JPH04152825A - Method for proliferating clone seedling and clone bulb (rhizome) of begonia tuberhybrida and device therefor - Google Patents

Abstract

PURPOSE:To proliferate the title plant in enhanced proliferation ratio without applying high load to a mother free by using petiole of Begonia Tuberhybrida provided with leaf and free from bud, main stem thereof provided with leaf and left lamina of nerve part as a scion and germinating and growing the scion using a plant hormone to afford a seedling. CONSTITUTION:Petiole of Begonia Tuberhybrida provided with leaf and free from bud, main stem thereof provided with leaf or left lamina of nerve part of Begonia Tuberhybrida are used as a scion and a mixture of auxin and cytokinin which is a sort of plant hormone is attached to the scion and the scion is put on an electric carriage 7 in a heat insulating house 5 and a door 8 of a culture device is opened and the electric carriage 7 is run on a rail 6 and the carriage is put on a lift 3 in a housing chamber surrounded with heat insulating wall 1 and raised and fixed with a movable hook 4 provided in a lateral posts and then the door 8 is closed and scion is cultured while keeping the inside in proper temperature by a cooling and heating device 2 and control device 9 to produce germination point and the scion is germinated and rooted to provide the seedling. Thereby clone seedling and clone bulb of Begonia Tuberhybrida are proliferated.

Description

DETAILED DESCRIPTION OF THE INVENTION [Field of Industrial Application] The present invention relates to a technique for propagating genetically uniform bulbous begonia seedlings and bulbs (tubers), and an apparatus therefor.

[Conventional technology] Conventional methods for propagating bulbous begonias include side bud cuttings, apical bud cuttings,
There are tissue culture methods and bulb division methods. bulb (tuber)
In order to enter dormancy for generation, the natural daylight hours are 10
They took advantage of the autumn climate, where the temperature drops to below 20 degrees Celsius at night.

To force sleep, use blackout curtains to reduce daylight hours by 8-
I was doing short-day operations to 10 hours.

[Problems to be Solved by the Invention] Conventional propagation of bulbous begonia clones, such as tomata, is generally done by propagating leaf cuttings, such as those of -parts begonias, because even if they take root, no germination points are formed. The stems and leaves of a certain part were used as cuttings, and the cuttings were propagated. Therefore, the locations from which cuttings can be harvested are limited, making it difficult to propagate in large quantities.

In the conventional cutting method, the germination point of the cutting is not a rooting force, so during dormancy after a short period of growth, the tissue part of the stem and the starchy bulb (tuber) part get mixed up and the seedling grows. The long-term preservation of the bulb (tuber) itself was poor because such delamination was not possible and the stem and bulb (tuber) part were not clearly distinguished.

In the tissue culture method, bulbous begonias are succulent, so they must be sterilized,
There were many problems with acclimatization, and there was a risk of mutations due to repeated culturing.

The method of dividing bulbs (tubers) was impractical because it was more likely to rot than the cut surface.

Short-day operation was carried out by drawing a blackout curtain inside the greenhouse, but due to mechanical problems, delays in dormancy due to high temperatures inside due to solar radiation, and increased cooling costs due to the large internal volume, bulbs (tubers) that last for many years It was practically impossible to harvest.

The present invention aims to increase the amount of cuttings collected, use the conventional cutting method, perform short-day operation with a simple device, and aim to propagate cloned seedlings and cloned bulbs (tubers) year-round.

[Means for Solving the Problems] In order to achieve the above object, in the propagation method of the present invention, in addition to the use of auxin as a rooting hormone, which has been used in conventional cuttings, as a germination hormone, With the combined use of cytokinin, a germination point is generated near the lower cut surface, and regardless of the presence or absence of a growing point, the remaining leaf pieces of the petiole with leaves, the main stem with leaves, and the leaf vein area are used as cuttings to collect cuttings. We aim to increase the yield of cuttings by eliminating restrictions on parts.
Cloned seedlings and cloned bulbs (tubers) are propagated throughout the year using a device that allows the nighttime temperature and sunlight hours to be set.

[Effects] As mentioned above, cuttings of bulbous begonias with rooting and germination hormones applied to the cut surface are treated with rooting and germination hormones.
Due to the action of cytokinin, numerous germination points are generated at the lower cut surface.

29 If the cuttings are placed under a long day with sunlight of at least 14 hours at an appropriate temperature, the germination point will begin to grow, take root, and become a seedling.

