JP2008193969A - Culture medium for artificial cultivation of lyophyllum - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an artificial cultivation method cultivating Lyophyllum at a high yield in a short period without causing various germs and contamination, unlike a conventional method, and enabling commercial production. <P>SOLUTION: A culture medium for artificial cultivation of Lyophyllum contains grains subjected to blasting treatment. It is preferable that the blasting treatment is operation of emission in low pressure after pressurizing and heating in the presence of saturated steam with pressure of 0.2-2.0 MPa and a temperature of 121-160°C, and the grains are barley or rice plant grains. The artificial cultivation method of Lyophyllum comprising using the culture medium for artificial cultivation is also provided. <P>COPYRIGHT: (C)2008,JPO&INPIT

Description

  The present invention relates to a culture medium for artificial cultivation of shimeji and a method for artificial cultivation of shimeji.

  Mushrooms, including basidiomycetes and some ascomycetes, are not only used for foods, but are also attracting attention for their physiological activities and are recognized as industrially useful materials in a wide range of fields including pharmaceuticals. (For example, refer nonpatent literatures 1 and 2.).

  The fungus bed cultivation technology currently used for the cultivation of edible mushrooms is that the inoculum of the mushrooms to be cultivated is extremely clean in an artificial cultivation environment where the temperature, humidity, illuminance, oxygen concentration, etc. are carefully controlled.・ Pure cultivation under clean conditions. In addition, as the base material of the culture medium used, broad-leaved trees such as Kunugi and Quercus, conifers such as cedar, corn cob, etc. are used, and various materials such as bran, rice bran, soybean meal, and bean husk are added as nutrients to be added. The mushroom cultivation method using food residue etc. is illustrated also as test research.

  It is also known that the water content of the culture medium, the water retention capacity of the culture medium, so-called water potential, affects the formation of fruit bodies, the yield, etc. (see, for example, Non-Patent Documents 3 to 8).

  When these conventional artificial cultivation techniques are carried out in industrial scale mushroom cultivation using a large amount of fungus beds, the water content and water potential may be low depending on the culture medium used. Water has accumulated in the area, causing contamination with bacteria, generation of mites, and the like, resulting in a reduction in the quality of the mushrooms. In addition, there were some differences in cultivation results depending on the fungus bed, and the time of formation of mushroom fruit body primordial varieties differed by the fungus bed unit, resulting in a long harvest period.

  In the artificial cultivation of wood-rotting fungi such as shiitake, oyster mushrooms, sea cucumbers, enokitake and maitake, which are generally considered edible, industrial cultivation methods have been carried out by devising various cultivation methods.

  Moreover, about the shimeji represented by "aroma matsutake taste shimeji", it has been reported about various artificial cultivation methods until now (for example, refer patent documents 1-4, nonpatent literature 9-12).

  However, in the technical field, the water content accompanying the water potential has been stabilized, the labor required for stabilizing the water content of the culture medium used for mushroom cultivation and the improvement of the cultivation efficiency, in other words, industrial The uniformization of the fungus bed has always been an issue, and the essential solution has not yet been found.

