KR102663030B1 - Medium composition for culturing morel and the culturing method for morel thereby - Google Patents
Medium composition for culturing morel and the culturing method for morel thereby Download PDFInfo
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- VZSRBBMJRBPUNF-UHFFFAOYSA-N 2-(2,3-dihydro-1H-inden-2-ylamino)-N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]pyrimidine-5-carboxamide Chemical compound C1C(CC2=CC=CC=C12)NC1=NC=C(C=N1)C(=O)NCCC(N1CC2=C(CC1)NN=N2)=O VZSRBBMJRBPUNF-UHFFFAOYSA-N 0.000 description 1
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- 229910052732 germanium Inorganic materials 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Mushroom Cultivation (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Wood Science & Technology (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
본 발명은 곰보버섯의 재배용 배지 조성물 및 곰보버섯의 재배방법에 관한 것으로서, 본 발명의 배지 조성물을 이용하여 배양할 경우, 자실체 배양이 어려웠던 곰보버섯의 자실체를 배양할 수 있으며, 또한, 본 발명의 배지 조성물을 이용할 경우, 노지재배, 하우스재배 또는 상자재배 등 재배방법에 관계없이 곰보버섯의 생장 효율이 우수하여 곰보버섯을 저비용으로 대량생산이 가능하고, 생산비 절감 효과가 있다.The present invention relates to a culture medium composition for the cultivation of morel mushrooms and a method for cultivating morel mushrooms. When cultured using the medium composition of the present invention, the fruiting bodies of morel mushrooms, which were difficult to cultivate, can be cultivated. In addition, the present invention When using a medium composition, the growth efficiency of morel mushrooms is excellent regardless of the cultivation method such as open field cultivation, house cultivation, or box cultivation, making it possible to mass produce morel mushrooms at low cost and reducing production costs.
Description
본 발명은 곰보버섯 재배용 배지 조성물 및 이를 이용한 곰보버섯의 재배방법에 관한 것이다.The present invention relates to a culture medium composition for morel mushroom cultivation and a method for cultivating morel mushrooms using the same.
전세계 버섯의 종류는 약 15,000여종이며, 먹을 수 있는 버섯은 1,000∼2,000종으로 알려져 있으며, 국내 자생 버섯류는 약 1,500여종으로 식용은 350여종, 독버섯은 약 90여종 알려져 있고, 국내에 상업적으로 재배되는 버섯 종류는 약 30여종이며, 주로 느타리, 양송이, 팽이, 큰느타리, 표고, 영지버섯 등의 비중이 95% 이상으로 집중되어 있으며, 기후변화와 웰빙, 기능성 등 소비자 트렌드 변화에 따른 다양한 새로운 버섯 품목의 개발과 이에 대한 연구가 요구되고 있으나, 국내에서는 거의 이루어지지 않고 있다.There are about 15,000 types of mushrooms around the world, and 1,000 to 2,000 types of edible mushrooms are known. There are about 1,500 types of native mushrooms in Korea, of which 350 are edible and about 90 types of poisonous mushrooms are known. There are about 30 types of mushrooms, mainly oyster mushrooms, button mushrooms, enoki, large oyster mushrooms, shiitake mushrooms, and reishi mushrooms, which account for more than 95%, and various new mushroom items in response to changes in consumer trends such as climate change, well-being, and functionality. Development and research on this are required, but little is being done in Korea.
곰보버섯은 세계에서 가장 희귀한 식용균의 하나이며, 고급 웰빙 영양식품으로 높은 유기 게르마늄(Ge) 함유로 신장보호 및 정신을 맑게 하고, 성기능 쇠약, 위장염， 소화불량， 식욕부진, 암, 감기예방효과, 면역력 증가 등에 효과가 있으며, 다양한 비타민 및 아미노산이 포함되어 있고, 단백질 함량은 목이버섯의 약 2배, 표고버섯의 1.5배이다.Morel mushrooms are one of the rarest edible fungi in the world, and are a high-quality wellness nutritional food. They contain high organic germanium (Ge), which protects the kidneys, clears the mind, and prevents sexual dysfunction, gastroenteritis, indigestion, loss of appetite, cancer, and colds. It is effective in increasing immunity, contains various vitamins and amino acids, and its protein content is about twice that of wood ear mushrooms and 1.5 times that of shiitake mushrooms.
곰보버섯의 인공재배는 1883년에 영국, 미국, 프랑스 및 독일 등에서 시작하였지만, 자실체의 대량재배는 모두 실패하였으며, 1958년 발효탱크에서 처음으로 곰보버섯의 균사체를 성공적으로 인공배양 하였지만 그 후에 30년간 계속 균사체생산단계에서 멈추었다.Artificial cultivation of morel mushrooms began in England, the United States, France, and Germany in 1883, but all mass cultivation of fruiting bodies failed. In 1958, mycelium of morel mushrooms was successfully artificially cultured for the first time in fermentation tanks, but it continued for 30 years thereafter. It continued to stop at the mycelium production stage.
곰보버섯은 특별한 풍미를 가지므로 프랑스와 이태리에서 고급요리에 이용하며, 유럽과 미국에서는 음식재료, 초콜릿, 와인 등에 폭넓게 활용하고 있으며, 양질의 웰빙 먹거리 확보와 고부가가치 농산물 생산을 통한 농가소득 증대 위해 곰보버섯의 대량 재배기술 개발에 대한 연구는 매우 필요하다.Morel mushrooms have a special flavor, so they are used in high-end cuisine in France and Italy. In Europe and the United States, they are widely used in food ingredients, chocolate, and wine. They are used to secure quality healthy food and increase farm income through the production of high value-added agricultural products. Research into the development of mass cultivation technology for morel mushrooms is very necessary.
이에 본 발명자들은 자실체 배양이 어려웠던 곰보버섯의 자실체를 배양할 수 있는 배지 조성을 확인하고 본 발명을 완성하였다.Accordingly, the present inventors confirmed the composition of a medium capable of cultivating the fruiting bodies of morel mushrooms, which were difficult to cultivate, and completed the present invention.
본 발명의 목적은 곰보버섯 재배용 배지 조성물을 제공하는 것이다.The purpose of the present invention is to provide a medium composition for morel mushroom cultivation.
본 발명의 다른 목적은 곰보버섯 균사체의 재배방법을 제공하는 것이다.Another object of the present invention is to provide a method for cultivating morel mushroom mycelium.
본 발명의 또다른 목적은 상기 재배방법에 의해 재배된 곰보버섯 균사체를 제공하는 것이다.Another object of the present invention is to provide morel mushroom mycelium grown by the above cultivation method.
