth. Bed size 4
I put the pieces. Furthermore, agar residue with a water content of 65% was coated on this to a thickness of 3 cm from the upper surface of the fungus bed, and the room temperature was 17 ° C and the humidity was 95%.
%, The cultivation was continued in a room where the illuminance was adjusted to 80 lux, and water was sprayed at appropriate times. As a result, on the 30th day after the coating with the agar residue, 500 g of fruit bodies of Hatake shimeji mushroom were collected.

[0014]

[Example 2] Cultivation was performed in the same manner as in Example 1 except that the agar residue and bark compost were used alone as the culture medium for producing the bacterial bed. As a result, fruit bodies were collected as in the case of Example 1.

[0015]

EFFECTS OF THE INVENTION According to the present invention, even if a vat-shaped container is used, the mushroom mycelia can grow well without being influenced by bacteria. Therefore, as in the conventional case, for large-scale cultivation, the complexity of using a large number of containers with a narrow contact surface between the culture medium and air is eliminated, and the yield of fruiting bodies per culture medium is also improved, with a simple operation, in large quantities. Hatshimejimeji can be cultivated.

[0016]

[Table 1] Examples of tissue culture and passage medium

[0017]

[Table 2] Examples of tissue culture and passage medium

[Brief description of drawings]

FIG. 1 shows a process for producing agar and an agar residue.

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Masaki Ito 24-9 Nozono-cho, Kameyama-shi, Mie Oji Paper Co., Ltd. Forest Tree Breeding Institute Kameyama Laboratory

Claims (1)

[Claims] 1. A method for indoor cultivation of Hatake shimeji mushrooms, which comprises cultivating an agar residue by laying it in a container having a structure in which a culture medium is in wide contact with air, and burying a fungal bed therein.