On the other hand, regarding the conventional cultivation of mushrooms by processing existing materials and changing the culture medium without changing the water potential, the artificial cultivation method in which the substrate itself used for the culture medium is processed and processed has been reported. However, for example (see Patent Documents 5 and 6), no method has been known for processing and processing a nutrient material used for a culture medium.
1171415591812_0, "Using mushrooms-from disease treatment and prevention to environmental improvement", Jinshokan, April 2006 1171415591812_1, “Scientific Mushrooms: From Shiitake to Agaricus Blazei,” Jinjinshokan, May 2001 Hu Changqing, Satoshi Meguro, Susumu Kawachi, “Effect of water potential of wood medium on growth of edible fungi”, Abstracts of Annual Meeting of the Japan Wood Research Society, March 2002, Vol. 52, p. 427 Hu Changqing, Meguro Sadatoshi and Kawauchi Susumu, “Adjustment of Edible Bacterial Cultivation Conditions by Balance of Water Activity and Water Potential”, Abstracts of Annual Meeting of the Wood Society of Japan, March 2003, Vol. 53, p. 698 Culture maturity of Lentinula edodes on sawdust-based substrate in relation to fruiting potential. (Ohga, S., Min, D.-S, Koo, C.-D., Choi, TH., Leonowicz, A., Cho, N .-S.,), Mokchae Konghak, 28: 55-64 (2000) Effect of water potential on fruit body formation of Lentinula edodes in sawdust-based substrate. (Ohga, S.), J. Wood Sci., 45: 337-342 (1999) Water potential in relation to culture maturity in sawdust-based substrate of Lentinula edodes. (Ohga, S., Katoh, Y., Nakaya, M.), Mushroom Sci. Biotechnol., 6: 65-69 (1998) Togashi, Minamio Serizawa, "Cultivation test of genus Narakes using water-absorbing material", Forestry Experiment Station Report, Hokkaido Forestry Experiment Station, March 1993, Vol. 7, No. 2, p. 7-9 Japanese Patent Publication No. 08-004427 Japanese Patent Laid-Open No. 2001-120059 JP 2000-106752 A Japanese Patent Application Laid-Open No. 2002-247917 “Rinkeikyo Monthly Report”, No. 422, July 1998, p. 29-34 “The Mycological Society of Japan”, Vol. 39, No. 1, 1998, p. 13-20 "Shiga Prefectural Forest Center Business Report", Volume 34, 2001, p. 1-3 “Mushroom Yearbook 2004”, 2004, Plants World, p. 202-203 Japanese Patent Publication No.55-22078 Japanese Patent Publication No.59-55122

  The present invention provides a culture medium suitable for artificial cultivation of shimeji mushrooms, which can stably maintain the moisture content and has high air permeability, and by using the culture medium, mushrooms have a good fruiting body forming ability. The purpose of the present invention is to provide a commercial artificial cultivation method that can reduce the number of days of cultivation without causing contamination with bacteria.

  As a result of intensive investigations to solve such problems, the present inventors have included in the culture medium what is obtained by subjecting cereals to blasting treatment, and by artificially cultivating shimeji using this culture medium, The present inventors have found the fact that they can be cultivated in a short period of time and that the average yield is increased.

  That is, the first of the present invention is based on a culture medium for artificial cultivation of shimeji mushroom characterized by containing cereals subjected to blasting treatment. Preferably, the blasting treatment is performed at a pressure of 0.2 MPa to 2.MPa. In the presence of saturated water vapor at 0 MPa and a temperature of 121 to 160 ° C., pressurizing and heating and then releasing under low pressure, and preferably the cereal is a grain such as barley or rice It is a culture medium for artificial cultivation of the shimeji.

  The second aspect of the present invention is a method for artificially cultivating shimeji mushroom characterized by inoculating the above-mentioned culture medium for artificial cultivating shimeji with inoculum of shimeji, performing artificial cultivation, and harvesting the fruiting body generated. It is.

  According to the present invention, the blasted cereals are solidified with a sufficient gap so that air permeability is high and water does not accumulate in the lower part of the fungus bed, so that contamination of bacteria and other bacteria is not seen, and shimeji Good fruit bodies can be harvested in large quantities in shorter cultivation days. In addition, since the crushed cereals are also used as nutrients, it is not necessary to add additives such as horticultural soil such as red crust and kanuma soil, which are not necessary for the growth of mushrooms. In the above possibilities, the added value is high.

  Hereinafter, the present invention will be specifically described.

  Cereals used in the present invention include barley (Hordeum vulgare L. var. Hexastlchon Aschers.) Rice (Oryza sativa L.), wheat (Triticum aestivum L.), rye (Secale cereale L.), beer wheat (Hordeum distichum L .), Pearl barley (Coix lacryma-jobi L. Var. Frumentacea Makino), etc., and is not limited to the country of origin, place of origin, or food, processing, feed, etc. Commercially available germinated rice, germinated wheat, and the like can also be suitably used. Of these, barley, which is commercially available as edible food, can be suitably used. For example, Hakubaku or Nippon Seiwa Co., Ltd. can be purchased and used. Can do.

  In the present invention, it is necessary to perform the blasting treatment on the cereals as described above, but the part to be subjected to the blasting treatment is preferably a part of the grain produced in the ear of the cereal plant. Moreover, as a magnitude | size of a grain, it is a granular body which has a major axis of 3-15 mm, Preferably it is 3.5-12 mm, More preferably, it is 5.0-10 mm.