본 발명의 다른 목적은 곰보버섯 자실체의 재배방법을 제공하는 것이다.Another object of the present invention is to provide a method for cultivating morel mushroom fruiting bodies.
본 발명의 또다른 목적은 상기 재배방법에 의해 재배된 곰보버섯 자실체를 제공하는 것이다.Another object of the present invention is to provide morel mushroom fruiting bodies grown by the above cultivation method.
상기 목적을 달성하기 위하여,In order to achieve the above purpose,
본 발명은 참나무 발효톱밥;The present invention relates to fermented oak sawdust;
부엽토; 및Humus; and
밀, 왕겨 및 옥수수가루로 이루어진 군으로부터 선택되는 하나 이상;을 포함하는 것을 특징으로 하는 곰보버섯 재배용 배지 조성물을 제공한다.It provides a medium composition for morel mushroom cultivation, comprising at least one selected from the group consisting of wheat, rice husk, and corn flour.
또한, 본 발명은 톱밥과 미강으로 혼합한 배지에 곰보버섯균을 접종하여 배양하는 단계(단계 1);In addition, the present invention includes the steps of inoculating and culturing morel mushroom bacteria in a medium mixed with sawdust and rice bran (step 1);
상기 단계 1의 배양된 곰보버섯균을 곰보버섯 재배용 배지에 접종하는 단계(단계 2); 및Inoculating the cultured morel mushroom bacteria of step 1 into a morel mushroom cultivation medium (step 2); and
상기 접종된 곰보버섯균을 20 내지 25℃의 배양온도, 60 내지 70%의 상대습도 조건에서 25 내지 30일간 배양하여 곰보버섯균으로부터 균핵을 형성하는 단계(단계 3); 를 포함하되,Culturing the inoculated morel mushroom bacteria for 25 to 30 days at a culture temperature of 20 to 25°C and relative humidity of 60 to 70% to form sclerotia from the morel mushroom bacteria (step 3); Including,
상기 단계 2의 곰보버섯 재배용 배지는 참나무 발효톱밥, 부엽토, 및 밀, 왕겨 및 옥수수가루로 이루어진 군으로부터 선택되는 하나 이상을 포함하고,The medium for growing morel mushrooms in step 2 includes at least one selected from the group consisting of fermented oak sawdust, humus soil, and wheat, rice husk, and corn flour,
상기 단계 2의 곰보버섯 재배용 배지는 생석회, 석고, KH2PO4 및 백설탕을 추가로 포함하는 것을 특징으로 하는 곰보버섯 균사체의 재배방법을 제공한다.The medium for growing morel mushrooms in step 2 provides a method of cultivating morel mushroom mycelium, characterized in that it additionally contains quicklime, gypsum, KH 2 PO 4 and white sugar.
나아가 본 발명은 상기 재배방법에 의해 재배된 곰보버섯 균사체를 제공한다.Furthermore, the present invention provides morel mushroom mycelium cultivated by the above cultivation method.
또한, 본 발명은 톱밥과 미강으로 혼합한 배지에 곰보버섯균을 접종하여 배양하는 단계(단계 1);In addition, the present invention includes the steps of inoculating and culturing morel mushroom bacteria in a medium mixed with sawdust and rice bran (step 1);
상기 단계 1의 배양된 곰보버섯균을 곰보버섯 재배용 배지에 접종하는 단계(단계 2); Inoculating the cultured morel mushroom bacteria of step 1 into a morel mushroom cultivation medium (step 2);
상기 접종된 곰보버섯균을 20 내지 25℃의 배양온도, 60 내지 70%의 상대습도 조건에서 25 내지 30일간 배양하여 곰보버섯균으로부터 균핵을 형성하는 단계(단계 3); 및Culturing the inoculated morel mushroom bacteria for 25 to 30 days at a culture temperature of 20 to 25°C and relative humidity of 60 to 70% to form sclerotia from the morel mushroom bacteria (step 3); and
상기 형성된 균핵으로부터 곰보버섯 자실체를 생육하는 단계(단계 4); 를 포함하되,Growing morel mushroom fruiting bodies from the formed sclerotia (step 4); Including,
상기 단계 2의 곰보버섯 재배용 배지는 참나무 발효톱밥, 부엽토, 및 밀, 왕겨 및 옥수수가루로 이루어진 군으로부터 선택되는 하나 이상을 포함하고,The medium for growing morel mushrooms in step 2 includes at least one selected from the group consisting of fermented oak sawdust, humus soil, and wheat, rice husk, and corn flour,
상기 단계 2의 곰보버섯 재배용 배지는 생석회, 석고, KH2PO4 및 백설탕을 추가로 포함하는 것을 특징으로 하는 곰보버섯 자실체의 재배방법을 제공한다.The medium for growing morel mushrooms in step 2 provides a method of cultivating morel mushroom fruiting bodies, characterized in that it additionally contains quicklime, gypsum, KH 2 PO 4 and white sugar.
나아가 본 발명은 상기 재배방법에 의해 재배된 곰보버섯 자실체를 제공한다.Furthermore, the present invention provides morel mushroom fruiting bodies cultivated by the above cultivation method.
본 발명은 곰보버섯의 재배용 배지 조성물 및 곰보버섯의 재배방법에 관한 것으로서, 본 발명의 배지 조성물을 이용하여 배양할 경우, 자실체 배양이 어려웠던 곰보버섯의 자실체를 배양할 수 있으며, 또한, 본 발명의 배지 조성물을 이용할 경우, 노지재배, 하우스재배 또는 상자재배 등 재배방법에 관계없이 곰보버섯의 생장 효율이 우수하여 곰보버섯을 저비용으로 대량생산이 가능하고, 생산비 절감 효과가 있다.The present invention relates to a culture medium composition for the cultivation of morel mushrooms and a method for cultivating morel mushrooms. When cultured using the medium composition of the present invention, the fruiting bodies of morel mushrooms, which were difficult to cultivate, can be cultivated. In addition, the present invention When using a medium composition, the growth efficiency of morel mushrooms is excellent regardless of the cultivation method such as open field cultivation, house cultivation, or box cultivation, making it possible to mass produce morel mushrooms at low cost and reducing production costs.
도 1은 (a)폴리프로필렌병 또는 (b)비닐봉지에서 곰보버섯 종균배양을 나타낸 이미지이다.
도 2는 배지혼합비에 따른 곰보버섯균의 균사생육을 나타낸 이미지이다.
도 3은 곰보버섯 재배시 자실체 형성 및 생육과정을 나타낸 이미지이다.
도 4는 곰보버섯 상자재배시 자실체 형성 및 생육과정을 나타낸 이미지이다.
도 5는 곰보버섯 하우스재배시 자실체 형성 및 생육과정을 나타낸 이미지이다.
