JPH0430279B2 - - Google Patents

Info

Publication number
JPH0430279B2
JPH0430279B2 JP60502509A JP50250985A JPH0430279B2 JP H0430279 B2 JPH0430279 B2 JP H0430279B2 JP 60502509 A JP60502509 A JP 60502509A JP 50250985 A JP50250985 A JP 50250985A JP H0430279 B2 JPH0430279 B2 JP H0430279B2
Authority
JP
Japan
Prior art keywords
dna
rna
pathogen
support
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP60502509A
Other languages
English (en)
Japanese (ja)
Other versions
JPS61502376A (ja
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of JPS61502376A publication Critical patent/JPS61502376A/ja
Publication of JPH0430279B2 publication Critical patent/JPH0430279B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Pathology (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP60502509A 1984-06-11 1985-06-06 未確認の病原体或いは遺伝子実体を生体外にて検出し同定する方法 Granted JPS61502376A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US619286 1984-06-11
US06/619,286 US4652517A (en) 1984-06-11 1984-06-11 Methods for the in vitro detection and identification of unknown pathogens or genetic entities

Publications (2)

Publication Number Publication Date
JPS61502376A JPS61502376A (ja) 1986-10-23
JPH0430279B2 true JPH0430279B2 (https=) 1992-05-21

Family

ID=24481252

Family Applications (1)

Application Number Title Priority Date Filing Date
JP60502509A Granted JPS61502376A (ja) 1984-06-11 1985-06-06 未確認の病原体或いは遺伝子実体を生体外にて検出し同定する方法

Country Status (6)

Country Link
US (1) US4652517A (https=)
EP (1) EP0188450B1 (https=)
JP (1) JPS61502376A (https=)
AT (1) ATE79474T1 (https=)
AU (1) AU594925B2 (https=)
WO (1) WO1986000139A1 (https=)

