JPH0478276B2 - - Google Patents

Info

Publication number
JPH0478276B2
JPH0478276B2 JP56164064A JP16406481A JPH0478276B2 JP H0478276 B2 JPH0478276 B2 JP H0478276B2 JP 56164064 A JP56164064 A JP 56164064A JP 16406481 A JP16406481 A JP 16406481A JP H0478276 B2 JPH0478276 B2 JP H0478276B2
Authority
JP
Japan
Prior art keywords
dna
coli
sak
phage
add
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP56164064A
Other languages
English (en)
Japanese (ja)
Other versions
JPS5867181A (ja
Inventor
Tomoyuki Sako
Saeko Sawaki
Toshizo Sakurai
Masahiko Mutai
Isamu Kondo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yakult Honsha Co Ltd
Original Assignee
Yakult Honsha Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yakult Honsha Co Ltd filed Critical Yakult Honsha Co Ltd
Priority to JP56164064A priority Critical patent/JPS5867181A/ja
Priority to KR8204582A priority patent/KR910001807B1/ko
Priority to CA000413486A priority patent/CA1206109A/en
Priority to DK460182A priority patent/DK164001C/da
Priority to EP82305508A priority patent/EP0077664B1/en
Priority to US06/434,503 priority patent/US4532211A/en
Priority to DE8282305508T priority patent/DE3280175D1/de
Publication of JPS5867181A publication Critical patent/JPS5867181A/ja
Priority to JP2295382A priority patent/JPH03198775A/ja
Publication of JPH0478276B2 publication Critical patent/JPH0478276B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP56164064A 1981-10-16 1981-10-16 スタフイロキナーゼ産生遺伝情報を担うｄｎａ断片組み換え体ｄｎａおよびそれを導入した大腸菌 Granted JPS5867181A (ja)

Priority Applications (8)

Application Number Priority Date Filing Date Title
JP56164064A JPS5867181A (ja) 1981-10-16 1981-10-16 スタフイロキナーゼ産生遺伝情報を担うｄｎａ断片組み換え体ｄｎａおよびそれを導入した大腸菌
KR8204582A KR910001807B1 (ko) 1981-10-16 1982-10-12 스태필로키나아제를 생산하는 유전자를 함유하는 새로운 대장균 및 스태필로키나아제의 제조법
CA000413486A CA1206109A (en) 1981-10-16 1982-10-15 E. coli having gene for production of staphylokinase and process for production of staphylokinase therewith
DK460182A DK164001C (da) 1981-10-16 1982-10-15 Rekombinant-dna, der kan udtrykke staphylokinase og er afledt fra en temperat phag af staphylococcus aureus, fremgangsmaade til fremstilling af rekominant-dna samt fremgangsmaade til fremstilling af staphylokinase
EP82305508A EP0077664B1 (en) 1981-10-16 1982-10-15 Recombinant dna and coliform bacillus having genetic material for the production of staphylokinase, and the production of staphylokinase
US06/434,503 US4532211A (en) 1981-10-16 1982-10-15 Coliform bacillus having a gene for the production of staphylokinase and a process for production of staphylokinase therewith
DE8282305508T DE3280175D1 (de) 1981-10-16 1982-10-15 Rekombinante dns und coliformer bazillus genetisches material enthaltend zur herstellung von staphylokinase und die herstellung von staphylokinase.
JP2295382A JPH03198775A (ja) 1981-10-16 1990-11-02 スタフイロキナーゼの製造法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP56164064A JPS5867181A (ja) 1981-10-16 1981-10-16 スタフイロキナーゼ産生遺伝情報を担うｄｎａ断片組み換え体ｄｎａおよびそれを導入した大腸菌

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2295382A Division JPH03198775A (ja) 1981-10-16 1990-11-02 スタフイロキナーゼの製造法

Publications (2)

Publication Number Publication Date
JPS5867181A JPS5867181A (ja) 1983-04-21
JPH0478276B2 true JPH0478276B2 (enExample) 1992-12-10

Family

ID=15786090

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56164064A Granted JPS5867181A (ja) 1981-10-16 1981-10-16 スタフイロキナーゼ産生遺伝情報を担うｄｎａ断片組み換え体ｄｎａおよびそれを導入した大腸菌

Country Status (7)