도 6은 본 발명의 곰보버섯 재배용 배지에서 수확한 곰보버섯 자실체를 나타낸 이미지이다.
도 7은 수확한 곰보버섯 자실체의 특성을 조사한 것이다.Figure 1 is an image showing morel mushroom spawn culture in (a) a polypropylene bottle or (b) a plastic bag.
Figure 2 is an image showing mycelial growth of morel mushroom fungi according to medium mixing ratio.
Figure 3 is an image showing the fruiting body formation and growth process during morel mushroom cultivation.
Figure 4 is an image showing the fruiting body formation and growth process during box cultivation of morel mushrooms.
Figure 5 is an image showing the fruiting body formation and growth process during house cultivation of morel mushrooms.
Figure 6 is an image showing morel mushroom fruiting bodies harvested from the morel mushroom cultivation medium of the present invention.
Figure 7 examines the characteristics of harvested morel mushroom fruiting bodies.
이하, 본 발명을 보다 상세히 설명한다.Hereinafter, the present invention will be described in more detail.
곰보버섯 재배용 배지 조성물Media composition for morel mushroom cultivation
본 발명은 참나무 발효톱밥; 부엽토; 및 밀, 왕겨 및 옥수수가루로 이루어진 군으로부터 선택되는 하나 이상;을 포함하는 것을 특징으로 하는 곰보버섯 재배용 배지 조성물을 제공한다.The present invention relates to fermented oak sawdust; Humus; and at least one selected from the group consisting of wheat, rice husk, and cornmeal.
본 발명의 조성물에 있어서, 상기 조성물은 생석회, 석고, KH2PO4 및 백설탕을 추가로 포함할 수 있고, 상기 곰보버섯 재배용 배지 조성물 100 중량부 기준 생석회 1 내지 2 중량부, 석고 1.5 내지 2.5 중량부, KH2PO4 0.005 내지 0.015 중량부 및 백설탕 2.5 내지 7.5 중량부 포함할 수 있다.In the composition of the present invention, the composition may further include quicklime, gypsum, KH 2 PO 4 and white sugar, and 1 to 2 parts by weight of quicklime and 1.5 to 2.5 parts by weight of gypsum based on 100 parts by weight of the medium composition for morel mushroom cultivation. parts, 0.005 to 0.015 parts by weight of KH 2 PO 4 and 2.5 to 7.5 parts by weight of white sugar.
본 발명의 조성물에 있어서, 상기 참나무 발효톱밥은 참나무 생톱밥에 장시간 수분을 첨가하고 뒤집기를 하면서 야외 발효시킨 발효물로 수지 및 유해성분을 유출시키고, 부숙시킨 후 보습력을 높여 버섯균이 이용하기 용이한 상태로 만든 톱밥이다.In the composition of the present invention, the oak fermented sawdust is a fermented product that is fermented outdoors by adding moisture to raw oak sawdust and turning it over for a long time, leaking out resin and harmful components, and increasing the moisturizing power after composting, making it easy for mushrooms to use. It is sawdust made into a state.
본 발명의 일실시예에 있어서, 상기 조성물은 참나무 발효톱밥 100 중량부 기준 부엽토 9 내지 48 중량부 및 밀 23 내지 63 중량부 포함할 수 있고, 바람직하게는 참나무 발효톱밥 100 중량부 기준 부엽토 38 내지 48 중량부 및 밀 52 내지 63 중량부를 포함하거나, 참나무 발효톱밥 100 중량부 기준 부엽토 9 내지 20 중량부 및 밀 23 내지 34 중량부 포함할 수 있다.In one embodiment of the present invention, the composition may include 9 to 48 parts by weight of humus and 23 to 63 parts by weight of wheat based on 100 parts by weight of oak fermented sawdust, and preferably 38 to 63 parts by weight of humus based on 100 parts by weight of oak fermented sawdust. It may contain 48 parts by weight and 52 to 63 parts by weight of wheat, or it may contain 9 to 20 parts by weight of humus and 23 to 34 parts by weight of wheat based on 100 parts by weight of fermented oak sawdust.
본 발명의 일실시예에 있어서, 상기 조성물은 참나무 발효톱밥 100 중량부 기준 부엽토 38 내지 48 중량부 및 옥수수가루 52 내지 62 중량부 포함할 수 있다.In one embodiment of the present invention, the composition may include 38 to 48 parts by weight of humus and 52 to 62 parts by weight of corn flour based on 100 parts by weight of fermented oak sawdust.
본 발명의 일실시예에 있어서, 상기 조성물은 참나무 발효톱밥 100 중량부 기준 부엽토 28 내지 40 중량부, 밀 95 내지 105 중량부 및 왕겨 95 내지 105 중량부 포함할 수 있다.In one embodiment of the present invention, the composition may include 28 to 40 parts by weight of humus, 95 to 105 parts by weight of wheat, and 95 to 105 parts by weight of rice husk based on 100 parts by weight of fermented oak sawdust.
본 발명의 일실시예에 있어서, 상기 조성물은 참나무 발효톱밥 100 중량부 기준 부엽토 35 내지 50 중량부, 밀 20 내지 40 중량부 및 옥수수가루 20 내지 40 중량부 포함할 수 있다.In one embodiment of the present invention, the composition may include 35 to 50 parts by weight of humus, 20 to 40 parts by weight of wheat, and 20 to 40 parts by weight of corn flour based on 100 parts by weight of fermented oak sawdust.
곰보버섯 균사체의 재배방법How to cultivate morel mushroom mycelium
본 발명은 톱밥과 미강으로 혼합한 배지에 곰보버섯균을 접종하여 배양하는 단계(단계 1);The present invention includes the steps of inoculating and culturing morel mushroom bacteria in a medium mixed with sawdust and rice bran (step 1);
상기 단계 1의 배양된 곰보버섯균을 곰보버섯 재배용 배지에 접종하는 단계(단계 2); 및Inoculating the cultured morel mushroom bacteria of step 1 into a morel mushroom cultivation medium (step 2); and
상기 접종된 곰보버섯균을 20 내지 25℃의 배양온도, 60 내지 70%의 상대습도 조건에서 25 내지 30일간 배양하여 곰보버섯균으로부터 균핵을 형성하는 단계(단계 3); 를 포함하되,Culturing the inoculated morel mushroom bacteria for 25 to 30 days at a culture temperature of 20 to 25°C and relative humidity of 60 to 70% to form sclerotia from the morel mushroom bacteria (step 3); Including,
상기 단계 2의 곰보버섯 재배용 배지는 참나무 발효톱밥, 부엽토, 및 밀, 왕겨 및 옥수수가루로 이루어진 군으로부터 선택되는 하나 이상을 포함하고,The medium for growing morel mushrooms in step 2 includes at least one selected from the group consisting of fermented oak sawdust, humus soil, and wheat, rice husk, and corn flour,
상기 단계 2의 곰보버섯 재배용 배지는 생석회, 석고, KH2PO4 및 백설탕을 추가로 포함하는 것을 특징으로 하는 곰보버섯 균사체의 재배방법을 제공한다.The medium for growing morel mushrooms in step 2 provides a method of cultivating morel mushroom mycelium, characterized in that it additionally contains quicklime, gypsum, KH 2 PO 4 and white sugar.
