JPH01172338A - Preventive and remedy for swine dysentery - Google Patents

Abstract

PURPOSE:To obtain the title preventive and remedy having no problems of occurrence of resistant bacteria and side effects and no fear of influence of remaining agent on human body, comprising a crude drug such as root and rhizome of Glycyrrhiza glabra Linne var. glandulifera Regel et Herder, rhizome of Coptis japonica Makino or root of Scutellarla baicalensis Georgi as an active ingredient. CONSTITUTION:The title preventive and remedy comprising a crude drug selected from the group consisting of root and rhizome of Glycyrrhiza glabra Linne var. glandulifera Regel et Herder, rhizome of Coptis japonica Makino, root of Scutellarla baicalensis Georgi, bark of Myrica rubra Siebold et Zuccarini, Aloe arborescens Mill. var. ratalensis Bergel., gall of Bostaurus domesticuls Gmelin, stems and leaves of Isodon japonica Hara and Galla rhosis as an active ingredient. The crude drug is administered in the form of raw powder, essence extracted with an organic solvent or water or an active ingredient of Galla rhois obtained from the essence and a dose thereof is 0.001mg-100g calculated as weight of raw powder per kg weight daily.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Application Field] The present invention relates to a prophylactic and therapeutic agent and a prophylactic and therapeutic method for swine dysentery.

More specifically, the present invention relates to the bacterium Treponeiahy.
The present invention relates to preventive and therapeutic agents and preventive and therapeutic methods using crude drugs for swine dysentery, which is an infectious disease of pigs caused by P. odysen Leriae.

[Prior Art] Swine dysentery is one of the bacterial infectious diseases of pigs, and the pathogen is Treponeia hyodysenterlae.
It is. It causes delayed growth of pigs, reduced feed efficiency, etc., and tends to become endemic when it occurs, causing great industrial damage to the pig farming industry, and among the many diseases of pigs, it is difficult to establish control measures. It is one of the most urgent diseases.

This disease is an acute or chronic intestinal infection mainly caused by the excretion of mucus and bloody diarrhea. The affected side exhibits symptoms of dysentery, and the mortality rate is approximately 5-25%. Chronic cases generally have a poor prognosis, and even if death is avoided, growth stops.

Despite the fact that it is such a serious infectious disease, there are almost no effective countermeasures, and although measures such as proper breeding management are taken, in reality, it is often impossible to prevent it.

Furthermore, in order to prevent and treat this disease, for example, methods have been used in which antibiotics and other antibacterial agents are administered together with feed. However, in recent years, there has been a growing trend that it is not appropriate to administer antibiotics to animals such as livestock due to problems such as the emergence of resistant bacteria and the persistence of antibiotics in animals. Therefore, the development of preventive and therapeutic methods and preventive and therapeutic agents that do not use antibiotics is desired.

[Means for Solving the Problems] As a result of research to solve the above problems, the present inventors discovered that certain herbal medicines have the effect of suppressing this disease, and completed the present invention. Ta.

That is, an object of the present invention is to provide a preventive and therapeutic agent for swine dysentery and a method for preventing and treating swine dysentery.

The present invention provides a prophylactic and therapeutic agent for swine dysentery containing one or more herbal medicines selected from the group consisting of licorice, scutellariae, scutellariae, scutellariae, aloe, gyutan, chilifolia, and burdock. .

The present invention also relates to crude drugs in the form of bulk powders, organic solvents, mixtures of organic solvents and water, or crude drug extracts obtained by extraction with water;
Alternatively, the present invention provides the above-mentioned preventive and therapeutic agent for swine dysentery containing an active ingredient of herbal medicine.

Furthermore, the present invention provides a method for the prevention and treatment of swine dysentery, which comprises administering an effective amount of one or more crude drugs selected from the group consisting of licorice, orensis, scutellariae, scutellariae, aloe, gyutan, chiliflower, and burdock. The present invention provides a method.

The present invention also relates to the prevention and treatment of swine dysentery, which comprises administering crude drugs in the form of bulk powders, organic solvents, mixtures of organic solvents and water, crude drug extracts obtained by extraction with water, or active ingredients of crude drugs. It provides a treatment method.

The present invention also provides a method for preventing and treating swine dysentery, which comprises administering a crude drug in feed.

Licorice (licorice) is called daylily (Glyeyrrhlz)
aglabra LInnA var, grand
ulifera Regel et Herder) roots and rhizomes as they are or with the cork skin removed. This product contains ingredients such as glycyrrhizin, liquiritin, glucose, and asparagine, and is used as a relaxing, antispasmodic, and phlegm medicine for humans.

Ouren (Yellow Ren) is ouren (Coptisjapo).
It is made by burning off the fine roots from the rhizomes of Nica Makino. This product contains ingredients such as berberine and palmatine, and is used as a bitter and intestinal medicine for humans.

Scutel I (i29) is Scutel I
This is the root of the plant (Arlabalcalensis Georgl) with the cork skin removed. This product contains orgonin and picalin as ingredients, and is used as an anti-inflammatory and antipyretic drug for humans.