  The blasting process in the present invention refers to a process of pulverizing the cereal by placing the cereal under pressure for a certain period of time and then instantaneously returning it to normal pressure. Specifically, it refers to an operation in which cereals are placed in a heat-resistant container, held at a predetermined pressure for a predetermined time in the presence of water vapor, and then rapidly released to atmospheric pressure. The pressurization includes a method of pressurizing the inside of the container with a compressor, a method of pressurizing with high-temperature and high-pressure steam, and a method of pressurizing by pressurizing the sealed container, and a method of pressurizing the sealed container is preferable.

  The pressure is usually 0.1 to 2.0 MPa, more preferably 0.2 to 1.8 MPa, and most preferably 0.5 to 1.5 MPa in terms of gauge pressure.

  The time for pressurization and heating under the above conditions is 3 minutes to 15 minutes, preferably 4 minutes to 12 minutes, and more preferably 5 minutes to 10 minutes.

  The apparatus used for the blasting treatment is not particularly limited as long as the treatment can be performed under the above conditions. For example, the product name “Electric Pon Confectionery Machine No. 11” manufactured by Tachibana Kiko Co., Ltd. is preferable. Available.

  Grains are pulverized by the blasting treatment as described above, and the shape is a granular material having a porous structure having a major axis of 3.5 to 20 mm, preferably 3.8 to 18 mm, more preferably 4.0 to 16 mm. A blasted cereal with a porous structure can be obtained.

  The moisture content of the cereal subjected to the explosion treatment is 4.5 to 30% by mass, preferably 4.8 to 25% by mass, and more preferably 5 to 22% by mass. If the water content is out of the above range, the water content may be adjusted by a treatment such as hot air drying, vacuum drying, freeze vacuum drying or the like.

  A specific example of the explosion treatment in the present invention will be described in the case of barley. Barley (Hakubaku Co., Ltd.) 1500-1600g (moisture content is 17% by mass) is put into the product name “Electric Pon Confectionery Machine No.11” manufactured by Tachibana Kiko Co., Ltd., and the gauge pressure is 1.2-1.3 MPa. The blasting barley having a porous structure can be obtained by heating the cereals for 15 to 20 minutes until the doors of the high-pressure sealed kettle are rapidly opened and exposing the crushed cereals to the air. Barley is marketed under the names of barley, wheat cracking, compacted pressed wheat, ground cracking, etc. for food and feed, but they can also be suitably used as long as they can be crushed by the above conditions.

  The present invention is characterized in that the above-mentioned cereals subjected to a blasting treatment are included in the culture medium for artificial cultivation. Only a part of the cereal after the blasting treatment may be included, or the rice husks and germs may be attached or removed. The amount of cereals that have been subjected to blasting treatment to be included in the culture medium is affected by the base material and other nutrients that are included in other cultures, but roughly the volume ratio of the entire culture medium is included in the blasted cereals: others. The base material and nutrients are preferably 1: 0.1 to 0.8, more preferably 1: 0.2 to 0.6, and most preferably 1: 0.3 to 0.5.

  In this invention, it is preferable to add what further functions as a base material to the culture medium for artificial cultivation. As such, the base material conventionally used in the fungus bed cultivation of a general mushroom is used suitably, specifically, sawdust, corn cob, sugarcane squeezed bagasse, and a fiber mass of coconut. More preferred is sawdust. The sawdust may be coniferous or broadleaf derived, but preferably, it is a coniferous pine family (Pinaceae), cedar family (Taxodiaceae), and broadleaf trees are bayberry family (MYRICACEAE), walnut family (JUGLANDACEAE). , Mulaceae, TROCHODENDRACEAE, Fusazakura (EUPTELEACEAE), Katsura (CERCIDIPHYLLACEAE) Akebidae (LARDIZABALACEAE), Barberry (BERBERIDACEAE), Mokuren (Mokuren), ACE Family (SAXIFRAGACEAE), Toberidae (PITTOSPORACEAE), Witch Hazel (HAMAMELIDACEAE), Sphagnum (PLATANACEAE), Rose (ROSACEAE), Legume (FABACEAE), Citrus (RUTACEAE), Sendai (MELIACEAE), Euphorbiaceae EUPHORBIACEAE), Boxwood (BUXACEAE), Aquinophylaceae (AQUIFOLIACEAE), Asteraceae (CELASTRACEAE), Adenaceae (HIPPOCASTANACEAE), SABIACEAE, Aceraceae (RHAMNACEAE), Grapeaceae (VITACEAE), Aoiaceae (MALVACEAE), Matatabidae (ACTINIDIACEAE), Camelliaaceae (THEACEAE), Irigiriaceae (FLACOUTIACEAE), Kibushiidae (STACHYURACEAE) (ELAEGNACEAE), Misohidae (LYTHRACEAE), Pomegranate (PUNICACEAE), Arukiaceae (ARALIACEAE), Mizuki (CORNACEAE), Ryeaceae (CLETHRACEAE), Azalea (ERICACEAE), Yabukouji (MYRSINACEAE), ENACEAE (EB) ), Hygiaceae (SYMPLOCACEAE), Egonaceae (STYRACACEAE), Oxaceae (OLEACEAE), Oleander (APOCYNACEAE), Anemoneaceae (VERBENACEAE), Nozenkazura (BIGNONIACEAE), Rubiaceae (RUBIACEAE), CAP More preferably, willow family (SALICACEAE), beech family (Fragaceae), linden family (TILIACEAE), maple family (ACERACEA) E) Sawdust derived from plants classified into birch family (BETULACEAE) and elmaceae (ULMACEAE) can be preferably used. The particle size of the sawdust is 0.2 to 7 mm, preferably 0.25 to 5 mm, and more preferably 0.3 to 3 mm.