본 발명의 재배방법에 있어서, 상기 참나무 발효톱밥은 참나무 생톱밥에 장시간 수분을 첨가하고 뒤집기를 하면서 야외 발효시킨 발효물로 수지 및 유해성분을 유출시키고, 부숙시킨 후 보습력을 높여 버섯균이 이용하기 용이한 상태로 만든 톱밥이다.In the cultivation method of the present invention, the fermented oak sawdust is a fermented product that is fermented outdoors by adding moisture to fresh oak sawdust for a long time and turning it over, leaking out resin and harmful components, and increasing the moisturizing power after composting, making it easy for mushrooms to use. It is sawdust made in one state.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지 100 중량부 기준 생석회 1 내지 2 중량부, 석고 1.5 내지 2.5 중량부, KH2PO4 0.005 내지 0.015 중량부 및 백설탕 2.5 내지 7.5 중량부 포함할 수 있다.In the cultivation method of the present invention, based on 100 parts by weight of the morel mushroom cultivation medium, it may include 1 to 2 parts by weight of quicklime, 1.5 to 2.5 parts by weight of gypsum, 0.005 to 0.015 parts by weight of KH 2 PO 4 and 2.5 to 7.5 parts by weight of white sugar. there is.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지는 참나무 발효톱밥 100 중량부 기준 부엽토 9 내지 48 중량부 및 밀 23 내지 63 중량부 포함할 수 있고, 바람직하게는 참나무 발효톱밥 100 중량부 기준 부엽토 38 내지 48 중량부 및 밀 52 내지 63 중량부를 포함하거나, 참나무 발효톱밥 100 중량부 기준 부엽토 9 내지 20 중량부 및 밀 23 내지 34 중량부 포함할 수 있다.In the cultivation method of the present invention, the medium for morel mushroom cultivation may include 9 to 48 parts by weight of humus based on 100 parts by weight of oak fermented sawdust and 23 to 63 parts by weight of wheat, and preferably contains humus based on 100 parts by weight of oak fermented sawdust. It may contain 38 to 48 parts by weight and 52 to 63 parts by weight of wheat, or it may contain 9 to 20 parts by weight of humus and 23 to 34 parts by weight of wheat based on 100 parts by weight of fermented oak sawdust.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지는 참나무 발효톱밥 100 중량부 기준 부엽토 38 내지 48 중량부 및 옥수수가루 52 내지 62 중량부 포함할 수 있다.In the cultivation method of the present invention, the medium for morel mushroom cultivation may include 38 to 48 parts by weight of humus and 52 to 62 parts by weight of corn flour based on 100 parts by weight of fermented oak sawdust.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지는 참나무 발효톱밥 100 중량부 기준 부엽토 28 내지 40 중량부, 밀 95 내지 105 중량부 및 왕겨 95 내지 105 중량부 포함할 수 있다.In the cultivation method of the present invention, the medium for morel mushroom cultivation may include 28 to 40 parts by weight of humus, 95 to 105 parts by weight of wheat, and 95 to 105 parts by weight of rice husk based on 100 parts by weight of fermented oak sawdust.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지는 참나무 발효톱밥 100 중량부 기준 부엽토 35 내지 50 중량부, 밀 20 내지 40 중량부 및 옥수수가루 20 내지 40 중량부 포함할 수 있다.In the cultivation method of the present invention, the medium for morel mushroom cultivation may include 35 to 50 parts by weight of humus, 20 to 40 parts by weight of wheat, and 20 to 40 parts by weight of corn flour based on 100 parts by weight of fermented oak sawdust.
또한, 본 발명은 상기 곰보버섯 균사체의 재배방법에 의해 재배된 곰보버섯 균사체를 제공한다.In addition, the present invention provides morel mushroom mycelium cultivated by the above morel mushroom mycelium cultivation method.
곰보버섯 자실체의 재배방법Cultivation method of morel mushroom fruiting body
본 발명은 톱밥과 미강으로 혼합한 배지에 곰보버섯균을 접종하여 배양하는 단계(단계 1);The present invention includes the steps of inoculating and culturing morel mushroom bacteria in a medium mixed with sawdust and rice bran (step 1);
상기 단계 1의 배양된 곰보버섯균을 곰보버섯 재배용 배지에 접종하는 단계(단계 2);Inoculating the cultured morel mushroom bacteria of step 1 into a morel mushroom cultivation medium (step 2);
상기 접종된 곰보버섯균을 20 내지 25℃의 배양온도, 60 내지 70%의 상대습도 조건에서 25 내지 30일간 배양하여 곰보버섯균으로부터 균핵을 형성하는 단계(단계 3); 및Culturing the inoculated morel mushroom bacteria for 25 to 30 days at a culture temperature of 20 to 25°C and relative humidity of 60 to 70% to form sclerotia from the morel mushroom bacteria (step 3); and
상기 형성된 균핵으로부터 곰보버섯 자실체를 생육하는 단계(단계 4); 를 포함하되,Growing morel mushroom fruiting bodies from the formed sclerotia (step 4); Including,
상기 단계 2의 곰보버섯 재배용 배지는 참나무 발효톱밥, 부엽토, 및 밀, 왕겨 및 옥수수가루로 이루어진 군으로부터 선택되는 하나 이상을 포함하고,The medium for growing morel mushrooms in step 2 includes at least one selected from the group consisting of fermented oak sawdust, humus soil, and wheat, rice husk, and corn flour,
상기 단계 2의 곰보버섯 재배용 배지는 생석회, 석고, KH2PO4 및 백설탕을 추가로 포함하는 것을 특징으로 하는 곰보버섯 자실체의 재배방법을 제공한다.The medium for growing morel mushrooms in step 2 provides a method of cultivating morel mushroom fruiting bodies, characterized in that it additionally contains quicklime, gypsum, KH 2 PO 4 and white sugar.
