KR101602718B1 - Culture medium for cultivating cauliflower mushroom and cultivating mehtod for cauliflower mushroom using the same - Google Patents

Abstract

The present invention relates to a culture medium for cultivating Sparassis crispa, which includes 100 parts by weight of needleleaf tree sawdust, 5 to 20 parts by weight of corn powder, 5 to 20 parts by weight of flour, 5 to 25 parts by weight of ground pozzolan and 160 to 300 parts by weight of water, wherein the water is present in an amount of 55 to 65 wt% with respect to the total weight of the culture medium for cultivating Sparassis crispa.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a medium for cultivating a mushroom, and a method for cultivating the mushroom using the same. BACKGROUND OF THE INVENTION 1. Field of the Invention [0002]

The present invention relates to a medium for cultivating a mushroom and a method of cultivating mushroom using the same. More specifically, it is possible to apply a medium containing pozzolan to a culture medium containing a mushroom mushroom which has higher mushroom occurrence rate, faster growth rate, lower cultivation cost, superior taste, and reduced contamination rate, Cultivation medium and a method for cultivating the mushroom of the present invention.

Sparassis crispa is a mushroom of mushroom, mushroom, mushroom and mushroom. The fruit body is a flower-shaped white flower and has a shape of 10 to 25 cm in diameter. It is also called edible mushroom because it has a chewy taste and is like a pine mushroom that has a flower-shaped smell like a pine mushroom. These mushrooms are found on the ground near the roots of coniferous trees such as pine, pine, larch, beech and buckwheat pine trees, or on brown stems, which are found on the stump, in July and September in the wild. do. The above mushroom contains more than 40g of β-glucan per 100g of dried mushroom, so it has been recently introduced as a mushroom which is very good for improving immunity.

Unlike ordinary cultivated mushrooms, Zanthoxylum mushroom is grown using coniferous trees such as larch, pine and pine. In this regard, Japanese Patent Laid-Open Publication No. 11-56098 discloses a method for cultivating a Japanese mushroom by using a medium supplemented with a nutrient source after hot water extraction of the larchus to remove water components, and Korean Patent Publication No. 2002 -48337 discloses a method in which activated carbon and active calcium are added to a medium in which a mixture of lanthanum and light leaves is mixed and sterilized at a high temperature and a high pressure in a medium supplemented with a commonly used nitrogen source, A method of artificial cultivation of Japanese mushroom is disclosed.

However, the growth conditions of the mushroom are difficult and the growth period of the mushroom is slow, so mass production is not easy.

On the other hand, pozzolan minerals are a kind of volcanic ash, which is known as a material produced in the Cretaceous period. It is a mineral originating from Pozori village near the island of Bari, Italy. Other distribution areas are produced only in extremely limited regions such as the United States of America, Malaysia, and India. In Asia, there is no equal vein in the vast plains of China.

It is an object of the present invention to provide a culture medium for growing mushroom which can increase mushroom occurrence rate and mushroom production.

Another object of the present invention is to provide a culture medium for cultivation of mushroom which is low in cultivation cost and can be shortened in cultivation period.

Another object of the present invention is to provide a culture medium for growth of mushroom which is excellent in taste and can reduce the contamination rate.

It is another object of the present invention to provide a method for cultivating a mushroom of the mushroom by using the medium for cultivation of the mushroom.

It is another object of the present invention to provide a mushroom grown using the medium for cultivating the mushroom.

One aspect of the present invention relates to a medium for cultivating a mushroom. The medium for growing the mushroom of the present invention comprises 100 parts by weight of softwood sawdust, 5 to 20 parts by weight of corn flour, 5 to 20 parts by weight of wheat flour, 5 to 25 parts by weight of pozzolana crumbs and 160 to 300 parts by weight of water, And 55 to 65% by weight based on the total weight of the mushroom culture medium.

In one embodiment, the coniferous sawdust may be one obtained from one or more of the following: Larch, Pine, Pine, Pine, Rigida pine, Rigida pine, Pine and Cedar.

In one embodiment, the average size of the softwood sawdust and the pozzolan mill may be 1.5 mm to 5 mm.

In one embodiment, the density of the medium for cultivating the mushroom is 0.6 g / cm3 to 1.5 g / cm3.

Another aspect of the present invention relates to a method for cultivating a mushroom of Brassica juncea using the medium for cultivating the mushroom. In a specific example, the cultivation method comprises: sterilizing a container filled with the culture medium for cultivating mushroom cultivation; A step of inoculating a mushroom strain to a culture medium for sterilization of the mushroom cultivar; And culturing the inoculated strains of P. aeruginosa.