  The content of these base materials is preferably crushed cereals: base material = 1: 0.1 to 1.0 by volume ratio, more preferably 1: 0.2 to 0.8, and most preferably 1: 0. .4 to 0.6. In addition, the volume ratio by the difference in the particle size of a base material is not specifically limited in this invention. Moreover, the moisture content of what is used for the base material is 5 to 40% by mass, preferably 5 to 25% by mass, and more preferably 5 to 20% by mass.

  In the present invention, cereals subjected to blasting treatment contain a large amount of starch when grains are used, and thus function as nutrients necessary for the growth of shimeji mushrooms. Therefore, the culture medium for artificial cultivation of the present invention may not contain nutrients in addition to the cereals that have been subjected to the explosion treatment, but preferably, nutrients used for fungus bed cultivation of general edible mushrooms are added. Is good. Examples of such nutrients include bran, okara, soybean meal, beer lees, seaweeds, etc., and these shapes preferably have a granular shape with a major axis of 1.0 mm or more, and more preferably Has a diameter of 1.0 to 60 mm, more preferably 1.5 to 5.9 mm, and most preferably 1.6 to 5.8 mm. As an added amount of these nutrients, it is preferable that, by volume ratio, crushed cereals: other nutrient sources = 1: 0.1 to 0.5, more preferably 1: 0.1 to 0.4, and most preferably. Is preferably 1: 0.1 to 0.2. In addition, the volume ratio by the difference in the particle size of the raw material used as a nutrient is not particularly limited in the present invention. Moreover, the moisture content of what is used for the raw material of a nutrient is 5-40 mass%, More preferably, it is 5-25 mass%, Most preferably, the dry state of 5-20 mass% is desirable.

  An example of the production method of the culture medium for artificial cultivation of the present invention is given below.

  Grains subjected to the blasting treatment to be included in the culture medium for artificial cultivation of the present invention, the base material of the culture medium, and, if necessary, a predetermined amount of nutrients are weighed, stirred, and added to adjust moisture.

  Next, the second method for artificially cultivating shimeji of the present invention will be described. The method for artificially cultivating shimeji of the present invention can employ the conventional method for artificially cultivating shimeji, except that the above-described culture medium for artificial cultivation of the first shimeji of the present invention is used. As the type of cultivation method, fungus bed cultivation such as bottle cultivation, bag cultivation, toro box cultivation and the like can be applied. As an example, the procedure for bottle cultivation is described as follows: production of culture medium, bottle filling of culture medium, sterilization, inoculation with shimeji mushroom, culture, sprouting operation, growth, and harvesting. Next, although these are demonstrated concretely, this invention is not limited to these.

  First, the production of the culture medium is as described in the first method for producing the culture medium for artificial cultivation of the present invention.

  The culture medium bottle filling is a process of filling the prepared culture medium into a bottle. Usually, the culture medium is packed in a heat-resistant wide-mouth culture bottle having a capacity of 400 to 2300 ml, and the culture medium is 500 to 800 g, preferably 600 to 750 g in the case of an 850 ml bottle. And a process of making a hole of about 1 to 3 cm in the center and plugging it. In the present invention, one hole may be formed in the culture medium, or two or more holes may be formed in the center. More preferably, it is three or more.