본 발명의 재배방법에 있어서, 상기 참나무 발효톱밥은 참나무 생톱밥에 장시간 수분을 첨가하고 뒤집기를 하면서 야외 발효시킨 발효물로 수지 및 유해성분을 유출시키고, 부숙시킨 후 보습력을 높여 버섯균이 이용하기 용이한 상태로 만든 톱밥이다.In the cultivation method of the present invention, the fermented oak sawdust is a fermented product that is fermented outdoors by adding moisture to fresh oak sawdust for a long time and turning it over, leaking out resin and harmful components, and increasing the moisturizing power after composting, making it easy for mushrooms to use. It is sawdust made in one state.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지 100 중량부 기준 생석회 1 내지 2 중량부, 석고 1.5 내지 2.5 중량부, KH2PO4 0.005 내지 0.015 중량부 및 백설탕 2.5 내지 7.5 중량부 포함할 수 있다.In the cultivation method of the present invention, based on 100 parts by weight of the morel mushroom cultivation medium, it may include 1 to 2 parts by weight of quicklime, 1.5 to 2.5 parts by weight of gypsum, 0.005 to 0.015 parts by weight of KH 2 PO 4 and 2.5 to 7.5 parts by weight of white sugar. there is.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지는 참나무 발효톱밥 100 중량부 기준 부엽토 9 내지 48 중량부 및 밀 23 내지 63 중량부 포함할 수 있고, 바람직하게는 참나무 발효톱밥 100 중량부 기준 부엽토 38 내지 48 중량부 및 밀 52 내지 63 중량부를 포함하거나, 참나무 발효톱밥 100 중량부 기준 부엽토 9 내지 20 중량부 및 밀 23 내지 34 중량부 포함할 수 있다.In the cultivation method of the present invention, the medium for morel mushroom cultivation may include 9 to 48 parts by weight of humus based on 100 parts by weight of oak fermented sawdust and 23 to 63 parts by weight of wheat, and preferably contains humus based on 100 parts by weight of oak fermented sawdust. It may contain 38 to 48 parts by weight and 52 to 63 parts by weight of wheat, or it may contain 9 to 20 parts by weight of humus and 23 to 34 parts by weight of wheat based on 100 parts by weight of fermented oak sawdust.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지는 참나무 발효톱밥 100 중량부 기준 부엽토 38 내지 48 중량부 및 옥수수가루 52 내지 62 중량부 포함할 수 있다.In the cultivation method of the present invention, the medium for morel mushroom cultivation may include 38 to 48 parts by weight of humus and 52 to 62 parts by weight of corn flour based on 100 parts by weight of fermented oak sawdust.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지는 참나무 발효톱밥 100 중량부 기준 부엽토 28 내지 40 중량부, 밀 95 내지 105 중량부 및 왕겨 95 내지 105 중량부 포함할 수 있다.In the cultivation method of the present invention, the medium for morel mushroom cultivation may include 28 to 40 parts by weight of humus, 95 to 105 parts by weight of wheat, and 95 to 105 parts by weight of rice husk based on 100 parts by weight of fermented oak sawdust.
본 발명의 재배방법에 있어서, 상기 곰보버섯 재배용 배지는 참나무 발효톱밥 100 중량부 기준 부엽토 35 내지 50 중량부, 밀 20 내지 40 중량부 및 옥수수가루 20 내지 40 중량부 포함할 수 있다.In the cultivation method of the present invention, the medium for morel mushroom cultivation may include 35 to 50 parts by weight of humus, 20 to 40 parts by weight of wheat, and 20 to 40 parts by weight of corn flour based on 100 parts by weight of fermented oak sawdust.
본 발명의 재배방법에 있어서, 상기 단계 4는 상기 단계 3에서 형성된 균핵을 상자재배, 노지재배 또는 하우스 재배하여 균핵으로부터 곰보버섯 자실체를 생육할 수 있다.In the cultivation method of the present invention, in step 4, morel mushroom fruiting bodies can be grown from the sclerotia by box cultivation, open field cultivation, or house cultivation of the sclerotia formed in step 3.
상기 상자재배의 경우, 토양을 상자에 일정량 넣고 상기 단계 3의 균핵을 뿌려 균사체를 생육시킨 뒤, 토양표면에 균사체 생육이 만연하게 되면 생석회, 석고, KH2PO4 또는 백설탕과 같은 영양원을 처리하고 10 내지 20℃의 온도, 60 내지 95% 상대습도 및 800 내지 1000ppm 이산화탄소 농도로 조절하여 곰보버섯 자실체를 재배할 수 있고, 상기 노지재배 또는 하우스재배의 경우, 상기 단계 3의 균핵을 뿌려 균사체를 생육시킨 뒤, 토양표면에 균사체 생육이 만연하게 되면 생석회, 석고, KH2PO4 또는 백설탕과 같은 영양원을 처리하고 5 내지 20℃의 온도 및 80 내지 90% 상대습도로 조절하여 곰보버섯 자실체를 재배할 수 있다.In the case of box cultivation, a certain amount of soil is placed in the box and the sclerotia of step 3 are sprinkled to grow mycelium. When mycelium growth becomes widespread on the soil surface, a nutrient source such as quicklime, gypsum, KH 2 PO 4 or white sugar is treated. Morel mushroom fruiting bodies can be grown by adjusting the temperature to 10 to 20°C, relative humidity to 60 to 95%, and carbon dioxide concentration to 800 to 1000 ppm. In the case of outdoor cultivation or house cultivation, the mycelium is grown by spraying the sclerotia of step 3. After this, when mycelium growth becomes rampant on the soil surface, nutrient sources such as quicklime, gypsum, KH 2 PO 4 or white sugar are treated and the temperature is adjusted to 5 to 20 ℃ and 80 to 90% relative humidity to cultivate morel mushroom fruiting bodies. You can.
또한, 본 발명은 상기 곰보버섯 자실체의 재배방법에 의해 재배된 곰보버섯 자실체를 제공한다.In addition, the present invention provides morel mushroom fruiting bodies cultivated by the above-mentioned morel mushroom fruiting body cultivation method.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited to the following examples.
<준비예 1> 시험균주 및 종균의 준비<Preparation Example 1> Preparation of test strains and spawn
시험에 사용한 균주는 국립원예특작과학원 버섯과에 보관하고 있는 곰보버섯(Morchella sp.)을 시험균주로 사용하였다. The strain used in the test was Morchella sp., stored in the Mushroom Department of the National Institute of Horticultural and Herbal Science.