Families Citing this family (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5288611A (en) * 1983-01-10 1994-02-22 Gen-Probe Incorporated Method for detecting, identifying, and quantitating organisms and viruses
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
US4806631A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on solvolyzed nylon supports
US5985549A (en) * 1985-10-22 1999-11-16 University Of Massachusetts Non-isotopic in-situ hybridization method for detection of nucleic acids
US4888278A (en) * 1985-10-22 1989-12-19 University Of Massachusetts Medical Center In-situ hybridization to detect nucleic acid sequences in morphologically intact cells
EP0261955A3 (en) * 1986-09-26 1989-06-07 E.I. Du Pont De Nemours And Company Process for immobilization of dna
US7087742B1 (en) 1986-11-24 2006-08-08 Gen-Probe Incorporated Oligonucleotide probes for the detection and/or quantitation of non-viral organisms
US7090972B1 (en) 1986-11-24 2006-08-15 Gen-Probe Incorporated Methods for determining the presence of non-viral organisms in a sample
US4977251A (en) * 1987-01-30 1990-12-11 University Of Illinois Board Of Trustees DNA probes for identification of bacteroides species
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
DE3850273T2 (de) * 1987-03-02 1994-09-29 Gen Probe Inc Polykationische Träger zur Reinigung, Trennung und Hybridisierung von Nukleinsäure.
IL85948A0 (en) * 1987-04-08 1988-09-30 Cambridge Bioscience Corp Method and kit for detecting bacteria or fungi
AU1701488A (en) * 1987-04-08 1988-11-04 Invitron Corporation Detection and identification of dna and/or rna in protein-containing samples
EP0311388B1 (en) * 1987-10-06 1995-02-22 Ortho Diagnostic Systems Inc. A rapid method for identifying a specific nucleic acid sequence
US5294533A (en) * 1988-07-05 1994-03-15 Baylor College Of Medicine Antisense oligonucleotide antibiotics complementary to the macromolecular synthesis operon, methods of treating bacterial infections and methods for identification of bacteria
GR1002090B (en) * 1988-09-30 1995-12-28 Ortho Diagnostic Systems Inc A rapid method for identifying a specific nucleic acid sequence
US6242184B1 (en) 1988-10-18 2001-06-05 University Of Massachusetts In-situ hybridization of single-copy and multiple-copy nucleic acid sequences
US7172863B1 (en) 1988-12-09 2007-02-06 Gen-Probe Incorporated Nucleic acid probes and methods for detecting Neisseria gonorrhoeae
ATE157404T1 (de) * 1989-03-17 1997-09-15 Abbott Lab Verfahren und vorrichtung zur hybridisierung von nukleinsäure mit verbesserter reaktionskinetik
DE3919393A1 (de) * 1989-06-14 1990-12-20 Hoechst Ag Verfahren zur stabilisierung von auf festphasen immobilisierten biologisch aktiven substanzen
US5106730A (en) * 1989-07-24 1992-04-21 Microprobe Corporation Lactam-containing compositions and methods useful for the hybridization of nucleic acids
US5700636A (en) * 1990-10-19 1997-12-23 Becton Dickinson And Company Methods for selectively detecting microorganisms associated with vaginal infections in complex biological samples
DK0554355T3 (da) * 1990-10-19 1998-05-11 Microprobe Corp Fremgangsmåder og farmaceutiske kits til anvendelse ved detektering af mikroorganismer forbundet med vaginalinfektioner
IL102486A (en) * 1991-10-04 1997-11-20 Orgenics Ltd Method and apparatus for detection of nucleic acid sequences with a nucleic acid probe
US5284771A (en) * 1991-12-05 1994-02-08 Miles Inc. Reagent compositions and their use in sphering cells
US5350695A (en) * 1991-12-05 1994-09-27 Miles Inc. Methods for the identification and characterization of reticulocytes in whole blood
DE4212555A1 (de) * 1992-04-15 1993-10-21 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Nukleinsäuren
CA2139847C (en) * 1992-07-07 2002-05-21 Akio Matsuhisa Probe for diagnosing infectious disease
US6045996A (en) * 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US5658749A (en) * 1994-04-05 1997-08-19 Corning Clinical Laboratories, Inc. Method for processing mycobacteria
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
ES2110916B1 (es) * 1996-05-24 1998-10-01 Ivia Procedimiento de preparacion de dianas para la reaccion en cadena de la polimerasa (pcr).
US5985593A (en) * 1996-10-11 1999-11-16 Integrated Research Technology, L.L.C. Compositions and methods for enzymatic decontamination
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
GB2338236B (en) * 1998-06-13 2003-04-09 Aea Technology Plc Microbiological cell processing
WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
DE19956820A1 (de) * 1999-11-25 2001-05-31 Roche Diagnostics Gmbh Spezies-spezifischer Nachweis von Nukleinsäuren mittels eines Analyseelementes
DE10137342A1 (de) * 2001-07-31 2003-03-06 Infineon Technologies Ag Biosensor und Verfahren zum Erfassen von makromolekularen Biopolymeren mittels mindestens einer Einheit zum Immobilisieren von makromolekularen Biopolymeren
US20040180445A1 (en) * 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
US7150995B2 (en) * 2004-01-16 2006-12-19 Metrika, Inc. Methods and systems for point of care bodily fluid analysis
US7972872B2 (en) * 2004-06-07 2011-07-05 Denka Seiken Co., Ltd. Chromatography detection apparatus, detection method, and kit utilizing the same
US7883855B2 (en) * 2006-07-21 2011-02-08 Abbott Laboratories Immunosuppressant drug extraction reagent for immunoassays
CA2673314C (en) * 2006-12-29 2014-03-11 Abbott Laboratories Non-denaturing lysis reagent for use with capture-in-solution immunoassay
CA2673296C (en) * 2006-12-29 2012-10-16 Abbott Laboratories Improved assay for immunosuppressant drugs
WO2008082979A2 (en) * 2006-12-29 2008-07-10 Abbott Laboratories Diagnostic test for the detection of a molecule or drug in whole blood
US7914999B2 (en) * 2006-12-29 2011-03-29 Abbott Laboratories Non-denaturing lysis reagent
EP2636756B2 (en) 2008-05-27 2019-11-20 Dako Denmark A/S Compositions and methods for detection of chromosomal aberrations with novel hybridization buffers
EP2401396B1 (en) 2009-02-26 2016-11-23 Dako Denmark A/S Methods for performing a stringent wash step in hybridization applications
EP2333105A1 (en) 2009-12-08 2011-06-15 Koninklijke Philips Electronics N.V. Selective lysis of cells
WO2013046033A1 (en) * 2011-09-30 2013-04-04 Dako Denmark A/S Hybridization compositions and methods using formamide
EP2768974B1 (en) 2011-10-21 2017-07-19 Dako Denmark A/S Hybridization compositions and methods
US10072284B2 (en) * 2012-06-21 2018-09-11 Monsanto Technology Llc Lysis buffer and methods for extraction of DNA from plant material
KR102535489B1 (ko) 2014-06-13 2023-05-22 큐-리네아 에이비 미생물 검출 방법 및 특성 규명 방법
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
US10428370B2 (en) * 2016-09-15 2019-10-01 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample
US11959125B2 (en) 2016-09-15 2024-04-16 Sun Genomics, Inc. Universal method for extracting nucleic acid molecules from a diverse population of one or more types of microbes in a sample

