JPH0256076B2 - - Google Patents

Info

Publication number
JPH0256076B2
JPH0256076B2 JP60112287A JP11228785A JPH0256076B2 JP H0256076 B2 JPH0256076 B2 JP H0256076B2 JP 60112287 A JP60112287 A JP 60112287A JP 11228785 A JP11228785 A JP 11228785A JP H0256076 B2 JPH0256076 B2 JP H0256076B2
Authority
JP
Japan
Prior art keywords
plasmid
dna
ecor
gene
fragment
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
JP60112287A
Other languages
English (en)
Japanese (ja)
Other versions
JPS61271988A (ja
Inventor
Fumio Hishinuma
Rei Nishizawa
Kunio Kitada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP60112287A priority Critical patent/JPS61271988A/ja
Publication of JPS61271988A publication Critical patent/JPS61271988A/ja
Publication of JPH0256076B2 publication Critical patent/JPH0256076B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
JP60112287A 1985-05-27 1985-05-27 プラスミド Granted JPS61271988A (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP60112287A JPS61271988A (ja) 1985-05-27 1985-05-27 プラスミド

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP60112287A JPS61271988A (ja) 1985-05-27 1985-05-27 プラスミド

Publications (2)

Publication Number Publication Date
JPS61271988A JPS61271988A (ja) 1986-12-02
JPH0256076B2 true JPH0256076B2 (enrdf_load_stackoverflow) 1990-11-29

Family

ID=14582913

Family Applications (1)

Application Number Title Priority Date Filing Date
JP60112287A Granted JPS61271988A (ja) 1985-05-27 1985-05-27 プラスミド

Country Status (1)

Country Link
JP (1) JPS61271988A (enrdf_load_stackoverflow)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2061482T3 (es) * 1986-05-02 1994-12-16 Gist Brocades Nv Metodo para la identificacion de secuencias de señal secretora para sintesis de proteina extracelular en bacillus.

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0116201B1 (en) * 1983-01-12 1992-04-22 Chiron Corporation Secretory expression in eukaryotes
DK172738B1 (da) * 1983-04-07 1999-06-21 Chiron Corp Fremgangsmåde til at udtrykke modent insulin og DNA-struktur til anvendelse ved fremgangsmåden

Also Published As

Publication number Publication date
JPS61271988A (ja) 1986-12-02

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Legal Events

Date Code Title Description
EXPY Cancellation because of completion of term