<준비예 2> 접종원 배양<Preparation Example 2> Inoculum culture
상기 준비예 1의 균주를 PDA(potato dextrose agar) 평판배지에서 7일간 배양 후, 미송톱밥과 미강혼합배지(80:20, v/v)를 250ml 삼각플라스크에 100ml 가량을 담아 121℃에서 40분간 살균한 배지에 곰보버섯균을 접종하여 20일간 배양한 후 접종원으로 사용하였다.After cultivating the strain of Preparation Example 1 for 7 days on PDA (potato dextrose agar) plate medium, approximately 100 ml of Douglas fir sawdust and rice bran mixed medium (80:20, v/v) was placed in a 250 ml Erlenmeyer flask and incubated at 121°C for 40 minutes. Morel mushroom bacteria were inoculated into the sterilized medium, cultured for 20 days, and then used as an inoculum.
<준비예 3> 곰보버섯 재배용 배지의 준비<Preparation Example 3> Preparation of medium for morel mushroom cultivation
하기 표 1과 같은 조성으로 곰보버섯 재배용 배지를 준비하였다. A medium for morel mushroom cultivation was prepared with the composition shown in Table 1 below.
<실시예 1> 곰보버섯 종균 배양<Example 1> Morel mushroom spawn culture
<1-1><1-1>
상기 준비예 3-1의 배지를 수분함량을 65% 내외로 조절하였고, PP(Polyproylene)병과 비닐봉지에 목까지 동일한 압력으로 배지를 담아서 121℃에서 90분간 고압살균 후 20℃ 내외로 냉각하고 준비된 접종원을 접종하였다(도 1 참조). 접종된 배지는 배양 온도 20∼25℃, 상대습도 60∼70%로 조절된 배양실에서 25∼30일간 배양하였다. 배양기간 중에는 혼합배지별 잡균의 오염여부를 조사하여 오염된 배지를 즉시 제거하였다. The moisture content of the medium in Preparation Example 3-1 was adjusted to around 65%, and the medium was placed in a PP (Polyproylene) bottle and a plastic bag at the same pressure up to the neck, sterilized under high pressure at 121°C for 90 minutes, cooled to around 20°C, and prepared. The inoculum was inoculated (see Figure 1). The inoculated medium was cultured for 25 to 30 days in a culture room controlled at a culture temperature of 20 to 25°C and a relative humidity of 60 to 70%. During the culture period, each mixed medium was examined for contamination with various bacteria, and the contaminated medium was immediately removed.
<1-2 내지 1-13><1-2 to 1-13>
상기 준비예 3-2 내지 3-13의 배지를 사용한 것을 제외하고는 상기 실시예 1-1과 동일한 조건에서 곰보버섯 종균 배양하였다.Morel mushroom spawn was cultured under the same conditions as in Example 1-1, except that the media of Preparation Examples 3-2 to 3-13 were used.
<실시예 2> 곰보버섯 종균 접종 및 곰보버섯 자실체 생육관리<Example 2> Morel mushroom spawn inoculation and morel mushroom fruiting body growth management
상자재배는 온도 및 습도조절이 가능하고 이산화탄소 배출을 위한 환풍시설을 구비하고 있는 재배시설을 이용하여 토양을 상자에 일정량을 넣고 배양이 종료된 실시예 1-7 내지 1-9의 종균을 뿌리고 토양으로 복토를 하여 곰보버섯 균사를 생육시키는 단계 후 토양표면이 균사로 만연하게 되면 영양원을 처리하여 온도와 습도를 유지하면서 버섯 발생작업을 한다. 곰보버섯 재배시설의 환경은 온도 10∼20℃, 상대습도 60∼95%, CO2농도 1000ppm이하로 관리하여 곰보버섯 자실체의 생육을 유도하였다.Box cultivation uses a cultivation facility that is capable of controlling temperature and humidity and is equipped with ventilation facilities for carbon dioxide emissions. A certain amount of soil is placed in a box, and the seed germs of Examples 1-7 to 1-9 that have been cultured are sprinkled and the soil is grown. After the step of growing morel mushroom mycelium by covering the soil, when the soil surface is infested with mycelium, the nutrient source is treated and mushroom growth is performed while maintaining temperature and humidity. The environment of the morel mushroom cultivation facility was managed at a temperature of 10 to 20°C, relative humidity of 60 to 95%, and CO 2 concentration of less than 1000 ppm to induce the growth of morel mushroom fruiting bodies.
하우스재배는 일반 노지에 비닐 및 차광망을 씌어 재배하는 방법과 기존의 비닐하우스를 사용하는 방법이 있으며, 일반 비닐하우스에 측창의 개방이 가능하고 차광망을 씌워 직사광선이 비치지 않는 시설에서 재배가 가능하다. 노지 및 하우스재배는 재배 토양을 트랙터로 일정한 깊이로 갈고난 후 실시예 1-7 내지 1-9의 종균을 뿌리고 일정기간 배양을 거쳐 토양표면에 곰보버섯 균사가 만연하면 영양원을 처리하고 토양 온도와 습도를 유지하면서 버섯 발생작업을 한다. 곰보버섯 재배시설의 환경은 생육온도를 5∼20℃로 관리하면서 버섯 발생시에는 상대습도를 80∼90%을 유지하면서 곰보버섯 자실체의 생육을 유도하였다(도 3 및 도 6 참조).House cultivation includes a method of growing in a general open field covered with plastic and a shading net, and a method of using an existing greenhouse. In a general greenhouse, the side windows can be opened and a shading net can be placed to allow cultivation in a facility that does not receive direct sunlight. For open field and house cultivation, the cultivation soil is plowed to a certain depth with a tractor, the spawn of Examples 1-7 to 1-9 is sprayed, and after culturing for a certain period of time, when morel mushroom mycelium is prevalent on the soil surface, the nutrient source is treated, and the soil temperature and Mushroom development is carried out while maintaining humidity. The environment of the morel mushroom cultivation facility induced the growth of morel mushroom fruiting bodies by managing the growth temperature at 5 to 20°C and maintaining the relative humidity at 80 to 90% when mushrooms were generated (see Figures 3 and 6).
곰보버섯 수확은 버섯 발생 후 7∼10일이 되면 자실체가 성숙하게 되어 수확이 가능하고 수확후 바로 건조작업을 하는 것이 바람직하며, 건조온도는 45∼50℃에서 4∼5시간 건조하는 것이 바람직하다.Morel mushrooms can be harvested 7 to 10 days after the mushrooms emerge as the fruiting bodies mature and can be harvested. It is advisable to dry them immediately after harvesting. The drying temperature is preferably 45 to 50 degrees Celsius for 4 to 5 hours. .