Another aspect of the present invention relates to a mushroom cultivated using the medium for cultivating the mushroom.

The mushroom cultivated by applying the culture medium for the growth of the mushroom according to the present invention can increase the incidence and production rate of the mushroom of the mushroom and the growth rate of the mushroom is low and the cultivation period Can be shortened, the texture and taste at the time of chewing are excellent, and the contamination rate can be reduced.

Fig. 1 is a test report measuring the content of germanium (Ge) contained in the pozzolan pulverized product.
Fig. 2 is a test report measuring the far-infrared emissivity and radiant energy of the pozzolan pulverized product.
Fig. 3 is a test report showing the results of antibacterial tests on E. coli and P. aeruginosa of pozzolan pulverized material.
Fig. 4 is a photograph showing comparison of the results before and after the antibacterial test with Pseudomonas aeruginosa in the pozzolana crushed product.
Fig. 5 shows the results of antibacterial tests on E. coli and P. aeruginosa of the pozzolan powder.
6 is a test report showing the result of the antifungal test of the pozzolan pulverized product.
Fig. 7 is a photograph showing the cultivation process of the mushroom of the present invention using the culture medium for the growth of the mushroom of the present invention.
FIG. 8 is a photograph showing the foliage mushroom fruit body grown using the culture medium for the growth of the mushroom of the present invention.

In the following description of the present invention, a detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are intended to be exemplary, self-explanatory, allowing for equivalent explanations of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the accompanying drawings.

One aspect of the present invention relates to a medium for cultivating a mushroom. In one embodiment, the medium for cultivating the mushroom is softwood sawdust, corn meal, wheat flour, pozzolana milled water and water. In an embodiment, the medium for cultivating the mushroom of the present invention comprises 100 parts by weight of softwood sawdust, 5 to 20 parts by weight of corn flour, 5 to 20 parts by weight of wheat flour, 5 to 25 parts by weight of pozzolan powder and 160 to 300 parts by weight of water.

 The water is contained in an amount of 55 to 65% by weight based on the total weight of the medium for cultivating the mushroom.

The above-mentioned softwood sawdust can be used in the fields of Larix kaemferi C, Pinus densiflora , Pinus thunbergii Parl., Pinus rigida Mill., Pinus rigida P. taeda hybrid, Pinus pinus coraiensis ) and cedar ( Cryptomeria japonica ).

In the concrete example, the softwood sawdust may be used in which the larch and the pine forest are contained in a weight ratio of 1: 0.2 to 0.5. When the sawdust is used in the weight ratio, the growth rate of the mycelial growth of mycelia and the productivity of the fruiting body of mushroom can be improved.

In an embodiment, the average size of the softwood sawdust may be between 1.5 mm and 5 mm. Here, the " size " of the softwood sawdust means the maximum length. When the average size of softwood sawdust is applied, the growth rate of hypha and the productivity of the mushroom can be improved.

The corn flour is included as a nutrient source for mycelial growth of the mushroom. The corn flour is included in an amount of 5 to 20 parts by weight based on 100 parts by weight of the softwood sawdust. And 10 to 20 parts by weight in the specific examples. When the corn flour is contained in an amount of less than 5 parts by weight, the mycelial growth rate of the mushroom is lowered. When the corn flour is contained in an amount exceeding 20 parts by weight, the synergistic effect of nutrient supply is insufficient compared to the growth rate of mycelial growth and the amount of corn flour, .

The wheat flour is included as a nutrient source for mycelial growth of the mushroom. Conventional flour may be used. For example, one or more of high strength, gravity strength and impact strength can be used. The flour is contained in an amount of 5 to 20 parts by weight based on 100 parts by weight of the softwood sawdust. And 10 to 20 parts by weight in the specific examples.

When the wheat flour is contained in an amount of less than 5 parts by weight, the mycelial growth rate of the mushroom is lowered. When the wheat flour is contained in an amount exceeding 20 parts by weight, the synergistic effect of nutrient supply is insufficient.

Fig. 1 is a test report measuring the content of germanium (Ge) contained in the pozzolana pulverized product, Fig. 2 is a test report measuring far-infrared emissivity and radiant energy of the pozzolana pulverized product, This is the test report showing the results of the antibacterial test. 1 to 3, the pozzolana crushed material includes components such as germanium (Ge), promotes mycelial growth of the mushroom, has excellent far-infrared emission effect, increases mushroom production rate, And is included for the purpose of reducing the contamination rate because of excellent sterilizing effect and excellent antibacterial property against Pseudomonas aeruginosa, E. coli and fungi.