Youbaihi (fried plum skin) is a Japanese bayberry (Myrlcaru).
bra 5iebold at Zuccarini)
It is the bark of the tree. This product contains tannins, myricetin, myricitrin, etc., and is used as an astringent for diarrhea in humans, and is also effective against bruises.

Aloe is Aloe arborescens.
Mill.

var, raLalensis Bergel,). This product contains barbaloin, aloe emodin, etc. as ingredients, and has been used as a bitter stomachic or laxative for humans in the Middle East and Europe since ancient times.

Gyutan (beef tallow) is derived from cow (Bos taurus).
The gallbladder of the omes LIcus GIlelln) is hung to dry in the shade, or the bile duct is incised and the bile is stored in a container in a sealed container. This product contains sodium cholate, mucoprotein, fatty acids, etc. as ingredients, and has been used for a long time to treat conditions such as jaundice, constipation, diabetes, and hemorrhoid healing in humans.

Enmeiso (Enmeiso) is called Hikiokoshi (Isodon).
japonica Hara) or Isodon trichocarpus Ku
These are the stems and leaves of do).

This product contains plectrantin and enmain as ingredients, and is used as a bitter medicine for human stomach health to treat indigestion, anorexia, abdominal pain, etc.

Gobaishi (Gobaishi) is the aphid (Nurudenomimifushia aphid)
Melaphis chlnensls J, Be1
The sting of Rhus java
It is a gall produced in the leaves of nica L [nnj), and contains tannin, gallic acid, fat, resin, etc. as components. It is used as a raw material for the production of five-fold tincture, tannic acid, gallic acid, and pyrogallol.

As mentioned above, each of the herbal medicines used in the present invention, such as licorice, orensis, scutellariae, scutellariae, aloe, gyutan, japonica, and gobaishi, can be used alone or
or in combination with products that have traditionally been used for the prevention and treatment of human diseases, but these are used for the purpose of preventing and treating diseases in pigs, especially for the purpose of preventing and treating infectious diseases in pigs. No attempt had ever been made to do so.

The present inventors have conducted various studies focusing on the medicinal effects of the above-mentioned herbal medicines, and have found that swine dysentery, that is, pigs are affected by the bacterium Trepone.
The present invention was completed based on the discovery that administering one or more of the above-mentioned herbal medicines in combination is extremely effective for the prevention and treatment of diseases caused by intestinal infection with M. ma hyodyscntcriae.

That is, as described below, the above-mentioned specific types of herbal medicines and their extracts are effective against bacterial T.
It has been revealed that TrepOnella hyodysentertae has bacteriostatic and bactericidal effects specifically against TrepOnella hyodysentertae.
As a result of the inhibitory effect on Treponema hy.
It was possible to prevent and cure swine dysentery, a disease of pigs caused by P. odysenterlae.

The effectiveness of the above-mentioned specific herbal medicines and their extracts against this disease unique to pigs was unexpected and was revealed for the first time by the present inventors.

These crude drugs can be administered directly in the form of raw powder, extracts, or active ingredients of crude drugs, or they can be added to feed, or solutions, dispersions, semi-solids, powders, and molded preparations. It is used in formulations such as infusion preparations, injection preparations, etc.

Extracts can be obtained by solvent extraction, for example using water, an organic solvent, or a mixture of water and an organic solvent, and then left as is, or
It can be used concentrated, diluted, or with the solvent removed.

Examples of organic solvents include methanol, ethanol, n
-Propertool, n-butanol, acetone, ethyl acetate, ether, methylene chloride, chloroform, benzene, carbon tetrachloride, petroleum ether, etc. are used, and methanol and ethanol are particularly preferred. These organic solvents can be used alone or as a mixture of two or more.

This solvent extraction can be carried out by cold soaking or digesting the herbal medicine in these solvents. 15-25℃ for cold soaking
Digestion can be carried out at a temperature range of 35-45°C. The extraction time will vary depending on the extraction temperature, but may be approximately 5 days or until the soluble components are fully dissolved in the shell.

The active ingredients of herbal medicine can be obtained by, for example, shaking the above-mentioned extract using various solvents, extracting the fraction that transfers to the solvent, and distilling off the solvent, which is used as the effective fraction, that is, the white active ingredient. You can also do that.

Solvents used in this case include nonpolar solvents such as lower aliphatic ethers (diethyl ether, ethyl methyl ether, etc.); lower halogen alkanes (chloroform, ethyl methyl ether, etc.);
Examples include dichloroethane, dibromoethane, etc.); benzenes (benzene, toluene, xylene, etc.); and other petroleum solvents (petroleum ether, petroleum benzene, ligroin, etc.).

In addition, the remaining aqueous solution from which this effective fraction was extracted is extracted by shaking using water and a kind of organic solvent saturated with water, washed with water, and transferred to the organic solvent layer that is the residue. A product obtained by distilling off both solvents can also be used as an effective fraction, that is, as an active ingredient. This effective fraction has different physicochemical properties and different detected components from the above-mentioned effective fraction.

The dose is 0.001mg/converted to the weight of the raw drug powder.
body ffi) cg/day ~ 100. /kg body weight/day.

