JPH0229185B2 - - Google Patents
Info
- Publication number
- JPH0229185B2 JPH0229185B2 JP57190059A JP19005982A JPH0229185B2 JP H0229185 B2 JPH0229185 B2 JP H0229185B2 JP 57190059 A JP57190059 A JP 57190059A JP 19005982 A JP19005982 A JP 19005982A JP H0229185 B2 JPH0229185 B2 JP H0229185B2
- Authority
- JP
- Japan
- Prior art keywords
- signal
- section
- microparticle
- sample
- microcomputer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000003287 optical effect Effects 0.000 claims description 32
- 239000011859 microparticle Substances 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 12
- 238000001514 detection method Methods 0.000 claims description 11
- 238000004364 calculation method Methods 0.000 claims description 10
- 239000000725 suspension Substances 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 8
- 239000013307 optical fiber Substances 0.000 claims description 4
- 230000010287 polarization Effects 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 239000000835 fiber Substances 0.000 claims description 2
- 238000001917 fluorescence detection Methods 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 32
- 238000010586 diagram Methods 0.000 description 16
- 238000012545 processing Methods 0.000 description 13
- 239000000523 sample Substances 0.000 description 12
- 238000005259 measurement Methods 0.000 description 8
- 238000004458 analytical method Methods 0.000 description 7
- 230000028161 membrane depolarization Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 4
- 238000002875 fluorescence polarization Methods 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000013500 data storage Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000012489 doughnuts Nutrition 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1434—Optical arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57190059A JPS5979835A (ja) | 1982-10-29 | 1982-10-29 | 微小粒子分析装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57190059A JPS5979835A (ja) | 1982-10-29 | 1982-10-29 | 微小粒子分析装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5979835A JPS5979835A (ja) | 1984-05-09 |
| JPH0229185B2 true JPH0229185B2 (de) | 1990-06-28 |
Family
ID=16251656
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57190059A Granted JPS5979835A (ja) | 1982-10-29 | 1982-10-29 | 微小粒子分析装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5979835A (de) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4989977A (en) * | 1985-07-29 | 1991-02-05 | Becton, Dickinson And Company | Flow cytometry apparatus with improved light beam adjustment |
| US4781460A (en) * | 1986-01-08 | 1988-11-01 | Coulter Electronics Of New England, Inc. | System for measuring the size distribution of particles dispersed in a fluid |
-
1982
- 1982-10-29 JP JP57190059A patent/JPS5979835A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5979835A (ja) | 1984-05-09 |
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