The pozzolan crushed material of the present invention can be used by pulverizing natural pozzolan minerals. In an embodiment, the pozzolana crushed material is contained in an amount of 5 to 25 parts by weight based on 100 parts by weight of the softwood sawdust. In the specific examples, 10 to 25 parts by weight are included.

When the amount of the pozzolan powder is less than 10 parts by weight, the effect of shortening the growth time of the mushroom and reducing the contamination rate of the mushroom is lowered. When the amount of the pozzolan powder is more than 25 parts by weight, I do not.

The average size of the pozzolanized pulverized product may be 1.5 mm to 5 mm. Here, the " size " of the pozzolan milled product means the maximum length.

The water is included for mycelial growth and mixing of the components. The water is contained in an amount of 160 to 300 parts by weight based on 100 parts by weight of the softwood sawdust. When the water is contained in an amount of less than 160 parts by weight, the mixing property is deteriorated. When the water is contained in an amount exceeding 300 parts by weight, mycelial growth may not be properly performed. For example, 200 parts by weight to 285 parts by weight.

The water is included in an amount of 55 to 65% by weight based on the total weight of the medium for cultivating the mushroom. When the water (moisture) is less than 55% by weight based on the total weight of the culture medium for the mushroom cultivation, mycelial growth is not properly performed. When the water content exceeds 65% by weight, mycelial growth may not be properly performed.

In an embodiment, the density of the culture medium for cultivating the mushroom may be 0.6 g / cm3 to 1.5 g / cm3. For example, 0.7 g / cm 3 to 1.2 g / cm 3. It is excellent in air permeability in the above density range, and the growth rate of the mycelium of mycelium of mushroom and the yield of the mushroom of the mushroom can be excellent.

The culture medium for cultivating the mushroom of the present invention can be produced by a conventional method. In a specific example, the medium for cultivation of the mushroom is 160-300 parts by weight of water in 100 parts by weight of softwood sawdust, 5-20 parts by weight of corn powder, 5-20 parts by weight of wheat flour and 5-25 parts by weight of pozzolana powder, And the water is prepared so as to be contained in an amount of 55 to 65% by weight based on the total weight of the medium for cultivating the mushroom cultivar.

Another aspect of the present invention relates to a method for cultivating a mushroom of Rosemary mushroom using a medium for cultivating a mushroom. In one embodiment, the method of cultivating the mushroom is (a) a sterilization step; (b) a step of infecting the mushroom strain; And (c) a culturing step. More specifically, the step of sterilizing the container filled with the culture medium for growth of the mushroom cultivation described above; A step of inoculating a mushroom strain to a culture medium for sterilization of the mushroom cultivar; And culturing the inoculated strains of P. aeruginosa.

Hereinafter, the method of cultivating the mushroom of the present invention will be described step by step.

(a) Sterilization step

The above step is a step of sterilizing the container filled with the culture medium for cultivating the mushroom cultivar. In a specific example, the medium for cultivating the mushroom is 1 to 160 parts by weight of water, 100 parts by weight of the above-mentioned softwood sawdust, 5 to 20 parts by weight of corn flour, 5 to 20 parts by weight of wheat flour and 5 to 25 parts by weight of pozzolana ground powder. By weight and the water is contained in an amount of 55 to 65% by weight based on the total weight of the medium for cultivating the mushroom.

In a specific example, the culture medium for cultivating the mushroom of the present invention can be filled in the container and sterilized. In the specific example, the culture medium for cultivating mushroom of the present invention can be packed at a uniform density of 45 to 70% by volume in a bottle-shaped container. As the container, a bottle made of heat-resistant polypropylene can be used.

Sterilization of the culture medium for the growth of the mushroom cultivar is carried out by removing all the germs in the medium for cultivation of the mushroom culture and changing the composition of the culture medium for the mushroom culture to a form easy to use and promoting the growth of the hyphae by softening the physical properties .

The sterilization can be performed by closing the lid of the container filled with the culture medium for the mushroom cultivation and sterilization at a temperature of 100 ° C to 130 ° C and a pressure of 1.0 to 1.5 atm for 90 minutes to 150 minutes. Under the above conditions, all the germs in the mixed medium can be removed, and the components of the medium can be changed into a form in which hyphae can be easily used, thereby promoting mycelial growth.