  The sterilization may be a step of killing all microorganisms in the culture medium with steam, and is usually 98-100 ° C. for 4-12 hours for normal pressure sterilization, 101-125 ° C. for high pressure sterilization, preferably 121 ° C., 30 ~ 90 minutes.

  Inoculation with shimeji inoculum is a process of inoculating inoculum in a culture medium that has been allowed to cool after sterilization. As a typical inoculum, shimeji mycelium is used in an agar medium such as PDA (potato dextrose) (Wako Pure Chemical Industries, Ltd.) agar medium. Cultivated at 30 ° C for 30-60 days, mixed with beech sawdust at a volume ratio of 3 and barley at a volume ratio of 2, sterilized by the above method, aseptically planted, and cultured at 25 ° C for 60-150 days After that, use the bacteria. This is aseptically planted about 20 g per bottle.

  Culture is a process of growing and maturing mycelia, in which the inoculated culture medium (mycelium bed) is spread at a temperature of 20 to 25 ° C. and a humidity of 40 to 70%, and further matured. Aging can be omitted. In the case of an 850 ml bottle, the culture step is usually performed for 60 to 150 days, preferably about 80 days.

  The sprouting operation is a step of removing the stopper after completion of the culturing step to form a fruiting body primordium, usually 10 to 20 ° C., preferably around 15 ° C., humidity 80% or more, and an illuminance of 500 to 1000 lux. Perform for 20 days. At this time, the fungus floor may be covered with a suitable material. Examples of the soil covering material include horticultural soil such as red jade soil, peat moss, and Kanuma soil, soil for inorganic minerals such as natural soil, perlite, and vermiculite, and plant-derived materials such as rice husk and sphagnum.

  These soil covering materials may be used alone or in combination. Further, the soil covering material may be used after absorbing water in advance so as to have an appropriate moisture content. In addition, after removing the stopper, an operation such as scratching / bacterial pushing or scraping of the bun type fungus may be performed to damage the inoculum part and the surface of the fungus bed. In addition, during the sprouting step, dew condensation is likely to occur due to humidification, so that the fungus floor may be covered with newspaper, nonwoven fabric or the like for the purpose of preventing wetting. In addition, as a sprouting step, an operation for urging the bottle to turn upside down may be performed.

  Growth is a process of forming a mature fruit body from the fruit body primordium, and is carried out for 10 to 15 days under substantially the same conditions as the normal sprouting process. A mature fruit body can be obtained by the above process, harvesting is performed, and all the processes of cultivation are complete | finished.

  As mentioned above, although this invention was demonstrated by the bottle cultivation method, it is possible to implement by said method also in bag cultivation and toro box cultivation.

  Next, a shimeji strain suitable for the artificial cultivation method of the present invention will be described. Examples of the strain of shimeji include the genus Lyophyllum, Pleurotus, genus Lepista, and Tricholoma, and are recognized by the Japanese standard name in Japan. shimeji), O. shimeji (L. connatum), L. sykosporum, L. semitale, L. fumosum, L. anthracophilum, L. tylicolor, L. tylicolor decastes), oyster mushrooms (P. ostreatus), Himalayan oyster mushrooms (P. sajor-caju), eringi (P. eryngii), tomato oyster mushrooms (P. djamor), P. cornucopiae, L. nuda In addition, all the mushrooms of the genus Shimeji, Oyster mushrooms, Murasaki medusa and Kishimeji are included in addition to L. sordida and T. giganteum. These strains include isolates from wild fruiting bodies, commercially available strains by inoculum manufacturers, preserved strains of public institutions, etc., and mutants, mating strains, cell fusion strains, etc. of these strains. All the strains having

  Specifically, as hon-shimeji, NBRC100035 preserved as a microorganism specimen strain by the National Institute of Technology and Evaluation, NBRC30662 as shakashimeji, NBRC30484 as purple shimeji, ATCC-90762 as shimeji shimeji As oyster mushrooms, Kitaken H7 sold by Kitaken Co., Ltd., and eringi include KE-106 strains sold by Katsuragi Sangyo Co., Ltd. For example, it may be a stock strain of a public institution or an isolated strain from the wild.