This amount is different when used as a prophylactic agent and when used as a therapeutic agent - the dose in the shell is higher in the latter case.

The administration method can be oral or parenteral administration, and examples of parenteral administration include intramuscular administration, intraperitoneal administration, transdermal administration, nasal administration, intravenous administration, and the like.

[Effects of the Invention] As detailed above, the present invention provides an effective preventive and therapeutic agent and an effective preventive and therapeutic method for swine dysentery.

Since the prophylactic and therapeutic agent of the present invention uses herbal medicines or extracts obtained from them as herbal medicines, there is no problem of the development of resistant bacteria or side effects associated with antibiotics, etc., and it can be administered with confidence.

Furthermore, since there is no concern about the effects of drug residue on the human body, it is suitable as a drug for preventing and treating swine dysentery in meat pigs.

Furthermore, the disease can be prevented and treated at a lower cost than when using antibiotics or the like.

The present invention will be explained in more detail below using test examples and examples.

Test Example I Treponesa] + yodysenterjae
The susceptibility to various herbal medicines was investigated.

Various crude drugs were added to a 50% ethanol aqueous solution in an amount seven times the amount of the crude drug, extracted for 16 hours, and then extracted with alcohol to obtain an alcohol extract.

The extract was infiltrated into a disk with a diameter of 8 mm at a dose of 25 μl and dried. Transfer this disk to the above Treponema
hyodysenterlae strain was placed on a blood agar medium and cultured at 37° C. for 18 hours, the diameter of the inhibition circle was measured.

Next, the test results are shown in Table 1.

(Leaving space below) Table 1 Licorice 20 Corn 32 Scutellariae 14 Yo-Ubaihi 9 Aloe 32 Gyutan 20 Chillies 15 Gobaishi 13 Example 1 Basic feed corn 450 parts by weight Milo
31O soybean meal
146//Na Ta Ne Kasu
30 Fish powder
15. Meat bone powder
10ffi yellow grease 1g
” Calcium carbonate 5 Calcium phosphate 8 Food
Salt 3 Premix 5 2% by weight of Oren powder was added to the basic feed to prepare a test feed.

100 healthy pigs, 60 days old, were fed the test feed ad libitum until they were 180 days old, and during this period, the number of pigs suffering from diarrhea due to field infection and the number of days were observed.

The control group was treated in the same manner as above except that basal feed was given instead of the test feed.

As a result, in the test area, the number of pigs with diarrhea due to swine dysentery was 2/1.
00 cows, and the total number of days with diarrhea was 8 days / 1200.
In contrast, in the control area, 36/100 pigs had diarrhea due to swine dysentery, and the total number of pigs with diarrhea was 221/12,000 days.

Examples 2 to 8 The same procedure as in Example 1 was carried out except that instead of Oren in Example 1, 1% of each of Licorice, Scutellariae, Scutellariae, Aloe, Gyutan, Trifolium, and Cucumber were added to the basal feed at 1% and used as the test feed. I did it.

Next, the test results are shown in Table 2.

(Left below) Example 9 Licorice, Oriental lily, Scutellaria japonica, Scutellaria sinensis, Aloe vera,
1 kg of each of Gyutan, Enmeisou, and Gobaishi was taken, added to 10 g of water, and extracted at 100'C for 1 hour. Each water extract was diluted 1000 times to obtain an extract.

100 60-day-old pigs were used in each test group, and the extract was fed in place of water, and the pigs were kept until they were 100 days old.

Note that the basal feed used in Example 1 was used as the feed.

The control group received only water instead of the extract.

Next, during the test period, the number of animals with diarrhea due to swine dysentery and the number of days with diarrhea during field infection were observed. As a result, the number of animals suffering from diarrhea due to field infection due to swine dysentery was smaller in the test plot than in the control plot, and the total number of days of diarrhea was also smaller.

Claims (1)

[Scope of Claims] 1) A prophylactic and therapeutic agent for swine dysentery, which contains one or more crude drugs selected from the group consisting of licorice, orensis, scutellariae, scutellariae, aloe, gyutan, trifolium, and burdock. 2) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is in the form of bulk powder. 3) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is an organic solvent, a mixture of water and an organic solvent, a crude drug extract extracted with water, and/or a crude drug active ingredient obtained therefrom. 4) The prophylactic and therapeutic agent according to claim 1, wherein the crude drug is blended into feed. 5) A method for preventing and treating swine dysentery, which comprises administering an effective amount of one or more herbal medicines selected from the group consisting of licorice, scutellariae, scutellariae, scutellariae, aloe, staghorn, scutellariae, and scutellariae. 6) Claim 5 in which the crude drug is used in the form of bulk powder
Prevention and treatment methods described in Section. 7) The prevention and treatment method according to claim 5, wherein the herbal medicine is an organic solvent, a mixture of water and an organic solvent, a herbal medicine extract extracted with water, and/or an active herbal medicine ingredient obtained therefrom. 8) The prevention and treatment method according to claim 5, wherein the crude drug is administered by mixing it with feed.