In addition, after the sterilization, the container filled with the medium for cultivating the mushroom may be cooled to 15 to 25 ° C.

(b) Stage of Inoculation with Mushroom Strain

The above step is a step of inoculating a bryophyta mushroom strain to a sterilized growth medium for growth of mushroom. The above-mentioned Rosemary mushroom strain may be any conventional one. For example, KFRI 644, KFRI 645, KFRI 691, KFRI 700, KFRI 720, KFRI 722, KFRI 723, KFRI 724, KFRI 746, KFRI 747, KFRI 748 or KFRI 749 can be used.

In a specific example, liquid culture can be used. For example, strains isolated from wild-type mushroom fruiting bodies are cultured in a PDB (potato dextrose broth) medium for 20 to 35 days at 20 to 25 ° C., then cultured in PDB medium for 20 to 35 days The mushroom strain cultivated under the condition of 20 ~ 25 ℃ can be inoculated into the growth medium for the mushroom cultivation.

The mushroom strain may be inoculated in an amount of 1 to 20 parts by weight based on 100 parts by weight of the sterilized mushroom cultivation medium. When inoculated in the above range, the productivity of P. japonicus can be excellent.

(c) Culture step

In this step, the inoculated mushroom strain is cultured to induce a fruiting body, followed by cultivation thereof to obtain a fruit body. In an embodiment, the culturing is carried out by first cultivating the inoculated infantile mushroom strain at a temperature of 15 ° C to 23 ° C and a humidity of 75% to 90% for 40 to 70 days; And cultivating the primary cultivated P. japonicus strain at a temperature of 15 ° C to 23 ° C and a humidity of 75% to 90% for 25 to 45 days; Step < / RTI >

As described above, the mycelium is spread over the entire culture medium through the first cultivation, and the first cultivated myrtle mushroom strain is transferred to the cultivation house, and then the lid of the container is removed and the second culture is performed to obtain a fruit body. The productivity of the foliage mushroom fruiting body can be excellent while preventing the occurrence of fungi upon culturing under the above conditions. For example, primary cultivation for 40 to 60 days at a temperature of 18 to 20 DEG C and a humidity of 85% to 90%; And culturing the primary cultivated P. japonicus strain at a temperature of 18 to 20 ° C and a humidity of 85% to 90% for 30 to 40 days.

Specifically, the time of harvesting can be determined by checking the color of the mushroom or the elasticity of the flower-shaped fruiting body. Generally, the young mushroom has a wrinkle-like shape, and the cultivated mushroom has a color close to white with a light yellowish color, It can be determined not only that the corolla becomes deeply cauliflower like a wave, but also because the elasticity decreases as the harvesting time approaches.

Another aspect of the present invention relates to a mushroom cultivated by the method of cultivating the mushroom and cultivated using the medium for cultivating the mushroom. The mushroom cultivated by applying the culture medium for the growth of the mushroom according to the present invention can increase the incidence and production amount of the mushroom of the mushroom, and the cultivation cost is low, and the cultivation period Can be shortened, the texture and taste at the time of chewing are excellent, and the contamination rate can be reduced.

Hereinafter, the configuration and operation of the present invention will be described in more detail with reference to preferred embodiments of the present invention. However, the following examples are provided to aid understanding of the present invention, and the scope of the present invention is not limited to the following examples. The contents not described here are sufficiently technically inferior to those skilled in the art, and a description thereof will be omitted.

Example

Fruit mushroom fruiting body, which grows naturally in the vicinity of the base of 40- to 50 - year - old old tree of Pinus koraiensis in Gangwon - do area, was harvested and grown on PDA (Potato Dextrose Agar) using tissue isolation method. Then, the strain was cultured in a PDB (potato dextrose broth) medium at 20 to 25 ° C for 20 to 35 days, and then cultured in PDB medium for 20 to 35 days at 20 to 25 ° C The mushroom strain was prepared.

100 parts by weight of the above-mentioned softwood sawdust, 15 parts by weight of corn flour, 15 parts by weight of wheat flour (gravity fraction) and 15 parts by weight of pozzolan powder having an average size of 1.5 mm And 20 parts by weight of water were mixed to prepare a first mixture, and 280 parts by weight of water was mixed with the first mixture to prepare a culture medium for growth of Mushroom mushroom containing 65% by weight of water. At this time, the density of the culture medium for the growth of the mushroom was 0.9 g / cm 3.

