JPH03289955A - 胚の凍結保存法 - Google Patents
胚の凍結保存法Info
- Publication number
- JPH03289955A JPH03289955A JP9064290A JP9064290A JPH03289955A JP H03289955 A JPH03289955 A JP H03289955A JP 9064290 A JP9064290 A JP 9064290A JP 9064290 A JP9064290 A JP 9064290A JP H03289955 A JPH03289955 A JP H03289955A
- Authority
- JP
- Japan
- Prior art keywords
- ice crystal
- inducing agent
- container
- adhesive
- crystal inducing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 18
- 210000001161 mammalian embryo Anatomy 0.000 title abstract description 16
- 238000007710 freezing Methods 0.000 title abstract description 10
- 230000008014 freezing Effects 0.000 title abstract description 10
- 239000013078 crystal Substances 0.000 claims abstract description 37
- 210000002257 embryonic structure Anatomy 0.000 claims abstract description 21
- 239000000203 mixture Substances 0.000 claims abstract description 20
- 238000007789 sealing Methods 0.000 claims abstract description 10
- 238000005138 cryopreservation Methods 0.000 claims description 21
- 239000000411 inducer Substances 0.000 claims description 17
- 230000001939 inductive effect Effects 0.000 abstract description 17
- 239000003795 chemical substances by application Substances 0.000 abstract description 16
- 239000004372 Polyvinyl alcohol Substances 0.000 abstract description 14
- 229920002451 polyvinyl alcohol Polymers 0.000 abstract description 14
- 239000000843 powder Substances 0.000 abstract description 13
- 239000000853 adhesive Substances 0.000 abstract description 12
- 230000001070 adhesive effect Effects 0.000 abstract description 12
- 241001465754 Metazoa Species 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 8
- JKFYKCYQEWQPTM-UHFFFAOYSA-N 2-azaniumyl-2-(4-fluorophenyl)acetate Chemical compound OC(=O)C(N)C1=CC=C(F)C=C1 JKFYKCYQEWQPTM-UHFFFAOYSA-N 0.000 abstract description 7
- 229910021612 Silver iodide Inorganic materials 0.000 abstract description 7
- 229940045105 silver iodide Drugs 0.000 abstract description 7
- 239000000126 substance Substances 0.000 abstract description 5
- 230000002411 adverse Effects 0.000 abstract description 4
- 230000006641 stabilisation Effects 0.000 abstract 3
- 238000011105 stabilization Methods 0.000 abstract 3
- 238000004321 preservation Methods 0.000 abstract 1
- 239000010902 straw Substances 0.000 description 17
- 239000000243 solution Substances 0.000 description 12
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- 229920000742 Cotton Polymers 0.000 description 8
- 238000002054 transplantation Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 239000003761 preservation solution Substances 0.000 description 3
- 230000000717 retained effect Effects 0.000 description 3
- 238000010257 thawing Methods 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- OMZSGWSJDCOLKM-UHFFFAOYSA-N copper(II) sulfide Chemical compound [S-2].[Cu+2] OMZSGWSJDCOLKM-UHFFFAOYSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 229920001651 Cyanoacrylate Polymers 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 235000010724 Wisteria floribunda Nutrition 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9064290A JPH03289955A (ja) | 1990-04-04 | 1990-04-04 | 胚の凍結保存法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP9064290A JPH03289955A (ja) | 1990-04-04 | 1990-04-04 | 胚の凍結保存法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH03289955A true JPH03289955A (ja) | 1991-12-19 |
| JPH0525496B2 JPH0525496B2 (enExample) | 1993-04-13 |
Family
ID=14004160
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP9064290A Granted JPH03289955A (ja) | 1990-04-04 | 1990-04-04 | 胚の凍結保存法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03289955A (enExample) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014223034A (ja) * | 2013-05-16 | 2014-12-04 | 三菱製紙株式会社 | 卵子又は胚のガラス化凍結保存用治具 |
| US10492487B2 (en) | 2013-05-16 | 2019-12-03 | Mitsubishi Paper Mills Limited | Vitrification-cryopreservation implement for cells or tissues |
| US10624335B2 (en) | 2014-10-23 | 2020-04-21 | Mitsubishi Paper Mills Limited | Tool for cryopreservation of cell or tissue and cryopreservation method |
| WO2024085220A1 (ja) * | 2022-10-21 | 2024-04-25 | 株式会社AnimoScience | ストロー管、ストロー、並びに精液又は受精卵の凍結保存方法 |
-
1990
- 1990-04-04 JP JP9064290A patent/JPH03289955A/ja active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014223034A (ja) * | 2013-05-16 | 2014-12-04 | 三菱製紙株式会社 | 卵子又は胚のガラス化凍結保存用治具 |
| US10492487B2 (en) | 2013-05-16 | 2019-12-03 | Mitsubishi Paper Mills Limited | Vitrification-cryopreservation implement for cells or tissues |
| US10624335B2 (en) | 2014-10-23 | 2020-04-21 | Mitsubishi Paper Mills Limited | Tool for cryopreservation of cell or tissue and cryopreservation method |
| WO2024085220A1 (ja) * | 2022-10-21 | 2024-04-25 | 株式会社AnimoScience | ストロー管、ストロー、並びに精液又は受精卵の凍結保存方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0525496B2 (enExample) | 1993-04-13 |
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