  Hereinafter, the present invention will be specifically described by way of examples. The blasting process is performed based on the method of 0017 described above, and will be described in the case of barley. The blasting treatment was performed using Tachibana Kiko Co., Ltd. (trade name “Electric Pon Confectionery Machine No. 11”). As a condition of the blasting treatment, 1550 g of barley (Hakubaku Co., Ltd.) is placed in a container (water content is 17% by mass) and heated for 17 minutes until the gauge pressure becomes 1.2 MPa. Explosion-treated barley having a porous structure was obtained by a process of rapidly opening and exposing the barley to the air.

Example 1
PDA (potato dextrose) medium (Wako Pure Chemical Industries, Ltd.) was inoculated with mycelium of hon-shimeji (NBRC100035), pre-cultured at 25 ° C. for 30 days, and the agar medium was used as an inoculum. Boiled barley (purchased from Kyoto Yamashiro Agricultural Cooperative) and beech sawdust (Arai Shoten Co., Ltd.) are mixed at a dry matter volume ratio of 3: 2, so that the water content of the culture medium is finally 60%. The culture medium of the present invention was prepared by adding water to the mixture and thoroughly stirring and mixing.

  The obtained culture medium was packed into 850 ml of polypropylene oyster mushroom culture bottles (Chikuma Kasei Co., Ltd., 850 cc—diameter 58 mm), clogged, and a hole having a diameter of about 3 cm was made in the center, and then plugged and sealed at 120 ° C. for 60 minutes High-pressure steam sterilization was performed and the mixture was allowed to cool.

  The 32 agar culture seeds were inoculated with the above-mentioned agar medium inoculum, and the mycelium was cultured in the dark at a temperature of 23 ° C. and a humidity of 60 to 70% for 30 days to spread the hyphae throughout the entire mycelium bed. Next, after removing the stopper and covering the bottle mouth with soil sterilized by autoclaving, the bottle is moved to a generation room where the temperature is 15 ° C. and the humidification is controlled to 85 to 100%, and continues to grow under illumination of 500 to 1500 lux. Got a mature fruiting body. The average yield per bottle of the obtained mature fruiting body was 99.5 g. The average number of days required for harvest after inoculation was 79 days.

Comparative Example 1
Culturing was carried out until mature fruit bodies were obtained in the same manner as in Example 1, except that the barley was used without being subjected to the explosion treatment. The average yield obtained per bottle was 81 g. The average number of days required for harvest after inoculation was 95 days.

Example 2
Cultivation was carried out until mature fruit bodies were obtained in exactly the same manner except that Shakashimeji (NBRC30662) strain was used in Example 1. The average yield per bottle of the fruiting body obtained was 80.5 g. The average number of days required for harvest after inoculation was 88 days.

Comparative Example 2
Culturing was carried out until mature fruit bodies were obtained in the same manner as in Example 2, except that the barley was used without being subjected to the explosion treatment. The average yield per bottle was 30.7 g. The average number of days required for harvest after inoculation was 102 days.

Example 3
Cultivation was carried out until mature fruit bodies were obtained in the same manner as in Example 1, except that the Murasakiximeji (NBRC30484) strain was used. The average yield of mature fruit bodies per bottle of the fruit bodies thus obtained was 95 g. The average number of days required for harvest after inoculation was 125 days.

Comparative Example 3
Cultivation was carried out until mature fruit bodies were obtained in exactly the same manner as in Example 3, except that the barley was used without being subjected to the blasting treatment. The average yield of mature fruit bodies per bottle of the fruit bodies thus obtained was 50.3 g. The average number of days required for harvest after inoculation was 162 days.

Example 4
Cultivation was carried out until mature fruit bodies were obtained in the same manner as in Example 1, except that the strain S. cerevisiae (ATCC-90762) was used. The average yield per bottle of mature fruit bodies per bottle of the fruit bodies obtained was 40.5 g. The average number of days required for harvest after inoculation was 110 days.

Comparative Example 4
Culturing was carried out until mature fruit bodies were obtained in the same manner as in Example 4 except that the barley was used without being subjected to the explosion treatment. The average yield per bottle of the obtained fruiting bodies was 25.4 g per bottle of the obtained mature fruiting body. The average number of days required for harvest after inoculation was 121 days.

Example 5
Cultivation was carried out until mature fruit bodies were obtained in exactly the same manner except that the oyster mushroom (Hokuken H7) strain was used in Example 1. The average yield per bottle of the fruiting body obtained was 90.5 g. The average number of days required for harvest after inoculation was 45 days.