20 pieces of heat-resistant polypropylene 1000ml-size bottles were prepared, and each bottle was filled with 650g of the medium for cultivating the mushroom. The bottle was then closed, and the bottle was closed with a vacuum sterilization apparatus (autoclave) The container filled with the culture medium for growth of the mushroom cultivation for 90 minutes at a temperature of 120 DEG C and a pressure of 1.2 atm was sterilized.

20 ml of the prepared mushroom strain was inoculated in the sterilized mushroom cultivation medium and the inoculated mushroom strain was first cultivated at a temperature of 18 to 23 캜 and a humidity of 85 to 90% The first cultivated P. japonicus mushroom strain was transferred to a grower, and then the bottle cap was removed from the bottle filled with the medium for cultivating mushroom cultivation and then the temperature was raised to 18 to 22 ° C and the humidity of 75% to 90% And the fruit body was collected when the end of the fruiting body was formed into a flower cabbage shape.

Comparative Example 1

The fruiting body of the mushroom was obtained by the same method as in Example except that a medium containing no pozzolana was used.

Comparative Example 2

The fungus mushroom fruit bodies were harvested by the same method as in Example except for using a medium containing 2 parts by weight of pozzolana pulverized to 100 parts by weight of the softwood sawdust.

Comparative Example 3

The fungus mushroom fruit bodies were collected by culturing in the same manner as in Example 1 except that the medium containing 30 parts by weight of the pozzolana pulverized product was applied to 100 parts by weight of the softwood sawdust.

The average period of cultivation (primary cultivation + secondary cultivation) required for cultivation of the mushroom fruiting bodies of the above Examples and Comparative Examples 1 to 3 and harvesting thereof, the yield of fruiting body mushroom fruiting body and the fruiting body mushroom occurrence rate Are shown in Table 1 below.

division The amount of fruiting body mushroom fruiting body per bottle (g) Average cultivation period (days) Fruit body formation rate (%) Example 205 98 85 Comparative Example 1 176 122 60 Comparative Example 2 188 114 65 Comparative Example 3 191 113 65

Fig. 1 is a test report measuring the content of germanium (Ge) contained in the pozzolan pulverized product, Fig. 2 is a test report measuring far-infrared emissivity and radiant energy of the pozzolana pulverized product, FIG. 4 is a photograph showing the result of comparing the results of the antimicrobial test with the pseudomonas aeruginosa of the pozzolana powder before and after the antimicrobial test, and FIG. 5 is a photograph showing the results of the antimicrobial test of E. coli and P. aeruginosa And FIG. 6 is a test report showing the result of the antifungal test of the pozzolana crushed product. FIG. 7 is a photograph showing the process of cultivating the mushroom of the present invention using the culture medium for the growth of the mushroom of the present invention, and FIG. 8 is a photograph showing the mushroom of the mushroom cultivated using the culture medium for the growth of the mushroom of the present invention.

Referring to Table 1 and FIGS. 1 to 8, it was found that the pozzolan powder of the present invention contains germanium which is useful for mushroom cultivation, has excellent far-infrared radiation characteristics, and contains antibacterial effect. The mushroom cultivated by the medium of the Example was prepared in the same manner as in Comparative Example 1, which was cultivated using the medium containing the pozzolana milled product, and Comparative Example 1, which was cultivated using the medium containing the pozzolana milled product The growth rate of mushroom fruiting bodies was high and the productivity of fruiting mushroom fruiting bodies was excellent.

Claims (6)

100 parts by weight of softwood sawdust, 15-20 parts by weight of corn powder, 15-20 parts by weight of wheat flour, 20-25 parts by weight of pozzolan crushed powder and 160-300 parts by weight of water,
Wherein the water is contained in the softwood sawdust, the corn meal, the wheat flour, the pozzolan mill and the water in an amount of 55 to 65 wt%
Wherein the coniferous sawdust comprises larch and Liga pine at a weight ratio of 1: 0.2-0.5.
The medium for cultivating mushroom cultivation according to claim 1, which comprises 100 parts by weight of the softwood sawdust, 15 parts by weight of corn flour, 15 parts by weight of wheat flour, 20 parts by weight of pozzolana millet, and 280 parts by weight of water.
[3] The medium for cultivating mushroom cultivation of claim 1, wherein the average size of the softwood sawdust and the pozzolana crushed material is 1.5 mm to 5 mm.
[3] The culture medium for cultivating mushroom according to claim 1, wherein the density of the medium for cultivating the mushroom is 0.6 g / cm3 to 1.5 g / cm3.
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