In addition, the cuttings treated as above were grown at a nighttime temperature of 18
If the cuttings are placed under a short daylight period of 8-10 hours at an appropriate temperature that does not exceed ℃, the cuttings will enter dormancy and begin to store nutrients from photosynthesis in the rooting site, forming a spherical shape with the germination point as the nucleus. become enlarged.

Therefore, it can be harvested by breaking off the bulb (tuber), which only contains stored nutrients.

During harvest, wounds on the bulbs (tubers) are protected by a corky covering using traditional curing techniques.

After harvesting the bulbs (tubers), cuttings can be re-planted, allowing another germination point to root and enlarge, allowing the bulbs (tubers) to be harvested. Cuttings can be used 3-4 times.

The size of the bulb (tuber) is determined by the number of days from the time of cutting to the time of harvest and the leaf area of the cutting.

[Example code] Examples will be described with reference to the drawings.

As shown in Figure 1, the cuttings were prepared, and a small amount of a mixture of naphthalene acetic acid as auxin and benzyladenine as cytokinin, increased to 1% with talc, was attached to the lower part of the cut surface, and 30 x 45 samples were prepared. Forty-five pieces were inserted into a box in the standard manner and placed on an electric trolley in a heat-insulating house as shown in Figure 2.

The cultivation equipment opens its doors at 4 p.m., and an electric trolley is run on the rails and placed on a lift inside a hangar surrounded by an insulated wall.The cultivation equipment is then lifted up by the movable hooks attached to the left and right posts, and then the lift is lowered. The next electric trolley is stored one after another. After all the electric trolleys are stored, the door is closed and the interior is kept at an appropriate temperature by the air conditioning system.

At around 8:00 a.m. the next morning, the door of the cultivation equipment was opened, the lift rose, and after loading the lowest electric trolley, the movable hook was removed and lowered, and it was rolled out into the greenhouse. Concentrated liquid fertilizer is mixed and watered at the same time as fertilization. After that, it is fed out one by one to the top stage, and once it is finished, the door is closed and the air conditioning system is stopped.

The above operations are performed every day by the sequential circuit of the control section.

Using the above device, when the scions were dug up after 90 days had passed, 2-3 bulbs (tubers) were obtained per scion, and a yield of about 120 bulbs per box was obtained. The size of the bulb (tuber) was about 5-15 mm in diameter.

The bulbs (tubers) were stored according to the standard method and then planted, and it was confirmed that they grew and bloomed normally, and were genetically uniform.

Furthermore, when the cuttings after harvesting the bulbs (tubers) were replanted, set in a cultivation device, and dug up again 90 days later, bulbs (tubers) of the same quality as the original were obtained.

[Effects of the Invention] Since the present invention is configured as described above, the following effects can be obtained.

1. Any part of a bulbous begonia with leaves attached can be used as a cutting, so the multiplication rate can be increased without putting a high burden on the mother tree.

2. Multiplication rate is high because multiple bulbs (tubers) can be harvested directly from cuttings.

3. Even when producing bulbs (tubers), the above-ground parts do not overgrow, making it easy to manage.

4. The same cuttings can be used several times to propagate bulbs (tubers), so the effects of 1.2.3 above can be further enhanced.

5. The cultivation equipment has a hangar surrounded by a heat insulating wall and can be stored in multiple stages, so the heating and cooling efficiency is good and it is easy to use over the years.

6. The box for cuttings is placed on an electric trolley and moved from place to place, so it can be used with a fixed irrigation system.

1-Insulated wall 3-lift 5-Heat insulation house 7-Electric trolley 9-Control device 2-Heating and cooling equipment 4-Movable hook 6-rail 8-door

Claims (3)

[Claims] (1) Cuttings are made from remaining leaf petioles, main stems, and veins of bulbous begonias where there are no buds, and a mixture of auxin and cytokinin, which are a type of plant hormone, is used as cuttings to reach the germination point. A propagation method characterized by producing, germinating, rooting, and producing seedlings. (2) A propagation method characterized by directly producing cloned bulbs (tubers) from cuttings without passing through the seedling stage of the preceding item (1), enlarging them, and harvesting them. (3) Increase the turnover rate of bulb (tuber) multiplication in the previous section (2),
In order to achieve longevity, labor saving, and energy saving, we use equipment consisting of insulated walls, air conditioning equipment, electric trolleys, rails, lifts, movable hooks, doors, warming houses, automatic watering equipment, and control units to control night temperature, A device characterized by being able to control sunlight hours.