Comparative Example 5
Culturing was carried out until mature fruit bodies were obtained in the same manner as in Example 5, except that the barley was used as it was without being subjected to the explosion treatment. The average yield per bottle of the fruiting body obtained was 80.2 g. The average number of days required for harvest after inoculation was 50 days.

Example 6
Cultivation was carried out until mature fruit bodies were obtained in the same manner as in Example 1 except that Eringhi KE-106 strain was used. The average yield per bottle of the fruiting body obtained was 84.3 g. The average number of days required for harvest after inoculation was 63 days.

Comparative Example 6
Culturing was carried out until mature fruit bodies were obtained in the same manner as in Example 6, except that the barley was used as it was without being subjected to the explosion treatment. The average yield per bottle of the fruiting body thus obtained was 79.8 g. The average number of days required for harvest after inoculation was 77 days.

Example 7
In Example 1, rice (purchased from Kyoto Yamashiro Agricultural Cooperative) was used instead of barley, and the blasting treatment was performed in the same manner as barley. Explosive-treated rice and beech sawdust were mixed at a dry matter volume ratio of 3: 2, and cultivated until a mature fruit body was obtained in the same manner as in Example 1. The average yield per bottle of the fruiting body obtained was 84.6 g. The number of days required for harvest after inoculation was 85 days.

Comparative Example 7
Cultivation was carried out until mature fruit bodies were obtained in the same manner as in Example 7, except that rice was used as it was without being subjected to blasting treatment. The average yield per bottle of the fruiting body obtained was 80.4 g. The number of days required until harvest after inoculation was 80 days.

Example 8
In Example 7, cultivation was carried out until mature fruit bodies were obtained in exactly the same manner except that rice and beech sawdust (Arai Shoten Co., Ltd.) were mixed at a dry matter volume ratio of 1: 1. The average yield per bottle of the fruiting body thus obtained was 76.2 g. The number of days required for harvest after inoculation was 96 days.

Comparative Example 8
Cultivation was carried out until mature fruit bodies were obtained in the same manner as in Example 8, except that rice was used without being subjected to blasting treatment. The average yield per 1 bottle of the fruit body obtained was 60.0 g. The number of days required for harvest after inoculation was 102 days.

Example 9
Cultivation was carried out until mature fruit bodies were obtained in exactly the same manner except that the oyster mushroom (Hoken No. H7) strain was used in Example 8. The average yield of the fruiting body per bottle was 115.6 g. The number of days required until harvest after inoculation was 54 days.

Comparative Example 9
Culturing was carried out until mature fruit bodies were obtained in the same manner as in Example 9, except that rice was used as it was without being subjected to blasting treatment. The average yield per bottle of the fruiting body obtained was 98.4 g. The number of days required for harvest after inoculation was 61 days.

Example 10
Cultivation was carried out until mature fruit bodies were obtained in exactly the same manner except that Elinghi (KE-106) strain was used in Example 8. The average yield per 1 bottle of the fruiting body obtained was 142.0 g. The number of days required until harvest after inoculation was 67 days.

Comparative Example 10
Cultivation was carried out until mature fruit bodies were obtained in the same manner as in Example 10 except that rice was used without being subjected to blasting treatment. The average yield of the fruiting body per bottle was 112.4 g. The number of days required for harvest after inoculation was 88 days.
The results obtained above are shown in Table 1.

Claims (5)

A culture medium for artificial cultivation of shimeji mushroom, characterized by containing cereals subjected to a blasting treatment. The artificial cultivation of shimeji mushroom according to claim 1, wherein the blasting treatment is an operation of pressurizing and heating in the presence of saturated steam at a pressure of 0.2 MPa to 2.0 MPa and a temperature of 121 to 160 ° C, and then releasing it under a low pressure. For culture medium. The culture medium for artificial cultivation of shimeji mushroom according to claim 1 or 2, wherein the cereal is barley or rice. The culture medium for artificial cultivation of shimeji mushroom according to any one of claims 1 to 3, wherein the cereal is a cereal grain. A method for artificially cultivating shimeji mushroom, comprising inoculating the culture medium for artificial cultivating shimejime according to any one of claims 1 to 4 with an inoculum of shimeji, performing artificial cultivation, and harvesting the fruiting body.