<실험예 1> 배지혼합비율에 따른 균사 생장량 및 균사밀도 측정<Experimental Example 1> Measurement of mycelial growth and mycelial density according to medium mixing ratio
상기 실시예 1-1 내지 1-13의 배지혼합비율에 따른 균사 생장량 및 균사밀도를 도 2의 컬럼을 이용하여 생장시켜 조사하였고, 시험관은 20 mm×200 mm를 사용하였으며, 각각의 혼합배지를 충진하여 고압살균하고 종균 접종 후 25℃ 항온실에서 배양시키면서 일정한 간격으로 균사 생장길이를 측정하여 균사 생장량으로 조사하였다.The mycelial growth amount and mycelial density according to the medium mixing ratio of Examples 1-1 to 1-13 were investigated by growing using the column of Figure 2, a test tube of 20 mm × 200 mm was used, and each mixed medium was It was filled and sterilized under high pressure, and after seed inoculation, it was cultured in a constant temperature room at 25°C and the mycelial growth length was measured at regular intervals to determine the amount of mycelial growth.
그 결과, 표 2 및 도 2에 나타낸 바와 같이, 균사 생장량은 배양 11일 후 실시예 1-7의 배지와 실시예 1-9의 배지의 혼합비율에서 150mm로 균사생장이 가장 빨랐다. 배지혼합비율에 따른 균사밀도는 실시예 1-4와 실시예 1-5의 배지에서 가장 낮았고, 균사생장이 빨랐던 실시예 1-7과 실시예 1-9의 배지에서는 높은 균사밀도를 보였다. 균핵형성 정도는 실시예 1-7과 실시예 1-8의 배지에서 가장 높았고, 실시예 1-9, 실시예 1-12 및 실시예 1-13의 배지에서도 중간정도의 균핵형성을 보이는 것을 확인하였다.As a result, as shown in Table 2 and Figure 2, mycelial growth was the fastest at 150 mm at a mixing ratio of the medium of Example 1-7 and the medium of Example 1-9 after 11 days of culture. The mycelium density according to the medium mixing ratio was lowest in the media of Examples 1-4 and 1-5, and the media of Examples 1-7 and Example 1-9, where mycelial growth was fast, showed high mycelial density. The degree of sclerotia formation was highest in the media of Examples 1-7 and Examples 1-8, and it was confirmed that the media of Examples 1-9, Examples 1-12, and Examples 1-13 also showed a moderate degree of sclerotia formation. did.
(mm)/11일Mycelial growth
(mm)/11 days
밀도*spawn
density*
형성*sclerotia
formation*
*균사밀도 및 균핵형성 : +, 약; ++, 중; +++, 강*Hyphal density and sclerotia formation: +, approximately; ++, medium; +++, river
상기 결과로 인해 본 발명의 배지 조성에서 배양된 곰보버섯의 균사생육, 균사밀도 및 균핵형성이 우수한 것을 확인하였다.As a result of the above results, it was confirmed that the mycelial growth, mycelial density, and sclerotia formation of morel mushrooms cultured in the medium composition of the present invention were excellent.
<실험예 2> 곰보버섯 생육 및 자실체 특성 확인<Experimental Example 2> Confirmation of morel mushroom growth and fruiting body characteristics
상자재배(도 4, 표 3)에서 곰보버섯 재배 결과 자실체의 평균 무게는 33.2g이였고, 길이는 133.9mm, 갓길이는 73mm, 대길이는 52.2mm였고, 노지 및 하우스재배(도 5, 표 4)에서는 평균 무게는 32.2g이였고, 길이는 132.7mm, 갓길이는 63.7mm, 대길이는 57.0mm인 것을 확인하였다.As a result of morel mushroom cultivation in box cultivation (Figure 4, Table 3), the average weight of the fruiting body was 33.2g, the length was 133.9mm, the shoulder length was 73mm, and the length was 52.2mm, and the field and house cultivation (Figure 5, Table 3) In 4), the average weight was 32.2g, the length was 132.7mm, the shoulder length was 63.7mm, and the main length was 57.0mm.
또한, 자실체의 육안적 특성은 형태가 갓은 뾰족한 타원형, 색깔은 갈색 및 황갈색이며 윗부분은 원형이며 짙은 갈색을 띄었으며, 대는 표면이 긁고 큰주름이 있으며 매끄럽고 색깔은 담황백색인 것을 확인하였다(표 5, 도7).In addition, the visual characteristics of the fruiting body showed that the cap was oval with a pointed shape, the color was brown and yellow-brown, the upper part was round and dark brown, and the stem was smooth with scratches and large wrinkles on the surface, and the color was pale yellow-white (Table 5, Figure 7).
따라서, 본 발명에 따른 곰보버섯 재배용 배지를 사용할 경우, 노지재배, 하우스재배 또는 상자재배 등 재배방법에 관계없이 곰보버섯 자실체의 생장 효율이 우수한 것을 확인하였다.Therefore, it was confirmed that when using the morel mushroom cultivation medium according to the present invention, the growth efficiency of morel mushroom fruiting bodies was excellent regardless of the cultivation method such as open field cultivation, house cultivation, or box cultivation.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특히 청구범위에 나타나 있으며, 그와 동등한 범위내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.So far, the present invention has been examined focusing on its preferred embodiments. A person skilled in the art to which the present invention pertains will understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a restrictive perspective. The scope of the present invention is set forth in particular in the claims rather than the foregoing description, and all differences within the equivalent scope should be construed as being included in the present invention.
Claims (2)
상기 단계 1의 배양된 곰보버섯균을 곰보버섯 자실체 인공재배용 배지 조성물에 접종하는 단계(단계 2);
상기 접종된 곰보버섯균을 20 내지 25℃의 배양온도, 60 내지 70%의 상대습도 조건에서 25 내지 30일간 배양하여 곰보버섯균으로부터 균핵을 형성하는 단계(단계 3); 및
상기 형성된 균핵을 상자 재배 및 하우스 재배 중 어느 하나의 버섯 재배 환경 조건의 토양에 접종한 후, 토양 표면에 균사체 생육이 만연하게 되면 영양원을 처리하고, 곰보버섯 자실체를 생육하는 단계(단계 4); 를 포함하고,
상기 단계 2의 곰보버섯 자실체 인공재배용 배지 조성물은 참나무 발효톱밥 100 중량부 기준, 부엽토 40.85-45.15 중량부, 밀 54.15-59.85 중량부, 생석회 2.85-3.15 중량부, 석고 3.8-4.2 중량부, KH2PO4 0.019-0.21 중량부 및 백설탕 9.5-10.5 중량부를 포함하는 것을 특징으로 하고,
상기 단계 4의 상자 재배의 경우 영양원을 처리하고 10 내지 20℃의 온도, 60 내지 95% 상대습도 및 800 내지 1000ppm 이산화탄소 농도로 조절하여 곰보버섯 자실체를 생육하는 것을 특징으로 하고,
상기 단계 4의 하우스 재배의 경우 영양원을 처리하고 5 내지 20℃의 온도 및 80 내지 90% 상대습도로 조절하여 곰보버섯 자실체를 생육하는 것을 특징으로 하며,
상기 단계 4의 영양원은 생석회 1.5 중량부 기준, 석고 1.5-2.5 중량부, KH2PO4 0.005-0.015 중량부 및 백설탕 2.5-7.5 중량부를 포함하는 것을 특징으로 하는,
곰보버섯 자실체의 재배방법.