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
US4407942A (en) * 1981-01-29 1983-10-04 Atomic Energy Of Canada Limited Fluorescent detection of DNA damage
US4689295A (en) * 1983-01-20 1987-08-25 Integrated Genetics, Inc. Test for Salmonella
GB8332525D0 (en) * 1983-12-06 1984-01-11 Secr Defence Conformable fascine

Also Published As

Publication number Publication date
WO1986000139A1 (en) 1986-01-03
EP0188450A4 (en) 1989-02-02
US4652517A (en) 1987-03-24
ATE79474T1 (de) 1992-08-15
EP0188450B1 (en) 1992-08-12
AU594925B2 (en) 1990-03-22
AU4431385A (en) 1986-01-10
JPS61502376A (ja) 1986-10-23
EP0188450A1 (en) 1986-07-30

Similar Documents

Publication Publication Date Title
JPH0430279B2 (https=)
CN110541022B (zh) 基于CRISPR-Cas12a系统的结核分枝杆菌复合群检测试剂盒
JP3866762B2 (ja) 広範な生物からの核酸抽出法
US5348855A (en) Assay for nucleic acid sequences in an unpurified sample
EP2347011B1 (en) Fast results hybrid capture assay and system
JPS60100056A (ja) 核酸ハイブリダイゼ−シヨン法による細菌の検出方法
US4358535A (en) Specific DNA probes in diagnostic microbiology
DE3586660T2 (de) Hybridisierungstest fuer nukleinsaeure.
JP2012506705A5 (https=)
JPS60262055A (ja) 核酸のハイブリツド形成分析法およびそれに用いる試薬系
JPS60110299A (ja) ｔｕｆ及びｆｕｓ遺伝子プロ−ブを用いる核酸ハイブリダイゼ−シヨンによる細菌の検出方法
US5747256A (en) Homogeneous DNA probe titration assay
Yehle et al. A solution hybridization assay for ribosomal RNA from bacteria using biotinylated DNA probes and enzyme-labeled antibody to DNA: RNA
JPH0743376B2 (ja) 核酸配列の検定方法及び分子遺伝子プローブ
AU2016231944B2 (en) Isolation of nucleic acids
JP6108661B2 (ja) 配列特異的な大量試料調製方法およびアッセイ法
CN119604626A (zh) 使用CRISPR RNA组合和Cas13a酶进行病毒诊断
CN119372340B (zh) 引物对和探针在制备检测犬虫媒性病原微生物的试剂盒中的应用
WO2013117968A1 (en) Methods for lysing bacteria from sample and isolating cellular components therefrom, and kits therefor
DE3586490T2 (de) Verfahren zum in vitro-nachweis und bestimmung unbekannter pathogene oder genetischer einheiten.
CA1314794C (en) Assay for nucleic acid sequence in a sample
Harding Microbiology: Microbial Antigen Detection
CN117144026A (zh) 一种检测解脲脲原体的居家自检试剂盒
JPH037582A (ja) Ｄｎａ回収方法