Country Link
US (1) US4532211A (enExample)
EP (1) EP0077664B1 (enExample)
JP (1) JPS5867181A (enExample)
KR (1) KR910001807B1 (enExample)
CA (1) CA1206109A (enExample)
DE (1) DE3280175D1 (enExample)
DK (1) DK164001C (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4764469A (en) * 1984-03-02 1988-08-16 Board Of Regents For The University Of Oklahoma Streptokinase-coding recombinant vectors
AU561372B2 (en) * 1983-10-10 1987-05-07 Board Of Regents Of The University Of Oklahoma, The Streptokinase-coding recombinant vectors
US5683909A (en) * 1984-03-02 1997-11-04 Van Den Bergh Foods Company, Division Of Conopco, Inc. Plasmids replicatable in Bacillus subtilis, E. coli and lactic acid streptococcus bacteria
US5066589A (en) * 1984-03-02 1991-11-19 Board Of Regents Of The University Of Okla. Streptokinase-coding recombinant vectors
IL76422A0 (en) * 1984-09-26 1986-01-31 Lilly Co Eli Method for expression and secretion in bacillus
IT1176997B (it) * 1984-10-17 1987-08-26 Anic Spa Procedimento per la produzione di proteasi neutra
ES2061982T5 (es) * 1988-04-14 1999-03-16 Yakult Honsha Kk Activador de plasminogeno y agente trombolitico.
EP0621899B1 (de) * 1991-12-30 1996-06-19 Medac Gesellschaft für klinische Spezialpräparate mbH Expression signalpeptid-freier staphylokinasen
CN1035192C (zh) * 1994-04-04 1997-06-18 上海医科大学 一种重组葡激酶的制备方法
US5951980A (en) * 1995-01-06 1999-09-14 Leuven Research & Development Vzw Identification, production and use of new staphylokinase derivatives with reduced immunogenicity
US6383483B1 (en) 1995-01-06 2002-05-07 Désiré José Collen Staphylokinase derivatives with cysteine substitutions
RU2156299C1 (ru) * 1999-02-03 2000-09-20 Закрытое акционерное общество "Фосфосорб" Рекомбинантная плазмидная днк, кодирующая полную последовательность стафилокиназы, штамм escherichia coli sa 9325 - продуцент рекомбинантного белка

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4366246A (en) * 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
JPS54107589A (en) * 1978-02-09 1979-08-23 Ajinomoto Co Inc Separation and purification of staphylokinase

Also Published As

Publication number Publication date
CA1206109A (en) 1986-06-17
EP0077664A2 (en) 1983-04-27
DK164001C (da) 1992-09-21
KR910001807B1 (ko) 1991-03-26
EP0077664B1 (en) 1990-05-16
DK460182A (da) 1983-04-17
DE3280175D1 (de) 1990-06-21
KR840002029A (ko) 1984-06-11
DK164001B (da) 1992-04-27
JPS5867181A (ja) 1983-04-21
EP0077664A3 (en) 1984-05-02
US4532211A (en) 1985-07-30

Similar Documents

Publication Publication Date Title
US5004692A (en) Cloning and expression of phosopholipase C genes
Clark-Curtiss et al. Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae
US4359535A (en) Autonomously replicating DNA containing inserted DNA sequences
JPH0478276B2 (enExample)
US20090087421A1 (en) Novel Recombinant Staphylokinase Derivatives and the Preparations and Applications thereof
US4348478A (en) Method for preparation of a recombinant DNA phage
Rogers et al. Identification and analysis of a gene (abpA) encoding a major amylase-binding protein in Streptococcus gordonii
EP0567503B1 (en) Pasteurella haemolytica glycoprotease gene
JP2000074922A (ja) シュードモナス・アエルギノザの外部膜タンパク質ｆ
Rosenow et al. Characterization and localization of the KpsE protein of Escherichia coli K5, which is involved in polysaccharide export
Mallavia et al. The genetics of Coxiella burnetii: etiologic agent of Q fever and chronic endocarditis
US6676943B1 (en) Human complement C3-degrading protein from Streptococcus pneumoniae
JPH0479640B2 (enExample)
JPH0387188A (ja) フィブリン結合ドメインを有するハイブリッドストレプトキナーゼおよびその製造方法
Reddy et al. Isolation, cloning and expression of recombinant staphylokinase gene against thrombosis
Han et al. The Porphyromonas gingivalis prtP/kgp homologue exists as two open reading frames in strain 381
EP0420894A1 (en) METHOD FOR PURIFYING LARGE PURE HEPARINASE ON A LARGE SCALE.
RU2127758C1 (ru) Способ получения рекомбинантной стрептокиназы
JPH0520067B2 (enExample)
KR100544594B1 (ko) 스트렙토콕커스 뉴모니애로부터의 사람 보체 ｃ3-분해 프로테이나아제
RU2156299C1 (ru) Рекомбинантная плазмидная днк, кодирующая полную последовательность стафилокиназы, штамм escherichia coli sa 9325 - продуцент рекомбинантного белка
KR100384467B1 (ko) 한국산 지렁이에서 분리한 룸부로키나제 유전자 및 효모에서 룸부로키나제 단백질 대량 발현 방법
Chandrappa et al. Production, characterization and evaluation of thrombolytic activity of staphylokinase of Staphylococcus hominis
Kuykindoll et al. Characterization of a P1-deficient strain of Streptococcus mutans that expresses the SpaA protein of Streptococcus sobrinus
RU2373281C2 (ru) Способ продукции рекомбинантной стафилокиназы при регулировании уровня кислорода