Inoculating and culturing morel mushroom bacteria in a medium mixed with sawdust and rice bran (step 1);
Inoculating the cultured morel mushroom bacteria of step 1 into a medium composition for artificial cultivation of morel mushroom fruiting bodies (step 2);
Culturing the inoculated morel mushroom bacteria for 25 to 30 days at a culture temperature of 20 to 25°C and relative humidity of 60 to 70% to form sclerotia from the morel mushroom bacteria (step 3); and
Inoculating the formed sclerotia into soil under either mushroom cultivation environmental conditions of box cultivation or house cultivation, then processing the nutrient source when mycelium growth becomes rampant on the soil surface, and growing morel mushroom fruiting bodies (step 4); Including,
The medium composition for artificial cultivation of morel mushroom fruiting bodies in step 2 is based on 100 parts by weight of fermented oak sawdust, 40.85-45.15 parts by weight of humus, 54.15-59.85 parts by weight of wheat, 2.85-3.15 parts by weight of quicklime, 3.8-4.2 parts by weight of gypsum, and KH 2. Characterized in that it contains 0.019-0.21 parts by weight of PO 4 and 9.5-10.5 parts by weight of white sugar,
In the case of box cultivation in step 4, the nutrient source is treated and the morel mushroom fruiting body is grown by adjusting the temperature to 10 to 20°C, 60 to 95% relative humidity, and 800 to 1000 ppm carbon dioxide concentration,
In the case of house cultivation in step 4, the nutrient source is treated and the morel mushroom fruiting body is grown by adjusting the temperature to 5 to 20 ° C and relative humidity to 80 to 90%,
The nutritional source of step 4 is characterized in that it includes 1.5 parts by weight of quicklime, 1.5-2.5 parts by weight of gypsum, 0.005-0.015 parts by weight of KH 2 PO 4 , and 2.5-7.5 parts by weight of white sugar.
Cultivation method of morel mushroom fruiting body.
상기 단계 1의 배양된 곰보버섯균을 곰보버섯 자실체 인공재배용 배지 조성물에 접종하는 단계(단계 2);
상기 접종된 곰보버섯균을 20 내지 25℃의 배양온도, 60 내지 70%의 상대습도 조건에서 25 내지 30일간 배양하여 곰보버섯균으로부터 균핵을 형성하는 단계(단계 3); 및
상기 형성된 균핵을 상자 재배 및 하우스 재배 중 어느 하나의 버섯 재배 환경 조건의 토양에 접종한 후, 토양 표면에 균사체 생육이 만연하게 되면 영양원을 처리하고, 곰보버섯 자실체를 생육하는 단계(단계 4); 를 포함하고,
상기 단계 2의 곰보버섯 자실체 인공재배용 배지 조성물은 참나무 발효톱밥 100 중량부 기준, 부엽토 13.56-14.99 중량부, 밀 27.14-29.99 중량부, 생석회 2.03-2.24 중량부, 석고 2.70-2.99 중량부, KH2PO4 0.013-0.015 중량부 및 백설탕 6.78-7.49 중량부를 포함하는 것을 특징으로 하고,
상기 단계 4의 상자 재배의 경우 영양원을 처리하고 10 내지 20℃의 온도, 60 내지 95% 상대습도 및 800 내지 1000ppm 이산화탄소 농도로 조절하여 곰보버섯 자실체를 생육하는 것을 특징으로 하고,
상기 단계 4의 하우스 재배의 경우 영양원을 처리하고 5 내지 20℃의 온도 및 80 내지 90% 상대습도로 조절하여 곰보버섯 자실체를 생육하는 것을 특징으로 하며,
상기 단계 4의 영양원은 생석회 1.5 중량부 기준, 석고 1.5-2.5 중량부, KH2PO4 0.005-0.015 중량부 및 백설탕 2.5-7.5 중량부를 포함하는 것을 특징으로 하는,
곰보버섯 자실체의 재배방법.Inoculating and culturing morel mushroom bacteria in a medium mixed with sawdust and rice bran (step 1);
Inoculating the cultured morel mushroom bacteria of step 1 into a medium composition for artificial cultivation of morel mushroom fruiting bodies (step 2);
Culturing the inoculated morel mushroom bacteria for 25 to 30 days at a culture temperature of 20 to 25°C and relative humidity of 60 to 70% to form sclerotia from the morel mushroom bacteria (step 3); and
Inoculating the formed sclerotia into soil under either mushroom cultivation environmental conditions of box cultivation or house cultivation, then processing the nutrient source when mycelium growth becomes rampant on the soil surface, and growing morel mushroom fruiting bodies (step 4); Including,
The medium composition for artificial cultivation of morel mushroom fruiting bodies in step 2 includes 100 parts by weight of fermented oak sawdust, 13.56-14.99 parts by weight of humus, 27.14-29.99 parts by weight of wheat, 2.03-2.24 parts by weight of quicklime, 2.70-2.99 parts by weight of gypsum, and KH 2. Characterized in that it contains 0.013-0.015 parts by weight of PO 4 and 6.78-7.49 parts by weight of white sugar,
In the case of box cultivation in step 4, the nutrient source is treated and the morel mushroom fruiting body is grown by adjusting the temperature to 10 to 20°C, 60 to 95% relative humidity, and 800 to 1000 ppm carbon dioxide concentration,
In the case of house cultivation in step 4, the nutrient source is treated and the morel mushroom fruiting body is grown by adjusting the temperature to 5 to 20 ° C and relative humidity to 80 to 90%,
The nutrient source of step 4 is characterized in that it includes 1.5 parts by weight of quicklime, 1.5-2.5 parts by weight of gypsum, 0.005-0.015 parts by weight of KH 2 PO 4 , and 2.5-7.5 parts by weight of white sugar.
Cultivation method of morel mushroom fruiting body.
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| WO2017056059A1 (en) | 2015-10-01 | 2017-04-06 | Bagnato Maurizio | Method of producing mushrooms |
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| WO2017056059A1 (en) | 2015-10-01 | 2017-04-06 | Bagnato Maurizio | Method of producing mushrooms |
| US20180000013A1 (en) | 2016-02-26 | 2018-01-04 | Functional Fungi, Llc | Enhanced Mycelium Growth Medium and Method |
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